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In depth study of physiological roles of TIF34/eIF3i and TIF35/eIF3g subunits of yeast
Janoušková, Olga; Nielsen, K. H.; Valášek, Leoš
2006 - English
Translation initiation is a multiple-step process involving a large number of soluble eukaryotic initiation factors that ultimately leads to formation of the 80S ribosome with Met-tRNAiMet based paired with the start AUG codon of mRNA. Among the initiation factors, eIF3 draws a special attention since it was shown to promote several critical steps such as recruitment of the eIF2GTPMet-tRNAiMet ternary complex and mRNA to the 40S ribosomal subunit and was implicated in cancer Práce pojednáva o studiu inciace translace a specifické úloze translačního iniciačního faktoru eIF3
Keywords:
eukaryotic translation; yeast
Available at various institutes of the ASCR
In depth study of physiological roles of TIF34/eIF3i and TIF35/eIF3g subunits of yeast
Translation initiation is a multiple-step process involving a large number of soluble eukaryotic initiation factors that ultimately leads to formation of the 80S ribosome with Met-tRNAiMet based ...
New built bioreactor control systems for Bioengineering equipment
Prell, Aleš; Sobotka, Miroslav; Kujan, Petr; Šafář, Hynek; Holler, Pavel
2006 - English
In cooperation of the automation company Proteco Pardubice the complete replacement of classic analogue control units of next two bioreactors by new, PLC driven local control systems for all monitored and controlled parameters was done. Some measuring devices and actuators were substituted by a new technique too and, moreover, a new central SCADA/HMI (Reliance, Geovap Pardubice) system of the set of bioreactors was introduced and the remaining analogue driven fermentors were integrated into it Ve spolupráci s automatizační společností Proteco Pardubice byla provedena kompletní výměna analogových řídících jednotek bioreaktorů a vybudovány nové lokální a centrální řídící systémy založené na PLC Tecomat a SCADA/HMI Reliance, Geovap Pardubice. Zbývající analogové řídící systémy byly integrovány
Keywords:
fermenter control; monitoring
Available at various institutes of the ASCR
New built bioreactor control systems for Bioengineering equipment
In cooperation of the automation company Proteco Pardubice the complete replacement of classic analogue control units of next two bioreactors by new, PLC driven local control systems for all monitored ...
Secondary metabolites in insect associated Geosmithia species: polyhydroxylated anthraquinones of G. lavendula and perspectives
Kolařík, Miroslav; Stodůlková, Eva; Šulc, Miroslav; Kuzma, Marek; Man, Petr; Flieger, Miroslav; Křesinová, Zdena
2006 - English
Cultures of G. lavendula produce various colored hydroxylated antraquinones (AQ). Rhodolamprometrin and pentahydroxy-AQ were identified so far. Natural AQs are used as dyes and pharmaceuticals Kultury G. lavendula produkují řadu barevných hydroxylovaných antrachinonů (AQ). Dosud jsme identifikovali rhodolamprometrin and pentahydroxy-AQ. Přírodní AQ jsou využívány jako barviva a léčiva
Keywords:
geosmithia lavendula; hydroxylated antraquinones
Available at various institutes of the ASCR
Secondary metabolites in insect associated Geosmithia species: polyhydroxylated anthraquinones of G. lavendula and perspectives
Cultures of G. lavendula produce various colored hydroxylated antraquinones (AQ). Rhodolamprometrin and pentahydroxy-AQ were identified so far. Natural AQs are used as dyes and ...
The effect of isoleucine limitation on valine precursor levels and acetohydroxyacid synthase activity in stringent (rel+) and relaxed (rel-) Corynebacterium glutamicum mutant
Denina, I.; Paegle, L.; Ruklisha, M.; Prouza, Marek; Pátek, Miroslav
2006 - English
Isoleucine limitation was found to increase acetohydroxyacid synthase activity in Corynebacterium glutamicum cells. Similar increase of activity of this enzyme was observed in C. glutamicum rel+ strains as well in rel- mutant Bylo zjištěno, že limitace isoleucinem vede ke zvýšení aktivity synthasy acetohydroxykyselin v buňkách Corynebacterium glutamicum. Podobné zvýšení aktivity tohoto enzymu bylo pozorováno u kmene C. glutamicum rel+ i u mutanty rel-
Keywords:
isoleucine; corynebacterium glutamicum
Available at various institutes of the ASCR
The effect of isoleucine limitation on valine precursor levels and acetohydroxyacid synthase activity in stringent (rel+) and relaxed (rel-) Corynebacterium glutamicum mutant
Isoleucine limitation was found to increase acetohydroxyacid synthase activity in Corynebacterium glutamicum cells. Similar increase of activity of this enzyme was observed in C. glutamicum rel+ ...
The Influence of Calcium to Calmodulin-Melittin Complex: A study Using Chemical Gross-linking and FTMS
Giannakopulos, A.; Derrick, P. J.; Havlíček, Vladimír; Novák, Petr
2006 - English
Melittin binds tightly to calmodulin in the presence of calcium and inhibits the function o f calmodulin. Chemical cross-linking combined with mass spectrometry has been used in order to define the positions of Calmodulin and melittin in the complex at different concentrations of calcium Melittin se váže pevně kalmodulin v přítomnosti vápníku a inhibuje jeho funkci. Chemické zesítění a hmotnostní spektrometrie byly použity za účlem nalezení místa intrakce mezi kalmodulinem a melittin za ruzných koncentrací vápníku
Keywords:
mass spectrometry; chemical cross-linking
Available at various institutes of the ASCR
The Influence of Calcium to Calmodulin-Melittin Complex: A study Using Chemical Gross-linking and FTMS
Melittin binds tightly to calmodulin in the presence of calcium and inhibits the function o f calmodulin. Chemical cross-linking combined with mass spectrometry has been used in order to define the ...
Evaluation of interactions between lectin-like receptors using glycodendrimers
Vannucci, Luca; Pospíšil, Miloslav; Rossmann, Pavel; Chernyavskiy, Oleksandr; Kubínová, Lucie; Křen, Vladimír; Luptovcová, Martina; Svoboda, Jan; Kuldová, Markéta; Bezouška, Karel; Fišerová, Anna
2006 - English
The events following the administration of GN8 in vitro, in vivo and ex vivo, lead to increased expression of CD69. The CD69-dependent IFNg production is modulated by co-activation of NKG2D and Ly49 receptors. Glycodendrimers can assist the dissection of activation network of NK/NKT cell lectin-like receptors in rat and murine models Podání GN8 v in vitro, in vivo a ex vivo podmínkách zvyšuje expresi CD69 antigenu. CD69-dependentní produkce IFNg je ovlivněna rovněž aktivací NKG2D a Ly49 receptorů. Glykodendrimery jsou vhodné pro studium NK/NKT buněčné aktivační sítě lektinových receptorů v potkaních a myších modelech
Keywords:
glycodendrimers; lectin-like receptors; cytotoxic lymphocytes
Available at various institutes of the ASCR
Evaluation of interactions between lectin-like receptors using glycodendrimers
The events following the administration of GN8 in vitro, in vivo and ex vivo, lead to increased expression of CD69. The CD69-dependent IFNg production is modulated by co-activation of NKG2D and Ly49 ...
Toll-like receptors -2, -4 and CD14 in human intestinal mucosa from patients with inflammatory Bowel Disease (IBD)
Frolová, Lenka; Drastich, P.; Klimešová, Klára; Rossmann, Pavel; Tlaskalová, Helena
2006 - English
This study show that IBD may be associated with increased expression of TLR2 and CD14 in the intestinal epithelium of the patients Studie ukazuje, že NSZ mohou být spojeny se zvýšenou expresí TLR2 and CD14 ve střevním epitelu pacientů
Keywords:
crohns disease; ulcerative colitis; toll-like receptors
Available at various institutes of the ASCR
Toll-like receptors -2, -4 and CD14 in human intestinal mucosa from patients with inflammatory Bowel Disease (IBD)
This study show that IBD may be associated with increased expression of TLR2 and CD14 in the intestinal epithelium of the patients...
Morphological changes during azoxymethane and dextran sodium sulfate-induced colon carcinogenesis in mice model
Klimešová, Klára; Rossmann, Pavel; Kverka, Miloslav; Frolová, Lenka; Tlaskalová, Helena
2006 - English
This study confirmed an important role of inflammation in colon carcinogenesis. Developing of tumors is usually closely associated with the areas of inflammation Studie potvrdila významnou úlohu zánětu při karcinogeneze a ukázala obrazy počáteční dysplasie a časná stadia nádoru
Keywords:
colorectal cancer; balb/c mice; dextran sodium sulfate
Available at various institutes of the ASCR
Morphological changes during azoxymethane and dextran sodium sulfate-induced colon carcinogenesis in mice model
This study confirmed an important role of inflammation in colon carcinogenesis. Developing of tumors is usually closely associated with the areas of inflammation...
Targeted polymer-bound drugs for cancer therapy
Kovář, Marek; Mrkvan, Tomáš; Šírová, Milada; Strohalm, Jiří; Šubr, Vladimír; Etrych, Tomáš; Ulbrich, Karel; Říhová, Blanka
2006 - English
We have presented data on development of polymer-bound cytostatic drugs and their therapeutic efficacy in selected tumor models Byly prezentovány výsledky výzkumu zaměřeného na vývoj polymerních systémů jakožto nosiče cytostatik, a účinnost takovýchto konjugátů byla demonstrována na zvolených nádorových modelech
Keywords:
polymer-bound drugs; tumor; cytostatic
Available at various institutes of the ASCR
Targeted polymer-bound drugs for cancer therapy
We have presented data on development of polymer-bound cytostatic drugs and their therapeutic efficacy in selected tumor models...
Heat shock proteins can have disease suppressive therapeutic effects in inflammatory bowel disease models
Van Eden, W.; Van der Zee, R.; Kverka, Miloslav; Hauet-Broere, F.; Pieters, R. H. H.; Bol-Schoenmakers, M. W. H. C.; Tlaskalová, Helena
2006 - English
This work studies implication of the heat shock proteins and their suppresive effects in IBD models Práce se zabývá studiem proteinů tepelného šoku a jejich významu pro terapeutický efekt v modelech střevních zánětů
Keywords:
heat shock proteins; inflammatory bowel disease; peptides
Available at various institutes of the ASCR
Heat shock proteins can have disease suppressive therapeutic effects in inflammatory bowel disease models
This work studies implication of the heat shock proteins and their suppresive effects in IBD models...
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