Number of found documents: 47
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The ubiquitin–proteasome system is involved in the regulation of activity of spermadhesin aqn1 and acrosin inhibitor, the two sperm surface proteins, during porcine fertilization
Jonáková, Věra; Yi, Y.J.; Postlerová, Pavla; Pěknicová, Jana
2014 - English
The spermadhesin AQN1and acrosin inhibitor (AI/SPINK2) proteins bind to the sperm plasma membrane at ejaculation. The AQN1 has been implicated in sperm binding to zona pellucida (ZP) of the oocyte as well as in sperm interactions with the epithelium of the oviductal sperm reservoir. The SPINK2 protects spermatozoa from proteolytic degradation during their trip up the female genital tract toward the oocyte. This study examined the role of two components of the 19S proteasome regulatory complex, the ubiquitin C-terminal hydrolase UCHL3 and PSMD8 in the AQN1-mediated boar sperm binding to zona pellucida. Interaction of PSMD4 subunit with the acrosomal surface-associated acrosin inhibitor AI/SPINK2 provided another line of evidence for the presence of 26S proteasomes on the sperm surface. Detection of the ubiquitinated forms of SPINK2 supports the hypothesis that SPINK2 activity is controlled by ubiquitin-proteasome system (UPS). The activity of the porcine AQN1, and thus the efficiency of sperm-oocyte recognition/binding, may be controlled by elements of the sperm surface-bound UPS, in particular by UCHL3, and by proteasomal regulatory complex subunit PSMD8. Ubiquitinated isoforms of AQN1 were also detected in boar sperm extracts. The UCHL inhibitor ubiquitin aldehyde and the antibodies against UCHL3 or PSMD8 increased the rate of sperm-ZP penetration and polyspermy during porcine in vitro fertilization (IVF). In contrast, the addition of recombinant UCHL3 to fertilization medium significantly reduced polyspermy rates, while maintaining satisfactory rate of monospermic fertilization (~50%). These results are significant for production agriculture. The high level of polyspermy that hinders porcine IVF for commercial embryo transfer could be mitigated by the modulation of the UCHL3 and/or PSMD8 activity. Keywords: ubiquitin-proteasome system; acrosin inhibitor; spermadhesin AQN1 Available at various institutes of the ASCR
The ubiquitin–proteasome system is involved in the regulation of activity of spermadhesin aqn1 and acrosin inhibitor, the two sperm surface proteins, during porcine fertilization

The spermadhesin AQN1and acrosin inhibitor (AI/SPINK2) proteins bind to the sperm plasma membrane at ejaculation. The AQN1 has been implicated in sperm binding to zona pellucida (ZP) of the oocyte as ...

Jonáková, Věra; Yi, Y.J.; Postlerová, Pavla; Pěknicová, Jana
Biotechnologický ústav, 2014

Endocrine disruptors induce transgenerational alterations of the male reproductive parameters and mirna expression profiles in mouse primordial germ cells
Děd, Lukáš; Brieno-Enríquez, M.A.; García-López, J.; Cárdenas, D.B.; Guibert, S.; Hourcade, J.de D.; Pěknicová, Jana; Weber, M.; del Mazo, J.
2014 - English
Primordial germ cells (PGCs) are the embryonic precursors of the germ cell linage, which are restricted to form only sperm and oocytes following their specification from pluripotent cells. PGC precursors are specified in the epiblast around 6.25 days post coitum (dpc), and around 7.25 dpc become identifiable in a 40 cell-cluster. In the present study, we used a mouse model to evaluate the trans-generational (F1-F3) effects of vinclozolin (VCZ) administrated in two doses on male reproductive parameters. We observed decreased fertility rate, higher apoptotic rate and histopathologic alterations in adult testis, PGC number reduction, increments of PGCs apoptosis and changes in PGCs gene expression among all three generations. In the attempt to clarify the trans-generational transmition of the altered phenotypes, we performed the microRNA expression and DNA methylation analysis. We observed the significant alteration in the expression of multiple microRNA and microRNA-regulated genes which are important for PGCs specification, including LIN28, let-7 and BLIMP1. Trans-generational deregulation in the expression of factors involved in the Lin28-let-7-Blimp1 pathway can lead to specific VCZ-induced phenotype observed in our study. Keywords: endocrine disruptor; miRNA expression; primordial germ cells; vinclozolin; DNA methylation Available at various institutes of the ASCR
Endocrine disruptors induce transgenerational alterations of the male reproductive parameters and mirna expression profiles in mouse primordial germ cells

Primordial germ cells (PGCs) are the embryonic precursors of the germ cell linage, which are restricted to form only sperm and oocytes following their specification from pluripotent cells. PGC ...

Děd, Lukáš; Brieno-Enríquez, M.A.; García-López, J.; Cárdenas, D.B.; Guibert, S.; Hourcade, J.de D.; Pěknicová, Jana; Weber, M.; del Mazo, J.
Biotechnologický ústav, 2014

Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation
Pěknicová, Jana; Kubátová, Alena; Elzeinová, Fatima
2014 - English
Book of abstracts. The Symposium was focused on immunology of reproduction and specific problems in reproduction (mainly in human infertility). Keywords: reproduction; immunoglobulins; monoclonal antibody; endocrine disruptors; oocytes; infection Available at various institutes of the ASCR
Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation

Book of abstracts. The Symposium was focused on immunology of reproduction and specific problems in reproduction (mainly in human infertility).

Pěknicová, Jana; Kubátová, Alena; Elzeinová, Fatima
Biotechnologický ústav, 2014

Konferenční sborník XVIII. symposium českých reprodukčních imunologů s mezinárodní účastí
Pěknicová, Jana; Elzeinová, Fatima; Kubátová, Alena
2012 - English
Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation contains abstracts of lectures which focuse on immunology and physiology of reproduction. Keywords: immunology of reproduction; autoimmune diseases; antibodies; spermatozoa; oocytes; proteins; endocrine disruptors Available at various institutes of the ASCR
Konferenční sborník XVIII. symposium českých reprodukčních imunologů s mezinárodní účastí

Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation contains abstracts of lectures which focuse on immunology and physiology of reproduction.

Pěknicová, Jana; Elzeinová, Fatima; Kubátová, Alena
Biotechnologický ústav, 2012

METODICKÉ DOPORUČENÍ PRO VYKAZOVÁNÍ SKUTEČNÝCH ZPŮSOBILÝCH NEPŘÍMÝCH NÁKLADŮ NA PROJEKT PRO ÚSTAVY AV ČR
Škoda, Jan; Starosta, Karel
2012 - Czech
Metodické doporučení si klade za cíl formulovat společné možné zásady pro pracoviště AV ČR týkající se alokace nepřímých nákladů na projekty a to především s ohledem na pravidla poskytovatelů, kteří přiřazování nepřímých nákladů metodou full cost vyžadují. Metodika vychází z obvyklých hospodářských a účetních postupů ústavů AV ČR. Navrhované řešení je založeno na maximální jednoduchosti a efektivnosti s cílem minimálního navýšení nepřímých nákladů zapříčiněných užíváním této metodiky, umožňuje zakončení hospodářského roku v řádném termínu a vykázání potřebných dat poskytovatelům. The aim of the Methodical Recommendation is to create possible common principles for institutes of the Academy of Sciences of the Czech Republic (AS CR) concerning the allocation of indirect costs to projects, considering mainly the rules of fund providers requesting reporting of real costs via full cost methodology. Proposed methodology is based on usual management and accounting practices of institutes of AS CR and lie in principles of maximum simplicity and effectiveness. It should result in minimum increase of real indirect costs caused by application of such methodology, it enables closing of economic year on time and also reporting of necessary data to fund providers. Keywords: AS CR; overheads; indirect costs; full cost Available at various institutes of the ASCR
METODICKÉ DOPORUČENÍ PRO VYKAZOVÁNÍ SKUTEČNÝCH ZPŮSOBILÝCH NEPŘÍMÝCH NÁKLADŮ NA PROJEKT PRO ÚSTAVY AV ČR

Metodické doporučení si klade za cíl formulovat společné možné zásady pro pracoviště AV ČR týkající se alokace nepřímých nákladů na projekty a to především s ohledem na pravidla poskytovatelů, kteří ...

Škoda, Jan; Starosta, Karel
Biotechnologický ústav, 2012

Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation
Pěknicová, Jana; Elzeinová, Fatima; Cozlová, Nina
2011 - English
Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation contains abstracts of lectures which focuse on immunology and physiology of reproduction. Keywords: immunology of reproduction; autoimmune diseases; antibodies; spermatozoa; oocytes; proteins; endocrine disruptors Available at various institutes of the ASCR
Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation

Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation contains abstracts of lectures which focuse on immunology and physiology of reproduction.

Pěknicová, Jana; Elzeinová, Fatima; Cozlová, Nina
Biotechnologický ústav, 2011

Vliv endokrinních disruptorů na reprodukci savců
Pěknicová, Jana; Nováková, Vendula; Buckiová, Daniela; Boubelík, Michael; Elzeinová, Fatima; Kubátová, Alena
2008 - Czech
Endocrine system is one of the most important regulatory systems in the mammalian body. The endocrine disruptors (ED) can disrupt the function of the endocrine system but their activity is not a result of the physiological regulation. The effect of low dose of selected ED, bisphenol-A (BPA), p-nonylphenol (NP), diethylstilbestrol (DES), genistein (GEN), resveratrol (RES)), hormone (17α-ethynylestradiol (EE2)) and polybrominated compound (tetrabromobisphenol A (TBBPA)) within development of reproductive tract in outbred CD1 mice was tested. BPA and NP had a negative effect on reproductive organs morphology and mouse spermatozoa. The fertility of mice exposed to DES was disrupted. Contrary to that, treatment by GEN and RES had no effect on the fertility of mice but had some effect on their reproductive parameters except for sperm integrity. Synthetic hormone (EE2) and polybrominated compound (TBBPA) also affected on the organ weight, reproductive parameters and sperm quality. Keywords: endocrine disruptor; xenoestrogen; phytoestrogen; sperm integrity; endokrinní systém; plodnost Available at various institutes of the ASCR
Vliv endokrinních disruptorů na reprodukci savců

Endocrine system is one of the most important regulatory systems in the mammalian body. The endocrine disruptors (ED) can disrupt the function of the endocrine system but their activity is not a ...

Pěknicová, Jana; Nováková, Vendula; Buckiová, Daniela; Boubelík, Michael; Elzeinová, Fatima; Kubátová, Alena
Biotechnologický ústav, 2008

Monoklonální protilátky rozpoznávající proteiny lidské semenné plazmy
Čapková, Jana; Elzeinová, Fatima; Margaryan, Hasmik; Koubek, Pavel; Pěknicová, Jana
2008 - Czech
Ustavili jsme panel monoklonálních protilátek protil lidským spermiím (Hs- serie, Praha) a charakterizovali jsme je pomocí imunofluorescence, Western bloting techniky a MALDI analýzy. V našem panelu Hs- protilátek několik protilátek reagovalo s proteiny lidské semenné plasmy. Tyto protilátky mohou být užitečné pro studium kapacitace spermií a hodnocení kvality spermatu. We have generated a set of monoclonal antibodies to human spermatozoa (Hs- serie, Prague) and characterized them using immunofluorescence, Western bloting and MALDI analysis. In our panel of Hs- antibodies several antibodies specifically reacted with human seminal plasma proteins. These antibodies might be useful for study sperm capacitation and sperm quality assessment. Keywords: monoclonal antibody; seminal plasma; spermatozoa Available at various institutes of the ASCR
Monoklonální protilátky rozpoznávající proteiny lidské semenné plazmy

Ustavili jsme panel monoklonálních protilátek protil lidským spermiím (Hs- serie, Praha) a charakterizovali jsme je pomocí imunofluorescence, Western bloting techniky a MALDI analýzy. V našem panelu ...

Čapková, Jana; Elzeinová, Fatima; Margaryan, Hasmik; Koubek, Pavel; Pěknicová, Jana
Biotechnologický ústav, 2008

The effect of low dose of vinclozolin on reproductive tract development in CD1 outbred mice
Elzeinová, Fatima; Pěknicová, Jana; Nováková, Vendula; Buckiová, Daniela; Kubátová, Alena
2008 - English
The effect of a low dose of vinclozolin within the development of the reproductive tract during gestation (VIN-GD 15-22) and puberty (VIN-PND 23-44) in CD1 mice was tested. We found a decrease in the anogenital distance, prostate weight and pathology of testes in both experimental groups. Sperm counts decreased to 46% (VIN-GD) and to 81% (VIN-PND), and also the acrosomal state (evaluated by antiacrosomal antibody) decreased in both groups to 89% in comparison to the control group (100%). Sperm head abnormalities increased by approximately 18% and 13%, respectively. In this connection, the expression of some genes was changed (arosome-related gene (Acr), apoptosis related genes (p53, p21)). In conclusion, a low dose of vinclozolin affected the reproductive tract, sperm parameters and expression of selected genes in both experimental groups. Keywords: vinclozolin; CD1 mouse; spermatozoa Available at various institutes of the ASCR
The effect of low dose of vinclozolin on reproductive tract development in CD1 outbred mice

The effect of a low dose of vinclozolin within the development of the reproductive tract during gestation (VIN-GD 15-22) and puberty (VIN-PND 23-44) in CD1 mice was tested. We found a decrease in the ...

Elzeinová, Fatima; Pěknicová, Jana; Nováková, Vendula; Buckiová, Daniela; Kubátová, Alena
Biotechnologický ústav, 2008

Differences in the strategies of mammalian and fish fertilization and the optimization of the protocol for immunofluorescence on fish spermatozoa
Koubek, Pavel; Králová, Alena; Pšenička, M.; Pěknicová, Jana
2008 - English
This lecture describes a special approach to handling fish spermatozoa in comparison with the symplex originating from mammals. This approach primarily emphasizes the concrete parts of immunofluorescence protocol determining accurate results. Vzhledem ke struktuře rybích spermií, která je jednoduchá a snadno poškoditelná, bylo třeba vypracovat protokol pro imunofluorescenční značení, který by minimalizoval jejich poškození a vznik nespecifických artefaktů. Cílem práce tak bylo především zamezit mylné interpretaci výsledků imunofluorescenčních experimentů na rybích spermiích a zvýšit vzájemnou porovnatelnost těchto experimentů. Keywords: fish spermatozoa; mammalian spermatozoa; immunofluorescence Available at various institutes of the ASCR
Differences in the strategies of mammalian and fish fertilization and the optimization of the protocol for immunofluorescence on fish spermatozoa

This lecture describes a special approach to handling fish spermatozoa in comparison with the symplex originating from mammals. This approach primarily emphasizes the concrete parts of ...

Koubek, Pavel; Králová, Alena; Pšenička, M.; Pěknicová, Jana
Biotechnologický ústav, 2008

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