Počet nalezených dokumentů: 403
Publikováno od do

Structural analysis of polysaccharides from mushroom hericium Erinaceus mftccb 134
Bleha, R.; Čopíková, J.; Sushytskyi, Leonid; Vannucci, Luca; Sinica, A.; Jozífek, M.; Jablonský, I.; Klouček, P.
2023 - anglický
The aim of this research was the structural characterisation of polysaccharides isolated from fruiting bodies of the cultivating medicinal mushroom Hericium erinaceus MFTCCB 134. Cold and hot water extracts (CWE, HWE) were obtained and purified, and followed by alkali extraction from the reminder solids. The composition and structure of the fractions were analysed by FTIR spectroscopy, and neutral sugars/linkage analyses were determined by GC/FID and GC/MS, respectively. Galactose, fucose and glucose were found in purified CWE,\nwhile glucose predominated in purified HWE. Klíčová slova: Hericium erinaceus; polysaccharides; mushrooms Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Structural analysis of polysaccharides from mushroom hericium Erinaceus mftccb 134

The aim of this research was the structural characterisation of polysaccharides isolated from fruiting bodies of the cultivating medicinal mushroom Hericium erinaceus MFTCCB 134. Cold and hot water ...

Bleha, R.; Čopíková, J.; Sushytskyi, Leonid; Vannucci, Luca; Sinica, A.; Jozífek, M.; Jablonský, I.; Klouček, P.
Mikrobiologický ústav, 2023

Houby v jeskyních
Nováková, Alena
2021 - český
Práce zahrnuje údaje o zastoupení mikroskopických hub v různých evropských jeskyních (Česká republika, Slovensko, Rumunsko, Maďarsko, Slovinsko, Španělsko a Francie) získaný studiem v průběhu 20 let. Data about the occurrence of microscopic fungi in several European caves (Czech Republic, Slovakia, Romania, Hungary, Slovenia and Spain) obtained during 20 years studies are presented Klíčová slova: Microscopic fungi; cave environment Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Houby v jeskyních

Práce zahrnuje údaje o zastoupení mikroskopických hub v různých evropských jeskyních (Česká republika, Slovensko, Rumunsko, Maďarsko, Slovinsko, Španělsko a Francie) získaný studiem v průběhu 20 ...

Nováková, Alena
Mikrobiologický ústav, 2021

Enzymatic detoxification of cyanide using cyanide hydratases
Sedova, Anastasia; Rucká, Lenka; Glozlová, Michaela; Novotný, Petr; Martínková, Ludmila; Bojarová, Pavla
2021 - anglický
Cyanide, a strong chelator of metals in vital enzymes and proteins, is very toxic for most living organisms. Wastewaters from the mining, metallurgical and chemical industries contain significant concentrations of free cyanide. Though it can be largely eliminated by physicochemical methods, these processes may in turn lead to the formation of other waste. In addition, no effective methods have been found to neutralize cyanide spills coming from industrial accidents. The use of enzymes to remove cyanide is an attractive alternative, which is environmentally friendly and applicable to high cyanide concentrations. Cyanide hydratases (CynHs) are of considerable interest for the decomposition of hazardous cyanide wastes. Here we investigate the biochemical properties of new CynHs from Stereum hirsutum and Exidia glandulosa, it is of fundamental importance to preserve the enzyme activity at alkaline pH as cyanide waste is stored under these conditions. Klíčová slova: free cyanide; cyanide hydratase; cyanide spills; cyanide waste Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Enzymatic detoxification of cyanide using cyanide hydratases

Cyanide, a strong chelator of metals in vital enzymes and proteins, is very toxic for most living organisms. Wastewaters from the mining, metallurgical and chemical industries contain significant ...

Sedova, Anastasia; Rucká, Lenka; Glozlová, Michaela; Novotný, Petr; Martínková, Ludmila; Bojarová, Pavla
Mikrobiologický ústav, 2021

Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11.
Sushytskyi, L.; Lukáč, Pavol; Bleha, R.; Čopíková, J.; Vannucci, Luca; Sinica, A.; Kaštánek, P.
2019 - anglický
Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with AEC on DEAE Sepharose FF and SEC on Sepaphadex G-75 superfine columns. Two high MW (>1000 kDa and 98,3 kDa) polysaccharides (CPS1 and CPS3) were eluted with water and 0.5M NaCl on DEAE column, and heteropolysaccharide with MW of 15 kDa (CPS2) was also eluted with water on DEAE. The former fractions were identified as branched rhamnans, the latter one as branched xylogalactofuranan of unusual structure. CPS1 shown properties of unstable polimer: according to GPC analysis and by using light scattering and RI detectors it was found, that some fraction of polymers (260-800 kDa) have self-assembling properties in aqueous solution, and MW of this fraction decreased during some time while polimer with initially bigger MW (>1000 kDa) became bigger. CPS3 was eluted with 0.3-0.5 M of NaCl on DEAE Sepharose FF column and have MW of 98,3 kDa. Cell toxicity of the products were tested in vitro on cell cultures for different concentrations Klíčová slova: Mutant alga; Chlorella vulgaris G11; chromatography; polymer; cell cultures Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11.

Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with AEC on DEAE Sepharose FF and SEC on Sepaphadex G-75 superfine columns. Two high MW ...

Sushytskyi, L.; Lukáč, Pavol; Bleha, R.; Čopíková, J.; Vannucci, Luca; Sinica, A.; Kaštánek, P.
Mikrobiologický ústav, 2019

Název práce v českém jazyce\nVývin črevného mikrobiómu – jeho preventívna a terapeutická funkcia. \n
Kajaba, I.; Kajabová, A.; Genčíková, M.; Genčík, B.; Lovászová, N.; Bencko, V.; Turek, B.; Šíma, Petr; Ševčík, J.
2019 - český
V naší studii jsme zkoumali střevní mikrobiom, jeho preventivní a terapeutickou funkci In our study we examined the intestinal microbiome, its preventive and therapeutic function. Klíčová slova: Intestinal microbiome; immunity system; fecal transpalntation; dysbiosis Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Název práce v českém jazyce\nVývin črevného mikrobiómu – jeho preventívna a terapeutická funkcia. \n

V naší studii jsme zkoumali střevní mikrobiom, jeho preventivní a terapeutickou funkci


In our study we examined the intestinal microbiome, its preventive and therapeutic function.

Kajaba, I.; Kajabová, A.; Genčíková, M.; Genčík, B.; Lovászová, N.; Bencko, V.; Turek, B.; Šíma, Petr; Ševčík, J.
Mikrobiologický ústav, 2019

Význam skladby a aktivity mikrobiomu na homeostázu organismu (udržení zdraví)
Turek, B.; Šíma, Petr; Ševčík, J.; Kajaba, I.
2019 - český
Our study deals with the intestinal microbiome and its direct and indirect impact on overall health. Naše studie se zabývá střevním mikrobiomem a jeho přímým a nepřímým vlivem na celkové zdraví. Klíčová slova: Intestinal microbiome; microbes; immunity system Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Význam skladby a aktivity mikrobiomu na homeostázu organismu (udržení zdraví)

Our study deals with the intestinal microbiome and its direct and indirect impact on overall health.


Naše studie se zabývá střevním mikrobiomem a jeho přímým a nepřímým vlivem na celkové zdraví.

Turek, B.; Šíma, Petr; Ševčík, J.; Kajaba, I.
Mikrobiologický ústav, 2019

Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals
Neuhoferová, Eva; Petráková, V.; Vocetková, K.; Kindermann, Marek; Křivohlavá, Romana; Benson, Veronika
2018 - anglický
Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of therapeutic biomolecule into the wound without any undesired side effects. Given the fact that these non-healing wounds are result of impaired balance in metalloproteinases synthesized by immune cells residing the wounds, gene therapy offering knock down of such enzymes is of great interest. \nHere we challenged a development of functional and biocompatible wound dressing enabling controlled release of trackable carrier loaded with therapeutic siRNA. Our dressing consists of scaffold from degradable polymer nanofibers enriched with fluorescent nanodiamond particles (FND). We have previously shown the nanodiamond particles are great carriers for antisense RNAs. Their advantages represent high biocompatibility, stable luminescence giving us the possibility to track the carrier system in the wound, and effective release of antisense RNA in the wound. Embedding of nanodiamond-siRNA systems into nanofiber scaffold enables continuous release of siRNA and maintaining the stable siRNA concentration in the wound site resulting in a promotion of wound healing. \nWe developed FND-siRNA complexes specific to MMP-9 that efficiently inhibit the expression of target MMP-9 mRNA. The complexes were embedded into core/shell nanofibers from PVA and PCL, visualized by confocal microscopy, and characterized by electron microscopy. Real-time PCR was used to assess the silencing effect of siRNA that has been delivered to target murine fibroblasts by FND released from nanofiber dressing. Nanofiber system with embedded FNDs was applied on wounds in diabetic animal models to evaluate its suitability regarding short and long term toxicity, efficacy, and handling in vivo. \n Klíčová slova: Electrospun nanofibers; fluorescent nanodiamonds; RNA interference Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals

Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of ...

Neuhoferová, Eva; Petráková, V.; Vocetková, K.; Kindermann, Marek; Křivohlavá, Romana; Benson, Veronika
Mikrobiologický ústav, 2018

BETA-GLUCANS: WHAT APPLICATIONS? A GENERAL OVERVIEW
Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, Václav; Šíma, Petr
2018 - anglický
Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting cells. Otherwise, interest is rising for their use not only for modulation of immunity, but also of cholesterol levels, or as possible vaccine adjuvants, nanocarriers and functional food components. We will shortly review some aspects in human and veterinarian applications. Klíčová slova: Antitumor immune-responses; growth-performance; gastrointestinal-tract Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
BETA-GLUCANS: WHAT APPLICATIONS? A GENERAL OVERVIEW

Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting ...

Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, Václav; Šíma, Petr
Mikrobiologický ústav, 2018

BETA-GLUCANS: WHAT APPLICATIONS? A GENERAL OVERVIEW
Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, Václav; Šíma, Petr
2018 - anglický
Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting cells. Otherwise, interest is rising for their use not only for modulation of immunity, but also of cholesterol levels, or as possible vaccine adjuvants, nanocarriers and functional food components. We will shortly review some aspects in human and veterinarian applications. Klíčová slova: antitumor immune-responses; growth-performance; gastrointestinal-tract Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
BETA-GLUCANS: WHAT APPLICATIONS? A GENERAL OVERVIEW

Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting ...

Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, Václav; Šíma, Petr
Mikrobiologický ústav, 2018

Beta-Glucans: What Applications? A General Overview
Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, V.; Šíma, Petr
2018 - anglický
Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting cells. Otherwise, interest is rising for their use not only for modulation of immunity, but also of cholesterol levels, or as possible vaccine adjuvants, nanocarriers and functional food components. We will shortly review some aspects in human and veterinarian applications. Klíčová slova: antitumor immune-responses; growth-performance; gastrointestinal-tract Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Beta-Glucans: What Applications? A General Overview

Glucans are polysaccharides derived from various sources (e.g. mushrooms, algae, yeast, barley). They can target immune receptors, like Dectin-1 expressed on dendritic cells - antigen presenting ...

Vannucci, Luca; Mishra, Rajbardhan; Lukáč, Pavol; Tenti, Paolo; Rajsiglová, Lenka; Větvička, V.; Šíma, Petr
Mikrobiologický ústav, 2018

O službě

NUŠL poskytuje centrální přístup k informacím o šedé literatuře vznikající v ČR v oblastech vědy, výzkumu a vzdělávání. Více informací o šedé literatuře a NUŠL najdete na webu služby.

Vaše náměty a připomínky posílejte na email nusl@techlib.cz

Provozovatel

http://www.techlib.cz

Facebook

Zahraniční báze