Characterization of pore opening relevant residues in TM3 and TM4 domains of Orai1
ANDOVA, Ana-Marija
2022 - English Calcium (Ca2+) ions play a crucial role in almost every aspect of cellular life. The most prominent calcium entry pathway into the cell is the calcium release-activated calcium (CRAC) channel, composed of the Orai1 protein, and the stromal interaction molecule STIM1. The channel is activated through conformational changes upon STIM1 coupling to the C-terminus of Orai1 protein following store depletion, which in turn allows Ca2+ influx into the cell. The abnormal function of the CRAC channel caused by mutations gives rise to distinct pathologies. Since it has not yet been elucidated how the signal propagation moves to the pore upon coupling, this thesis dives into its investigation by focusing on characterizing the TM3 and TM4 domains and their importance in leading to an open permissive conformation of the channel. The pivotal foundation for the creation of novel strategies in the modulation of the Orai1 function lies with the understanding of the dynamics of the Orai1 pore opening. Calcium (Ca2+) ions play a crucial role in almost every aspect of cellular life. The most prominent calcium entry pathway into the cell is the calcium release-activated calcium (CRAC) channel, composed of the Orai1 protein, and the stromal interaction molecule STIM1. The channel is activated through conformational changes upon STIM1 coupling to the C-terminus of Orai1 protein following store depletion, which in turn allows Ca2+ influx into the cell. The abnormal function of the CRAC channel caused by mutations gives rise to distinct pathologies. Since it has not yet been elucidated how the signal propagation moves to the pore upon coupling, this thesis dives into its investigation by focusing on characterizing the TM3 and TM4 domains and their importance in leading to an open permissive conformation of the channel. The pivotal foundation for the creation of novel strategies in the modulation of the Orai1 function lies with the understanding of the dynamics of the Orai1 pore opening. Keywords: ion channel; calcium signaling; CRAC channels; channel; CRAC; Orai1; Orai protein; STIM1; STIM protein; CRAC channel activation; molecular biology; electrophysiology; cell culture; mutation; TM domain; TM3; TM4; SOCE; pore opening; transmembrane domain Available in the Digital Repository of University of South Bohemia. Characterization of pore opening relevant residues in TM3 and TM4 domains of Orai1

Calcium (Ca2+) ions play a crucial role in almost every aspect of cellular life. The most prominent calcium entry pathway into the cell is the calcium release-activated calcium (CRAC) channel, ...

ANDOVA, Ana-Marija
Jihočeská univerzita v Českých Budějovicích, 2022

Phytolith analysis at the Neolithic tell Vrbjanska Čuka in Pelagonia (North Macedonia)
BUDILOVÁ, Kristýna
2022 - English The thesis deals with an analysis of phytoliths, microscopical residues of plants. The analysis was applied in order to gain a new archaeobotanical data on the Neolithic period at the archaeological site Vrbjanska Čuka in Pelagonia, North Macedonia. The results brought new insights into a plant management and related activities on the site, as well as contributed to an understanding of a past environment of the early agricultural society. The thesis deals with an analysis of phytoliths, microscopical residues of plants. The analysis was applied in order to gain a new archaeobotanical data on the Neolithic period at the archaeological site Vrbjanska Čuka in Pelagonia, North Macedonia. The results brought new insights into a plant management and related activities on the site, as well as contributed to an understanding of a past environment of the early agricultural society. Keywords: phytoliths; archaeobotany; Neolithic; Balkans Available in the Digital Repository of University of South Bohemia. Phytolith analysis at the Neolithic tell Vrbjanska Čuka in Pelagonia (North Macedonia)

The thesis deals with an analysis of phytoliths, microscopical residues of plants. The analysis was applied in order to gain a new archaeobotanical data on the Neolithic period at the archaeological ...

BUDILOVÁ, Kristýna
Jihočeská univerzita v Českých Budějovicích, 2022

Seizing the past in the Novels of Julian Barnes with Regard to the Author's Narrative Techniques
ČEŠKOVÁ, Karolína
2022 - English Diplomová práce zkoumá pojetí minulosti ve vybraných dílech Juliana Barnese, přičemž tento jev uvádí do souvislosti s narativními technikami, které autor zvolil. Práce se zaměřuje na ústřední tematické okruhy: beletrizování historie a obousměrnou povahu minulosti a současnosti. Vytyčené okruhy odrážejí autorův přístup k uchopení tématu minulosti v jeho románech. Cílem práce je nejen rozebrat téma hledání historické pravdy, které je společné Barnesovům dílům, ale také vyobrazení minulosti ve vztahu k tématům paměti, pravdy a vlivu těchto faktorů na formování identity jedince i kolektivu. Zohledněna je nejen narativní, ale i tematická inspirace, kterou Barnesově současnému psaní poskytly postmoderní tendence, zejména pak skepticismus vůči historické pravdě a subjektivismus, s kterým na historii nahlíží. The thesis examines the concept of the past in selected works by Julian Barnes, regarding the narrative techniques chosen by the author. It focuses on central thematic strands: fictionalizing of history and the bidirectional nature of past and present. The outlined thematic strands represent the author's approach to treating the theme of the past in his novels. Besides the common theme of the search for historical truth that Barnes' works share, the aim of this thesis is to examine the depiction of the past in relation to the themes of memory, truth, and how these factors shape the formation of individual and collective identities. The thesis considers not only narrative but also thematic inspiration provided to Barnes' contemporary writing by postmodern tendencies, particularly the scepticism towards historical truth and the subjectivism with which he views history. Keywords: Julian Barnes; minulost; historie; narativní technika; postmodernismus; pravda; paměť; identita Available in the Digital Repository of University of South Bohemia. Seizing the past in the Novels of Julian Barnes with Regard to the Author's Narrative Techniques

Diplomová práce zkoumá pojetí minulosti ve vybraných dílech Juliana Barnese, přičemž tento jev uvádí do souvislosti s narativními technikami, které autor zvolil. Práce se zaměřuje na ústřední ...

ČEŠKOVÁ, Karolína
Jihočeská univerzita v Českých Budějovicích, 2022

A case study comparing discursive strategies employed by a child at both pre and early school years as they seek to make sense of the world that surrounds them
ŠIMKOVÁ, Marie
2022 - English Tato diplomová práce si klade za cíl analyzovat narativní interakci mezi dítětem (Natálkou) a dospělou osobou s ohledem na diskurzivní strategie v kontextu představivosti a kreativity. Ve své práci jsem použila metodu konverzační analýzy, kterou jsem následně aplikovala na transkripční přepisy audio nahrávek, jež byly pořízeny během dvou časových obdobích: v roce 2018 a na přelomu roku 2020 při interakci mezi Natálkou a mnou. Analýza dat odhalila, že v repertoáru dítěte byly přítomny následující praktiky: (1) spojování dvou či více epizod ve vyprávění, (2) kladení otázek, (3) oddělování jednotlivých témat a (4) použití dalších diskurzivních elementů, konkrétně humoru, zveličování či nadsázky a vyjednávacích taktik. První část práce se podrobně věnuje diskurzivním praktikám a vysvětluje rozdíly mezi představivostí a kreativitou. Tato případová studie následně mapuje jakým způsobem se v průběhu dvou let vyvinulo použití zkoumaných strategií a charakterizuje, jak tyto změny reflektují kognitivní vývoj dítěte. The present thesis aims to analyse a narrative interaction between a child (Natálka) and an adult with regard to discursive strategies employed in the context of imagination and creativity. I applied the method of Conversation Analysis to the examination of the transcribed audio recordings that were taken in 2018 and at the turn of 2020 during the interaction between Natálka and myself. An analysis of the data identified that in the child's repertoire the following practices were present: (1) linking two or more episodes in a conversation, (2) asking questions, (3) separating individual topics, and (4) using other discourse elements; concretely humour, exaggeration, and negotiation techniques. The first part of the thesis sets out the discursive practices examined and argues for the insights these provide into the imagination and creativity of a child. The analysis, which is undertaken in the form of a case study, subsequently maps the use of the selected strategies developed over two years of Natálka's life and characterises how these changes reflect the child's cognitive development. Keywords: interakce; vývoj dětského narativu; diskurzivní praktiky; představivost a kreativita; kognitivní vývoj; konverzační analýza Available in the Digital Repository of University of South Bohemia. A case study comparing discursive strategies employed by a child at both pre and early school years as they seek to make sense of the world that surrounds them

Tato diplomová práce si klade za cíl analyzovat narativní interakci mezi dítětem (Natálkou) a dospělou osobou s ohledem na diskurzivní strategie v kontextu představivosti a kreativity. Ve své práci ...

ŠIMKOVÁ, Marie
Jihočeská univerzita v Českých Budějovicích, 2022

The Issue of Race in American Literature: A Comparative Study of Mark Twain's 19th-Century Novel The Adventures of Huckleberry Finn and Kathryn Stockett's Contemporary Work The Help
LANDOVÁ, Dominika
2022 - English Tato diplomová práce se zaměřuje na rasovou problematiku zobrazenou v americké literatuře. Porovnává díla Dobrodružství Huckleberryho Finna (1884) od Marka Twaina a Černobílý svět (2009) od Kathryn Stockett a popisuje, jak oba romány nahlížejí na roli Afroameričanů ve společnosti. Jelikož jsou díla zasazena do dvou nejvýznamnějších období v dějinách Spojených států amerických ve vztahu k rasové problematice, a to sice do období otroctví a rasové segregace, práce se také zabývá historickým kontextem, který je klíčový pro analýzu obou děl. Cílem práce je upozornit na podobnosti a odlišnosti románů týkajících se této problematiky. Autentičnost děl, zacházení s Afroameričany a pojetí jejich integrace do společnosti, kritika děl a další témata jsou rozebírána v rámci analýz. This diploma thesis focuses on the issue of race in American literature. It draws a comparison between Mark Twain's novel The Adventures of Huckleberry Finn (1884) and The Help (2009) by Kathryn Stockett and determines how both novels view the role of African Americans in society. Since these works depict two of the most significant periods in the history of the United States concerning the racial issue, namely the eras of slavery and racial segregation, the work also provides historical context of both periods, which is crucial for the analysis. The thesis aims to highlight the similarities and differences of the novels regarding this issue. The authenticity of these works, the treatment of African Americans and the concept of their integration into society, criticism, and other topics are discussed as part of the analyses. Keywords: Dobrodružství Huckleberryho Finna; Černobílý svět; otroctví; rasová segregace; rasová problematika; diskriminace; Afroameričané Available in the Digital Repository of University of South Bohemia. The Issue of Race in American Literature: A Comparative Study of Mark Twain's 19th-Century Novel The Adventures of Huckleberry Finn and Kathryn Stockett's Contemporary Work The Help

Tato diplomová práce se zaměřuje na rasovou problematiku zobrazenou v americké literatuře. Porovnává díla Dobrodružství Huckleberryho Finna (1884) od Marka Twaina a Černobílý svět (2009) od Kathryn ...

LANDOVÁ, Dominika
Jihočeská univerzita v Českých Budějovicích, 2022

Language Use and Management in International Organizations The experience of agents of the non-profit development agency sequa gGmbH
STOBBE, Sophia
2022 - English This master thesis aims to show what influence language and communication decisions have on external communication in internationally operating organizations. The research was done on the example of sequa gGmbH in Bonn, a non-profit development organization. Through interviews and observation protocols, the impressions and experiences of employees of sequa were collected. This master thesis aims to show what influence language and communication decisions have on external communication in internationally operating organizations. The research was done on the example of sequa gGmbH in Bonn, a non-profit development organization. Through interviews and observation protocols, the impressions and experiences of employees of sequa were collected. Keywords: language and communication decisions; intercultural communication; external communication; international organization; NGO. Available in the Digital Repository of University of South Bohemia. Language Use and Management in International Organizations The experience of agents of the non-profit development agency sequa gGmbH

This master thesis aims to show what influence language and communication decisions have on external communication in internationally operating organizations. The research was done on the example of ...

STOBBE, Sophia
Jihočeská univerzita v Českých Budějovicích, 2022

Social, economic, and environmental impact of high-speed rails in the Czech Republic: Expected impacts of high-speed rails on the Ústí nad Labem region
TOMS, Tomáš
2022 - English Uvedení prvních vysokorychlostních tratí do provozu ve druhé polovině 20. století nejen vdechlo nový život ustupující železnici, ale tento dopravní mód se stal páteří dopravy v mnoha vyspělých státech po celém světě. Mezi jeho největší výhody patří jak minimální dopad na životní prostředí, tak i související socio-ekonomické přínosy. Tato diplomová práce přibližuje problematiku vysokorychlostních tratí v severočeském Ústeckém kraji a zabývá se očekávanými dopady nové infrastruktury jak z pohledu obyvatel a cestujících, tak z pohledu expertů, kteří mají plánování a budování vysokorychlostních tratí v ČR na starosti. The commissioning of the first high-speed lines in the second half of the 20th century not only breathed new life into the receding railway, but this mode of transport has become the backbone of transport in many developed countries around the world. Among its greatest advantages are both the minimal environmental impact and the associated socio-economic benefits. This thesis introduces the issue of high-speed lines in the North Bohemian region of Ústí nad Labem and examines the expected impacts of the new infrastructure from the perspective of residents and passengers, as well as from the perspective of the experts in charge of planning and building high-speed lines in the country. Keywords: vysokorychlostní trať; VRT; Česká republika; Ústecký kraj; sociálně-ekonomický; environmentální; dopad Available in the Digital Repository of University of South Bohemia. Social, economic, and environmental impact of high-speed rails in the Czech Republic: Expected impacts of high-speed rails on the Ústí nad Labem region

Uvedení prvních vysokorychlostních tratí do provozu ve druhé polovině 20. století nejen vdechlo nový život ustupující železnici, ale tento dopravní mód se stal páteří dopravy v mnoha vyspělých státech ...

TOMS, Tomáš
Jihočeská univerzita v Českých Budějovicích, 2022

Digitization in the territory and differences between selected rural areas: the example of Grand Est region, France
PARMENT, Numa
2022 - English Available in the Digital Repository of University of South Bohemia. Digitization in the territory and differences between selected rural areas: the example of Grand Est region, France

PARMENT, Numa
Jihočeská univerzita v Českých Budějovicích, 2022

Multilingualism and Interculturality in international or interregional projects and work environments.
MICHELIN, Anais Marie Christelle
2022 - English The master thesis aimed to find out structural and individual strategies of managing multilingualism and interculturality within the BOKU - International Relations working team. In the first part of this work I set up a theoretical research framework focused on the following key topics : intercultural management, intercultural competence, intercultural teams, communication and challenges. The last part of the theoretical part describes the existing models about the University of Natural Resources and Life Sciences working environment I referred to. Empirical data were collected using data triangulation through document analysis and qualitative semi-structured interviews to demonstrate the structural and individual strategies of managing multilingualism and interculturality of six BOKU-IR team members. They were then analyzed by sequences and transcribed with the software FOLKER according to the convention GAT2. The results show an overview of the intercultural management and the strategies used to deal with multilingualism and interculturality within the BOKU- International Relational working environment. The master thesis aimed to find out structural and individual strategies of managing multilingualism and interculturality within the BOKU - International Relations working team. In the first part of this work I set up a theoretical research framework focused on the following key topics : intercultural management, intercultural competence, intercultural teams, communication and challenges. The last part of the theoretical part describes the existing models about the University of Natural Resources and Life Sciences working environment I referred to. Empirical data were collected using data triangulation through document analysis and qualitative semi-structured interviews to demonstrate the structural and individual strategies of managing multilingualism and interculturality of six BOKU-IR team members. They were then analyzed by sequences and transcribed with the software FOLKER according to the convention GAT2. The results show an overview of the intercultural management and the strategies used to deal with multilingualism and interculturality within the BOKU- International Relational working environment. Keywords: Multilingualism; interculturality; intercultural management; intercultural competences; intercultural communication; linguistic and cultural adaptations; team culture; intercultural and multilingual work environments; working together. Available in the Digital Repository of University of South Bohemia. Multilingualism and Interculturality in international or interregional projects and work environments.

The master thesis aimed to find out structural and individual strategies of managing multilingualism and interculturality within the BOKU - International Relations working team. In the first part of ...

MICHELIN, Anais Marie Christelle
Jihočeská univerzita v Českých Budějovicích, 2022

The challenges of multilingual and intercultural team collaboration. A case study of KAM organization.
BOSZCZYKOVÁ, Iva
2022 - English Tato diplomová práce se zabývá výzvami interkulturní a vícejazyčné týmové spolupráce na příkladu křesťanské neziskové organizace Křesťanská akademie mladých (KAM). Kvalitativní řízené rozhovory s jejími americkými pracovníky byly použity ke sběru dat, která poskytla informace o různých aspektech interkulturní a vícejazyčné týmové spolupráce, jako jsou výzvy, komunikace, vícejazyčnost a interkulturalita v organizaci. Následně byla k vyhodnocení dat použita kvalitativní obsahová analýza, která vedla k závěru, že jednou z největších výzev pro americké pracovníky této organizace je čeština. Z hlediska dlouhověkosti, kvality práce a života v České republice je nesmírně důležité naučit se český jazyk. This master thesis examines challenges of intercultural and multilingual team collaboration using the example of Christian non-profit organization - Křesťanská Akademie Mladých (KAM). Qualitative guided interviews with its American workers were used to collect data that provided information on various aspects of intercultural and multilingual team collaboration such as challenges, communication, multilingualism and interculturality in the organization. Subsequently, a qualitative content analysis was used to evaluate the data, which led to conclusion that one of the biggest challenges for the American staff of this organization is the Czech language. In terms of longevity, quality of work and life in the Czech Republic, it is extremely important to learn the Czech language. Keywords: týmová spolupráce; multikulturní týmy; interkulturalita; mnohojazyčnost; kultura; komunikace; výzvy; druhá kultura Available in the Digital Repository of University of South Bohemia. The challenges of multilingual and intercultural team collaboration. A case study of KAM organization.

Tato diplomová práce se zabývá výzvami interkulturní a vícejazyčné týmové spolupráce na příkladu křesťanské neziskové organizace Křesťanská akademie mladých (KAM). Kvalitativní řízené rozhovory s ...

BOSZCZYKOVÁ, Iva
Jihočeská univerzita v Českých Budějovicích, 2022