Number of found documents: 443
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Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans
Partyka, Jan; Křenková, Jana; Čmelík, Richard; Foret, František
2018 - English
Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number of positive charges allowed to use effective and sensitive CE-MS analysis in positive ion mode both neutral and acidic glycans. Keywords: CE-MS analysis; oligosaccharides; amino acids; peptides Fulltext is available at external website.
Hexahistidine-based tag for capillary electrophoresis-mass spektrometry analysis of oligosaccharides and N-linked glycans

Various basic amino acids and peptides were studied as an alternative labels for capillary electrophoresis-mass spectrometry analysis of oligosaccharides. The hexahistidine-based label with big number ...

Partyka, Jan; Křenková, Jana; Čmelík, Richard; Foret, František
Ústav analytické chemie, 2018

Supercritical water in etching and morphing of siliceous surfaces for analytical separations
Karásek, Pavel; Horká, Marie; Planeta, Josef; Roth, Michal
2018 - English
Applications of supercritical water in etching and morphing of siliceous surfaces for analytical separations are briefly reviewed. Keywords: supercritical water; fused silica capillary; electromigration methods Available in digital repository of the ASCR
Supercritical water in etching and morphing of siliceous surfaces for analytical separations

Applications of supercritical water in etching and morphing of siliceous surfaces for analytical separations are briefly reviewed.

Karásek, Pavel; Horká, Marie; Planeta, Josef; Roth, Michal
Ústav analytické chemie, 2018

Capillary electrophoresis in clinical diagnosis and monitoring
Kubáň, Petr
2018 - English
There is a significant potential of CE to provide a useful analytical tool to analyze noninvasive samples of body fluids and stimulate the cooperation between analytical chemists and clinicians in this interdisciplinary field. Keywords: CE; CE-LIF; clinical diagnosis Available in digital repository of the ASCR
Capillary electrophoresis in clinical diagnosis and monitoring

There is a significant potential of CE to provide a useful analytical tool to analyze noninvasive samples of body fluids and stimulate the cooperation between analytical chemists and clinicians in ...

Kubáň, Petr
Ústav analytické chemie, 2018

CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis
Foret, František; Křenková, Jana; Drobníková, Iveta; Klepárník, Karel; Přikryl, Jan
2018 - English
This year the meeting included full texts of 25 oral and 61 poster presentations. The attached conference proceedings, edited by highly cited scientists, documents the quality of the meeting. Similarly to the previous years the lectures were given by outstanding, highly cited, scientists. The list of speakers as well as more detailed information about the conference can be found on its permanent web at www.ce-ce.org. Keywords: bioanalysis; separation; biotechnology Fulltext is available at external website.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis

This year the meeting included full texts of 25 oral and 61 poster presentations. The attached conference proceedings, edited by highly cited scientists, documents the quality of the meeting. ...

Foret, František; Křenková, Jana; Drobníková, Iveta; Klepárník, Karel; Přikryl, Jan
Ústav analytické chemie, 2018

Surface enhanced raman spectroscopy: Detection platforms for sample analysis with and without electrophoretic separation
Týčová, Anna; Přikryl, Jan; Foret, František; Belder, D.
2018 - English
In this work we present two platforms of relatively simple construction for user-friendly sample detection via surface enhanced Raman spectroscopy (SERS). The first platform is designed for direct sample analysis and deals with hydrophilic spots array within a hydrophobic surface. The second presented platform aims on analysis of samples of higher complexity requiring separation step prior the detection. This is achived via microfluidic electrophoretic device with online SERS detection. Keywords: raman spectroscopy; SERS; microfluidic electrophoretic device Fulltext is available at external website.
Surface enhanced raman spectroscopy: Detection platforms for sample analysis with and without electrophoretic separation

In this work we present two platforms of relatively simple construction for user-friendly sample detection via surface enhanced Raman spectroscopy (SERS). The first platform is designed for direct ...

Týčová, Anna; Přikryl, Jan; Foret, František; Belder, D.
Ústav analytické chemie, 2018

Epitachophoretic separation and concentration of large volume samples
Voráčová, Ivona; Foret, František; Datinská, Vladimíra; Novotný, Jakub; Gheibi, P.; Berka, J.; Astier, Y.
2018 - English
There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also concentration of DNA from large volume samples is required. We have developed a new device suitable for separation, concentration and collection of ions from several milliliter sample volumes into microliter fractions. We have used a discontinuous electrolyte system comprising of the leading and terminating electrolytes utilized for concentration of samples containing DNA fragments. The method is simple, fast with high concentration factor and without extensive surface interactions. Keywords: epitachophoretic separation; DNA; large sample volumes Fulltext is available at external website.
Epitachophoretic separation and concentration of large volume samples

There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also ...

Voráčová, Ivona; Foret, František; Datinská, Vladimíra; Novotný, Jakub; Gheibi, P.; Berka, J.; Astier, Y.
Ústav analytické chemie, 2018

Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Dvořák, Miloš; Miková, B.; Šlampová, Andrea; Kubáň, Pavel
2018 - English
Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM NaOH solution, which acted as the HF-LPME donor solution, and simultaneously the neutralized drugs were transferred across the HF and preconcentrated in 10 mM HCl acceptor solution inside the HF lumen. Mixture (1:1, v:v) of 1-ethyl-2-nitrobenzene and dihexyl ether was used for impregnation of the porous HF membrane. Analytes in the acceptor solution were injected directly from the HF into capillary electrophoresis instrument for further separation, detection and quantification. Repeatability of the hyphenated analytical system ranged from 9.8 to 13.8 % (RSD) and enrichment factors of 52 – 78 were obtained for model basic drugs spiked to DBS extracts at a concentration level of 0.1 mg/L. Keywords: hollow fibre-liquid phase microextraction; microextraction of basic drugs; dried blood spots Fulltext is available at external website.
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots

Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM ...

Dvořák, Miloš; Miková, B.; Šlampová, Andrea; Kubáň, Pavel
Ústav analytické chemie, 2018

Vliv inhalovaných nanočástic oxidů kovů na orgány exponovaných myší
Mikuška, Pavel; Večeřa, Zbyněk; Dočekal, Bohumil; Čapka, Lukáš; Křůmal, Kamil; Coufalík, Pavel; Moravec, Pavel; Buchtová, Marcela; Míšek, Ivan; Vrlíková, Lucie; Smutná, Tereza; Dumková, J.; Hampl, A.; Fictum, P.
2018 - Czech
Dospělé myši byly kontinuálně vystaveny nanočásticím oxidů kovů v různých časových intervalech. Na konci experimentu byly orgány myší (plíce, mozek, ledviny, játra a slezina) analyzovány chemickými, biochemickými, histologickými a elektron mikroskopickými analýzami. Adult mice were continuously exposed to metal oxide nanoparticles for different time intervals. At the end of experiment, mice organs (lung, brain, kidney, liver and spleen) were collected for chemical, biochemical, histological and electron microscopical analyses. Keywords: nanoparticles; inhalation; mice Available in digital repository of the ASCR
Vliv inhalovaných nanočástic oxidů kovů na orgány exponovaných myší

Dospělé myši byly kontinuálně vystaveny nanočásticím oxidů kovů v různých časových intervalech. Na konci experimentu byly orgány myší (plíce, mozek, ledviny, játra a slezina) analyzovány chemickými, ...

Mikuška, Pavel; Večeřa, Zbyněk; Dočekal, Bohumil; Čapka, Lukáš; Křůmal, Kamil; Coufalík, Pavel; Moravec, Pavel; Buchtová, Marcela; Míšek, Ivan; Vrlíková, Lucie; Smutná, Tereza; Dumková, J.; Hampl, A.; Fictum, P.
Ústav analytické chemie, 2018

Složení prachových částic v emisích ze spalování uhlí v různých typech kotlů používaných pro vytápění domácností
Křůmal, Kamil; Mikuška, Pavel; Horák, J.; Hopan, F.
2018 - Czech
Organické sloučeniny (polycyklické aromatické uhlovodíky, alkany) a organické markery (hopany) používané pro identifikaci emisních zdrojů aerosolů byly měřeny v emisích ze spalování černého a hnědého uhlí. Uhlí bylo spalováno v různých typech kotlů (prohořívací, odhořívací, automatický kotel) používaných pro vytápění domácností v České republice. Organic compounds (polycyclic aromatic hydrocarbons, alkanes) and organic markers (hopanes) used for the identification of aerosol emission sources were measured in emissions from the combustion of hard and brown coal. Coal was combusted in different boilers (overfire boiler, boiler with down-draft combustion and automatic boiler) used for residential heating in the Czech Republic. Keywords: residential heating; household heating; coal combustion Available at various institutes of the ASCR
Složení prachových částic v emisích ze spalování uhlí v různých typech kotlů používaných pro vytápění domácností

Organické sloučeniny (polycyklické aromatické uhlovodíky, alkany) a organické markery (hopany) používané pro identifikaci emisních zdrojů aerosolů byly měřeny v emisích ze spalování černého a hnědého ...

Křůmal, Kamil; Mikuška, Pavel; Horák, J.; Hopan, F.
Ústav analytické chemie, 2018

Photon-upconverting nanoparticles as a novel background-free label in immunoassays
Farka, Z.; Hlaváček, Antonín; Mickert, M. J.; Skládal, P.; Gorris, H. H.
2018 - English
Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg·mL−1. Keywords: photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac Available at various institutes of the ASCR
Photon-upconverting nanoparticles as a novel background-free label in immunoassays

Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without ...

Farka, Z.; Hlaváček, Antonín; Mickert, M. J.; Skládal, P.; Gorris, H. H.
Ústav analytické chemie, 2018

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