Study of complexation of 5-phenylazopyrimidines with β-cyclodextrin by affinity capillary electrophoresis
Štěpánová, Sille; Procházková, Eliška; Hruzíková, Anna; Čechová, Lucie; Janeba, Zlatko; Kašička, Václav
2022 - English Keywords: affinity capillary electrophoresis; β-cyclodextrin; 5-phenylazopyrimidines Available at various institutes of the ASCR Study of complexation of 5-phenylazopyrimidines with β-cyclodextrin by affinity capillary electrophoresis

Štěpánová, Sille; Procházková, Eliška; Hruzíková, Anna; Čechová, Lucie; Janeba, Zlatko; Kašička, Václav
Ústav organické chemie a biochemie, 2022

Mercury Film Screen-Printed Electrodes
Josypčuk, Bohdan; Šestáková, Ivana; Tvorynska, Sofiia
2022 - English Mercury film screen-printed electrodes (MF-SPEs) were designed, prepared and tested for the first time as perspective representatives among SPEs that allow measuring at high negative potentials. For this purpose, the precise electrochemical (coulometric) preparation procedure for mercury deposition at the working electrode surface of the commercial silver-SPE was developed and a universal method of preparing MF-electrodes from different metal-SPEs was described. An optimal electrolyzer construction for the mercury deposition, electrolyte composition, electrolysis potential, and an activation of electrode surfaces were proposed. Determination of the zinc content in food supplement tablets was used for the MF-SPEs practical testing. Keywords: flow analysis; mercury film; screen-printed electrodes; electrochemistry Available on request at various institutes of the ASCR Mercury Film Screen-Printed Electrodes

Mercury film screen-printed electrodes (MF-SPEs) were designed, prepared and tested for the first time as perspective representatives among SPEs that allow measuring at high negative potentials. For ...

Josypčuk, Bohdan; Šestáková, Ivana; Tvorynska, Sofiia
Ústav fyzikální chemie J. Heyrovského, 2022

Spectroelectrochemistry of 1,10-phenantrolines Substituted by Pyrrolidine and Phenothiazine Redox-active Units
Sokolová, Romana; Wantulok, J.; Fiedler, Jan; Nycz, J.; Degano, I.
2022 - English Compounds based on 1,10-phenanthroline (Phen) and their complexes are used in many fields, such as a stabilizing agent in the synthesis of nanoparticles, catalysts in homogeneous catalysis and as a semiconductor in organic light-emitting diodes (OLED) due to their coordination abilities. In this work, 1,10-phenanthrolines functionalized by pyrrolidine and phenothiazine units were studied by electrochemical methods. Cyclic voltammetry resulted in several oxidation and reduction voltammetric peaks. Structure-activity relationship was investigated using in situ spectroeletrochemistry, spectrophotometry, infrared spectroscopy and chromatography. Keywords: 1,10-phenanthroline; Electrochemistry; Oxidation mechanism; Reduction mechanism Available on request at various institutes of the ASCR Spectroelectrochemistry of 1,10-phenantrolines Substituted by Pyrrolidine and Phenothiazine Redox-active Units

Compounds based on 1,10-phenanthroline (Phen) and their complexes are used in many fields, such as a stabilizing agent in the synthesis of nanoparticles, catalysts in homogeneous catalysis and as a ...

Sokolová, Romana; Wantulok, J.; Fiedler, Jan; Nycz, J.; Degano, I.
Ústav fyzikální chemie J. Heyrovského, 2022

Degradation of polycaprolactone electrospun materials - methods of analysis
Havlíčková, K.; Řezanka, M.; Kobera, Libor; Kuželová Košťáková, E.; Lukáš, D.; Jenčová, V.
2021 - English Biodegradable electrospun materials are widely used for medical application. Polycaprolatone is polymer suitable for electrospinning technology and is very often used to create nanofibrous scaffolds for tissue engineering. The time to disintegration or biodegradation of such materials is very important here. However, testing is not entirely easy. It is not possible to create exactly body-like conditions in vitro. Moreover, it is not easy to find suitable analytical methods that would show exactly what happens in the nanofibrous polycaprolactone electrospun samples at certain stages of degradation, ie how the internal structure of decaying nanofibers changes. This paper describes the traditional use of methods for testing polycaprolatone nanofibers by enzymatically catalysed degradation. Morphological changes are studied using scanning electron microscope images. However, it also offers a non-traditional analysis of polycaprolactone electrospun materials using the ssNMR method. Keywords: electrospinning; nanofibers; enzymatic degradation Available at various institutes of the ASCR Degradation of polycaprolactone electrospun materials - methods of analysis

Biodegradable electrospun materials are widely used for medical application. Polycaprolatone is polymer suitable for electrospinning technology and is very often used to create nanofibrous scaffolds ...

Havlíčková, K.; Řezanka, M.; Kobera, Libor; Kuželová Košťáková, E.; Lukáš, D.; Jenčová, V.
Ústav makromolekulární chemie, 2021

Toxic responses in human lung epithelial cells (BEAS-2B) exposed to particulate matter exhaust emissions from gasoline and biogasoline
Závodná, Táňa; Líbalová, Helena; Vrbová, Kristýna; Sikorová, Jitka; Vojtíšek-Lom, M.; Beránek, V.; Pechout, M.; Kléma, J.; Cigánek, M.; Machala, M.; Neča, J.; Rössner ml., Pavel; Topinka, Jan
2021 - English Motor vehicle emissions substantially contribute to air pollution worldwide and cause serious health problems. While the deleterious effects of diesel exhaust particulate matter (PM) have been widely studied, much less attention is paid to toxicity of PM emitted by gasoline engines although they also produce considerable amount of PM. The primary objective of this research was to assess toxic potencies of exhaust PM released by conventional gasoline engine fueled with neat gasoline (EU) or gasoline-ethanol blend (15% ethanol, v/v, E15). Despite a similar particle mass (mu g PM/kg fuel) produced by both fuels, PM emitted by E15 contained higher amount of harmful polycyclic aromatic hydrocarbons (PAH) as suggested by chemical analysis. To examine the toxicity of organic PM constituents, human lung BEAS-2B cells were exposed for 4h and 24h to a subtoxic dose of E0 and E15 PM organic extracts. We used genome scale transcriptomic analysis to characterize the toxic response and to identify modulated biological process and pathways. Whereas 4h exposure to both PM extracts resulted in modulation of similar genes and pathways related to lipid and steroid metabolism, activation of PPAR alpha, oxidative stress and immune response, 24h exposure was more specific for each extract, although both induced expression of PAH-metabolic enzymes, modulated metabolism of lipids or activated PPAR alpha, E15 additionally deregulated variety of other pathways. Overall, the PM mass produced by both fuels was similar, however, higher PAH content in E15 PM organic extract may have contributed to more extensive toxic response particularly after 24h exposure in BEAS-2B cells. Keywords: particulate matter emissions; gasoline; biofuels; toxicity; gene expression profiling Available at various institutes of the ASCR Toxic responses in human lung epithelial cells (BEAS-2B) exposed to particulate matter exhaust emissions from gasoline and biogasoline

Motor vehicle emissions substantially contribute to air pollution worldwide and cause serious health problems. While the deleterious effects of diesel exhaust particulate matter (PM) have been widely ...

Závodná, Táňa; Líbalová, Helena; Vrbová, Kristýna; Sikorová, Jitka; Vojtíšek-Lom, M.; Beránek, V.; Pechout, M.; Kléma, J.; Cigánek, M.; Machala, M.; Neča, J.; Rössner ml., Pavel; Topinka, Jan
Ústav experimentální medicíny, 2021

Looking into the Photo-stability of Eosin by Electrochemical Methods
Sabatini, F.; Sokolová, Romana; Degano, I.
2021 - English Eosin Y is a synthetic organic dye belonging to the xanthene family. Its bright and fluorescent\npink hue attracted and intrigued the painters of the 19th century, who experimented eosin as\npigment lake (often called Geranium lake) in their paintings. Nevertheless, eosin coloration is\nas beautiful as unstable, and examples of work of arts with faded hues have been documented.\nEosin is not only photo-sensitive but also pH sensitive, making its study very complex. In this\nwork, the combination of electrochemical and spectroscopic techniques revealed to be effective\nfor correlating the physical evidence of eosin fading with its chemical degradation. Keywords: Eosin; Synthetic dye; UV-Vis spectroelectrochemistry Available on request at various institutes of the ASCR Looking into the Photo-stability of Eosin by Electrochemical Methods

Eosin Y is a synthetic organic dye belonging to the xanthene family. Its bright and fluorescent\npink hue attracted and intrigued the painters of the 19th century, who experimented eosin as\npigment ...

Sabatini, F.; Sokolová, Romana; Degano, I.
Ústav fyzikální chemie J. Heyrovského, 2021

Oxidation Processes of N-methylguanines
Trnková, L.; Třísková, I.; Liška, Alan; Ludvík, Jiří
2021 - English The oxidation processes of N-methylguanines were investigated in both experimental and\ntheoretical way. Voltammetric oxidation potentials of guanine and its methyl analogues,\nacquired on polymer pencil graphite electrode (pPeGE), were compared with the HOMO\n(SOMO) energies calculated by density functional (DFT) methods. Our study contributes to\nunderstanding not only the oxidation processes of methylated guanines but also their role in\nepigenetics. Keywords: Guanine; N-methylated guanine; Oxidation process Available on request at various institutes of the ASCR Oxidation Processes of N-methylguanines

The oxidation processes of N-methylguanines were investigated in both experimental and\ntheoretical way. Voltammetric oxidation potentials of guanine and its methyl analogues,\nacquired on polymer ...

Trnková, L.; Třísková, I.; Liška, Alan; Ludvík, Jiří
Ústav fyzikální chemie J. Heyrovského, 2021

Integrated national-scale assessment of climate change impacts on agriculture: the case of the Czech Republic
Arbelaez Gaviria, Juliana; Boere, E.; Havlík, P.; Trnka, Miroslav
2021 - English In recent years, investigating climate change impacts in the agricultural sector at the national level has become a priority for adaptation decision-making. Most of these studies quantify the impacts\nof biophysical effects and often ignore the cross-sectoral interactions and economic effects on relative competitiveness, international trade, global food supply, and food prices for the Czech Republic. Ignoring future productivity changes globally under climate change scenarios can underestimate or overestimate climate change impacts at the national level. Here, we use GLOBIOM-CZE, a global economic model, as part of a climate change impact assessment framework to evaluate the impacts on the Czech agricultural sector in terms of environmental and economic indicators. By comparing with the baseline, the ensemble of scenarios suggests a decrease in crop area and production while increasing grassland, positively affecting livestock production by mid-century. Corn and barley show the most adverse response in production and area, while rapeseed increases under scenario RCP 8.5 with CO2 fertilization effect. Livestock products production is projected to increase, especially bovine meat and milk, as within RCP 8.5, no constraints are placed on growing greenhouse gas emissions. Keywords: climate change impacts; Czech agriculture; global assessment model Available at various institutes of the ASCR Integrated national-scale assessment of climate change impacts on agriculture: the case of the Czech Republic

In recent years, investigating climate change impacts in the agricultural sector at the national level has become a priority for adaptation decision-making. Most of these studies quantify the ...

Arbelaez Gaviria, Juliana; Boere, E.; Havlík, P.; Trnka, Miroslav
Ústav výzkumu globální změny , 2021

3rd CCP Phenogenomics Conference 2021: Abstract Book
Sedláček, Radislav
2021 - English The Conference was divided into two blocks. The first day was devoted to the theme “Human diseases and models“. The second day was specifically dedicated to preclinical development, including covid-19, which focused on translation of the basic research into the application. The Conference provided an excellent opportunity to support networking and interactions among the CCP users and experts. Keywords: phenogenomics; human diseases; mouse models; preclinical development; covid-19 Available at various institutes of the ASCR 3rd CCP Phenogenomics Conference 2021: Abstract Book

The Conference was divided into two blocks. The first day was devoted to the theme “Human diseases and models“. The second day was specifically dedicated to preclinical development, including ...

Sedláček, Radislav
Ústav molekulární genetiky, 2021

3D printed materials for the electrochemical determination of biologically active compounds
Choińska, Marta Katarzyna; Navrátil, Tomáš; Hrdlička, Vojtěch
2021 - English 3D printing materials are relatively novel materials in electrochemistry,\nused for manufacturing of electrochemical cells, electrodes\netc. They have been widely used mainly for tailored, fast, inexpensive,\nand easy preparation of various equipment for analyses of\nbiologically active compounds. Various medicinal products and illicit\ndrugs belong to this broad group of analytes which need to be\nmonitored because of the possibility of (un)intentional overdose or\nrecreational consumption, which can cause serious side effects or\neven death. Due to this risk, we decided to focus our research on the\ndevelopment of new, sensitive, selective, and easily customizable 3D\nprinted sensors for the determination of some commonly used\nantidepressants, analgesics, and illicit drugs. Keywords: analgesics; antidepressants; voltammetric determination Available on request at various institutes of the ASCR 3D printed materials for the electrochemical determination of biologically active compounds

3D printing materials are relatively novel materials in electrochemistry,\nused for manufacturing of electrochemical cells, electrodes\netc. They have been widely used mainly for tailored, fast, ...

Choińska, Marta Katarzyna; Navrátil, Tomáš; Hrdlička, Vojtěch
Ústav fyzikální chemie J. Heyrovského, 2021