No (143)
Yes (1)
Number of found documents:
144
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Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Martínková, Ludmila; Kaplan, Ondřej; Veselá, Alicja Barbara
2014 - English
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. The enzymes differ from each other in substrate specificity, temperature optimum, enantioselectivity (arylacetonitrilases) etc. Preferential substrates are phenylacetonitrile, (R,S)-mandelonitrile, benzonitrile or cyanopyridines (depending on the enzyme).
Keywords:
Nitrilase; Expression; Purification
Available at various institutes of the ASCR
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. ...
Vývoj technologií lyopfilizace buněčných lyzátů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Keywords:
lyophyllization; cell content; biotechnology
Available at various institutes of the ASCR
Vývoj technologií lyopfilizace buněčných lyzátů
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Brennerová, Mária
2014 - English
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria from technological solutions, and taxonomical identification through sequencing of 16S ribosomal RNA genes and searching for affiliations with bacterial relatives in the NCBI website.
Keywords:
viscose; technological solutions; bacterial isolates; identification
Available at various institutes of the ASCR
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria ...
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Keywords:
enzyme production; bacterial production; submergeed aerobic cultivation
Available at various institutes of the ASCR
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
Kozáková, Hana
2014 - English
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete microflora.
Keywords:
flies; lactobacili; mice
Available at various institutes of the ASCR
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete ...
Transfer of technology for production of AEH from Sphingomonas sp
Kyslíková, Eva; Palyzová, Andrea; Marešová, Helena; Štěpánek, Václav; Zahradník, Jiří; Bečka, Stanislav; Dobišová, Marie; Kyslík, Pavel
2014 - English
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross linked enzyme agregate
Keywords:
Alpha aminoacid ester hydrolase; heterologous expression; stirred bioreactor
Available at various institutes of the ASCR
Transfer of technology for production of AEH from Sphingomonas sp
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross ...
Hydrodynamic Study of the Downward Annular Gas-Liquid Flow under the Pilot Plant Operating Conditions 2013-2014.
Stavárek, Petr; Křišťál, Jiří
2014 - English
Hydrodynamics of annular liquid flow in a vertical tube with cocurrent gas flow has been studied. Effect of gas - liquid flow rate and liquid viscosity on two-phase flow hydrodynamics was investigated for glycerol – air system. The evaluated parameters were pressure drop and hydrodynamic regime at different gas/liquid ratios.
Keywords:
annular gas-liquid flow; pressure drop; hydrodynamics
Available at various institutes of the ASCR
Hydrodynamic Study of the Downward Annular Gas-Liquid Flow under the Pilot Plant Operating Conditions 2013-2014.
Hydrodynamics of annular liquid flow in a vertical tube with cocurrent gas flow has been studied. Effect of gas - liquid flow rate and liquid viscosity on two-phase flow hydrodynamics was investigated ...
New inorganic matrices for pultrusion technology
Černý, Zbyněk
2014 - English
Development of new inorganic matrix enabling rapid continuous preparation of thermally and chemically stable fire resistant technological profiles and components with controlled properties.
Keywords:
pultrusion technology; inorganic matrice
Available at various institutes of the ASCR
New inorganic matrices for pultrusion technology
Development of new inorganic matrix enabling rapid continuous preparation of thermally and chemically stable fire resistant technological profiles and components with controlled properties.
18M-IMMEDIATE Research Report: Prototype of Catalyst Powder Sufficient for Testing in MEAs Fabricated
Kaluža, Luděk; Zdražil, Miroslav; Gulková, Daniela; Koštejn, Martin; Vít, Zdeněk
2014 - English
High loadings of platinum (60 wt%) were precipitated (ICPF) or depo-sited (CNRS) onto research grade carbon black supports provided by TC. The supports and the resulting electrocatalysts were thoroughly characterised in order to select the best ones for optimisation and up-scaled manufacturing. For the preparation and characterisation vari-ous methodologies have been developed. Batches of the gram size were prepared of a handful of the most promising catalysts. This is sufficient for the preparation of test MEAs.
Keywords:
Pt catalysts; stress tests; nitrogen physisorption
Available at various institutes of the ASCR
18M-IMMEDIATE Research Report: Prototype of Catalyst Powder Sufficient for Testing in MEAs Fabricated
High loadings of platinum (60 wt%) were precipitated (ICPF) or depo-sited (CNRS) onto research grade carbon black supports provided by TC. The supports and the resulting electrocatalysts were ...
SMV-2014-732: Preparation, testing and delivery of isoelectric point standards
Šlais, Karel
2014 - English
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously described aminomethylphenols. The prepared compounds are the good ampholytes with high hydrophility and molar absorbtivity.
Keywords:
Isoelectric focusing; low molecular mass pI markers; aminomethylphenols
Available on request at various institutes of the ASCR
SMV-2014-732: Preparation, testing and delivery of isoelectric point standards
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously ...
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