No (286)
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SMV-2014-732: Preparation, testing and delivery of isoelectric point standards
Šlais, Karel
2014 - English
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously described aminomethylphenols. The prepared compounds are the good ampholytes with high hydrophility and molar absorbtivity.
Keywords:
Isoelectric focusing; low molecular mass pI markers; aminomethylphenols
Available on request at various institutes of the ASCR
SMV-2014-732: Preparation, testing and delivery of isoelectric point standards
The low molecular mass pI markers were prepared, examined and delivered with aim to fill up the previously described pI values. The pI markers comprise compounds based on our original previously ...
SMV-2014-706: Testování zařízení pro stanovení tritia a uhlíku 14C v ovzduší
Večeřa, Zbyněk
2014 - Czech
Testování a vývoj zařízení řady ”V3H14C” která jsou určena k odběru vzorků 3H a 14C ze vzduchu. Vzorky se zachycují do sorbentů, sorbentem pro 3H je standardně silikagel, sorbentem pro 14C je standardně hydroxid sodný. Získané vzorky jsou určeny k následné laboratorní analýze. Analýzou zjištěné hodnoty aktivit odebraných vzorků slouží ke stanovení bilance vypouštěného 3H a 14C z jaderných zařízení. Testing and development of the family”V3H14C” equipments designed for 3H and 14C sampling from the air. The samples are captured into sorbents, the standard sorbent for 3H is silica gel, and the standard sorbent for 14C is sodium hydroxide. The extracted samples are designed for subsequent laboratory analysis. Values of activity of samples, resulting from the analysis, serve for quantitative assessment of radioactive emissions of 3H and 14C from nuclear power plants e.g.
Keywords:
testing, air, radioactive emissions
Available on request at various institutes of the ASCR
SMV-2014-706: Testování zařízení pro stanovení tritia a uhlíku 14C v ovzduší
Testování a vývoj zařízení řady ”V3H14C” která jsou určena k odběru vzorků 3H a 14C ze vzduchu. Vzorky se zachycují do sorbentů, sorbentem pro 3H je standardně silikagel, sorbentem pro 14C je ...
Atomová absorpční spektrometrie
Dočekal, Bohumil
2014 - Czech
Atomová absorpční spektrometrie je jednou z nejrozšířenějších metod stopové prvkové analýzy, jednak pro vysokou citlivost stanovení, a jednak pro nízké pořizovací a provozní náklady. Umožňuje stanovení více než 60 prvků, všech kovových prvků a metaloidů, v koncentraci jednotek ug.l-1 až po několik desetin g.l-1. Text příspěvku popisuje základní principy metody, její instrumentace, a to od zdrojů záření, přes optické systémy až k moderním způsobům kompenzace nespecifické absorpce. Zabývá se systematicky jednotlivými technikami atomizace plamenové a bezplamenové. Rovněž jsou uvedeny analytické parametry metody, pracovní rozsahy, postupy standardizace, přístupy k metodologii a ke kontrole a zabezpečení kvality výsledků. Atomic absorption spectrometry is one of the most useful methods applied in trace element analysis, particularly due to high sensitivity and due to relatively low investment and operational costs. This method enables to determine up to 60 elements, metals and metalloids, within a wide concentration range from ug.l-1 to tenth of g.l-1. The contribution deals with fundamental principles of the method and instrumentation, including light sources, optical systems and modern background correction techniques. It systematically describes flame and flameless atomization and sample introduction techniques. Basic analytical parameters of the method, detection power, sensitivity, dynamic working range, standardization approaches, methodology, quality control and assurance are also discussed.
Keywords:
atomic absorption spectrometry; limit of detection; metal ions
Available on request at various institutes of the ASCR
Atomová absorpční spektrometrie
Atomová absorpční spektrometrie je jednou z nejrozšířenějších metod stopové prvkové analýzy, jednak pro vysokou citlivost stanovení, a jednak pro nízké pořizovací a provozní náklady. Umožňuje ...
Senzitive method of Caspase-3 detection in single stem cell
Adamová, Eva; Klepárník, Karel; Matalová, Eva
2013 - English
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Keywords:
caspase-3; single stem cell; miniaturization
Available on request at various institutes of the ASCR
Senzitive method of Caspase-3 detection in single stem cell
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods ...
Separation of tryptic digest of cytochrome C within a long nanoelectrospray tip
Týčová, Anna; Foret, František
2013 - English
Capillary electrophoresis coupled to mass spectrometry (CE-MS) posses powerful tool for separation and detection of wide range of ionic species. To avoid complicated constructions of interfaces we have conducted the separation within a long and thin nanoelectrospray tip. We have investigated main properties of such an experimental design on a complex sample of tryptic digest of cytochrome c.
Keywords:
cytochrome c; thin nanoelectrospray tip; electrophoretic separation
Available on request at various institutes of the ASCR
Separation of tryptic digest of cytochrome C within a long nanoelectrospray tip
Capillary electrophoresis coupled to mass spectrometry (CE-MS) posses powerful tool for separation and detection of wide range of ionic species. To avoid complicated constructions of interfaces we ...
New isoelectric focusing power supply based on features of voltage multiplier
Duša, Filip; Šlais, Karel
2013 - English
Present electrophoretic separation methods rely on sophisticated high-voltage power supplies capable of programing a voltage / current time course during an analysis. In this paper we suggest design of a simple highvoltage power supply for isoelectric focusing composed from affordable and commonly available electrical parts. It is based on features of the voltage multiplier invented by Cockcroft and Walton. Electrical characteristics of the power supply enabled power load controlled isoelectric focusing analysis thus eliminating need for programing voltage time course and reducing analysis total time.
Keywords:
isoelectric focusing; IEF device; electrodes
Available on request at various institutes of the ASCR
New isoelectric focusing power supply based on features of voltage multiplier
Present electrophoretic separation methods rely on sophisticated high-voltage power supplies capable of programing a voltage / current time course during an analysis. In this paper we suggest ...
Thin metal films for detection and preconcentration
Podešva, Pavel; Foret, František
2013 - English
We have tested CE separation of selected samples in capillaries with polydopamine modified surface. The capillaries were modified by polydopamine or polydopamine with an additive. The polydopamine coating with additive represents a simple and effective procedure for capillary alteration by another modificator such as hydroxyethyl cellulose, hydroxypropyl cellulose etc. In this work, we represent separation data from PrinCE system with UV detection for peptides, proteins and oligosaccharides labeled by 2-aminobenzoic acid.
Keywords:
thin metal film; chemoresistor; Au nanoparticles
Available on request at various institutes of the ASCR
Thin metal films for detection and preconcentration
We have tested CE separation of selected samples in capillaries with polydopamine modified surface. The capillaries were modified by polydopamine or polydopamine with an additive. The polydopamine ...
Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis
Vykydalová, Marie; Duša, Filip; Horká, Marie; Šlais, Karel
2013 - English
We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique.
Keywords:
bidirectional isotachophoresis; electrolyte system; preparative focusing
Available on request at various institutes of the ASCR
Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis
We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on ...
Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer
Lišková, Marcela; Klepárník, Karel; Pazdera, P.; Foret, František
2013 - English
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Förster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying.
Keywords:
caspase-3; Förster resonance energy transfer; apoptotic cells
Available on request at various institutes of the ASCR
Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences ...
Capillary isotachophoresis of anions with electrospray-ionization mass-spectrometric detection
Malá, Zdeňka; Gebauer, Petr; Boček, Petr
2013 - English
We present ITP-ESI-MS as a powerful tool for selective and sensitive analyses of anionic analytes. Our extended concept of ITP includes besides regular and freeacid ITP also self-maintained ITP in moving boundary systems. Easy theory describes the system properties including their stacking-window diagrams. Simple ESIcompatible electrolyte systems allow flexible tuning with very satisfactory results as demonstrated on the example of analysis of pharmaceuticals in waters.
Keywords:
capillary isotachophoresis; electrospray ionization; mass-spectrometric detection
Available on request at various institutes of the ASCR
Capillary isotachophoresis of anions with electrospray-ionization mass-spectrometric detection
We present ITP-ESI-MS as a powerful tool for selective and sensitive analyses of anionic analytes. Our extended concept of ITP includes besides regular and freeacid ITP also self-maintained ITP in ...
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