Yes (1433)
No (5)
Number of found documents:
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Structural and functional characterization of the E3 ligase BIRC6
GRATZL, Sascha
2023 - English
The aim of the thesis was to characterize the ubiquitin ligase BIRC6 in several contexts. The goal was to expand the knowledge on the selectivity of E1-E2 recognition, to increase the known substrate range of BIRC6, and to elucidate the molecular interactions fulfilled by BIRC6 during autophagy via interaction with GABARAP. In addition, it was aimed to gain a better understanding on the general ubiquitination mechanism in E2/E3 hybrid enzymes, to identify the ubiquitination sites of known apoptotic and autophagic substrates and to enlighten substrate recognition by BIRC6. The aim of the thesis was to characterize the ubiquitin ligase BIRC6 in several contexts. The goal was to expand the knowledge on the selectivity of E1-E2 recognition, to increase the known substrate range of BIRC6, and to elucidate the molecular interactions fulfilled by BIRC6 during autophagy via interaction with GABARAP. In addition, it was aimed to gain a better understanding on the general ubiquitination mechanism in E2/E3 hybrid enzymes, to identify the ubiquitination sites of known apoptotic and autophagic substrates and to enlighten substrate recognition by BIRC6.
Keywords:
structural biology; cryo-EM; x-ray crystallography; autophagy; apoptosis; inhibitor of apoptosis proteins; AlphaFold2; ATG8; ubiquitin; E3 ligase
Available in the Digital Repository of University of South Bohemia.
Structural and functional characterization of the E3 ligase BIRC6
The aim of the thesis was to characterize the ubiquitin ligase BIRC6 in several contexts. The goal was to expand the knowledge on the selectivity of E1-E2 recognition, to increase the known substrate ...
Functional analysis of cryoprotectant system in Chymomyza costata
GRGAC, Robert
2023 - English
In this study, employing an integrated approach that involves transcriptomics, metabolomics, and other techniques, we aimed to understand the mechanistic roles of the cryoprotective system underlying extreme freeze tolerance in the larvae of the drosophilid fly, Chymomyza costata. Metabolomics unveiled the complex composition of the seasonally accumulated larval innate mixture of putative cryoprotectants. This mixture was predominantly composed of proline and trehalose, supplemented by minor components (glutamine, asparagine, betaine, sarcosine, glycerophospho-choline, and ethanolamine). We identified food ingestion as a significant source for the direct assimilation of amino compounds, while glycogen and phospholipids served as the primary internal sources for the biosynthesis of other cryoprotectants. The utilization of MALDI-MSI to observe cryoprotectants revealed distinct behaviors of proline and trehalose during ecologically relevant, gradual inoculative extracellular freezing of larvae. Trehalose exhibited accumulation in partially dehydrated hemolymph, inducing a transition to the amorphous glass phase. On the other hand, proline migrated to the boundary between extracellular ice and dehydrated hemolymph and tissues, forming a layer of dense viscoelastic liquid. Finally, through a combination of in vivo and in vitro assays, we found that cell membranes are likely targets of freezing injury, while their integrity is sustained by accumulated small cryoprotective molecules and proteins in cold-acclimated C. costata larvae. Contrary to our expectations, our assays did not provide support for the hypothesis that proteins (soluble enzymes) require in vivo stabilization through the accumulation of cryoprotectants. In this study, employing an integrated approach that involves transcriptomics, metabolomics, and other techniques, we aimed to understand the mechanistic roles of the cryoprotective system underlying extreme freeze tolerance in the larvae of the drosophilid fly, Chymomyza costata. Metabolomics unveiled the complex composition of the seasonally accumulated larval innate mixture of putative cryoprotectants. This mixture was predominantly composed of proline and trehalose, supplemented by minor components (glutamine, asparagine, betaine, sarcosine, glycerophospho-choline, and ethanolamine). We identified food ingestion as a significant source for the direct assimilation of amino compounds, while glycogen and phospholipids served as the primary internal sources for the biosynthesis of other cryoprotectants. The utilization of MALDI-MSI to observe cryoprotectants revealed distinct behaviors of proline and trehalose during ecologically relevant, gradual inoculative extracellular freezing of larvae. Trehalose exhibited accumulation in partially dehydrated hemolymph, inducing a transition to the amorphous glass phase. On the other hand, proline migrated to the boundary between extracellular ice and dehydrated hemolymph and tissues, forming a layer of dense viscoelastic liquid. Finally, through a combination of in vivo and in vitro assays, we found that cell membranes are likely targets of freezing injury, while their integrity is sustained by accumulated small cryoprotective molecules and proteins in cold-acclimated C. costata larvae. Contrary to our expectations, our assays did not provide support for the hypothesis that proteins (soluble enzymes) require in vivo stabilization through the accumulation of cryoprotectants.
Available in the Digital Repository of University of South Bohemia.
Functional analysis of cryoprotectant system in Chymomyza costata
In this study, employing an integrated approach that involves transcriptomics, metabolomics, and other techniques, we aimed to understand the mechanistic roles of the cryoprotective system underlying ...
Metabolic setup of Drosophila macrophages during the immune response
KREJČOVÁ, Gabriela
2023 - English
Adjustment of cellular metabolism is a key function that allows macrophages to fulfill their roles in the body. While the pro-inflammatory polarization of macrophages has been extensively studied in mammalian models, it has not yet been satisfactorily investigated in insects. The study presented in this thesis therefore attempts to elucidate the metabolic setup of macrophages during the immune response in Drosophila melanogaster. Adjustment of cellular metabolism is a key function that allows macrophages to fulfill their roles in the body. While the pro-inflammatory polarization of macrophages has been extensively studied in mammalian models, it has not yet been satisfactorily investigated in insects. The study presented in this thesis therefore attempts to elucidate the metabolic setup of macrophages during the immune response in Drosophila melanogaster.
Keywords:
makrofág; polarizace; metabolismus; infekce; octomilka; plazmatocyt; imunitní odpověď
Available in the Digital Repository of University of South Bohemia.
Metabolic setup of Drosophila macrophages during the immune response
Adjustment of cellular metabolism is a key function that allows macrophages to fulfill their roles in the body. While the pro-inflammatory polarization of macrophages has been extensively studied in ...
Red-backed shrike (Lanius collurio) versus common cuckoo (Cuculus canorus): An example of ineffective cuckoo-hawk mimicry
KRAUSOVÁ, Ladislava
2023 - English
V této práci jsem testovala reakci ťuhýka obecného (Lanius collurio) na hnízdního parazita, kukačku obecnou (Cuculus canorus) (šedá forma), predátora, krahujce obecného (Accipiter nisus) a neškodnou kontrolu, hrdličku divokou (Streptoptelia turtur). Prokázala jsem, že ťuhýci od sebe dokážou rozlišit jednotlivé atrapy a adekvátně na ně reagovat. Navíc jsem prokázala, že imitace krahujce kukačkou je u ťuhýků neúspěšná. In this work, I tested the reaction of the red-backed shrike (Lanius collurio) against a nest parasite, the common cuckoo (Common cuckoo) (grey form), predator, Eurasian sparrowhawk (Accipiter nisus) and harmless intruder, turtle dove (Streptopelia turtur). I proved that shrikes can differ between presented stimuli and adequately respond to them. Moreover, I proved that the cuckoo-hawk mimicry is not successful against shrikes.
Keywords:
ťuhýk obecný; cuckoo-hawk mimicry; hnízdní parazitismus; obrana hnízda
Available in the Digital Repository of University of South Bohemia.
Red-backed shrike (Lanius collurio) versus common cuckoo (Cuculus canorus): An example of ineffective cuckoo-hawk mimicry
V této práci jsem testovala reakci ťuhýka obecného (Lanius collurio) na hnízdního parazita, kukačku obecnou (Cuculus canorus) (šedá forma), predátora, krahujce obecného (Accipiter nisus) a neškodnou ...
The role of macrophages in the regulation of systemic metabolism in Drosophila
KREJČOVÁ, Gabriela
2023 - English
Macrophages are immensely versatile cells in the mammalian body, fulfilling roles ranging from protection against pathogenic intruders and engulfing apoptotic cells to morphogenesis and maintenance of tissue homeostasis. This impressive functional versatility may be achieved due to plasticity of macrophage cellular metabolism called metabolic polarization. The adoption of different polarization phenotypes by macrophages determines their function and is essential for the health of the organism. Nonetheless, if the cells lose their metabolic plasticity or polarize inadequately to a particular situation, it can lead to the development of chronic pathological states such as metabolic syndrome. Metabolic polarization of immune cells is thus a key factor in determining whether macrophage function within the organism will be adaptive or pathological. Despite Drosophila melanogaster represents a major model organism for immunological studies, the metabolic setup of activated immune cells has not been addressed up to now. The results of this thesis document that Drosophila immune cells undergo metabolic polarization toward aerobic glycolysis when challenged by extracellular bacteria. Mammals alike, this cellular metabolic switch is regulated by the transcription factor HIF1, thus documenting the conservation of this process between insects and vertebrates. Furthermore, we show that the adoption of aerobic glycolysis is directly linked to the production of the signaling factor IMPL2, which induces the mobilization of lipid stores from the fat body via the silencing of insulin signaling. By this mechanism, immune cells secure sufficient nutrients for successful elimination of the pathogen. Moreover, the mammalian ImpL2 homolog IGFBP7 appears to act analogously in the mammalian liver not only during severe infectious states but also in the liver of obese individuals. While such macrophage activity in regulating systemic metabolism is beneficial to the host during bacterial infection, it becomes maladaptive when chronically activated. Further evidence for a metabolism-regulatory role of immune cells has been found during insect metamorphosis and early post-metamorphic development. This thesis documents that during this period, macrophages infiltrate and engulf the histolyzing larval fat body and convert nutrients into storage peptides and lipoproteins. Subsequently, these nutrients are exploited by the maturing adult structures. Macrophages are immensely versatile cells in the mammalian body, fulfilling roles ranging from protection against pathogenic intruders and engulfing apoptotic cells to morphogenesis and maintenance of tissue homeostasis. This impressive functional versatility may be achieved due to plasticity of macrophage cellular metabolism called metabolic polarization. The adoption of different polarization phenotypes by macrophages determines their function and is essential for the health of the organism. Nonetheless, if the cells lose their metabolic plasticity or polarize inadequately to a particular situation, it can lead to the development of chronic pathological states such as metabolic syndrome. Metabolic polarization of immune cells is thus a key factor in determining whether macrophage function within the organism will be adaptive or pathological. Despite Drosophila melanogaster represents a major model organism for immunological studies, the metabolic setup of activated immune cells has not been addressed up to now. The results of this thesis document that Drosophila immune cells undergo metabolic polarization toward aerobic glycolysis when challenged by extracellular bacteria. Mammals alike, this cellular metabolic switch is regulated by the transcription factor HIF1, thus documenting the conservation of this process between insects and vertebrates. Furthermore, we show that the adoption of aerobic glycolysis is directly linked to the production of the signaling factor IMPL2, which induces the mobilization of lipid stores from the fat body via the silencing of insulin signaling. By this mechanism, immune cells secure sufficient nutrients for successful elimination of the pathogen. Moreover, the mammalian ImpL2 homolog IGFBP7 appears to act analogously in the mammalian liver not only during severe infectious states but also in the liver of obese individuals. While such macrophage activity in regulating systemic metabolism is beneficial to the host during bacterial infection, it becomes maladaptive when chronically activated. Further evidence for a metabolism-regulatory role of immune cells has been found during insect metamorphosis and early post-metamorphic development. This thesis documents that during this period, macrophages infiltrate and engulf the histolyzing larval fat body and convert nutrients into storage peptides and lipoproteins. Subsequently, these nutrients are exploited by the maturing adult structures.
Keywords:
makrofág; metabolismus; imunitní odpověď; polarizace; ImpL2; octomilka; tukové těleso; infiltrace; insulinová rezistence; glukanové částice
Available in the Digital Repository of University of South Bohemia.
The role of macrophages in the regulation of systemic metabolism in Drosophila
Macrophages are immensely versatile cells in the mammalian body, fulfilling roles ranging from protection against pathogenic intruders and engulfing apoptotic cells to morphogenesis and maintenance of ...
Carbohydrates drive growth and longevity tradeoff strategies of plants in the Western United States
SAMRAOUI, Kenz Raouf
2023 - English
This study explores the relationship between non-structural carbohydrates (NSCs), growth, and longevity in over 200 vascular plant species across diverse Western U.S. habitats. The research identifies specific roles for NSC compoundsfructans enhance longevity, while simple sugars support rapid growth at the expense of longevity. By elucidating the roles of individual NSC compounds and their relationship with growth and longevity, we are contributing to a deeper understanding of plant adaptation and survival strategies. This study explores the relationship between non-structural carbohydrates (NSCs), growth, and longevity in over 200 vascular plant species across diverse Western U.S. habitats. The research identifies specific roles for NSC compoundsfructans enhance longevity, while simple sugars support rapid growth at the expense of longevity. By elucidating the roles of individual NSC compounds and their relationship with growth and longevity, we are contributing to a deeper understanding of plant adaptation and survival strategies.
Keywords:
Carbon allocation strategies; Environmental adaptation; Non-structural carbohydrates (NSCs); Plant growth dynamics; United States of America.
Available in the Digital Repository of University of South Bohemia.
Carbohydrates drive growth and longevity tradeoff strategies of plants in the Western United States
This study explores the relationship between non-structural carbohydrates (NSCs), growth, and longevity in over 200 vascular plant species across diverse Western U.S. habitats. The research identifies ...
Association between personality traits and cognitive abilities in jumping spiders
PROSKURIAKOVA, Kseniia
2023 - English
I measured two personality traits in jumping spiders Phidippus regius, boldness and explorativeness, to identify the presence of these traits and their repeatability. Also I analysed the correlation between explorativeness and boldness, and the correlation between these traits and learning abilities of Salticidae spiders. I measured two personality traits in jumping spiders Phidippus regius, boldness and explorativeness, to identify the presence of these traits and their repeatability. Also I analysed the correlation between explorativeness and boldness, and the correlation between these traits and learning abilities of Salticidae spiders.
Keywords:
Jumping spiders; Phidippus regius; Salticidae Personality traits; Boldness; Exploration latency; Repeatability; Learning abilities
Available in the Digital Repository of University of South Bohemia.
Association between personality traits and cognitive abilities in jumping spiders
I measured two personality traits in jumping spiders Phidippus regius, boldness and explorativeness, to identify the presence of these traits and their repeatability. Also I analysed the correlation ...
Red-backed shrike (\kur{Lanius collurio}) versus Common cuckoo (\kur{Cuculus canorus}): Clutch variability and egg recognition
KRAUSOVÁ, Ladislava
2023 - English
V této práci jsem pomocí fotografie testoval variabilitu vajec ťuhýka obecného (Lanius collurio) uvnitř snůšky a porovnával ji s variabilitou vajec mezi snůškami. Byl hodnocen vliv barvy, velikosti, objemu a makulace a chromatických a achromatických kontrastů. Dále jsem testovala schopnost ťuhýka obecného rozpoznávat umělá kukaččí vejce a umělá vejce ťuhýka obecného, vyhodnotila jsem míru odmítání těchto vajec a porovnala ji s mírou odmítání konspecifických vajec. Ukázala jsem, že u všech měřených parametrů mají ťuhýci nízkou vnitrosnůškovou variabilitu a vysokou mezisnůškovou variabilitu. Tato variabilita může být adaptací proti hnízdnímu parazitismu. Kromě toho jsem zjistila, že schopnost ťuhýků rozpoznat a odmítnout parazitické vejce je výrazně vyšší, než se očekávalo. In this work, using photography, I tested the red-backed shrike (Lanius collurio) intraclutch egg variability and compared it with the interclutch egg variability. The effect of colour, size, volume and maculation as well as chromatic and achromatic contrasts were evaluated. Moreover, I tested ability of the red-backed shrike to recognize artificial cuckoo eggs and artificial shrike eggs, evaluate the rejection rate of these eggs, and compare it with the rejection rate of conspecific eggs. I showed that shrikes have low intraclutch variability and high interclutch variability in all measured parameters. Such variability might be an adaptation against brood parasitism. In addition, I found out that the shrikes' ability to recognize and reject a parasitic egg is significantly higher than expected.
Keywords:
Red-backed shrike; egg variability; parasitism; egg rejection
Available in the Digital Repository of University of South Bohemia.
Red-backed shrike (\kur{Lanius collurio}) versus Common cuckoo (\kur{Cuculus canorus}): Clutch variability and egg recognition
V této práci jsem pomocí fotografie testoval variabilitu vajec ťuhýka obecného (Lanius collurio) uvnitř snůšky a porovnával ji s variabilitou vajec mezi snůškami. Byl hodnocen vliv barvy, velikosti, ...
Intraspecific trait variability of herbaceous plants in organic and conventional olive plantations in region at desertification risk
MIGLIORINO, Michele
2023 - English
This thesis is an ecological study in an agricultural context (agroecological research). It is carried out in olive plantations in south Italy, Apulia, a region at strong desertification risk. The effect of agricultural management (organic vs. conventional) is investigated on i) the herbaceous species composition ii) intraspecific trait variability of five selected herbaceous plants iii) soil conditions. Herbaceous plants are here considered as "indicators" of olive plantations' health. Studying if and how species traits and soil parameters vary with management, should reveal how olive understory grasslands are responding to increasing drought conditions and other desertification constraints. Management of grasslands and soils is critical to maintain high ecological functionality and services of olive agroecosystems. This thesis is an ecological study in an agricultural context (agroecological research). It is carried out in olive plantations in south Italy, Apulia, a region at strong desertification risk. The effect of agricultural management (organic vs. conventional) is investigated on i) the herbaceous species composition ii) intraspecific trait variability of five selected herbaceous plants iii) soil conditions. Herbaceous plants are here considered as "indicators" of olive plantations' health. Studying if and how species traits and soil parameters vary with management, should reveal how olive understory grasslands are responding to increasing drought conditions and other desertification constraints. Management of grasslands and soils is critical to maintain high ecological functionality and services of olive agroecosystems.
Keywords:
intraspecific trait variability; grasslands; organic agriculture; herbaceous plants; functional traits; olive plantations; agroecosystems; desertification; drought; aridity
Available in the Digital Repository of University of South Bohemia.
Intraspecific trait variability of herbaceous plants in organic and conventional olive plantations in region at desertification risk
This thesis is an ecological study in an agricultural context (agroecological research). It is carried out in olive plantations in south Italy, Apulia, a region at strong desertification risk. The ...
Dawn chorus succession in a lowland rainforest of Papua New Guinea
MEDELLÍN BECERRA, Sofía
2023 - English
This thesis examines the patterns and ecological significance of bird vocalizations during the dawn chorus in the rainforests of Papua New Guinea. Specifically, it aims to identify the species involved, analyze their songs, and understand the influence of environmental factors. To accomplish this, the study employs a combination of field recordings, phylogenetic analysis, and statistical methods to elucidate the complex interactions between avian species and their habitats. The findings of this study contribute to the broader understanding of biodiversity and ecosystem dynamics in tropical rainforests. This thesis examines the patterns and ecological significance of bird vocalizations during the dawn chorus in the rainforests of Papua New Guinea. Specifically, it aims to identify the species involved, analyze their songs, and understand the influence of environmental factors. To accomplish this, the study employs a combination of field recordings, phylogenetic analysis, and statistical methods to elucidate the complex interactions between avian species and their habitats. The findings of this study contribute to the broader understanding of biodiversity and ecosystem dynamics in tropical rainforests.
Keywords:
Bioacoustics; Papua New Guinea; Dawn Chorus; Birds; Ecology; Biodiversity
Available in the Digital Repository of University of South Bohemia.
Dawn chorus succession in a lowland rainforest of Papua New Guinea
This thesis examines the patterns and ecological significance of bird vocalizations during the dawn chorus in the rainforests of Papua New Guinea. Specifically, it aims to identify the species ...
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