Number of found documents: 91
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Bacteriophage 936 by qPCR quantification in dairy products.
Olša Fliegerová, Kateřina; Mrázek, Jakub; Štrosová, Lenka; Němečková, I.; Kopečný, Jan
2018 - Czech
Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod of phage monitoring and quantification necessary part of phage prevention. Method is based on qPCR amplification and fluorescent detection of copies number of the phage main\nstructural protein and therefore is independent on the phage cultivation. It is quick, reliable a sensitive enough to estimate phage copy numbers in dairy starters and whey in any phase of dairy production. \n Virulentní bakteriofágy stále představují vážnou hrozbu pro průmyslové fermentace mléka, a to i při časté dezinfekci a sterilizaci všech zařízení v mlékárenských provozech. Proto je spolehlivá metoda monitorování přítomnosti bakteriofágů, ale zejména jejich kvantifikace, nutnou součástí boje proti virové infekci. Tato práce popisuje kvantifikaci laktokokového bakteriofága 936 pomocí polymerázové řetězové reakce (qPCR) a aplikaci tohoto postupu na vzorky provozních zákysů a syrovátek. Metoda je založena na amplifikaci a fluorescenční detekci nárůstu počtu kopií úseku genu pro hlavní strukturální protein fága 936 a je tudíž nezávislá na kultivaci bakteriofága. Metoda qPCR je rychlá, citlivá, umožňuje kvantifikaci bakteriofága 936 v malých objemech průmyslových vzorků a v kterékoliv fázi fermentačního procesu.\n Keywords: Bacteriophage 936; qPCR Available at various institutes of the ASCR
Bacteriophage 936 by qPCR quantification in dairy products.

Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod ...

Olša Fliegerová, Kateřina; Mrázek, Jakub; Štrosová, Lenka; Němečková, I.; Kopečný, Jan
Ústav živočišné fyziologie a genetiky, 2018

Sequencing of DNA variable fragment coding aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales \n
Killer, Jiří; Mrázek, Jakub
2018 - Czech
There was designed and tested a reliable marker for aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales. Method enables reliable a sensitive estimation in dairy starters and is independent on the phage cultivation. It is quick, reliable a sensitive estimation of dairy bacteria. It is possible to use it for basic research as well as for the effective analysis in dairy production plants. \n Byl připraven a ověřen marker (aspartyl-tRNA syntáza) pro klasifikaci a fylogenetické analýzy zástupců rodů náležejících do řádu Lactobacillales. Umožňuje rychlé a přesné druhové stanovení mléčných bakterií. Předkládanou metodiku lze využít v základním výzkumu při klasifikaci a fylogenetických analýzách vybraných rodů bakterií mléčného kvašení. Především je určen pro rychlé analýzy v provozech mléčného průmyslu. \n Keywords: Lactobacillales; dair bacteria Available at various institutes of the ASCR
Sequencing of DNA variable fragment coding aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus Lactobacillales \n

There was designed and tested a reliable marker for aspartyl-tRNA synthetase suitable for taxonomic classification and phylogenetic analysis of bacterial genera closely related to genus ...

Killer, Jiří; Mrázek, Jakub
Ústav živočišné fyziologie a genetiky, 2018

Establishing preclinical proof-of-concept of gene therapy for Huntington disease
Miniariková, J.; Juhás, Štefan; Caron, N.; Spronck, L.; Vallés, A.; De Haan, M.; Blits, B.; Ellederová, Zdeňka; van Deventer, S.; Petry, H.; Southwell, A.; Déglon, N.; Motlík, Jan; Konstantinová, P.; Evers, M.
2017 - English
Huntington disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the HTT gene. The translated expanded polyglutamine repeat in the huntingtin protein is known to cause toxic gain-of-function, affecting numerous cellular processes. Our approach involves a new therapeutic modality by developing a single (one-time) treatment for HD based on a gene therapy lowering the expression of the toxic huntingtin using the RNA interference (RNAi) mechanism. Huntingtin lowering is achieved using gene transfer of a cassette encoding an engineered microRNA targeting human HTT, delivered via adeno-associated viral vector serotype 5 (AAV5-miHTT). Keywords: Huntington´s disease Available at various institutes of the ASCR
Establishing preclinical proof-of-concept of gene therapy for Huntington disease

Huntington disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the HTT gene. The translated expanded polyglutamine repeat in the ...

Miniariková, J.; Juhás, Štefan; Caron, N.; Spronck, L.; Vallés, A.; De Haan, M.; Blits, B.; Ellederová, Zdeňka; van Deventer, S.; Petry, H.; Southwell, A.; Déglon, N.; Motlík, Jan; Konstantinová, P.; Evers, M.
Ústav živočišné fyziologie a genetiky, 2017

Oxidative stress in primary porcine fibroblasts expressing mutated huntingtin
Šmatlíková, Petra; Askeland, G.; Vaškovičová, Michaela; Klíma, Jiří; Motlík, Jan; Eide, L.; Ellederová, Zdeňka
2017 - English
Molecular events, such as protein aggregation, mitochondrial dysfunction, and transcriptional dysregulation have been linked to Huntington’s disease (HD) pathogenesis. Oxidative stress has been considered as one of the key players in disease progression. Though, it is still not clear whether oxidative stress causes HD, or if it is a consequence of other primary events. Keywords: huntingtin Available at various institutes of the ASCR
Oxidative stress in primary porcine fibroblasts expressing mutated huntingtin

Molecular events, such as protein aggregation, mitochondrial dysfunction, and transcriptional dysregulation have been linked to Huntington’s disease (HD) pathogenesis. Oxidative stress has been ...

Šmatlíková, Petra; Askeland, G.; Vaškovičová, Michaela; Klíma, Jiří; Motlík, Jan; Eide, L.; Ellederová, Zdeňka
Ústav živočišné fyziologie a genetiky, 2017

Results of the realisation of the experiments using large experimental animals – application of cells/ stem cells using different vectors, diagnostics
Juhás, Štefan; Juhásová, Jana
2017 - English
A design of methods to test application of cells/ stem cells using different vectors, solution of the application including diagnostics and connected agenda. Keywords: large animal models; stem cell therapy; spinal stem cells Available at various institutes of the ASCR
Results of the realisation of the experiments using large experimental animals – application of cells/ stem cells using different vectors, diagnostics

A design of methods to test application of cells/ stem cells using different vectors, solution of the application including diagnostics and connected agenda.

Juhás, Štefan; Juhásová, Jana
Ústav živočišné fyziologie a genetiky, 2017

In vivo IBD using minipig course results
Juhás, Štefan; Juhásová, Jana
2017 - Czech
Innovative approaches testing of Inflammatory Bowel Disease on minipigs. Testování inovativních postupů léčby IBD na miniaturních prasatech. Keywords: minipig; IBD; operational equipment Available at various institutes of the ASCR
In vivo IBD using minipig course results

Innovative approaches testing of Inflammatory Bowel Disease on minipigs.

Juhás, Štefan; Juhásová, Jana
Ústav živočišné fyziologie a genetiky, 2017

Results of the realisation of the experiments using the experimental animals
Juhás, Štefan; Juhásová, Jana
2017 - Czech
A design of methods to test a functionality of inovative replacements, solution of the replacement incl. implantation and connected agenda. Návrh způsobu ověření funkčnosti inovativních náhrad, řešení tohoto návrhu včetně implantací a souvisejících aktivit. Keywords: implants; experimental animals Available at various institutes of the ASCR
Results of the realisation of the experiments using the experimental animals

A design of methods to test a functionality of inovative replacements, solution of the replacement incl. implantation and connected agenda.

Juhás, Štefan; Juhásová, Jana
Ústav živočišné fyziologie a genetiky, 2017

Written report of the contractor on the results of the research in ophtalmology, implantation and cell biology.
Ardan, Taras
2017 - Czech
Research on the reaction of the organism and specific eye cells on the innovative implant developer by the contractor. Výzkum v oblasti reakce organismu a specifických buněk oka na inovativní implantát vyvinutý objednatelem. Keywords: intraocular; cells; implant Available at various institutes of the ASCR
Written report of the contractor on the results of the research in ophtalmology, implantation and cell biology.

Research on the reaction of the organism and specific eye cells on the innovative implant developer by the contractor.

Ardan, Taras
Ústav živočišné fyziologie a genetiky, 2017

Role of planar cell polarity pathway in ameloblastoma with focus on frizzled 6
Putnová, Barbora; Putnová, Iveta; Hrubá, Eva; Dosedělová, Hana; Štembírek, Jan; Daněk, Z.; Buchtová, Marcela
2017 - English
Ameloblastoma is the most common epithelial odontogenic tumour in the oral cavity. Its progression is usually slow and the surface is covered by an oral mucosa with physiologic appearance. These tumours do not cause any pain to the patient and therefore it is difficult to diagnose them on time and the first signs are often missed by clinician leading to late diagnosis. Keywords: ameloblastoma Available at various institutes of the ASCR
Role of planar cell polarity pathway in ameloblastoma with focus on frizzled 6

Ameloblastoma is the most common epithelial odontogenic tumour in the oral cavity. Its progression is usually slow and the surface is covered by an oral mucosa with physiologic appearance. These ...

Putnová, Barbora; Putnová, Iveta; Hrubá, Eva; Dosedělová, Hana; Štembírek, Jan; Daněk, Z.; Buchtová, Marcela
Ústav živočišné fyziologie a genetiky, 2017

Role of FGF signaling in zeugopod development
Buchtová, Marcela; Celá, Petra; Horáková, D.; Krejčí, P.
2017 - English
The fibroblast growth factor (FGF) signalling systém regulates many developmental processes including skeletogenesis, brain patterning, branching morphogenesis or limb development. as FGFs are key players in the variable processes of chondrogenesis, we experimentally manipulated this pathway to test its effect on zeugopode modeling. Keywords: FGF Available at various institutes of the ASCR
Role of FGF signaling in zeugopod development

The fibroblast growth factor (FGF) signalling systém regulates many developmental processes including skeletogenesis, brain patterning, branching morphogenesis or limb development. as FGFs are key ...

Buchtová, Marcela; Celá, Petra; Horáková, D.; Krejčí, P.
Ústav živočišné fyziologie a genetiky, 2017

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