Number of found documents: 352
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\kur{Cryptosporidium myocastoris} n. sp. (Apicomplexa: Cryptosporidiidae), druh adaptovaný na nutrie (\kur{Myocastor coypus})
LIMPOUCHOVÁ, Zlata
2021 - Czech
Kryptosporidie jsou častí paraziti obratlovců. Tato práce popisuje nový druh kryptosporidií adaptovaný na nutrie. Celkem bylo vyšetřeno 150 vzorků trusu od zdivočelých nutrií v České republice a Slovensku. Vzorky byly vyšetřeny mikroskopicky a molekulárně na genech kódujících malou ribozomální podjednotku, aktin, HSP70 a gp60. Cryptosporidium spp., common parasite of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutria (Myocastor coypus). A total of 150 faecal samples of feral nutrias were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci. Keywords: kryptosporidie; nutrie; mikroskopická diagnostika; molekulární diagnostika; Cryptosporidium; nutria; microscopic diagnostic; molecular diagnostic Available in the Digital Repository of University of South Bohemia.
\kur{Cryptosporidium myocastoris} n. sp. (Apicomplexa: Cryptosporidiidae), druh adaptovaný na nutrie (\kur{Myocastor coypus})

Kryptosporidie jsou častí paraziti obratlovců. Tato práce popisuje nový druh kryptosporidií adaptovaný na nutrie. Celkem bylo vyšetřeno 150 vzorků trusu od zdivočelých nutrií v České republice a ...

LIMPOUCHOVÁ, Zlata
Jihočeská univerzita v Českých Budějovicích, 2021

Seasonality and weather conditions jointly drive flight activity patterns of aquatic and terrestrial chironomids
VEBROVÁ, Lucie
2021 - English
Dispersal by flight is crucial for long-term survival of organisms inhabiting dynamic environments, especially small standing waters. Surprisingly, flight activity of the most abundant macroinvertebrate group in standing freshwater habitats, the Chironomidae, has rarely been studied, leaving a gap in our understanding of the environmental drivers of their dispersal activity. The goal of this study was to investigate flight activity of adult chironomids on diel and seasonal timescales in sandpit pools in the Czech Republic. We show that aquatic and terrestrial chironomids use partly alternating time windows for their flight and partly differ in their responses to weather conditions. We also compare our results to previous studies that analysed the impact of weather conditions on flight activity of aquatic insects. Keywords: Aquatic insects; Dispersal; Seasonality; Environmental conditions; Collinearity; Chironomid adults Available in the Digital Repository of University of South Bohemia.
Seasonality and weather conditions jointly drive flight activity patterns of aquatic and terrestrial chironomids

Dispersal by flight is crucial for long-term survival of organisms inhabiting dynamic environments, especially small standing waters. Surprisingly, flight activity of the most abundant ...

VEBROVÁ, Lucie
Jihočeská univerzita v Českých Budějovicích, 2021

Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates
DUFKOVÁ, Kristýna
2021 - English
Urinary tract infections caused by Pseudomonas aeruginosa present a serious complication in the context of urinary catheter use. P. aeruginosa are equipped with various virulence determinants that may result in increased resistance. That contributes to higher morbidity and mortality in vulnerable patients. Pyocins are proteinaceous agents produced by pseudomonads with an antimicrobial effect targeting closely related bacteria. In this study, we examined the inhibition interactions among a set of 135 P. aeruginosa clinical isolates originated from the catheter-associated urinary tract infections. Togerther with pyocinogeny, the co-occurrence of other virulence factors and their co-association with pyocins were examined. The strong antagonistic interactions and high prevalence of pyocin genes among the isolates were observed. The overall competitive interactions provide promising findings in the area of alternative antibiotic approaches. Urinary tract infections caused by Pseudomonas aeruginosa present a serious complication in the context of urinary catheter use. P. aeruginosa are equipped with various virulence determinants that may result in increased resistance. That contributes to higher morbidity and mortality in vulnerable patients. Pyocins are proteinaceous agents produced by pseudomonads with an antimicrobial effect targeting closely related bacteria. In this study, we examined the inhibition interactions among a set of 135 P. aeruginosa clinical isolates originated from the catheter-associated urinary tract infections. Togerther with pyocinogeny, the co-occurrence of other virulence factors and their co-association with pyocins were examined. The strong antagonistic interactions and high prevalence of pyocin genes among the isolates were observed. The overall competitive interactions provide promising findings in the area of alternative antibiotic approaches. Keywords: bacteriocin; pyocin; Pseudomonas aeruginosa; CAUTI; siderophores Available in the Digital Repository of University of South Bohemia.
Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates

Urinary tract infections caused by Pseudomonas aeruginosa present a serious complication in the context of urinary catheter use. P. aeruginosa are equipped with various virulence determinants that may ...

DUFKOVÁ, Kristýna
Jihočeská univerzita v Českých Budějovicích, 2021

Habitat use of breeding birds in Central European reed beds
ŠŤASTNÝ, Vojtěch
2021 - English
The research dealing with habitat preferences of breeding birds in the northeast of Bohemia in the Czech Republic is based on the data on bird communities from 34 wetlands ranging in size from 0.76 to 70.42 ha, and relevant environmental factors, collected within the years 2016-2017. Habitat use among wetland bird species in relation to these factors has been compared and their effect on species diversity and the proportion of specially protected species according to the Act No. 114/1992 Coll. has been tested. Finally, management that may increase attractiveness of wetlands for birds was suggested. Keywords: bird community; diversity; habitat use; management; protected species; wetland Available in the Digital Repository of University of South Bohemia.
Habitat use of breeding birds in Central European reed beds

The research dealing with habitat preferences of breeding birds in the northeast of Bohemia in the Czech Republic is based on the data on bird communities from 34 wetlands ranging in size from 0.76 ...

ŠŤASTNÝ, Vojtěch
Jihočeská univerzita v Českých Budějovicích, 2021

Seed dispersal and realized gene flow of two forest orchids in a fragmented landscape
KOTILÍNEK, Milan
2021 - English
This thesis investigates the dispersal abilities of mycoheterotrophic plants represented by three orchid species. Seed trapping and gene flow study were used to examine this topic. This thesis investigates the dispersal abilities of mycoheterotrophic plants represented by three orchid species. Seed trapping and gene flow study were used to examine this topic. Keywords: Cephalanthera rubra; Epipactis atrorubens; fragmented landscape; gene flow; microsatellite DNA markers; seed dispersal; seed traps Available in the Digital Repository of University of South Bohemia.
Seed dispersal and realized gene flow of two forest orchids in a fragmented landscape

This thesis investigates the dispersal abilities of mycoheterotrophic plants represented by three orchid species. Seed trapping and gene flow study were used to examine this topic....

KOTILÍNEK, Milan
Jihočeská univerzita v Českých Budějovicích, 2021

Genome sequences of two Antarctic strains of Pseudomonas prosekii: insights into adaptation to extreme conditions
SNOPKOVÁ, Kateřina
2021 - Czech
Rod Pseudomonas obsahuje ubikvitní bakterie s vysokým biotechnologickým potenciálem. Nově popsaný druh Pseudomonas prosekii pochází ze vzorků získaných na ostrově Jamese Rosse v Antarktidě. Ačkoli lze předpokládat jeho endemismus, nebyly dosud popsány adaptační mechanismy zajišťující přežití baterie v extrémních podmínkách Antarktidy. Cílem páce bylo stanovit celogenomovou sekvenci dvou kmenů P. prosekii, genomy charakterizovat a dále se soustředit na geny související s adaptací na chlad a další stresové podmínky. V práci jsme identifikovali a analyzovali geny vázané na chladový šok a oxidační stres, osmoregulaci, produkci exopolysacharidů a sideroforů i geny související s tvorbu biofilmu. Srovnávací analýza P. prosekii a P. aeruginosa PAO1 vyzdvihla rozdíly v genomu extremofilního druhu a mezofilní oportunně patogenní bakterie. Pseudomonas is ubiquitous bacterial genus with the high biotechnological potential. Pseudomonas prosekii is species recently described from James Ross Island (Antarctica). Although its endemic lifestyle could be assumed, its low-temperature adaptation mechanisms have not yet been described and understood. Therefore, we sequenced two P. prosekii strains and performed deep genome analysis with a special respect to the genes related to cold adaptation. We identified and analyzed the genes responsible for cold shock and oxidative stress response, osmoregulation, exopolysaccharide and siderophores production, and biofilm formation. Comparative genome analysis of P. prosekii and P. aeruginosa PAO1 highlighted differences in genome content between extremophile species and a mesophilic opportunistic pathogen. Keywords: Pseudomonas prosekii; Antarktida; adaptace na chlad; analýza genomu; Pseudomonas prosekii; Antarctica; cold adaptation; genome-wide analysis Available in the Digital Repository of University of South Bohemia.
Genome sequences of two Antarctic strains of Pseudomonas prosekii: insights into adaptation to extreme conditions

Rod Pseudomonas obsahuje ubikvitní bakterie s vysokým biotechnologickým potenciálem. Nově popsaný druh Pseudomonas prosekii pochází ze vzorků získaných na ostrově Jamese Rosse v Antarktidě. Ačkoli lze ...

SNOPKOVÁ, Kateřina
Jihočeská univerzita v Českých Budějovicích, 2021

Diplomatické styky Československa a Svatého stolce v meziválečném období
ŠMÍD, Marek
2020 - Czech
Available in the Digital Repository of University of South Bohemia.
Diplomatické styky Československa a Svatého stolce v meziválečném období

ŠMÍD, Marek
Jihočeská univerzita v Českých Budějovicích, 2020

Ochrana člověka a společnosti - vývoj vzdělávání v bezpečnostních tématech
KAVAN, Štěpán
2020 - Czech
Available in the Digital Repository of University of South Bohemia.
Ochrana člověka a společnosti - vývoj vzdělávání v bezpečnostních tématech

KAVAN, Štěpán
Jihočeská univerzita v Českých Budějovicích, 2020

Totální syntéza [15N4] cytokininů a jejich využití
BUČEK, Jan
2020 - Czech
Rostlinné hormony cytokininy (CK), jejich metabolity a deriváty jsou jedny z esenciálních molekul ovlivňující celou řadu fyzilogických procesů rostlin jako je buněčné dělení, rostlinný růst a vývoj. Jejich stanovení a kvantifikace je náročné díky velmi malým konecntracím a komplikované rostlinné matrici. Z toho důvody je použití stabilně značených analogů nezbytné pro kvantifikaci, metabolické profilovaní a sledování biosyntetických drah. Tato práce představuje totální syntézu jedenácti [15N]-zančených C6-purinových derivátů, z toho pěti aromatických (4, 5, 6, 7, 8) a šesti isopreniodních (9, 10, 11) CK. Největší optimalizace, vzheldem k momentáně dostupným metodám, byla provedena v případě 6-amino-9H-[15N5]-purinu (adenin, 1) and 6-chloro-9H-[15N4]-purinu (6-chloropurin, 3). Poprvé je tak prezentován komplexní postup syntézy isotopově značených CKs z anorganického prekurzoru s vysokými výtěžky a chemickou čistotou. Identita připravených molekul byla v každém kroku ověřena pomocí řady fyzikálně chemických metod (TLC; HRMS; HPLC-MS; 1H, 13C, 15N NMR). Zde popsaný postup je v nepsolední řadě možno aplikovat na přípravu jakýchkoliv [15N4]-značených C6-substituovaných purinových derivátů. Cytokinins (CKs) and their metabolites and derivatives are essential for cell division, plant growth regulation and development. They are typically found at minute concentrations in plant tissues containing very complicated biological matrices. Therefore, defined standards labelled with stable isotopes are required for precise metabolic profiling and quantification of CKs, as well as in vivo elucidation of CK biosynthesis in various plant species. In this work, eleven [15N]-labelled C6-purine derivatives were prepared, among them five aromatic (4, 5, 6, 7, 8) and three isoprenoid (9, 10, 11) CKs. Compared to current methods, optimized syntheses of 6-amino-9H-[15N5]-purine (adenine, 1) and 6-chloro-9H-[15N4]-purine (6-chloropurine, 3) were performed to achieve more effective, selective and generally easier approaches. The chemical identity and purity of prepared compounds were confirmed by physico-chemical analyses (TLC; HRMS; HPLC-MS; 1H, 13C, 15N NMR). The presented approach is applicable for the synthesis of any other desired [15N4]-labelled C6-substituted purine derivatives. Keywords: cytokininy; [15N4]cytokininy; syntéza; isotopové značení; rostlinné hormony; puriny; cytokinins; [15N4]-cytokinins; synthesis; isotopic labelling; plant hormones; purnes Available in the Digital Repository of University of South Bohemia.
Totální syntéza [15N4] cytokininů a jejich využití

Rostlinné hormony cytokininy (CK), jejich metabolity a deriváty jsou jedny z esenciálních molekul ovlivňující celou řadu fyzilogických procesů rostlin jako je buněčné dělení, rostlinný růst a vývoj. ...

BUČEK, Jan
Jihočeská univerzita v Českých Budějovicích, 2020

Inovace výuky fyziky na studijním oboru obchodní akademie s ohledem na specifika oboru
JANTAČ, František
2020 - Czech
Available in the Digital Repository of University of South Bohemia.
Inovace výuky fyziky na studijním oboru obchodní akademie s ohledem na specifika oboru

JANTAČ, František
Jihočeská univerzita v Českých Budějovicích, 2020

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