Number of found documents: 307
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Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions. Keywords: enzyme production; bacterial production; submergeed aerobic cultivation Available at various institutes of the ASCR
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz

Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
Mikrobiologický ústav, 2014

Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
Kozáková, Hana
2014 - English
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete microflora. Keywords: flies; lactobacili; mice Available at various institutes of the ASCR
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development

We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete ...

Kozáková, Hana
Mikrobiologický ústav, 2014

Transfer of technology for production of AEH from Sphingomonas sp
Kyslíková, Eva; Palyzová, Andrea; Marešová, Helena; Štěpánek, Václav; Zahradník, Jiří; Bečka, Stanislav; Dobišová, Marie; Kyslík, Pavel
2014 - English
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross linked enzyme agregate Keywords: Alpha aminoacid ester hydrolase; heterologous expression; stirred bioreactor Available at various institutes of the ASCR
Transfer of technology for production of AEH from Sphingomonas sp

Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross ...

Kyslíková, Eva; Palyzová, Andrea; Marešová, Helena; Štěpánek, Václav; Zahradník, Jiří; Bečka, Stanislav; Dobišová, Marie; Kyslík, Pavel
Mikrobiologický ústav, 2014

Vývoj technologií pilotní přípravy laktamáz a dalších enzymů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of the production of some enzymes for pharmaceutical industry. Cultivation technology and down-stream processing. Keywords: enzyme production; bacterial production; submerged aerobic cultivation; biotechnology Available at various institutes of the ASCR
Vývoj technologií pilotní přípravy laktamáz a dalších enzymů

Development and optimization of the production of some enzymes for pharmaceutical industry. Cultivation technology and down-stream processing.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
Mikrobiologický ústav, 2014

BATCH BIOREACTORS: WASTE WATER ENDOCRINE DISRUPTOR BIODEGRADATION BY WHITE ROT FUNGI
Křesinová, Zdena; Cajthaml, Tomáš
2013 - English
Endocrine disruptors (EDs) are members of a broad class of environmental organopollutants which are present in the environment. Due to the widespread presence in the surface water and wastewater, and their negative endocrine disrupting activity even at very low concentration, these EDs have received an increased attention in water quality management and health care. EDs encompass a variety of substances, including mammalian hormones (estrogens, androgens) and anthropogenic chemicals Keywords: organopollutants Available at various institutes of the ASCR
BATCH BIOREACTORS: WASTE WATER ENDOCRINE DISRUPTOR BIODEGRADATION BY WHITE ROT FUNGI

Endocrine disruptors (EDs) are members of a broad class of environmental organopollutants which are present in the environment. Due to the widespread presence in the surface water and wastewater, and ...

Křesinová, Zdena; Cajthaml, Tomáš
Mikrobiologický ústav, 2013

TEMPERATURE INFLUENCE ON DESORPTION AND DETERMINATION OF BIOACCESSIBLE PAHS
Čvančarová, Monika; Pokorná, Lenka; Velebová, R.; Najmanová, P.; Cajthaml, Tomáš
2013 - English
Hydrophobic organic pollutants tend to adsorb onto soil particles which can cause a significant decrease in their bioaccessible fractions. Definition of the bioaccessibility is currently not unified; however it represents a part of total PAHs amount which is potentially available for biological processes. It is an important parameter which can be used for prediction of microbial degradation and environmental risk evaluation. This paper studies desorption behavior of PAHs at 20 and 60˚C. The desorption of PAHs from long-term contaminated soils was evaluated using resin Amberlite XAD- 2. A two-phase desorption model was used to calculate the desorption kinetic curves and F fractions which represent bioaccessible amount of PAHs. The results showed that the desorption of PAHs was significantly influenced by temperature. The greatest differences in the desorption behavior were observed at 5–6 ring PAHs. We compared the F fractions and the rate constants of individual PAHs which were determined at 20 and 60˚C. The results were also compared with sequential supercritical fluid extraction which has been already used for prediction of microbial degradation. Keywords: pahs Available at various institutes of the ASCR
TEMPERATURE INFLUENCE ON DESORPTION AND DETERMINATION OF BIOACCESSIBLE PAHS

Hydrophobic organic pollutants tend to adsorb onto soil particles which can cause a significant decrease in their bioaccessible fractions. Definition of the bioaccessibility is currently not unified; ...

Čvančarová, Monika; Pokorná, Lenka; Velebová, R.; Najmanová, P.; Cajthaml, Tomáš
Mikrobiologický ústav, 2013

ENDOCRINE DISUPTIVE CHEMICALS: MECHANISMS OF WHITE ROT FUNGI BIODEGRADATION
Cajthaml, Tomáš; Křesinová, Zdena; Čvančarová, Monika; Maternová, H.; Filipová, A.
2013 - English
The main aim of this study was to elucidate mechanisms involved in EE2 and other EDs compounds degradation by white rot fungi. Results showed simultaneous involvement of ligninolytic enzymes and cytochrome P-450 in biodegradation. The present results indicate the advantage of utilization of WRF cultures for organopollutants degradation. Keywords: biodegradation Available at various institutes of the ASCR
ENDOCRINE DISUPTIVE CHEMICALS: MECHANISMS OF WHITE ROT FUNGI BIODEGRADATION

The main aim of this study was to elucidate mechanisms involved in EE2 and other EDs compounds degradation by white rot fungi. Results showed simultaneous involvement of ligninolytic enzymes and ...

Cajthaml, Tomáš; Křesinová, Zdena; Čvančarová, Monika; Maternová, H.; Filipová, A.
Mikrobiologický ústav, 2013

A NOVEL BIOAUGMENTATION APPROACH IN BIODEGRADATION OF ORGANIC POLLUTANTS IN CONTAMINATED SOIL
Lhotský, O.; Filipová, Alena; Innemanová, P.; Velebová, R.; Cajthaml, Tomáš
2013 - English
Polycyclic aromatic hydrocarbons (PAHs) belong among organic compounds that accumulate in the environment mainly resulting from human activities. Because of their potentially negative effects on human health, methods of their removal from the environment are intensively studied. In this work, a biodegradation method of bioaugmentation based on sequential stepwise mixing of inoculated soil with non inoculated soil was used. The subject of this work was an application of a bacterial consortium on a real contaminated (7399 mg.kg-1 of PAHs) soil. 8 bacterial strains isolated from other contaminated sites were inoculated. DNA was isolated from the samples during the inoculation process and the degradation of PAHs was monitored after 4 months. Keywords: soil Available at various institutes of the ASCR
A NOVEL BIOAUGMENTATION APPROACH IN BIODEGRADATION OF ORGANIC POLLUTANTS IN CONTAMINATED SOIL

Polycyclic aromatic hydrocarbons (PAHs) belong among organic compounds that accumulate in the environment mainly resulting from human activities. Because of their potentially negative effects on human ...

Lhotský, O.; Filipová, Alena; Innemanová, P.; Velebová, R.; Cajthaml, Tomáš
Mikrobiologický ústav, 2013

Development of recombinant Pichia pastoris strain expressing synthetic gene of npga by genetic cloning
Marešová, Helena; Maršálek, Jaroslav; Bečka, Stanislav; Kyslík, Pavel
2012 - English
Cloning of synthetic npga gene into Pichia pastoris. Development of high expression system for penicillin acylase in a stirred bioreactor. Isolation and characterization of the enzyme. Keywords: penicillin acylase; heterologous expression; stirred bioreactor Available at various institutes of the ASCR
Development of recombinant Pichia pastoris strain expressing synthetic gene of npga by genetic cloning

Cloning of synthetic npga gene into Pichia pastoris. Development of high expression system for penicillin acylase in a stirred bioreactor. Isolation and characterization of the enzyme.

Marešová, Helena; Maršálek, Jaroslav; Bečka, Stanislav; Kyslík, Pavel
Mikrobiologický ústav, 2012

Microalgae Chlorella sp. as an Alternative Source of Fermentable Sugars
Maršálková, B.; Širmerová, M.; Kuřec, M.; Brányik, T.; Brányiková, Irena; Melzoch, K.; Zachleder, Vilém
2010 - English
The aim of this work was to optimize the enzymatic hydrolysis of starch originating from microalgae (34.0 +/- 1.2% wt. starch in the dry algal biomass) grown on flue gas from waste incineration plant and to study the influence cell disintegration method on the yield of hydrolysis. Apart from the starch, the possibility of cellulose usage from microalgae cell wall as a further source of fermentable sugars was studied and preliminary results are presented. Keywords: Flue-gas; Biofuels; Photobioredactor Available at various institutes of the ASCR
Microalgae Chlorella sp. as an Alternative Source of Fermentable Sugars

The aim of this work was to optimize the enzymatic hydrolysis of starch originating from microalgae (34.0 +/- 1.2% wt. starch in the dry algal biomass) grown on flue gas from waste incineration plant ...

Maršálková, B.; Širmerová, M.; Kuřec, M.; Brányik, T.; Brányiková, Irena; Melzoch, K.; Zachleder, Vilém
Mikrobiologický ústav, 2010

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