Number of found documents: 293
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Scale-up and production of various recombinant oxidoreductases
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2017 - English
Development and optimization of pilot cultivations and DSP of recombinant intracellular oxidoreductases in microbial productions for a food industrial use Keywords: enzyme production Available at various institutes of the ASCR
Scale-up and production of various recombinant oxidoreductases

Development and optimization of pilot cultivations and DSP of recombinant intracellular oxidoreductases in microbial productions for a food industrial use

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
Mikrobiologický ústav, 2017

UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
Kotrbáček, V.; Doucha, Jiří; Kuchařová, V.; Doubek, J.
2017 - English
We studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass significantly increased content of carotenoids in egg yolks of 8 experimental animals already after the first week of supplementation (P<0,01). Compared to 8 control hens these differences remained significantly higher until the end of the eight-week experiment. The average carotenoid concentration was 17,3 mu g per g of yolk in control group and 25,3 mu g per g of yolk in experimental animals (P<0,01). Autotrophically grown Chlorella was supplemented to the 30 gilts one month prior the expected first heat in a dose of 1,5 g per animal per day. These sows entered heat earlier and the number of pregnancy was higher in comparison to the 30 control animals. Other studies observed digestibility of selenium organically bound in Se-enriched Chlorella. The level of selenium in blood, colostrum and also tissues of new born piglets was determined. Group of 8 sows was supplemented with Se-enriched alga during the pregnancy and 3 days after parturition at a dose of 0,3 mg of selenium per 1 kg of feed. The same amount of selenium in the form of sodium selenite was administrated to the control group of 8 individuals. Organic form of selenium significantly increased concentration of Se in blood of sows and colostrum (P<0,05). Piglets born to supplemented sows had also significantly higher level of Se in heart, muscles and liver (P<0.01). The use of fresh Chlorella biomass produced outdoors on a dairy farm was experimentally tested. The liquid fresh biomass applied to dairy cows during the pregnancy at a dose of 15 g dry mass per cow increased the dry matter content in colostrum from 17,5 g per 100 ml of colostrum in control group to 20,5 g per 100 ml of colostrum in supplemented cows (P<0,05). Keywords: Chlorella; feed supplement; flue gas utilization Available at various institutes of the ASCR
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES

We studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass ...

Kotrbáček, V.; Doucha, Jiří; Kuchařová, V.; Doubek, J.
Mikrobiologický ústav, 2017

Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2017 - English
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up. Keywords: pastoris production Available at various institutes of the ASCR
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up

Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
Mikrobiologický ústav, 2017

Pilot scale production of a dietary supplement.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
Pilot scale cultivations of biotransformation enzymes producer, the biotransformation of the dietary supplement and its DSP. Keywords: dietary supplements; fungal fermentation Available at various institutes of the ASCR
Pilot scale production of a dietary supplement.

Pilot scale cultivations of biotransformation enzymes producer, the biotransformation of the dietary supplement and its DSP.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
Mikrobiologický ústav, 2016

Development of the anaerobic submerged cultivation.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
Development of the anaerobic cultivation of bacteria with a specific lipopolysaccharide content in the cell structures. Keywords: anaerobic cultivation Available at various institutes of the ASCR
Development of the anaerobic submerged cultivation.

Development of the anaerobic cultivation of bacteria with a specific lipopolysaccharide content in the cell structures.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
Mikrobiologický ústav, 2016

Development of pilot production technology of phosphorylases.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
Development and optimization of the production of phosporylases for pharmaceutical industry. Cultivation technology and down-stream processing. Keywords: enzyme production Available at various institutes of the ASCR
Development of pilot production technology of phosphorylases.

Development and optimization of the production of phosporylases for pharmaceutical industry. Cultivation technology and down-stream processing.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
Mikrobiologický ústav, 2016

ANTIBACTERIAL EFFECT OF SELECTED NANOPARTICLES AS REVEALED BY DOUBLING TIME OF TREATED ANTHOMONAS CAMESTRIS PV. CAMPESTRIS CULTURES
Pečenka, J.; Svobodová, Kateřina; Eichmeier, A.; Baránek, M.
2016 - English
Besides many possibilities of applications of nanoparticles in the field of medicine, diagnostics, molecular biology, bioorganic chemistry or remediation of environment, there is also a potential of employment of nanoparticles as a tool for elimination and control of bacteria invading plant tissue. In this experiment an antibacterial activity of selected nanoparticles based on silver, gold and bimetallic silver/copper was tested on bacteria Xanthomonas campestris pv. campestris (Xcc) (strain 1279a). The strongest inhibitory effect represented by doubling time of treated cultures was measured in the presence of the smallest silver nanoparticles (9 nm) at the highest concentration (5 ppm). Keywords: nanoparticles; Xanthomonas campestris; doubling time Available at various institutes of the ASCR
ANTIBACTERIAL EFFECT OF SELECTED NANOPARTICLES AS REVEALED BY DOUBLING TIME OF TREATED ANTHOMONAS CAMESTRIS PV. CAMPESTRIS CULTURES

Besides many possibilities of applications of nanoparticles in the field of medicine, diagnostics, molecular biology, bioorganic chemistry or remediation of environment, there is also a potential of ...

Pečenka, J.; Svobodová, Kateřina; Eichmeier, A.; Baránek, M.
Mikrobiologický ústav, 2016

Cultivation and DSP of the Pseudomonas bacteria to produce low molecular natural compounds.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
The set-up of the pilot cultivation of Pseudomonas spp. bacteria to produce specific low molecular natural compounds for pharmaceutical purposes. Keywords: natural substances production Available at various institutes of the ASCR
Cultivation and DSP of the Pseudomonas bacteria to produce low molecular natural compounds.

The set-up of the pilot cultivation of Pseudomonas spp. bacteria to produce specific low molecular natural compounds for pharmaceutical purposes.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
Mikrobiologický ústav, 2016

Optimization of media for cultivation of the bacterium E. coli with specific proteins in the cell wall.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - English
Optimization of media for preparation of bacterial culture for isolation of specific proteins. Keywords: microbial production Available at various institutes of the ASCR
Optimization of media for cultivation of the bacterium E. coli with specific proteins in the cell wall.

Optimization of media for preparation of bacterial culture for isolation of specific proteins.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
Mikrobiologický ústav, 2016

Optimization of pilot production technology of lactamases and development of Pichia pastoris technology
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2015 - English
Development and optimization of the production of lactamases for pharmaceutical industry. Cultivation technology and down-stream processing. Development of the cultivation and DSP technology of the yeast Pichia pastoris in the pilot scale. Keywords: Pichia cultivation Available at various institutes of the ASCR
Optimization of pilot production technology of lactamases and development of Pichia pastoris technology

Development and optimization of the production of lactamases for pharmaceutical industry. Cultivation technology and down-stream processing. Development of the cultivation and DSP technology of the ...

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
Mikrobiologický ústav, 2015

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