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Beyond the Paywall: Strategic implications of a shifting ecosystem
Krueger, Stephanie
2019 - English
Keywords: meziknihovní výpůjčka; služby dodávání dokumentů; interlibrary loan; document delivery services; poplatky; knihovny; elektronické dodávání dokumentů; webové služby Available in the NTK library.
Beyond the Paywall: Strategic implications of a shifting ecosystem

Krueger, Stephanie
Národní technická knihovna, 2019

Aside from payment
Babaryka-Amelchanka, Veranika; Muravitskaya, Ryma; Shakura, Natallia
2019 - English
In order to provide users quality information in conjunction with the optimization of financial costs for information resources of the library, they use the opportunity of free acquisition and actively develop cooperation with other libraries and information centers in the field of document changing and resource sharing. In I.S. Lupinovich Belarus Agricultural Library of the National Academy of Sciences of Belarus there is functional system which includes, along with paid opportunities, mechanisms for free replenishment of its fund and mutual use of documents. Free acquisition of the fund is possible both thanks to acts regulated by the state and the National Academy of Sciences of Belarus, and through the development of partnerships with other libraries, organizations, and individuals through international exchange of documents, function of FAO depository, and receiving literature as a gift. The mutual use of resources is based on national and international interlibrary lending, participation in the World Network of Agricultural Libraries, and cooperation agreements with other libraries. The existing mechanisms contribute to ensuring access of Belarusian users to the world information resources on agriculture, as well as integration of the national sectoral information into the world information space with reduced financial costs. Keywords: AGLINET; Bělorusko; FAO depozitář; mezinárodní výměna dokumentů; akvizice otevřených zdrojů; free sources of acquisition; FAO depository; international document exchange; resource sharing; AGLINET; Belarus Available in the NTK library.
Aside from payment

In order to provide users quality information in conjunction with the optimization of financial costs for information resources of the library, they use the opportunity of free acquisition and ...

Babaryka-Amelchanka, Veranika; Muravitskaya, Ryma; Shakura, Natallia
Národní technická knihovna, 2019

Cross-border Cooperation in Czech-German Non-profit Organizations
KUTHANOVÁ, Kateřina
2019 - English
This thesis examines Czech-German cross-border cooperation within intercultural communication, identifies problems and develops possible solutions. The thesis is divided into a practical and a theoretical part. The theoretical part first describes the Czech-German cross-border cooperation and then intercultural communication, followed by a discussion of the context and the research methods. In the practical part, the author first performs a sequential analysis of data collected via interviews conducted with people working in the Tandem organization. The results of the analysis are interpreted, problems within the cooperation of Tandem are identified, and possible solutions developed. A glossary divided into two parts, recapitulating the definitions of relevant terms from the first two theoretical chapters of this thesis, is listed at the end of this work. A French résumé summarizing all the important points and conclusions is enclosed with the thesis. The aim of this thesis is to answer the following research question: "Linguistic and intercultural challenges of Czech-German non-profit cross-border cooperation: The case of the Tandem organization." This thesis examines Czech-German cross-border cooperation within intercultural communication, identifies problems and develops possible solutions. The thesis is divided into a practical and a theoretical part. The theoretical part first describes the Czech-German cross-border cooperation and then intercultural communication, followed by a discussion of the context and the research methods. In the practical part, the author first performs a sequential analysis of data collected via interviews conducted with people working in the Tandem organization. The results of the analysis are interpreted, problems within the cooperation of Tandem are identified, and possible solutions developed. A glossary divided into two parts, recapitulating the definitions of relevant terms from the first two theoretical chapters of this thesis, is listed at the end of this work. A French résumé summarizing all the important points and conclusions is enclosed with the thesis. The aim of this thesis is to answer the following research question: "Linguistic and intercultural challenges of Czech-German non-profit cross-border cooperation: The case of the Tandem organization." Keywords: Cross-border cooperation; non-profit organizations; intercultural communication Available in the Digital Repository of University of South Bohemia.
Cross-border Cooperation in Czech-German Non-profit Organizations

This thesis examines Czech-German cross-border cooperation within intercultural communication, identifies problems and develops possible solutions. The thesis is divided into a practical and a ...

KUTHANOVÁ, Kateřina
Jihočeská univerzita v Českých Budějovicích, 2019

Testing cross-amplification of microsatellites on European Ranunculus sect. Batrachium species
GEMEINHARDT, Helene Sabine
2019 - English
Cross-amplification of microsatellites on European Ranunculus sect. Batrachium species was tested using primers that were designed for R. bungei. The main aim was to test whether these primers anneal successfully and the regions can be amplified using PCR in order to use them for population genetical studies. Cross-amplification of microsatellites on European Ranunculus sect. Batrachium species was tested using primers that were designed for R. bungei. The main aim was to test whether these primers anneal successfully and the regions can be amplified using PCR in order to use them for population genetical studies. Available in the Digital Repository of University of South Bohemia.
Testing cross-amplification of microsatellites on European Ranunculus sect. Batrachium species

Cross-amplification of microsatellites on European Ranunculus sect. Batrachium species was tested using primers that were designed for R. bungei. The main aim was to test whether these primers anneal ...

GEMEINHARDT, Helene Sabine
Jihočeská univerzita v Českých Budějovicích, 2019

The interaction of Streptomyces-like bacteria and model microorganisms in secondary metabolite production, motility and hemolytic activities - Experimental
WURZER, Theresa
2019 - English
In this thesis, environmental and clinical Streptomyces strains were used to test the hypothesis if isolates from different isolation sources vary in frequency of beta-hemolytic activity. Moreover, their interaction with two human pathogens Pseudomonas aeruginosa and Staphylococcus aureus was tested in parallel and T-type co-cultivations. For this, the responses (beta-hemolytic activity, growth, etc.) of both streptomycetes as well as model pathogens were evaluated. Furthermore, the frequency of observed interaction patterns with tested pathogens were compared between environmental and clinical Streptomyces strains as such study is lacking. Keywords: Streptomycetes; microbial interactions; secondary metabolites; hemolysis Available in the Digital Repository of University of South Bohemia.
The interaction of Streptomyces-like bacteria and model microorganisms in secondary metabolite production, motility and hemolytic activities - Experimental

In this thesis, environmental and clinical Streptomyces strains were used to test the hypothesis if isolates from different isolation sources vary in frequency of beta-hemolytic activity. Moreover, ...

WURZER, Theresa
Jihočeská univerzita v Českých Budějovicích, 2019

Expression and purification of recombinant Sericin-2 and Salivary gland secretion 3
VU, Trang Thanh
2019 - English
Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and analyzed using different molecular biology techniques. The pure proteins were tested for cell adhesive properties by coating hydrophobic surfaces with the proteins and monitoring the growth of adherent cells in vitro. By showing adequate expression and resultant yield, as well as the ability to aid cell adhesion, the adhesive proteins present great potential for future biomaterials. Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and analyzed using different molecular biology techniques. The pure proteins were tested for cell adhesive properties by coating hydrophobic surfaces with the proteins and monitoring the growth of adherent cells in vitro. By showing adequate expression and resultant yield, as well as the ability to aid cell adhesion, the adhesive proteins present great potential for future biomaterials. Keywords: proteins; recombinant; expression; bombyx; drosophila Available in the Digital Repository of University of South Bohemia.
Expression and purification of recombinant Sericin-2 and Salivary gland secretion 3

Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and analyzed using different molecular biology ...

VU, Trang Thanh
Jihočeská univerzita v Českých Budějovicích, 2019

Functional characterization of succinyl-CoA synthetase in the bloodstream form of Trypanosoma brucei
KUBIŠOVÁ, Karolína
2019 - English
During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse activity of the FoF1-ATP synthase to hydrolyze ATP and pump protons into the mitochondrial intermembrane space to maintain the essential membrane potential. The dogma also states that the sole source of organellar ATP is the mitochondrial ADP/ATP carrier, which imports glycolytically derived ATP from the cytosol. In this study, we explore the possibility that the BF mitochondrion is able to contribute to its own pool of consumable ATP by utilizing an active succinyl-CoA synthase to generate ATP via substrate phosphorylation. During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse activity of the FoF1-ATP synthase to hydrolyze ATP and pump protons into the mitochondrial intermembrane space to maintain the essential membrane potential. The dogma also states that the sole source of organellar ATP is the mitochondrial ADP/ATP carrier, which imports glycolytically derived ATP from the cytosol. In this study, we explore the possibility that the BF mitochondrion is able to contribute to its own pool of consumable ATP by utilizing an active succinyl-CoA synthase to generate ATP via substrate phosphorylation. Available in the Digital Repository of University of South Bohemia.
Functional characterization of succinyl-CoA synthetase in the bloodstream form of Trypanosoma brucei

During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse ...

KUBIŠOVÁ, Karolína
Jihočeská univerzita v Českých Budějovicích, 2019

Trehalose as an important energy source for immune cells in Drosophila melanogaster
LEHR, Katharina
2019 - English
The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by Leptopilina boulardi. Moreover, the energy supply was shut down by deactivating the trehalose transporter and measuring the lamellocyte production, again in uninfected and infected larva to test the role of trehalose for the selfish immune system. The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by Leptopilina boulardi. Moreover, the energy supply was shut down by deactivating the trehalose transporter and measuring the lamellocyte production, again in uninfected and infected larva to test the role of trehalose for the selfish immune system. Available in the Digital Repository of University of South Bohemia.
Trehalose as an important energy source for immune cells in Drosophila melanogaster

The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by ...

LEHR, Katharina
Jihočeská univerzita v Českých Budějovicích, 2019

Genome analysis of Kutzneria sp. strain BCCO 10_1627 and detection of antifungal secondary metabolites
JERNEJ, Linda
2019 - English
Secondary metabolites, produced by various microbes, are used since decades for treatment of diseases. The bacterial genus Kutzneria is known for synthesizing compounds with antifungal activity, which might be used against pathogenic fungi, causing agricultural relevant diseases. This thesis deals with the genome analysis of the strain Kutzneria sp. BCCO 10_1627 for prediction of secondary metabolite gene cluster. Moreover, the antifungal activity of the strain was determined experimentally. Secondary metabolites, produced by various microbes, are used since decades for treatment of diseases. The bacterial genus Kutzneria is known for synthesizing compounds with antifungal activity, which might be used against pathogenic fungi, causing agricultural relevant diseases. This thesis deals with the genome analysis of the strain Kutzneria sp. BCCO 10_1627 for prediction of secondary metabolite gene cluster. Moreover, the antifungal activity of the strain was determined experimentally. Available in the Digital Repository of University of South Bohemia.
Genome analysis of Kutzneria sp. strain BCCO 10_1627 and detection of antifungal secondary metabolites

Secondary metabolites, produced by various microbes, are used since decades for treatment of diseases. The bacterial genus Kutzneria is known for synthesizing compounds with antifungal activity, which ...

JERNEJ, Linda
Jihočeská univerzita v Českých Budějovicích, 2019

Characterization of the recombinant proteins SER 3 and BARN
KARL, Lorenz
2019 - English
This thesis shows the process of expressing two promising recombinant proteins in a bacterial expression system and testing the adhesive properties of the pure properties in a cell culture experiment. Moreover, this thesis presents an overview of several application possibilities for bioadhesive materials. This thesis shows the process of expressing two promising recombinant proteins in a bacterial expression system and testing the adhesive properties of the pure properties in a cell culture experiment. Moreover, this thesis presents an overview of several application possibilities for bioadhesive materials. Available in the Digital Repository of University of South Bohemia.
Characterization of the recombinant proteins SER 3 and BARN

This thesis shows the process of expressing two promising recombinant proteins in a bacterial expression system and testing the adhesive properties of the pure properties in a cell culture ...

KARL, Lorenz
Jihočeská univerzita v Českých Budějovicích, 2019

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