Number of found documents: 3
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Methodology for co-cryostorage of virus isolates in their host plants and relevant data
Bilavčík, A.; Bjerkestrand, R.; Bjelland, B.; Blystad, D.-R.; Bobrova, O.; Fránová, Jana; Faltus, M.; Hamborg, Z.; Hammond Hammond, S. D.; Koloniuk, Igor; Zámečník, J.
2023 - English
Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored. Keywords: genetic resources; virus; Rubus idaeus L. Fulltext is available at external website.
Methodology for co-cryostorage of virus isolates in their host plants and relevant data

Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is ...

Bilavčík, A.; Bjerkestrand, R.; Bjelland, B.; Blystad, D.-R.; Bobrova, O.; Fránová, Jana; Faltus, M.; Hamborg, Z.; Hammond Hammond, S. D.; Koloniuk, Igor; Zámečník, J.
Biologické centrum, 2023

Separace VRA/RA ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF) pro potřeby kontroly zákazu biologických zbraní
Kubíček, O.; Duša, Filip; Šalplachta, Jiří; Lunerová, K.; Rosenbergová, K.
2021 - English
Metodika popisuje způsob separace bakteriálních vysoce rizikových a rizikových agens (VRA/RA) ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF). Metoda chip-IEF využívá nově vyvinutý unikátní prototyp zařízení pro vertikální isoelektrickou fokusaci v rozbíhavém toku v čipovém provedení, která na rozdíl od dosavadních postupů umožňuje i separaci bakterií. Separace pomocí chip-IEF funguje i pro šetrně inaktivované bakterie, což významně snižuje bezpečnostní rizika při manipulaci se vzorkem potenciálně obsahujícím VRA/RA. Keywords: isoelectric focusing; B-agens; chip Fulltext is available at external website.
Separace VRA/RA ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF) pro potřeby kontroly zákazu biologických zbraní

Metodika popisuje způsob separace bakteriálních vysoce rizikových a rizikových agens (VRA/RA) ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF). Metoda chip-IEF využívá nově ...

Kubíček, O.; Duša, Filip; Šalplachta, Jiří; Lunerová, K.; Rosenbergová, K.
Ústav analytické chemie, 2021

Lapped scarf joints for reconstruction of historical structures
Kunecký, Jiří; Fajman, P.; Hasníková, Hana; Kuklík, P.; Kloiber, Michal; Sebera, V.; Tippner, J.
2016 - English
This paper presents a description of the repair of timber structures using a prosthesis scarf\njoint designed for the replacement of damaged parts of beams. This new scarf joint makes use\nof the strutting effect of inclined contact faces where the forces are transmitted through\nwooden coupling elements - wooden dowels or keys. The scarf can be modified in four\nvariants according to the relevant stress and is suitable for historically valuable timber\nstructures. It meets both functional and aesthetic requirements. The designer - structural\nengineer will learn in the methods the load bearing capacity or stiffness of the beam with the\ndesigned joint, its recommended dimensions and detailed geometry. The contractor will\nappreciate the description of the execution and maintenance of the joint. Keywords: carpentry joints; scarf joint; replacement; load bearing capacity; stiffness; joint execution Fulltext is available at external website.
Lapped scarf joints for reconstruction of historical structures

This paper presents a description of the repair of timber structures using a prosthesis scarf\njoint designed for the replacement of damaged parts of beams. This new scarf joint makes use\nof the ...

Kunecký, Jiří; Fajman, P.; Hasníková, Hana; Kuklík, P.; Kloiber, Michal; Sebera, V.; Tippner, J.
Ústav teoretické a aplikované mechaniky, 2016

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