No (118)
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Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Farka, Z.; Hlaváček, Antonín; Mickert, M. J.; Skládal, P.; Gorris, H. H.
2018 - English
Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg·mL−1.
Keywords:
photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac
Available at various institutes of the ASCR
Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without ...
Determination of permeability of ulra-fine lead oxide aerosols through military filters
Kellnerova, E.; Večeřa, Zbyněk; Kellner, J.; Zeman, T.; Hylak, Č.
2018 - English
Military filters introduced in the Army of the Czech Republic are most commonly used when working with combat equipment and weapons and in manoeuvring activities in the terrain with the use of explosives or chemical warfare agents. During such activities, a large number of pollutants in the form of nanoparticles are released. Nanoparticles, irrespective of their chemical composition, are classified as carcinogenic to humans and therefore it is necessary to eliminate them from the air. Military filters, especially the combined ones, which contain a filtration as well as sorption component, are most widely used and are designed for the widest range of pollutants. However, the current methodology to evaluate the effectiveness of military filters does not fix a duty to monitor the ability of the filters to capture relevant pollutants across the whole range of sizes. The paper evaluates the efficiency of selected types of military filters using the methodology and instrumentation introduced in the accredited laboratories of the Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic. The testing has been carried out simultaneously with two concentrations of ultra-fine aerosols containing lead oxide nanoparticles ranging in size from 7.6 nm to 299.6 nm. It is an aerosol, the physicochemical and toxicological properties of which are known. During the work the basic parameters of permeability of aerosols tested by filters have been evaluated, especially: size and number of particles in front of and behind the filter, the efficiency and penetration of nanoparticles by the filter.
Keywords:
nanoparticle; filter; efficiency; health safety
Available at various institutes of the ASCR
Determination of permeability of ulra-fine lead oxide aerosols through military filters
Military filters introduced in the Army of the Czech Republic are most commonly used when working with combat equipment and weapons and in manoeuvring activities in the terrain with the use of ...
SMV-2017-743: Preparation, testing and delivery of isoelectric point standards
Čmelík, Richard
2017 - English
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
Keywords:
Isoelectric focusing; pI markers; aminomethylphenols
Available on request at various institutes of the ASCR
SMV-2017-743: Preparation, testing and delivery of isoelectric point standards
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
SMV-2017-732: Preparation, testing and delivery of isoelectric point standards
Čmelík, Richard
2017 - English
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
Keywords:
Isoelectric focusing; pI markers; aminomethylphenols
Available on request at various institutes of the ASCR
SMV-2017-732: Preparation, testing and delivery of isoelectric point standards
The project continues long-term cooperation of both participants. The low-molecular mass pI markers were synthesized and examined with aim to fill up and deliver the previously described compounds.
Determination of Inorganic Arsenic in Samples of Marine Origin
Marschner, Karel; Matoušek, Tomáš; Dědina, Jiří; Musil, Stanislav
2017 - English
A fast screening method for determination of inorganic arsenic in marine samples was developed. This method is based on selective\nhydride generationanddetectionby inductively coupled plasmamass spectrometry. Using a high concentration of hydrochloric acid\ntogether with hydrogen peroxide for hydride generation leads to selective conversion of inorganic arsenic species into arsane. The\naccuracy of this method was verified by comparative analyse by high performance liquid chromatography coupled with hydride generationandatomic\nfluorescence detection.
Keywords:
arsenic speciation; inorganic arsenic; hydride generation
Available on request at various institutes of the ASCR
Determination of Inorganic Arsenic in Samples of Marine Origin
A fast screening method for determination of inorganic arsenic in marine samples was developed. This method is based on selective\nhydride generationanddetectionby inductively coupled plasmamass ...
Screening of acyclovir permeation through skin using alaptide
Bobálová, Janette; Černíková, A.; Bobáľ, P.; Jampílek, J.
2016 - English
This investigation deals with the affection of the permeation of acyclovir through fullthickness\npig ear skin using a Franz diffusion cell from the donor vehicle of propylene\nglycol/water (1:1) using synthesised (S)-8-methyl-6,9-diazaspiro[4.5]decane-7,10-\ndione, alaptide as a potential transdermal permeation enhancer.
Keywords:
acyclovir permeation; chemical permeation enhancers; skin permeation experiments
Available on request at various institutes of the ASCR
Screening of acyclovir permeation through skin using alaptide
This investigation deals with the affection of the permeation of acyclovir through fullthickness\npig ear skin using a Franz diffusion cell from the donor vehicle of propylene\nglycol/water (1:1) ...
SMV-2015-729: Preparation, testing and delivery of isoelectric point standards
Šlais, Karel
2015 - English
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously described aminomethylphenols. The prepared compounds are the good ampholytes with high hydrophility and molar absorbtivity.
Keywords:
Isoelectric focusing; low molecular mass pI markers; aminomethylphenols
Available on request at various institutes of the ASCR
SMV-2015-729: Preparation, testing and delivery of isoelectric point standards
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously ...
Influence of inhaled manganese oxides nanoparticles on mass of internal organs in mice
Zeman, T.; Dočekal, Bohumil; Mikuška, Pavel; Buchtová, Marcela; Míšek, Ivan; Šerý, Omar; Večeřa, Zbyněk
2014 - English
Results of the statistical analysis of a long-term inhalation experiment on laboratory mice are presented. During 17 weeks of the experiment, the experimental group was exposed to inhaled manganese oxides nanoparticles. Manganese oxides (MnO center dot Mn2O3) nanoparticles (MnONPs) were synthesized continuously via aerosol route in a hot wall tube flow reactor using a thermal decomposition of metal organic precursor manganese(II) acetylacetonate in the flow tube reactor (in vertical position) at temperature 750 degrees C in the presence of 30 vol% of oxygen. The concentration of produced MnONPs at the reactor output was in the range 1-3 x 10(7) particles/cm(3) and the size of generated nanoparticles MnO center dot Mn2O3 was in the range 7-50 nm. Before entering the inhalation chamber, MnONPs in the mixture of N-2, O-2 and air (at total flow rate of 3 L/min) were further diluted using filtrated humidified air (20 L/min) at temperature 21 degrees C resulting in the MnONPs concentration 2 x 10(6) particles/cm(3). At regular time intervals the mass of selected internal organs of mice from both experimental and control group was assessed. It has been proven that inhaled nanoparticles are able to influence the mass of internal organs of mice. Statistically significantly lower mass of kidneys, liver and spleen and higher mass of pancreas have been found in the experimental group compared to the control group.
Keywords:
inhaled nanoparticles; manganese oxides nanoparticles; changes in internal organ mass of mice; long-term inhalation experiment
Available on request at various institutes of the ASCR
Influence of inhaled manganese oxides nanoparticles on mass of internal organs in mice
Results of the statistical analysis of a long-term inhalation experiment on laboratory mice are presented. During 17 weeks of the experiment, the experimental group was exposed to inhaled manganese ...
SMV-2014-732: Preparation, testing and delivery of isoelectric point standards
Šlais, Karel
2014 - English
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously described aminomethylphenols. The prepared compounds are the good ampholytes with high hydrophility and molar absorbtivity.
Keywords:
Isoelectric focusing; low molecular mass pI markers; aminomethylphenols
Available on request at various institutes of the ASCR
SMV-2014-732: Preparation, testing and delivery of isoelectric point standards
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously ...
Senzitive method of Caspase-3 detection in single stem cell
Adamová, Eva; Klepárník, Karel; Matalová, Eva
2013 - English
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Keywords:
caspase-3; single stem cell; miniaturization
Available on request at various institutes of the ASCR
Senzitive method of Caspase-3 detection in single stem cell
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods ...
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