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Circadian rhythms of photosynthesis in marine diatom Thalassiosira weissflogii under nitrogen replete and deplete conditions
Kasalický, Vojtěch; Rottnerová, K.; Sciandra, A.; Babin, M.; Prášil, Ondřej
2007 - English
T. weissflogii was grown under dynamic irradiance conditions (sinusoidal light-dark regime) and then switched to constant, low irradiance. Cellular characteristics (pigment and nutrient content, cell size) indicated synchronization of algal population, furthermore pronounced variations in the aktivity of variable fluorescence, thermoluminescence and in the gene expression (rbcL, psbA, fcp) were observed T. weissflogii byla pěstována v podmínkách dynamické ozářenosti (sinusoidní režim) a poté přenesa na konstantní světlo o nízké intenzitě. Buněčné charakteristiky (obsah pigmentů a nutrientů, velikost buněk) indikovaly synchronizaci řasové populace, dále byly pozorovány výrazné změny v aktivitě variabilní fluorescence, termoluminiscence a v expresi genů (rbcL, psbA, fcp)
Keywords:
circadian rhythms
Available at various institutes of the ASCR
Circadian rhythms of photosynthesis in marine diatom Thalassiosira weissflogii under nitrogen replete and deplete conditions
T. weissflogii was grown under dynamic irradiance conditions (sinusoidal light-dark regime) and then switched to constant, low irradiance. Cellular characteristics (pigment and nutrient content, cell ...
Increased CO2 enhances nitrogen fixation and growth in the marine cyanobacterium Trichodesmium IMS101
Levitan, O.; Prášil, Ondřej; Berman-Frank, I.
2007 - English
Anthropogenic increases in atmospheric pCO2 over the last century are accompanied by higher concentrations of CO2 (aq) in the surface oceans. These changes could influence aquatic primary production including cyanobacterial nitrogen-fixers via the coupling between photosynthesis and nitrogen-fixation. We hypothesized that elevated pCO2 levels may enhance nitrogen fixation and photosynthesis in the bloom-forming, diazotrophic, cyanobacterium Trichodesmium spp. that contributes greatly to primary production in the oligotrophic subtropical and tropical oceans. We acclimated cultures of Trichodesmium IMS101 to 3 pCO2s (pre-industrial -250 ppmv, current - 400, future -900 ppmv) and examined changes in morphology, photosynthesis, nitrogen fixation, and growth. High pCO2 enhanced growth, filament length and nitrogen fixation of Trichodesmium in comparison with both ambient and low pCO2 acclimated cultures Antropogenní zvýšení atmosférického CO2 může ovlivnit i mořské diazotrofní sinice, které fixují atmosferický uhlík. Ukázali jsme, že zvýšení CO2 má pozitivní vliv na rychlost růstu a fixace dusíku u sinice Trichodesmium. Pravděpodobným mechanismem je alokace energie a reduktantů od mechanismů koncentrace uhlíku v buňkách k mechanismům fixace dusíku
Keywords:
nirogen fixation; global change; trichodesmium
Available at various institutes of the ASCR
Increased CO2 enhances nitrogen fixation and growth in the marine cyanobacterium Trichodesmium IMS101
Anthropogenic increases in atmospheric pCO2 over the last century are accompanied by higher concentrations of CO2 (aq) in the surface oceans. These changes could influence aquatic primary production ...
Differences in development of colorectal carcinoma in conventional and germ-free mice
Klimešová, Klára; Rossmann, Pavel; Kverka, Miloslav; Frolová, Lenka; Tlaskalová, Helena
2007 - English
This study helps in determination of the role of intestinal microflora in colon carcinogenesis Pomocí této studie chceme lépe určit roly mikroflory při vzniku kolorektálního karcinomu a navrhnout způsoby, kterými lze tento proces ovlivnit
Keywords:
colorectal
Available at various institutes of the ASCR
Differences in development of colorectal carcinoma in conventional and germ-free mice
This study helps in determination of the role of intestinal microflora in colon carcinogenesis...
Immunologically active components of human milk and their changes after pasteurization
Burianová, Jaroslava; Kverka, Miloslav; Hofmanová, Běla; Houška, M.; Měřička, P.; Tlaskalová, Helena
2007 - English
In our study milk pasteurization (both heat and pressure one) was shown to cause changes of immunologically active components V naší studii jsme prokázali, že úprava mateřského mléka teplem nebo tlakem před podáním novorozencům ovlivňuje aktivitu imunologických složek
Keywords:
human milk
Available at various institutes of the ASCR
Immunologically active components of human milk and their changes after pasteurization
In our study milk pasteurization (both heat and pressure one) was shown to cause changes of immunologically active components...
Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31)
Weiserová, Marie; Ryu, J.
2007 - English
We have characterised a putative restriction-modification system EcoA0ORF42P in commensal Escherichia coli strain A0 34/86 (O83: K24: H31). This system is a functional member of the Type IB family, whose specificity differs from those of known Type IB enzymes, as proved by immunological cross-reactivity and complementation assay. Using a plasmid transformation method and RM search computer program, we have identified the DNA recognition sequence of EcoA0ORF42P as GGA(8N)ATGC Charakterizovali jsme předpokládaný restrikčně-modifikační systém EcoA0ORF42P komensálního kmene Escherichia coli A0 34/86 (O83:K24:H31). Imunologická analýza a komplementační testy prokázaly, že se jedná o funkční systém patřící do skupiny Typu IB, jehož specifita se liší od dosud známých enzymů této skupiny. Byla identifikovaná specifická sekvence na DNA GGA(8N)ATGC rozpoznávaná enzymem EcoA0ORF42P
Keywords:
escherichia coli
Available at various institutes of the ASCR
Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31)
We have characterised a putative restriction-modification system EcoA0ORF42P in commensal Escherichia coli strain A0 34/86 (O83: K24: H31). This system is a functional member of the Type IB family, ...
Targeted HPMA copolymer-bound doxorubicin conjugates in the treatment of established tumors and induction of effective anti-tumor immunity
Kovář, Marek; Tomala, Jakub; Mrkvan, Tomáš; Etrych, Tomáš; Strohalm, Jiří; Ulbrich, Karel; Říhová, Blanka
2007 - English
Thus, treatment with B1 mAb-targeted conjugates is able to completely cured BCL 1 bearing mice and most importantly, break immunoescaping mechanisms of BCL1 cells Léčba BCL1 leukemie pomocí anti-idiotopově směrovaného konjugátu na bázi poly(HPMA) obsahujícím doxorubicin je schopna kompletně vyléčit experimentální zvířata. Navíc u kompletně vyléčených zvířat dochází k navození BCL1-specifické protinádorové imunity
Keywords:
hpma
Available at various institutes of the ASCR
Targeted HPMA copolymer-bound doxorubicin conjugates in the treatment of established tumors and induction of effective anti-tumor immunity
Thus, treatment with B1 mAb-targeted conjugates is able to completely cured BCL 1 bearing mice and most importantly, break immunoescaping mechanisms of BCL1 cells...
HPMA-based polymeric conjugate of doxorubicin acting in concert with the host anti-tumour immunity
Šírová, Milada; Rossmann, Pavel; Mrkvan, Tomáš; Strohalm, Jiří; Šubr, Vladimír; Plocová, Daniela; Ulbrich, Karel; Říhová, Blanka
2007 - English
Polymeric conjugate Dox-HPMA-HuIg directly targets the tumour due to the EPR effect, it is not detrimental towards the immune system of the host, and acts in concert with the anti-tumour immune responses Polymerní konjugát Dox-HPMA-HuIg působí přímo proti nádoru prostřednictvím EPR efektu, nepoškozuje imunitní systém nositele a působí ve shodě s jeho imunitní odpovědí
Keywords:
doxorubicin
Available at various institutes of the ASCR
HPMA-based polymeric conjugate of doxorubicin acting in concert with the host anti-tumour immunity
Polymeric conjugate Dox-HPMA-HuIg directly targets the tumour due to the EPR effect, it is not detrimental towards the immune system of the host, and acts in concert with the anti-tumour immune ...
Degradation of humic acids by letter-decomposing basidiomycetes
Šnajdr, Jaroslav; Trögl, Josef; Steffen, K.; Heeg, K.; Hofrichter, M.; Baldrian, Petr
2007 - English
Two strains of saprotrophic basidiomycetes - Hypholoma fasciculare and Rhodocollybia butyracea – were isolated from the oak wood in Xaverov (Prague, Czech Republic). We followed the production of extracellular enzymes in a laboratory setup as well as the composition of microbial community during the colonization of reconstructed soil profile Dva kmeny saprotrofních basidiomycet - Hypholoma fasciculare a Rhodocollybia butyracea - byly izolovány z dubového lesa v Xaverově (Praha). V laboratorním experimentu jsme sledovali produkci extracelulárních enzymů a složení mikrobiálního společenstva v průběhu kolonizace rekonstruovaného půdního profilu
Keywords:
saprotrophic basidiomycetes
Available at various institutes of the ASCR
Degradation of humic acids by letter-decomposing basidiomycetes
Two strains of saprotrophic basidiomycetes - Hypholoma fasciculare and Rhodocollybia butyracea – were isolated from the oak wood in Xaverov (Prague, Czech Republic). We followed the production of ...
Surface membrane glycosylation of EL-4 cells: the impact of free or N-(2-hydroxypropyl)methacrylamide (HPMA) bound doxorubicin
Kovář, Lubomír; Etrych, Tomáš; Šubr, Vladimír; Strohalm, Jiří; Sklenář, Jan; Chytil, Petr; Ulbrich, Karel; Říhová, Blanka
2007 - English
In those experimenst we studied the possible impace of CD43 as highly glycosylated molecule in cells exposed to free doxorubicin (Dox) or to conjugates consisting of HPMA copolymer backbone to which the drug is conjugated via enzymatically (PK1 conjugate) or pH sensitive, hydrolytically cleavable bond (hydrazone conjugate, HYD). V těchto pokusech jsme sledovali vliv vysoce glykosylované povrchové molekuly CD43 u buněk vystaveným volnému doxorubicinu nebo doxorubicinu konjugovanému k HPMA polymeru
Keywords:
doxorubicin
Available at various institutes of the ASCR
Surface membrane glycosylation of EL-4 cells: the impact of free or N-(2-hydroxypropyl)methacrylamide (HPMA) bound doxorubicin
In those experimenst we studied the possible impace of CD43 as highly glycosylated molecule in cells exposed to free doxorubicin (Dox) or to conjugates consisting of HPMA copolymer backbone to which ...
Fluorescence kinetic microscopy: 2D imaging of variable chlorophyll fluorescence from single algal cells with spectral resolution
Prášil, Ondřej; Šetlíková, Eva; Veselka, D.
2007 - English
The fluorescence kinetic microscopy (FKM) provides new dimension in application of chlorophyll fluorescence techniques. It combines classical fluorescence microscopy with 2D kinetic detection systems. FKM allows to examine photosynthesis of individual algal cells or on the cellular level of macroscopic structures. FKM is well suited to study heterogeneity in photosynthetic response among individual cells in algal cultures or in natural mixed populations. Special microscopic chambers allow to maintain live cells under optimal conditions for several hours or days and to follow evolution of photosynthesis in individual cells during the course of their growth cycle. Recent technical developments have allowed to determine FKM fluorescence kinetic with spectral resolution Předmětem workshopu je seznámení se s technikou fluorescenční kinetické spektroskopie s 2D rozlišením, pro charakterizaci fotosyntetické aktivity jednotlivých buněk řas a sinic
Keywords:
fluorescence; kinetic; microscopy
Available at various institutes of the ASCR
Fluorescence kinetic microscopy: 2D imaging of variable chlorophyll fluorescence from single algal cells with spectral resolution
The fluorescence kinetic microscopy (FKM) provides new dimension in application of chlorophyll fluorescence techniques. It combines classical fluorescence microscopy with 2D kinetic detection systems. ...
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