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Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch; Klepárník, Karel
2016 - English
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
Keywords:
single-cell analysis; apoptotic cells; non-apoptotic cells
Available in digital repository of the ASCR
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during ...
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
Lačná, J.; Foret, František; Kubáň, Petr
2016 - English
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new CE-LIF system. Then the derivatization process\nof MDA by TBA was optimized. The ability to use this method for the detection of\nMDA in a biological samples, such as EBC and plasma, was also demonstrated. For the\nfirst time it was possible to detect MDA in EBC samples by CE. Other biomarkers are\nnow being studied.
Keywords:
CE-LIF; malondialdehyde; biomarkers; lipids
Available in digital repository of the ASCR
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new ...
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Kubáň, Pavel; Boček, Petr; Seip, K. F.; Gjelstad, A.; Pedersen-Bjergaard, S.
2016 - English
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel approach requires μL volumes of samples and organic\nsolvents, which form consecutive immiscible plugs in a transparent polymeric capillary\nand the extraction process is accelerated by application of d.c. voltage. Number of the\nplugs, their composition, volume and sequence in the extraction capillary can be chosen\nspecifically for each particular application and high variability and selectivity of the\nextraction process can be achieved. In this contribution, we demonstrate suitability of\na five-phase μ-EME system for simultaneous extractions of cations and anions from\nurine and for selective extractions of basic analytes based on their acid-base strength\nfrom plasma.
Keywords:
micro-electromembrane extraction; sample pretreatment techniques; biological samples
Available in digital repository of the ASCR
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel ...
Characterization of FRET sensor
Datinská, Vladimíra; Klepárník, Karel; Belšánová, B.; Minárik, M.; Foret, František
2016 - English
In this study, we present characterization of sensor based on Fӧrster resonance energy\ntransfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory\nsynthesized quantum dot (QD). A complementary chain of a sample is labeled by a\nluminescent dye. When the dsDNA hybrid is formed, the energy from the QD (donor)\nis transferred to the dye (acceptor) and FRET is observed as a decrease of QD\nluminescence emission intensity and an increase of dye luminescence emission\nintensity.
Keywords:
FRET sensor; Fӧrster resonance energy transfer; quantum dot
Available in digital repository of the ASCR
Characterization of FRET sensor
In this study, we present characterization of sensor based on Fӧrster resonance energy\ntransfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory\nsynthesized quantum dot (QD). A ...
Microfluidic device for cell counting and characterization
Václavek, Tomáš; Křenková, Jana; Foret, František
2016 - English
Integration of cell counting unit is an important step in developing of microfluidic\ndevice for cellular analysis. Here we present a microfluidic device for cell counting\nbased on resistive pulse sensing.
Keywords:
microfluidic devices; microfluidic chip; cellular analysis
Available in digital repository of the ASCR
Microfluidic device for cell counting and characterization
Integration of cell counting unit is an important step in developing of microfluidic\ndevice for cellular analysis. Here we present a microfluidic device for cell counting\nbased on resistive pulse ...
Organic markers and compounds in PM1 aerosol in small town near Prague (Czech Republic) in winter 2016
Křůmal, Kamil; Mikuška, Pavel; Coufalík, Pavel; Večeřa, Zbyněk
2016 - English
Contribution summarizes the concentrations of monosaccharide anhydrides, resin\nacids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes,\nhopanes, steranes and polycyclic aromatic hydrocarbons in PM1 aerosols in small town\nKladno-Švermov in winter. According to concentrations of organic markers, the\nidentification of emission sources in Kladno-Švermov is discussed.
Keywords:
organic markers; PM1 aerosol; combustion of biomass; aerosols collected
Available in digital repository of the ASCR
Organic markers and compounds in PM1 aerosol in small town near Prague (Czech Republic) in winter 2016
Contribution summarizes the concentrations of monosaccharide anhydrides, resin\nacids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes,\nhopanes, steranes and polycyclic ...
Capillary electrophoresis with contactless conductivity detection for rapid determination of meldonium in urine
Šlampová, Andrea; Kubáň, Pavel
2016 - English
A simple, rapid and cheap capillary electrophoresis-capacitively coupled contactless\nconductivity detection (CE-C4D) method for determination of meldonium (MEL) in\nurine samples was developed. Sample pretreatment was minimized to dilution of urine\nsamples with DI water and MEL was determined by CE-C4D in background electrolyte\nconsisting of 2 M acetic acid (pH 2.3) in ng mL−1 to mg mL−1 concentration range.\nSample-to-sample analysis time was less than 4 min, limit of detection and\nquantification was 0.015 and 0.05 μg mL−1, respectively, and the method showed\nexcellent linearity (r2 ≥ 0.9998), recovery (97.6–99.9%) and intraday as well as interday\nrepeatability (RSD ≤ 4.7%).
Keywords:
meldonium; capillary electrophoresis-capacitively coupled contactless conductivity detection; urine
Available in digital repository of the ASCR
Capillary electrophoresis with contactless conductivity detection for rapid determination of meldonium in urine
A simple, rapid and cheap capillary electrophoresis-capacitively coupled contactless\nconductivity detection (CE-C4D) method for determination of meldonium (MEL) in\nurine samples was developed. ...
Prototyping of a simple device for parallel dot blotting using CNC machining
Novotný, Jakub; Svobodová, Z.; Bílková, Z.; Foret, František
2016 - English
Computer numerical control (CNC) machining has been tested for rapid prototyping of\na new design of a dot-blotting device suitable for parallel testing of antibodies. The\ndevice, machined from polycarbonate, was prepared using an inexpensive computer\ncontrolled CNC router.
Keywords:
CNC machines; dot-blotters; CNC router
Available in digital repository of the ASCR
Prototyping of a simple device for parallel dot blotting using CNC machining
Computer numerical control (CNC) machining has been tested for rapid prototyping of\na new design of a dot-blotting device suitable for parallel testing of antibodies. The\ndevice, machined from ...
Evaluation of stationary phases for HPLC based on nanofibers
Čmelík, Richard; Foret, František
2016 - English
In this study, the performance parameters were compared for the set of chromatographic columns filled with polyacrylonitrile nanofibers processed in the different manner. The advantages and challenges of the novel fillings were discussed.
Keywords:
nanofiber; HPLC; stationary phase
Available in digital repository of the ASCR
Evaluation of stationary phases for HPLC based on nanofibers
In this study, the performance parameters were compared for the set of chromatographic columns filled with polyacrylonitrile nanofibers processed in the different manner. The advantages and challenges ...
Geochemistry of Cenozoic volcanic rocks of the Faroe Islands with a focus on their ultra-trace contents of Hg
Šišková, P.; Krmíček, Lukáš; Coufalík, Pavel
2016 - English
According to geophysical research performed in the eastern part of the North Atlantic, many authors inferred that the basalt flows on the Faroe Islands overlie a continental metamorphic basement supposedly of Precambrian age. With respect to this, we focused our study on determination of ultratrace mercury contents by means of atomic absorption spectrometry to test this hypothesis. Mercury contents in basalts seem to be a sensitive tool for distinguishing between the basalts not affected by interaction with the continental crust and the basalts enclosing crustal xenoliths. Contents of Hg show great variability between samples even within the same formation. We presume that high concentrations of Hg in some samples are related to stagnation of individual pulses of parent magma in the continental crust below the Faroe Islands.
Keywords:
Faroe Islands; tholeiitic basalt; mercury content; continental crust
Available in digital repository of the ASCR
Geochemistry of Cenozoic volcanic rocks of the Faroe Islands with a focus on their ultra-trace contents of Hg
According to geophysical research performed in the eastern part of the North Atlantic, many authors inferred that the basalt flows on the Faroe Islands overlie a continental metamorphic basement ...
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