Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Farka, Z.; Hlaváček, Antonín; Mickert, M. J.; Skládal, P.; Gorris, H. H.
2018 - anglický
Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg·mL−1.
Klíčová slova:
photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without ...
Determination of permeability of ulra-fine lead oxide aerosols through military filters
Kellnerova, E.; Večeřa, Zbyněk; Kellner, J.; Zeman, T.; Hylak, Č.
2018 - anglický
Military filters introduced in the Army of the Czech Republic are most commonly used when working with combat equipment and weapons and in manoeuvring activities in the terrain with the use of explosives or chemical warfare agents. During such activities, a large number of pollutants in the form of nanoparticles are released. Nanoparticles, irrespective of their chemical composition, are classified as carcinogenic to humans and therefore it is necessary to eliminate them from the air. Military filters, especially the combined ones, which contain a filtration as well as sorption component, are most widely used and are designed for the widest range of pollutants. However, the current methodology to evaluate the effectiveness of military filters does not fix a duty to monitor the ability of the filters to capture relevant pollutants across the whole range of sizes. The paper evaluates the efficiency of selected types of military filters using the methodology and instrumentation introduced in the accredited laboratories of the Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic. The testing has been carried out simultaneously with two concentrations of ultra-fine aerosols containing lead oxide nanoparticles ranging in size from 7.6 nm to 299.6 nm. It is an aerosol, the physicochemical and toxicological properties of which are known. During the work the basic parameters of permeability of aerosols tested by filters have been evaluated, especially: size and number of particles in front of and behind the filter, the efficiency and penetration of nanoparticles by the filter.
Klíčová slova:
nanoparticle; filter; efficiency; health safety
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Determination of permeability of ulra-fine lead oxide aerosols through military filters
Military filters introduced in the Army of the Czech Republic are most commonly used when working with combat equipment and weapons and in manoeuvring activities in the terrain with the use of ...
Determination of Inorganic Arsenic in Samples of Marine Origin
Marschner, Karel; Matoušek, Tomáš; Dědina, Jiří; Musil, Stanislav
2017 - anglický
A fast screening method for determination of inorganic arsenic in marine samples was developed. This method is based on selective\nhydride generationanddetectionby inductively coupled plasmamass spectrometry. Using a high concentration of hydrochloric acid\ntogether with hydrogen peroxide for hydride generation leads to selective conversion of inorganic arsenic species into arsane. The\naccuracy of this method was verified by comparative analyse by high performance liquid chromatography coupled with hydride generationandatomic\nfluorescence detection.
Klíčová slova:
arsenic speciation; inorganic arsenic; hydride generation
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
Determination of Inorganic Arsenic in Samples of Marine Origin
A fast screening method for determination of inorganic arsenic in marine samples was developed. This method is based on selective\nhydride generationanddetectionby inductively coupled plasmamass ...
Screening of acyclovir permeation through skin using alaptide
Bobálová, Janette; Černíková, A.; Bobáľ, P.; Jampílek, J.
2016 - anglický
This investigation deals with the affection of the permeation of acyclovir through fullthickness\npig ear skin using a Franz diffusion cell from the donor vehicle of propylene\nglycol/water (1:1) using synthesised (S)-8-methyl-6,9-diazaspiro[4.5]decane-7,10-\ndione, alaptide as a potential transdermal permeation enhancer.
Klíčová slova:
acyclovir permeation; chemical permeation enhancers; skin permeation experiments
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
Screening of acyclovir permeation through skin using alaptide
This investigation deals with the affection of the permeation of acyclovir through fullthickness\npig ear skin using a Franz diffusion cell from the donor vehicle of propylene\nglycol/water (1:1) ...
Influence of inhaled manganese oxides nanoparticles on mass of internal organs in mice
Zeman, T.; Dočekal, Bohumil; Mikuška, Pavel; Buchtová, Marcela; Míšek, Ivan; Šerý, Omar; Večeřa, Zbyněk
2014 - anglický
Results of the statistical analysis of a long-term inhalation experiment on laboratory mice are presented. During 17 weeks of the experiment, the experimental group was exposed to inhaled manganese oxides nanoparticles. Manganese oxides (MnO center dot Mn2O3) nanoparticles (MnONPs) were synthesized continuously via aerosol route in a hot wall tube flow reactor using a thermal decomposition of metal organic precursor manganese(II) acetylacetonate in the flow tube reactor (in vertical position) at temperature 750 degrees C in the presence of 30 vol% of oxygen. The concentration of produced MnONPs at the reactor output was in the range 1-3 x 10(7) particles/cm(3) and the size of generated nanoparticles MnO center dot Mn2O3 was in the range 7-50 nm. Before entering the inhalation chamber, MnONPs in the mixture of N-2, O-2 and air (at total flow rate of 3 L/min) were further diluted using filtrated humidified air (20 L/min) at temperature 21 degrees C resulting in the MnONPs concentration 2 x 10(6) particles/cm(3). At regular time intervals the mass of selected internal organs of mice from both experimental and control group was assessed. It has been proven that inhaled nanoparticles are able to influence the mass of internal organs of mice. Statistically significantly lower mass of kidneys, liver and spleen and higher mass of pancreas have been found in the experimental group compared to the control group.
Klíčová slova:
inhaled nanoparticles; manganese oxides nanoparticles; changes in internal organ mass of mice; long-term inhalation experiment
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
Influence of inhaled manganese oxides nanoparticles on mass of internal organs in mice
Results of the statistical analysis of a long-term inhalation experiment on laboratory mice are presented. During 17 weeks of the experiment, the experimental group was exposed to inhaled manganese ...
Senzitive method of Caspase-3 detection in single stem cell
Adamová, Eva; Klepárník, Karel; Matalová, Eva
2013 - anglický
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Klíčová slova:
caspase-3; single stem cell; miniaturization
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
Senzitive method of Caspase-3 detection in single stem cell
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods ...
Separation of tryptic digest of cytochrome C within a long nanoelectrospray tip
Týčová, Anna; Foret, František
2013 - anglický
Capillary electrophoresis coupled to mass spectrometry (CE-MS) posses powerful tool for separation and detection of wide range of ionic species. To avoid complicated constructions of interfaces we have conducted the separation within a long and thin nanoelectrospray tip. We have investigated main properties of such an experimental design on a complex sample of tryptic digest of cytochrome c.
Klíčová slova:
cytochrome c; thin nanoelectrospray tip; electrophoretic separation
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
Separation of tryptic digest of cytochrome C within a long nanoelectrospray tip
Capillary electrophoresis coupled to mass spectrometry (CE-MS) posses powerful tool for separation and detection of wide range of ionic species. To avoid complicated constructions of interfaces we ...
New isoelectric focusing power supply based on features of voltage multiplier
Duša, Filip; Šlais, Karel
2013 - anglický
Present electrophoretic separation methods rely on sophisticated high-voltage power supplies capable of programing a voltage / current time course during an analysis. In this paper we suggest design of a simple highvoltage power supply for isoelectric focusing composed from affordable and commonly available electrical parts. It is based on features of the voltage multiplier invented by Cockcroft and Walton. Electrical characteristics of the power supply enabled power load controlled isoelectric focusing analysis thus eliminating need for programing voltage time course and reducing analysis total time.
Klíčová slova:
isoelectric focusing; IEF device; electrodes
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
New isoelectric focusing power supply based on features of voltage multiplier
Present electrophoretic separation methods rely on sophisticated high-voltage power supplies capable of programing a voltage / current time course during an analysis. In this paper we suggest ...
Thin metal films for detection and preconcentration
Podešva, Pavel; Foret, František
2013 - anglický
We have tested CE separation of selected samples in capillaries with polydopamine modified surface. The capillaries were modified by polydopamine or polydopamine with an additive. The polydopamine coating with additive represents a simple and effective procedure for capillary alteration by another modificator such as hydroxyethyl cellulose, hydroxypropyl cellulose etc. In this work, we represent separation data from PrinCE system with UV detection for peptides, proteins and oligosaccharides labeled by 2-aminobenzoic acid.
Klíčová slova:
thin metal film; chemoresistor; Au nanoparticles
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
Thin metal films for detection and preconcentration
We have tested CE separation of selected samples in capillaries with polydopamine modified surface. The capillaries were modified by polydopamine or polydopamine with an additive. The polydopamine ...
Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis
Vykydalová, Marie; Duša, Filip; Horká, Marie; Šlais, Karel
2013 - anglický
We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique.
Klíčová slova:
bidirectional isotachophoresis; electrolyte system; preparative focusing
Plné texty jsou dostupné na vyžádání prostřednictvím repozitáře Akademie věd.
Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis
We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on ...
NUŠL poskytuje centrální přístup k informacím o šedé literatuře vznikající v ČR v oblastech vědy, výzkumu a vzdělávání. Více informací o šedé literatuře a NUŠL najdete na webu služby.
Vaše náměty a připomínky posílejte na email nusl@techlib.cz
Provozovatel
Zahraniční báze