Number of found documents: 80
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Different expression of house-keeping genes by quantitative real time RT-PCR
Ullmannová, V.; Truksa, Jaroslav; Haškovec, C.; Kovář, Jan
2003 - Czech
Keywords: quantitative real time RT-PCR; house-keeping genes expression Available at various institutes of the ASCR
Different expression of house-keeping genes by quantitative real time RT-PCR

Ullmannová, V.; Truksa, Jaroslav; Haškovec, C.; Kovář, Jan
Ústav molekulární genetiky, 2003

Estimation of the post-meiotic state of spermatogenesis in man by immunofluorescence with monoclonal antibodies against intra-acrosomal sperm proteins
Chládek, David; Pěknicová, Jana
2003 - English
Male infertility due to severe azoospermia has been associated with the absence of spermatozoa in ejaculated. We tested the ejaculates (IVF latoratory Pranatal Ltd) from 30 patients with azoospermia for the presence of cells from defferent stages of spermiogenesis. Monoclonal sntibodews against intraacrosomal human sperm proteins were used in the immunofluorescence test for detection of acrosomal proteins in the acrosomal precursors and acrosomes. Keywords: monoclonal antibody; acrosome Available at various institutes of the ASCR
Estimation of the post-meiotic state of spermatogenesis in man by immunofluorescence with monoclonal antibodies against intra-acrosomal sperm proteins

Male infertility due to severe azoospermia has been associated with the absence of spermatozoa in ejaculated. We tested the ejaculates (IVF latoratory Pranatal Ltd) from 30 patients with azoospermia ...

Chládek, David; Pěknicová, Jana
Ústav molekulární genetiky, 2003

Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J.; Dvořáková, K.; Pěknicová, Jana; Kubátová, Alena; Dráber, Pavel
2003 - English
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes. Keywords: cyprinus; carpio; acipenser Available at various institutes of the ASCR
Dynamic and role of cytosekeletal proteins in mammalian sperm cells

The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor ...

Paleček, J.; Dvořáková, K.; Pěknicová, Jana; Kubátová, Alena; Dráber, Pavel
Ústav molekulární genetiky, 2003

Regulace protizánětlivých imunitních reakcí v předním segmentu oka
Vítová, A.; Filipec, M.; Pindjáková, Jana; Krulová, Magdalena; Zajícová, Alena; Holáň, Vladimír
2003 - Czech
Rohovkové stroma bylo identifikováno jako zdroj nové molekuly zodpovědné za selektivní inhibici produkce protizánětlivých cytokinů. Corneal stroma was identified as a source of a novel molecule rosponsible for selective ingibitor of production of anti-inflammatory cytokines. Keywords: Rohovkové stroma; selektivní inhibice Available at various institutes of the ASCR
Regulace protizánětlivých imunitních reakcí v předním segmentu oka

Rohovkové stroma bylo identifikováno jako zdroj nové molekuly zodpovědné za selektivní inhibici produkce protizánětlivých cytokinů....

Vítová, A.; Filipec, M.; Pindjáková, Jana; Krulová, Magdalena; Zajícová, Alena; Holáň, Vladimír
Ústav molekulární genetiky, 2003

Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest
Král, Vlastimil; Fábry, Milan; Hořejší, Magdalena; Závada, Jan; Sedláček, Juraj
2003 - English
We describe molecular cloning, expression, purification and properties of two single chain antibody variable fragments (scFv) of potential diagnostic use, namely scFv M75 and scFv Tu-20. The former scFv is derived from a monoclonal antibody M75 specific for a cell surface protein MN/CA IX, strongly associated with many types of human carcinomas. The latter scFv is derived from a monoclonal antibody TU-20 specific for neuronal beta-III-tubulin. Keywords: cloning; purification; scFv; klonování; lékařské techniky Available at various institutes of the ASCR
Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest

We describe molecular cloning, expression, purification and properties of two single chain antibody variable fragments (scFv) of potential diagnostic use, namely scFv M75 and scFv Tu-20. The former ...

Král, Vlastimil; Fábry, Milan; Hořejší, Magdalena; Závada, Jan; Sedláček, Juraj
Ústav molekulární genetiky, 2003

Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Jelínková, Petra; Tichá, M.; Jonáková, Věra
2003 - English
Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not only spermadhesins, but also proteinase inhibitors participate in the formation of associated complexes. Proteinase inhibitors were found after gel chromatographic separation mainly in the fraction with the lowest relative molecular mass. Two different types of serine proteinase inhibitors were isolated from boar seminal plasma ů (i) glycosylated inhibitor with Mr 12 kDa, (ii) non-glycosylated sperm-associated acrosin inhibitor with Mr 8 kDa, and a new type of low molecular weight protein that is probably composed of two subunits coupled by the -S-S- bridge. The proteinase inhibitor with Mr 8 kDa was proved to form a heterodimer with AQN 1 spermadhesin under physiological conditions, whereas inhibitor with Mr 12 kDa forms homodimers. Keywords: proteinase inhibitors, aggregated forms, boar seminal plasma Available at various institutes of the ASCR
Characterization of peptide proteinase inhibitors isolated from boar seminal plasma

Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not ...

Jelínková, Petra; Tichá, M.; Jonáková, Věra
Ústav molekulární genetiky, 2003

Antitrophoblast cell-mediated immunity /AT-CMI/ in recurrent miscarriage and ectopic pregnacy
Madar, J.; Kučera, E.; Šůla, K.; Chaloupková, A.; Pěknicová, Jana; Tolarová, M.; Nouza, K.; Kinský, R.
2003 - English
Specific mechanisms operate in decidua nad trophoblast to establish and maintain immunological tolerance of the embryo in mother's uterus. due to the lack of this tolerance, disturbances of pregnancy may result. In this presentation, we have tested the hypothesis that anti-trohhoblast cell-mediated immunity (AT-CMI) is elevated in habitually aborting women and that extra-uterine localisation of the embryo leads to subsequent elevation of AT-CMI. Keywords: trophoblast; cardiolipin Available at various institutes of the ASCR
Antitrophoblast cell-mediated immunity /AT-CMI/ in recurrent miscarriage and ectopic pregnacy

Specific mechanisms operate in decidua nad trophoblast to establish and maintain immunological tolerance of the embryo in mother's uterus. due to the lack of this tolerance, disturbances of pregnancy ...

Madar, J.; Kučera, E.; Šůla, K.; Chaloupková, A.; Pěknicová, Jana; Tolarová, M.; Nouza, K.; Kinský, R.
Ústav molekulární genetiky, 2003

Multigenerational study - effect of the selected compounds on the mouse fertility
Boubelík, Michael; Pěknicová, Jana; Kyselová, Vendula; Buckiová, D.
2003 - English
Several compounds known to be effective for their influence onto the environment were tested. Compounds tested in this study were: benzo/a/ pyrene, lindane (hexachlorocyclohexane isomer), 4-n-nonylphenol, bisphenol A and octachlorostyrene. The question was if these compounds might also function as endocrine disruptors. Keywords: endocrine disruptors; sperm motility Available at various institutes of the ASCR
Multigenerational study - effect of the selected compounds on the mouse fertility

Several compounds known to be effective for their influence onto the environment were tested. Compounds tested in this study were: benzo/a/ pyrene, lindane (hexachlorocyclohexane isomer), ...

Boubelík, Michael; Pěknicová, Jana; Kyselová, Vendula; Buckiová, D.
Ústav molekulární genetiky, 2003

Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
Fučík, Vladimír; Šindelka, Radek; Beran, Jaroslav; Ferjentsik, Zoltán; Jonák, Jiří
2003 - English
In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in specific intervals. As the 31 bp interlink can assume a hairpin structure, the whole region can be viewed as an imperfect inverted repeat. The microsatellite was sequenced in imbred lines of M. m. musculus and M. m. domesticus, as well as in mice of both subspecies trapped near the hybrid zone. Differences in the length of repeat regions, insertion/deletions and simple base changes characterize the strains. Hybrids between the two species could be identified by sequencing of cloned PCR products. The structure of the microsatellite in more distant species was found to be conspicuously different. Instead of frequent (CA)18-25, we can see only (CA)8 in M. spretus, CATGCGCCCCCCA in Mus pahari and CACCCCCC in Mus caroli. No similar structure is present in the corresponding human src intron. Keywords: Mus musculus, c-src introns, microsatellite Available at various institutes of the ASCR
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution

In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in ...

Fučík, Vladimír; Šindelka, Radek; Beran, Jaroslav; Ferjentsik, Zoltán; Jonák, Jiří
Ústav molekulární genetiky, 2003

Imunita v oku jako modelu imunologicky privilegovaného místa
Zajícová, Alena; Pindjáková, Jana; Plšková, J.; Krulová, Magdalena; Filipec, M.; Holáň, Vladimír
2003 - Czech
Výsledky ukazují na významnou roli CD4+ T buněk, makrofágů a makrofágy produkovaného NO v procesu rejekce alo- i xenotransplantátů rohovek. The results show the significant role of CD4+ T cells, macrophages and NO produced by macophages during the rejection of the corneal allo- and xenografts. Keywords: cornea, rejection, cytokines; oko; cytokiny Available at various institutes of the ASCR
Imunita v oku jako modelu imunologicky privilegovaného místa

Výsledky ukazují na významnou roli CD4+ T buněk, makrofágů a makrofágy produkovaného NO v procesu rejekce alo- i xenotransplantátů rohovek....

Zajícová, Alena; Pindjáková, Jana; Plšková, J.; Krulová, Magdalena; Filipec, M.; Holáň, Vladimír
Ústav molekulární genetiky, 2003

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