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Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2017 - English
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing.
Keywords:
enzyme production
Available at various institutes of the ASCR
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing.
Scale-up and production of various recombinant oxidoreductases
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2017 - English
Development and optimization of pilot cultivations and DSP of recombinant intracellular oxidoreductases in microbial productions for a food industrial use
Keywords:
enzyme production
Available at various institutes of the ASCR
Scale-up and production of various recombinant oxidoreductases
Development and optimization of pilot cultivations and DSP of recombinant intracellular oxidoreductases in microbial productions for a food industrial use
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
Kotrbáček, V.; Doucha, Jiří; Kuchařová, V.; Doubek, J.
2017 - English
We studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass significantly increased content of carotenoids in egg yolks of 8 experimental animals already after the first week of supplementation (P<0,01). Compared to 8 control hens these differences remained significantly higher until the end of the eight-week experiment. The average carotenoid concentration was 17,3 mu g per g of yolk in control group and 25,3 mu g per g of yolk in experimental animals (P<0,01). Autotrophically grown Chlorella was supplemented to the 30 gilts one month prior the expected first heat in a dose of 1,5 g per animal per day. These sows entered heat earlier and the number of pregnancy was higher in comparison to the 30 control animals. Other studies observed digestibility of selenium organically bound in Se-enriched Chlorella. The level of selenium in blood, colostrum and also tissues of new born piglets was determined. Group of 8 sows was supplemented with Se-enriched alga during the pregnancy and 3 days after parturition at a dose of 0,3 mg of selenium per 1 kg of feed. The same amount of selenium in the form of sodium selenite was administrated to the control group of 8 individuals. Organic form of selenium significantly increased concentration of Se in blood of sows and colostrum (P<0,05). Piglets born to supplemented sows had also significantly higher level of Se in heart, muscles and liver (P<0.01). The use of fresh Chlorella biomass produced outdoors on a dairy farm was experimentally tested. The liquid fresh biomass applied to dairy cows during the pregnancy at a dose of 15 g dry mass per cow increased the dry matter content in colostrum from 17,5 g per 100 ml of colostrum in control group to 20,5 g per 100 ml of colostrum in supplemented cows (P<0,05).
Keywords:
Chlorella; feed supplement; flue gas utilization
Available at various institutes of the ASCR
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
We studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass ...
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2017 - English
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up.
Keywords:
pastoris production
Available at various institutes of the ASCR
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up.
Development of the cultivation of the pathogenic bacteria.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2017 - English
Development of the cultivation of pathogenic bacteria with a specific lipopolysaccharide content in the cell structures. Optimization of media and yields.\n\n
Keywords:
cultivation of pathogens
Available at various institutes of the ASCR
Development of the cultivation of the pathogenic bacteria.
Development of the cultivation of pathogenic bacteria with a specific lipopolysaccharide content in the cell structures. Optimization of media and yields.\n\n
The Occurence Of Bla(Tem) And Tet(W) Genes In Czech Wastewaters And Their Fate In Fungal-Based Wastewater Treatment Processes
Svobodová, Kateřina; Adámek, M.; Petráčková, Denisa
2017 - English
Antibiotic resistant bacteria and genes (ARB&Gs) have presently emerged as serious environmental pollutants with a high health risk. This study analyzed the occurrence of ARGs tet(W), bla(TEM) and intl1 in microbiomes from 4 Czech wastewater treatment plants showing that bla(TEM) displayed slightly higher gene abundance compared to tet(W). Next, simulated wastewater seeded with activated sludge bacteria was treated with Pleurotus ostreatus-based bioreactor to evaluate the effect of the fungal filter on bacterial community structure and bacterial resistome in wastewaters. The results showed that functional diversity of bacterial communities was significantly changed by their contact with fungal biofilm as revealed by EcoPlate assays and analyses of bacterial 16S rDNA by denaturing gradient gel electrophoresis. A significant decrease in tet(W) abundance (48 times) was observed in fungal-treated wastewater compared to untreated one. The abundance of bla(TEM) genes increased 4 times during the adaptation of bacteria to nutrient conditions and remained unaffected by the fungal treatment. To our knowledge this work is first to study the fate of ARGs in wastewaters during their advanced biological treatment. It documented that P. ostreatus has a potential to lower the abundance of some ARGs in wastewaters.
Keywords:
Antibiotic resistant genes; wastewater treatment plant; Pleurotus ostreatus
Available at various institutes of the ASCR
The Occurence Of Bla(Tem) And Tet(W) Genes In Czech Wastewaters And Their Fate In Fungal-Based Wastewater Treatment Processes
Antibiotic resistant bacteria and genes (ARB&Gs) have presently emerged as serious environmental pollutants with a high health risk. This study analyzed the occurrence of ARGs tet(W), bla(TEM) and ...
Pilot scale production of a dietary supplement.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
Pilot scale cultivations of biotransformation enzymes producer, the biotransformation of the dietary supplement and its DSP.
Keywords:
dietary supplements; fungal fermentation
Available at various institutes of the ASCR
Pilot scale production of a dietary supplement.
Pilot scale cultivations of biotransformation enzymes producer, the biotransformation of the dietary supplement and its DSP.
Development of the anaerobic submerged cultivation.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
Development of the anaerobic cultivation of bacteria with a specific lipopolysaccharide content in the cell structures.
Keywords:
anaerobic cultivation
Available at various institutes of the ASCR
Development of the anaerobic submerged cultivation.
Development of the anaerobic cultivation of bacteria with a specific lipopolysaccharide content in the cell structures.
Development of pilot production technology of phosphorylases.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
Development and optimization of the production of phosporylases for pharmaceutical industry. Cultivation technology and down-stream processing.
Keywords:
enzyme production
Available at various institutes of the ASCR
Development of pilot production technology of phosphorylases.
Development and optimization of the production of phosporylases for pharmaceutical industry. Cultivation technology and down-stream processing.
Cultivation and DSP of the Pseudomonas bacteria to produce low molecular natural compounds.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
The set-up of the pilot cultivation of Pseudomonas spp. bacteria to produce specific low molecular natural compounds for pharmaceutical purposes.
Keywords:
natural substances production
Available at various institutes of the ASCR
Cultivation and DSP of the Pseudomonas bacteria to produce low molecular natural compounds.
The set-up of the pilot cultivation of Pseudomonas spp. bacteria to produce specific low molecular natural compounds for pharmaceutical purposes.
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