Number of found documents: 51
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Examination of the Spatial Structure of Pigs' Melanoma in Tissue Sections Based on Histology and Mass Spectrometry
Anýz, J.; Vysloužilová, L.; Horák, Vratislav; Štěpánková, O.; Vaculovič, T.; Adam, V.
2019 - English
We examine the spatial structure of the melanoma in tissue sections. The pigs melanoma was examined in 10 tissue samples obtained from animals of age between 4 and 22 weeks. The tissue sections were measured by light microscopy and Laser Ablation Ion Inductively Coupled Plasma Mass Spectrometry to obtain spatial metal (Cu, Zn) distribution. The exploratory analysis of the tissue sections indicates there is clearly a pattern in the spatial structure. Different projections of the spatial structure of the melanoma are obtained by the different measurement methods. The spectral clustering on the data was utilized to describe the structure in the data. According to the clustering results, there are distinct clusters of observations in the histological data. The spatial elemental distribution of the metals Cu and Zn cannot be clustered -the data form one compact cluster. The clustering of the histological images produces clusters which are related to the annotation of the biological samples in broader terms -the differences between fibrous and cancerous tissue. Keywords: MeLiM; melanoma Available at various institutes of the ASCR
Examination of the Spatial Structure of Pigs' Melanoma in Tissue Sections Based on Histology and Mass Spectrometry

We examine the spatial structure of the melanoma in tissue sections. The pigs melanoma was examined in 10 tissue samples obtained from animals of age between 4 and 22 weeks. The tissue sections were ...

Anýz, J.; Vysloužilová, L.; Horák, Vratislav; Štěpánková, O.; Vaculovič, T.; Adam, V.
Ústav živočišné fyziologie a genetiky, 2019

Phenotypic Analyses of the HD Transgenic Minipig Model (A11609)
Ellederová, Zdeňka
2018 - English
The transgenic Huntington's disease minipigs (TgHD) express N‐terminal part of human mutated huntingtin (124Q) under the control of human huntingtin promoter. The founder animal, born in 2009, gave birth to four subsequent generations with an equal contribution of wild‐type (WT) and transgenic (TgHD) piglets in all litters. The model is being used for preclinical huntingtin lowering studies. Here we take different non-invasive and invasive approaches, some of which are unique for large animal models, to study the phenotype development comparing WT and TgHD siblings. We show gradual progression of the disease in these TgHD animals. Moreover, some biomarkers were identified. These markers could serve for monitoring of organism response to HD treatment to assess efficacy and safety in preclinical studies prior to human clinical trials. Keywords: minipig Available at various institutes of the ASCR
Phenotypic Analyses of the HD Transgenic Minipig Model (A11609)

The transgenic Huntington's disease minipigs (TgHD) express N‐terminal part of human mutated huntingtin (124Q) under the control of human huntingtin promoter. The founder animal, born in 2009, gave ...

Ellederová, Zdeňka
Ústav živočišné fyziologie a genetiky, 2018

Role of planar cell polarity pathway in ameloblastoma with focus on frizzled 6
Putnová, Barbora; Putnová, Iveta; Hrubá, Eva; Dosedělová, Hana; Štembírek, Jan; Daněk, Z.; Buchtová, Marcela
2017 - English
Ameloblastoma is the most common epithelial odontogenic tumour in the oral cavity. Its progression is usually slow and the surface is covered by an oral mucosa with physiologic appearance. These tumours do not cause any pain to the patient and therefore it is difficult to diagnose them on time and the first signs are often missed by clinician leading to late diagnosis. Keywords: ameloblastoma Available at various institutes of the ASCR
Role of planar cell polarity pathway in ameloblastoma with focus on frizzled 6

Ameloblastoma is the most common epithelial odontogenic tumour in the oral cavity. Its progression is usually slow and the surface is covered by an oral mucosa with physiologic appearance. These ...

Putnová, Barbora; Putnová, Iveta; Hrubá, Eva; Dosedělová, Hana; Štembírek, Jan; Daněk, Z.; Buchtová, Marcela
Ústav živočišné fyziologie a genetiky, 2017

Establishing preclinical proof-of-concept of gene therapy for Huntington disease
Miniariková, J.; Juhás, Štefan; Caron, N.; Spronck, L.; Vallés, A.; De Haan, M.; Blits, B.; Ellederová, Zdeňka; van Deventer, S.; Petry, H.; Southwell, A.; Déglon, N.; Motlík, Jan; Konstantinová, P.; Evers, M.
2017 - English
Huntington disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the HTT gene. The translated expanded polyglutamine repeat in the huntingtin protein is known to cause toxic gain-of-function, affecting numerous cellular processes. Our approach involves a new therapeutic modality by developing a single (one-time) treatment for HD based on a gene therapy lowering the expression of the toxic huntingtin using the RNA interference (RNAi) mechanism. Huntingtin lowering is achieved using gene transfer of a cassette encoding an engineered microRNA targeting human HTT, delivered via adeno-associated viral vector serotype 5 (AAV5-miHTT). Keywords: Huntington´s disease Available at various institutes of the ASCR
Establishing preclinical proof-of-concept of gene therapy for Huntington disease

Huntington disease (HD) is an autosomal dominant neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the HTT gene. The translated expanded polyglutamine repeat in the ...

Miniariková, J.; Juhás, Štefan; Caron, N.; Spronck, L.; Vallés, A.; De Haan, M.; Blits, B.; Ellederová, Zdeňka; van Deventer, S.; Petry, H.; Southwell, A.; Déglon, N.; Motlík, Jan; Konstantinová, P.; Evers, M.
Ústav živočišné fyziologie a genetiky, 2017

Oxidative stress in primary porcine fibroblasts expressing mutated huntingtin
Šmatlíková, Petra; Askeland, G.; Vaškovičová, Michaela; Klíma, Jiří; Motlík, Jan; Eide, L.; Ellederová, Zdeňka
2017 - English
Molecular events, such as protein aggregation, mitochondrial dysfunction, and transcriptional dysregulation have been linked to Huntington’s disease (HD) pathogenesis. Oxidative stress has been considered as one of the key players in disease progression. Though, it is still not clear whether oxidative stress causes HD, or if it is a consequence of other primary events. Keywords: huntingtin Available at various institutes of the ASCR
Oxidative stress in primary porcine fibroblasts expressing mutated huntingtin

Molecular events, such as protein aggregation, mitochondrial dysfunction, and transcriptional dysregulation have been linked to Huntington’s disease (HD) pathogenesis. Oxidative stress has been ...

Šmatlíková, Petra; Askeland, G.; Vaškovičová, Michaela; Klíma, Jiří; Motlík, Jan; Eide, L.; Ellederová, Zdeňka
Ústav živočišné fyziologie a genetiky, 2017

Results of the realisation of the experiments using large experimental animals – application of cells/ stem cells using different vectors, diagnostics
Juhás, Štefan; Juhásová, Jana
2017 - English
A design of methods to test application of cells/ stem cells using different vectors, solution of the application including diagnostics and connected agenda. Keywords: large animal models; stem cell therapy; spinal stem cells Available at various institutes of the ASCR
Results of the realisation of the experiments using large experimental animals – application of cells/ stem cells using different vectors, diagnostics

A design of methods to test application of cells/ stem cells using different vectors, solution of the application including diagnostics and connected agenda.

Juhás, Štefan; Juhásová, Jana
Ústav živočišné fyziologie a genetiky, 2017

Mitochondrial phenotype in minipig model transgenic for N-terminal part of human mutated huntingtin
Hansíková, H.; Rodinová, M.; Křížová, J.; Dosoudilová, Z.; Štufková, H.; Bohuslavová, Božena; Klíma, Jiří; Juhás, Štefan; Ellederová, Zdeňka; Motlík, Jan; Zeman, J.
2017 - English
Huntington’s disease (HD) is neurodegenerative disorder caused by an abnormal expansion of CAG repeat encoding a polyglutamine tract of huntingtin (htt). It has been postulated that mitochondria dysfunction may play significant role in the pathophysiology of the HD. But it is still not known yet in detail how mitochondria are able to cover energy needs of the cells during the progression of the HD. Keywords: minipig model; Huntington´s disease Available at various institutes of the ASCR
Mitochondrial phenotype in minipig model transgenic for N-terminal part of human mutated huntingtin

Huntington’s disease (HD) is neurodegenerative disorder caused by an abnormal expansion of CAG repeat encoding a polyglutamine tract of huntingtin (htt). It has been postulated that mitochondria ...

Hansíková, H.; Rodinová, M.; Křížová, J.; Dosoudilová, Z.; Štufková, H.; Bohuslavová, Božena; Klíma, Jiří; Juhás, Štefan; Ellederová, Zdeňka; Motlík, Jan; Zeman, J.
Ústav živočišné fyziologie a genetiky, 2017

Double strand DNA breaks response in Huntington´s disease
Šolc, Petr
2017 - English
There are strong evidences that DNA damage response (DDR) signalling significantly underline the molecular pathology of polyglutamine (polyQ) diseases, including Huntington´s disease (HD) [1-4]. Double strand DNA breaks (DSBs) are the most deleterious DNA lesions.\nIn this talk I will review how DDR on DSBs is affected in HD.\n Keywords: DNA damage response Available at various institutes of the ASCR
Double strand DNA breaks response in Huntington´s disease

There are strong evidences that DNA damage response (DDR) signalling significantly underline the molecular pathology of polyglutamine (polyQ) diseases, including Huntington´s disease (HD) [1-4]. ...

Šolc, Petr
Ústav živočišné fyziologie a genetiky, 2017

Double strand DNA breaks response in Huntington´s disease transgenic minipigs
Vaškovičová, Michaela; Šmatlíková, Petra; Herbert, A.; Motlík, Jan; Šolc, Petr
2017 - English
Huntington’s disease (HD) is progressive neurodegenerative disorder caused by presence of CAG expansion in the huntingtin gene, which gives rise to mutated form of huntingtin protein (mHtt). There is a strong evidence that DNA damage response is compromised by presence of mHtt in cells and increase of double strand DNA breaks (DSBs) is an early event in HD pathology. It was shown, that level of γH2AX is significantly higher in R6/2 mice compared to wild-type animals. Moreover, level of γH2AX is higher also in striatal neurons and fibroblasts of human HD patients. Furthermore, protein p53, key player in DNA damage response, is hyperactivated in cells expressing mHtt and inhibition of p53 or ATM ameliorates phenotypes of HD animal models. However, exact mechanism of mHtt action is not clear and therefore further investigation of mHtt effects on DSBs response is very important for the understanding of HD pathology. Keywords: transgenic minipigs Available at various institutes of the ASCR
Double strand DNA breaks response in Huntington´s disease transgenic minipigs

Huntington’s disease (HD) is progressive neurodegenerative disorder caused by presence of CAG expansion in the huntingtin gene, which gives rise to mutated form of huntingtin protein (mHtt). There is ...

Vaškovičová, Michaela; Šmatlíková, Petra; Herbert, A.; Motlík, Jan; Šolc, Petr
Ústav živočišné fyziologie a genetiky, 2017

Role of FGF signaling in zeugopod development
Buchtová, Marcela; Celá, Petra; Horáková, D.; Krejčí, P.
2017 - English
The fibroblast growth factor (FGF) signalling systém regulates many developmental processes including skeletogenesis, brain patterning, branching morphogenesis or limb development. as FGFs are key players in the variable processes of chondrogenesis, we experimentally manipulated this pathway to test its effect on zeugopode modeling. Keywords: FGF Available at various institutes of the ASCR
Role of FGF signaling in zeugopod development

The fibroblast growth factor (FGF) signalling systém regulates many developmental processes including skeletogenesis, brain patterning, branching morphogenesis or limb development. as FGFs are key ...

Buchtová, Marcela; Celá, Petra; Horáková, D.; Krejčí, P.
Ústav živočišné fyziologie a genetiky, 2017

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