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Partial deletion of the RPS0A-binding domain of TIF32/eIF3A affects 40S ribosome-association of the multifactor complex and severely impairs reinitiation on the GCN4 mRNA leader
Szamecz, Bela; Rutkai, Edit; Nielsen, K. H.; Valášek, Leoš
2006 - English
Yeast Initiation factor 3 (eIF3) occurs together with the eIF2.GTP.Met-tRNAiMet ternary complex (TC) and eIFs 1 and 5 in a pre-formed unit designated the multifactor complex (MFC) that was implicated in playing a critical role in ecient recruitment of TC and mRNA to the 40S ribosomes and stimulation of the post-assembly processes such as scanning and AUG recognition. In ecort to identify binding sites of the MFC on the 40S ribosome, we previously demonstrated that deletion of the rst 199 amino acids of the N-terminal domain (NTD) of the TIF32 subunit of eIF3/eIF3a, in the presence of a wild-type gene, completely eliminated association of the mutant MFC with the 40S ribosome without aecting its overall integrity. In addition, we showed that the TIF32-NTD contains a binding site for the C-terminal domain (CTD) of the small ribosomal protein 0A (RPS0A) that is located on the solvent side of the 40S subunit where the main body of eIF3 was proposed to reside Práce pojednáva o studiu inciace translace a specifické úloze translačního iniciačního faktoru eIF3
Keywords:
translation; gcn4
Available at various institutes of the ASCR
Partial deletion of the RPS0A-binding domain of TIF32/eIF3A affects 40S ribosome-association of the multifactor complex and severely impairs reinitiation on the GCN4 mRNA leader
Yeast Initiation factor 3 (eIF3) occurs together with the eIF2.GTP.Met-tRNAiMet ternary complex (TC) and eIFs 1 and 5 in a pre-formed unit designated the multifactor complex (MFC) that was implicated ...
Cryptic secondary metabolite biosynthetic clusters in Streptomyces ambofaciens
Nezbedová, Šárka; Karray, F.; Juguet, M.; Aigle, B.; Pernodet, J. L.; Weiser, Jaroslav
2006 - English
We looked for conditions leading to activation of silent gene clusters implied from the sequence as responsible for biosynthesis of yet unknown secondary metabolites in mutants blocked in biosynthesis of known antibiotics (spiramycin, congocidin). This approach should contribute to the better understanding of interaction between primary and secondary metabolism during production of antibiotics and the role of morphological differentiation in these processes U série mutant blokovaných v biosyntetických dráhách známých antibiotik (spiramycin, congocidin) jsme hledali podmínky pro aktivaci přítomných „mlčících“ souborů genů pro biosyntézu zatím nedefinovaných sekundárních metabolitů. Cílem je přispět k lepšímu pochopení principů interakce mezi primárním a sekundárním metabolizmem během produkce antibiotik a objasnit úlohu morfologické diferenciace v těchto procesech
Keywords:
streptomyces ambofaciens; metabolism
Available at various institutes of the ASCR
Cryptic secondary metabolite biosynthetic clusters in Streptomyces ambofaciens
We looked for conditions leading to activation of silent gene clusters implied from the sequence as responsible for biosynthesis of yet unknown secondary metabolites in mutants blocked in biosynthesis ...
Interaction of Apomyoglobin with Lipid Vesicles Probed by H/D Exchange
Man, Petr; Montagner, C.; Dublet, B.; Yelmo, Ch. B.; Halada, Petr; Forge, V.; Forest, E.
2006 - English
Interaction between apomyoglobin and lipidic vesicles was investigated by hydrogen deuterium exchange coupled to mass spectrometry. We revealed two regions in the protein implicated in the pH dependent binding to the surface of lipidic membrane Interakce mezi apomyoglobinem a lipidosomy byla studována pomocí izotopové výměny a hmotnostní spektrometrie. Byly identifikovány dvě oblasti ve struktuře proteinu zapojené do vazby na lipidovou membránu
Keywords:
mass spectrometry; apomyoglobin
Available at various institutes of the ASCR
Interaction of Apomyoglobin with Lipid Vesicles Probed by H/D Exchange
Interaction between apomyoglobin and lipidic vesicles was investigated by hydrogen deuterium exchange coupled to mass spectrometry. We revealed two regions in the protein implicated in the pH ...
Gliadin induce the activation of human peripheral blood monocytes
Cinová, Jana; Palová-Jelínková, Lenka; Smythies, L.; Černá, M.; Pecharová, Barbara; Dvořák, M.; Fruhauf, P.; Tlaskalová, Helena; Smith, P.; Tučková, Ludmila
2006 - English
We documented that gliadin digest stimulate PBMoC (isolated from celiac patients and healthydonors) to IL-8 and TNF alfa production through the NF-kappa B pathway. Monocytes expressed increased levels of dendritic cell markers U monocytů izolovaných z periferní krve celiakálních pacientů a zdravých dárců jsme zjistili rozdíly v produkci IL-8 a TNF alfa, aktivační dráha vede přes NF-kappa B systém
Keywords:
monocytes; gliadin fragments; dendritic markers
Available at various institutes of the ASCR
Gliadin induce the activation of human peripheral blood monocytes
We documented that gliadin digest stimulate PBMoC (isolated from celiac patients and healthydonors) to IL-8 and TNF alfa production through the NF-kappa B pathway. Monocytes expressed increased levels ...
Oral administration of live probiotic bacteria influences the severity of experimental acute colitis in Balb/c mice
Kokešová, Alena; Sokol, Dan; Bártová, J.; Rossmann, Pavel; Tlaskalová, Helena
2006 - English
Our data extend the evidence that non-pathogenic luminal bacteria participate in the pathogenesis of chronic intestinal inflammation. Administration of probiotic bacteria influencing the composition of intestinal flora may be relevant to the development of novel strategies in the prevention and management of IBD patiens Výsledky studie přinášejí doklady o účasti nepatogenních střevních bakterií u patogeneze chronického střevního zánětu. Podání probiotických bakterií ovlivňuje složení střevní flory a má význam pro vývoj nových strategií v prevenci a léčbě nemocných se střevními záněty
Keywords:
chronic intestinal inflammation; non-pathogenic bacteria; probiotic bacteria
Available at various institutes of the ASCR
Oral administration of live probiotic bacteria influences the severity of experimental acute colitis in Balb/c mice
Our data extend the evidence that non-pathogenic luminal bacteria participate in the pathogenesis of chronic intestinal inflammation. Administration of probiotic bacteria influencing the composition ...
Targeted polymeric anticancer drugs based on HPMA in therapy of HNSCC
Bouček, Jan; Betka, J.; Strohalm, Jiří; Plocová, Daniela; Šubr, Vladimír; Mrkvan, Tomáš; Ulbrich, Karel; Říhová, Blanka
2006 - English
Targeted conjugates are promising novel drugs and especially anti-EGFR targeted conjugates seem to be a perspective modality in treating HNSCC or other cancers over-expressing EGFR receptor Směrovaná proléčiva a zejména konjugáty směrované proti EBFR jsou slibnou perspektivou pro léčbu nádoru hlavy a krku
Keywords:
head carcinoma; neck carcinoma
Available at various institutes of the ASCR
Targeted polymeric anticancer drugs based on HPMA in therapy of HNSCC
Targeted conjugates are promising novel drugs and especially anti-EGFR targeted conjugates seem to be a perspective modality in treating HNSCC or other cancers over-expressing EGFR ...
Purification and characterization of penicillin G acylase expressed in Escherichia coli and Pichia pastoris
Marková, Zdenka; Marešová, Helena; Kyslík, Pavel
2006 - English
The pga gene coding for penicillin G acylase from strain E. coli RE3 was cloned on pPICZA plasmid and expressed in the yeast Pichia pastoris X33. The enzyme was purified from both strains and further characterized Gen kódující penicilin-G-acylasu z produkčního kmene E. coli RE3 byl klonován do plazmidu pPICZA a exprimován v Pichia pastoris X33. Z obou hostitelských kmenů E. coli a P. pastoris byla penicilin-G-acylasa izolována a charakterizovány její vlastnosti
Keywords:
purification; characterization
Available at various institutes of the ASCR
Purification and characterization of penicillin G acylase expressed in Escherichia coli and Pichia pastoris
The pga gene coding for penicillin G acylase from strain E. coli RE3 was cloned on pPICZA plasmid and expressed in the yeast Pichia pastoris X33. The enzyme was purified from both strains and further ...
Influence of stringent response in Corynebacterium glutamicum on valine production
Prouza, Marek; Holátko, Jiří; Elišáková, Veronika; Nešvera, Jan; Pátek, Miroslav
2006 - English
The aim of this study was to compare the production of valine in C. glutamicum producers with the level of valine produced by the strain with deletion of the rel gene coding for enzyme guanosine tetraphosphate synthetase Cílem práce bylo porovnat tvorbu valinu u producentů C. glutamicum s tvorbou valinu u kmene s delecí genu rel, který kóduje enzym guanosintetrafosfátsynthetasu
Keywords:
corynebacterium glutamicum; guanosine tetraphosphate synthetase
Available at various institutes of the ASCR
Influence of stringent response in Corynebacterium glutamicum on valine production
The aim of this study was to compare the production of valine in C. glutamicum producers with the level of valine produced by the strain with deletion of the rel gene coding for enzyme guanosine ...
Stimulation effects of gliadin to human dendritic cells
Pecharová, Barbara; Palová-Jelínková, Lenka; Rožková, D.; Bártová, J.; Šedivá, A.; Tlaskalová, Helena; Spíšek, R.; Tučková, Ludmila
2006 - English
This work studies the stimulatins effects of gliadin to human dendritic cells Tato práce se zabývá výzkumem stimulačního efektu gliadinu na lidské dendritické buňky
Keywords:
dendritic cells; antigen; gliadin peptides
Available at various institutes of the ASCR
Stimulation effects of gliadin to human dendritic cells
This work studies the stimulatins effects of gliadin to human dendritic cells
Tato práce se zabývá výzkumem stimulačního efektu gliadinu na lidské dendritické buňky
Investigation of genes involved in phenol and naphthalene degradation in Rhodococcus erythropolis CCM2595 and Rhodococcus sp. NCIMB12038
Knoppová, Monika; Kulakov, L. A.; Larkin, M. J.; Pátek, Miroslav
2006 - English
The strain R. erythropolis CCM2595 is able to degrade phenol by the beta-ketoadipate pathway and the strain Rhodococcus sp. NCIMB12038 is able to degrade naphthalene via gentisate. Enzyme studies of these strains indicate that strain NCIMB12038 also contains enzymes necessary for phenol utilization and strain CCM2595 can also degrade naphthalene. Pulse field gel electrophoresis was used and southern hybridisation experiments were performed to reveal localization the genes involved in phenol and naphthalene degradation in both strains Kmen R. erythropolis CCM2595 se vyznačuje schopností degradovat fenol keto-adipátovou dráhou a kmen Rhodococcus sp. NCIMB12038 je schopen degradovat naftalen dráhou gentisátovou. Enzymové studie těchto kmenů ukazují, že kmen NCIMB12038 nese také geny nezbytné pro degradaci fenolu a kmen CCM2595 také pro degradaci naftalenu. Pulsní gelovou elektroforézou a southern hybridizací jsme lokalizovali geny podílející se na degradaci fenolu a naftalenu v obou kmenech
Keywords:
phenol; rhodococcus erythropolis
Available at various institutes of the ASCR
Investigation of genes involved in phenol and naphthalene degradation in Rhodococcus erythropolis CCM2595 and Rhodococcus sp. NCIMB12038
The strain R. erythropolis CCM2595 is able to degrade phenol by the beta-ketoadipate pathway and the strain Rhodococcus sp. NCIMB12038 is able to degrade naphthalene via gentisate. Enzyme studies of ...
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