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Determination of stability constants of antamanide complexes with sodium and potassium ions by affinity capillary electrophoresis
Pangavhane, Sachin; Böhm, S.; Makrlík, E.; Ruzza, P.; Kašička, Václav
2018 - English
Affinity capillary electrophoresis (ACE) was applied for quantitative investigation of the interactions between important biomolecule, cyclic decapeptide antamanide (AA), cycl[-Val(1)-Pro(2)-Pro(3)-Ala(4)-Phe(5)-Phe(6)-Pro(7)-Pro(8)-Phe(9)-Phe(10)-], and sodium and potassium cations in methanol. Apparent stability constants of AA complexes with these alkali metal ions were determined from the dependence of effective mobility of AA on the concentration of these ions in the background electrolyte by the non-linear regression analysis. The AA complexes with Na+ and K+ ions were found to be relatively weak, with the apparent stability constants 343 L/mol and 35 L/mol, respectively.
Keywords:
affinity capillary electrophoresis; antamanide; binding constant
Available at various institutes of the ASCR
Determination of stability constants of antamanide complexes with sodium and potassium ions by affinity capillary electrophoresis
Affinity capillary electrophoresis (ACE) was applied for quantitative investigation of the interactions between important biomolecule, cyclic decapeptide antamanide (AA), ...
Separation of rotamers of 5-nitrosopyrimidines by capillary electrophoresis
Štěpánová, Sille; Procházková, Eliška; Čechová, Lucie; Žurek, Jiří; Janeba, Zlatko; Dračínský, Martin; Kašička, Václav
2018 - English
A new capillary electrophoresis (CE) method was developed for the separation of a new type of stereoisomers - rotamers of polysubstituted 5-nitrosopyrimidine derivatives. Partial separation of some rotamers was obtained in sodium borate, pH 9.3, or in Tris-phosphate, pH 2.2, background electrolytes (BGEs) free of cyclodextrins (CDs). However, the best, baseline separations of the rotamers of 5-nitrosopyrimidines were achieved in sodium borate BGE, pH 9.3, containing 20 mg/mL beta-CD as stereoselector, or in Tris-phosphate BGE, pH 2.2, with carboxymethyl-beta-CD as stereoselector.
Keywords:
rotamers; 5-nitrosopyrimidines; capillary electrophoresis
Available at various institutes of the ASCR
Separation of rotamers of 5-nitrosopyrimidines by capillary electrophoresis
A new capillary electrophoresis (CE) method was developed for the separation of a new type of stereoisomers - rotamers of polysubstituted 5-nitrosopyrimidine derivatives. Partial separation of some ...
Iontové zdroje pro LC/MS
Cvačka, Josef
2018 - Czech
Byly popsány iontové zdroje, které se používají pro spojení kapalinového chromatografu s hmotnostním spektrometrem. The ion sources used for conection of liquid chromatograph and the mass spectrometer were described.
Keywords:
mass spectrometry; ionization
Available at various institutes of the ASCR
Iontové zdroje pro LC/MS
Byly popsány iontové zdroje, které se používají pro spojení kapalinového chromatografu s hmotnostním spektrometrem....
Determination of Bitrex in conifer needles using capillary electrophoresis
Jaklová Dytrtová, Jana; Jakl, M.; Konášová, Renáta; Kašička, Václav
2018 - English
Two extraction procedures have been developed for the sample preparation regarding the determination of Bitrex (Denatonium benzoate) on the surface of conifer needles (1) and in the biomass of the chopped conifer needles (2). The content of the Bitrex in the extracts was determined by capillary electrophoresis with UV-absorption photometric detection. The recovery of Bitrex from the needle surface is 90%, however the procedure designed for determination of Bitrex from needles is not sufficient, because the recovery is only 30 %.
Keywords:
denatonium benzoate; needle extracts; capillary electrophoresis
Available at various institutes of the ASCR
Determination of Bitrex in conifer needles using capillary electrophoresis
Two extraction procedures have been developed for the sample preparation regarding the determination of Bitrex (Denatonium benzoate) on the surface of conifer needles (1) and in the biomass of the ...
Estimation of binding constants of associates of Ru(II) and Fe(II) polypyridyl complexes with single-isomer sulfated cyclodextrins by capillary electrophoresis
Sázelová, Petra; Koval, Dušan; Severa, Lukáš; Teplý, Filip; Kašička, Václav
2018 - English
Capillary electrophoresis (CE) was applied for enantioseparations of [Ru(bpy)3]2+, [Ru(phen) 3]2+ and [Fe(phen) 3]2+ complexes and for the estimation of binding constants of their associates with selected single-isomer sulfated cyclodextrins (SCDs). The apparent binding constants of the complexes of [Ru(bpy)3]2+, [Ru(phen)3]2+, and [Fe(phen)3]2+ enantiomers with the above SCDs were determined from the dependence of their effective electrophoretic mobilities on the concentration of the SCDs in the background electrolyte. Calculated apparent binding constants were found to be smaller than those obtained for randomly sulfated SCDs.
Keywords:
polypyridyl complexes; stability constant; capillary electrophoresis
Available at various institutes of the ASCR
Estimation of binding constants of associates of Ru(II) and Fe(II) polypyridyl complexes with single-isomer sulfated cyclodextrins by capillary electrophoresis
Capillary electrophoresis (CE) was applied for enantioseparations of [Ru(bpy)3]2+, [Ru(phen) 3]2+ and [Fe(phen) 3]2+ complexes and for the estimation of binding constants of their associates with ...
Penconazole and its behaviour in the presence of copper
Jakl, M.; Jaklová Dytrtová, Jana
2018 - English
Penconazole is a fungicide frequently used in agriculture. Its possible impact to (non-)target species is influenced by its interactions e.g. with copper, typically present in soil as an ion (or in week complexes) or in bodies, as a part of enzymes. This study brings the information potentially useful in the evaluation of penconazole fate in the environment. The studies based on the electrospray gas phase experiments provide an estimate of the penconazole reactivity in copper presence including its possible degradation pathways.
Keywords:
triazoles; Cu(II); Cu(I); mass spectrometry; ESI-MS; stability; degradation
Available at various institutes of the ASCR
Penconazole and its behaviour in the presence of copper
Penconazole is a fungicide frequently used in agriculture. Its possible impact to (non-)target species is influenced by its interactions e.g. with copper, typically present in soil as an ion (or in ...
Development of a tube nebulizer for microflow atmospheric pressure chemical ionization mass spectrometry
Strmeň, Timotej; Vrkoslav, Vladimír; Pačes, Ondřej; Cvačka, Josef
2017 - English
A novel nebulizer for microflow atmospheric pressure chemical ionization mass spectrometry was developed. It consisted of a tube with analyte-transporting fused silica capillary inside. The terminal part of the tube was resistively heated using a coiled resistance wire. Optimal nebulizer parameters and construction setup were investigated to fully demonstrate its versability. Corundum appeared to be a more suitable material than quartz for fabrication of the tube nebulizer. The lowest analyte flow rate investigated was 1.0 mul/min with an excellent signal stability reached.
Keywords:
mass spectrometry; micro-APCI; tube nebulizer
Available at various institutes of the ASCR
Development of a tube nebulizer for microflow atmospheric pressure chemical ionization mass spectrometry
A novel nebulizer for microflow atmospheric pressure chemical ionization mass spectrometry was developed. It consisted of a tube with analyte-transporting fused silica capillary inside. The terminal ...
Derivatizace v hmotnostní spektrometrii pro určování struktury
Cvačka, Josef
2017 - Czech
Přehled hmotnostně-spektrometrických přístupů pro určování poloh dvojných vazeb a methylových větvení u alifatických řetězců. An overview of mass spectrometric approaches for determining the positions of double bonds and methyl branches in aliphatic chains.
Keywords:
mass spectrometry; derivatization
Available at various institutes of the ASCR
Derivatizace v hmotnostní spektrometrii pro určování struktury
Přehled hmotnostně-spektrometrických přístupů pro určování poloh dvojných vazeb a methylových větvení u alifatických řetězců....
ICP-OES s elektrotermickou vaporizací (ETV) a příklady využití metody při přímé analýze různých biologických materiálů
Matějková, Stanislava
2017 - Czech
Optická spektrometrie s indukčně vázaným plasmatem a elektrotermickou vaporizací (ETV-ICP-OES) je metodou umožňující jednoduché a rychlé přímé stanovení makro-, mikro- i stopových prvků v pevných i kapalných vzorcích bez předchozí úpravy. Metoda snižuje riziko kontaminace vzorků a vzhledem k vysoké efektivitě přenosu analytů do plasmatu je možno provádět analýzu z velmi malého množství vzorků (jednotky mg). V naší laboratoři byla vyvinuta metoda pro sledování hladiny a chování derivátů karboranů v organismu na základě stanovení koncentrace boru a kobaltu v malých množstvích myších tkání (5 – 10 mg séra, jater mebo mozku). Dále byly vyvinuty metody pro rychlou simultánní analýzu makro- i mikroelementů v rostlinném materiálu a stanovení potenciálně škodlivých prvků v tělech pískomilného hmyzu. Solid sampling electrothermal vaporization (ETV) inductively coupled plasma optical emission spectroscopy (ICP-OES) enable simple and rapid multielemental analysis of major, minor and trace elements without the need for pre-treatment of solid or liquid samples. By comparison with more common ICP-OES combined with a solution nebulization, the risk of contamination of analysed samples is reduced. The detection limits and the sample quantity needed for analysis via ETV-ICP-OES decrease as a result of the high-efectivity transport of the sample aerosol into the ICP torch. The connection of ETV unit to simultaneous ICP-OES allows us the fast direct and fully automatised analysis of numerous samples. The technique enable usually the combined calibration with home-made or certified solid and aqueous standards. We developed the methodology for tracking the carboran-based candidate-drugs time dependent concentration and for their pharmacokinetic evaluation. The method is based on direct boron or together boron and cobalt contents direct estimation in little amounts of fresh frozen mice tissues (5 – 10 mg of serum, brain or liver). We created also methods for multielement analysis of plant material and for determination of potentionaly harmful elements in insect bodies.\n
Keywords:
ETV-ICP-OES; biological samples; multielemental analysis
Available at various institutes of the ASCR
ICP-OES s elektrotermickou vaporizací (ETV) a příklady využití metody při přímé analýze různých biologických materiálů
Optická spektrometrie s indukčně vázaným plasmatem a elektrotermickou vaporizací (ETV-ICP-OES) je metodou umožňující jednoduché a rychlé přímé stanovení makro-, mikro- i stopových prvků v pevných i ...
Od běžných ke speciálním aplikacím zobrazovací hmotnostní spektrometrie
Vrkoslav, Vladimír
2017 - Czech
Bylo popsáno využití desorpčních ionizačních technik pro zobrazování distribuce molekul v typických i nestandardních aplikacích. The use of desorption ionization techniques for the distribution of molecules in both typical and non-standard applications was described.
Keywords:
MSI; molecular imaging
Available at various institutes of the ASCR
Od běžných ke speciálním aplikacím zobrazovací hmotnostní spektrometrie
Bylo popsáno využití desorpčních ionizačních technik pro zobrazování distribuce molekul v typických i nestandardních aplikacích....
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