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Aktivity of thioredoxin reduktase in wild and seleniumresistant strains of the alga Scenedesmus quadricauda grown in the presence of inorganic selenium compounds
Vítová, Milada; Umysová, Dáša; Doušková, Irena; Rucki, M.; Zachleder, Vilém
2008 - English
We have selected resistant strains of Scenedesmus quadricauda able to tolerate high selenium concentration up to 400 mg/L added either as selenate Na2SeO4 (strain SeVI) or selenite Na2SeO3 (strain SeIV). We have investigated activity of selenoprotein thioredoxin reductase (TR) in both wild type and Se-resistant strains. Concomitantly, a total amount of organically bound selenium was evaluated in biomass dry weight. We have found that activity of TR rapidly increases in the SeVI resistant strain in the presence of selenate, while no effect on TR activity was found in SeIV resistant strain and only slight increase in wild type in the presence of selenite Vyselektovali jsme rezistentní kmeny Scenedesmus quadricauda schopné tolerovat vysoké koncentrace selenu až do 400 mg/L přidávaného buď ve formě selenanu Na2SeO4 (kmen SeVI) nebo seleničitanu Na2SeO3 (kmen SeIV). Studovali jsme aktivitu selenoproteinu thioredoxin reduktázy (TR) jak u divokého tak u rezistentních kmenů. Zároveň jsme stanovovali celkové množství organicky vázaného selenu v sušině. Zjistili jsme, že aktivita TR se rapidně zvyšuje u SeVI rezistentního kmene v přítomnosti selenanu, zatímco u SeIV rezistentního kmene jsme nezaznamenali žádný efekt a pouze mírné zvýšení aktivity u divokého kmene v přítomnosti seleničitanu
Keywords:
selenium; alga; scenedesmus quadricauda
Available at various institutes of the ASCR
Aktivity of thioredoxin reduktase in wild and seleniumresistant strains of the alga Scenedesmus quadricauda grown in the presence of inorganic selenium compounds
We have selected resistant strains of Scenedesmus quadricauda able to tolerate high selenium concentration up to 400 mg/L added either as selenate Na2SeO4 (strain SeVI) or selenite Na2SeO3 (strain ...
Circadian rhythms of photosynthesis in marine diatom Thalassiosira weissflogii under nitrogen replete and deplete conditions
Kasalický, Vojtěch; Rottnerová, K.; Sciandra, A.; Babin, M.; Prášil, Ondřej
2007 - English
T. weissflogii was grown under dynamic irradiance conditions (sinusoidal light-dark regime) and then switched to constant, low irradiance. Cellular characteristics (pigment and nutrient content, cell size) indicated synchronization of algal population, furthermore pronounced variations in the aktivity of variable fluorescence, thermoluminescence and in the gene expression (rbcL, psbA, fcp) were observed T. weissflogii byla pěstována v podmínkách dynamické ozářenosti (sinusoidní režim) a poté přenesa na konstantní světlo o nízké intenzitě. Buněčné charakteristiky (obsah pigmentů a nutrientů, velikost buněk) indikovaly synchronizaci řasové populace, dále byly pozorovány výrazné změny v aktivitě variabilní fluorescence, termoluminiscence a v expresi genů (rbcL, psbA, fcp)
Keywords:
circadian rhythms
Available at various institutes of the ASCR
Circadian rhythms of photosynthesis in marine diatom Thalassiosira weissflogii under nitrogen replete and deplete conditions
T. weissflogii was grown under dynamic irradiance conditions (sinusoidal light-dark regime) and then switched to constant, low irradiance. Cellular characteristics (pigment and nutrient content, cell ...
Increased CO2 enhances nitrogen fixation and growth in the marine cyanobacterium Trichodesmium IMS101
Levitan, O.; Prášil, Ondřej; Berman-Frank, I.
2007 - English
Anthropogenic increases in atmospheric pCO2 over the last century are accompanied by higher concentrations of CO2 (aq) in the surface oceans. These changes could influence aquatic primary production including cyanobacterial nitrogen-fixers via the coupling between photosynthesis and nitrogen-fixation. We hypothesized that elevated pCO2 levels may enhance nitrogen fixation and photosynthesis in the bloom-forming, diazotrophic, cyanobacterium Trichodesmium spp. that contributes greatly to primary production in the oligotrophic subtropical and tropical oceans. We acclimated cultures of Trichodesmium IMS101 to 3 pCO2s (pre-industrial -250 ppmv, current - 400, future -900 ppmv) and examined changes in morphology, photosynthesis, nitrogen fixation, and growth. High pCO2 enhanced growth, filament length and nitrogen fixation of Trichodesmium in comparison with both ambient and low pCO2 acclimated cultures Antropogenní zvýšení atmosférického CO2 může ovlivnit i mořské diazotrofní sinice, které fixují atmosferický uhlík. Ukázali jsme, že zvýšení CO2 má pozitivní vliv na rychlost růstu a fixace dusíku u sinice Trichodesmium. Pravděpodobným mechanismem je alokace energie a reduktantů od mechanismů koncentrace uhlíku v buňkách k mechanismům fixace dusíku
Keywords:
nirogen fixation; global change; trichodesmium
Available at various institutes of the ASCR
Increased CO2 enhances nitrogen fixation and growth in the marine cyanobacterium Trichodesmium IMS101
Anthropogenic increases in atmospheric pCO2 over the last century are accompanied by higher concentrations of CO2 (aq) in the surface oceans. These changes could influence aquatic primary production ...
Differences in development of colorectal carcinoma in conventional and germ-free mice
Klimešová, Klára; Rossmann, Pavel; Kverka, Miloslav; Frolová, Lenka; Tlaskalová, Helena
2007 - English
This study helps in determination of the role of intestinal microflora in colon carcinogenesis Pomocí této studie chceme lépe určit roly mikroflory při vzniku kolorektálního karcinomu a navrhnout způsoby, kterými lze tento proces ovlivnit
Keywords:
colorectal
Available at various institutes of the ASCR
Differences in development of colorectal carcinoma in conventional and germ-free mice
This study helps in determination of the role of intestinal microflora in colon carcinogenesis...
Immunologically active components of human milk and their changes after pasteurization
Burianová, Jaroslava; Kverka, Miloslav; Hofmanová, Běla; Houška, M.; Měřička, P.; Tlaskalová, Helena
2007 - English
In our study milk pasteurization (both heat and pressure one) was shown to cause changes of immunologically active components V naší studii jsme prokázali, že úprava mateřského mléka teplem nebo tlakem před podáním novorozencům ovlivňuje aktivitu imunologických složek
Keywords:
human milk
Available at various institutes of the ASCR
Immunologically active components of human milk and their changes after pasteurization
In our study milk pasteurization (both heat and pressure one) was shown to cause changes of immunologically active components...
Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31)
Weiserová, Marie; Ryu, J.
2007 - English
We have characterised a putative restriction-modification system EcoA0ORF42P in commensal Escherichia coli strain A0 34/86 (O83: K24: H31). This system is a functional member of the Type IB family, whose specificity differs from those of known Type IB enzymes, as proved by immunological cross-reactivity and complementation assay. Using a plasmid transformation method and RM search computer program, we have identified the DNA recognition sequence of EcoA0ORF42P as GGA(8N)ATGC Charakterizovali jsme předpokládaný restrikčně-modifikační systém EcoA0ORF42P komensálního kmene Escherichia coli A0 34/86 (O83:K24:H31). Imunologická analýza a komplementační testy prokázaly, že se jedná o funkční systém patřící do skupiny Typu IB, jehož specifita se liší od dosud známých enzymů této skupiny. Byla identifikovaná specifická sekvence na DNA GGA(8N)ATGC rozpoznávaná enzymem EcoA0ORF42P
Keywords:
escherichia coli
Available at various institutes of the ASCR
Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31)
We have characterised a putative restriction-modification system EcoA0ORF42P in commensal Escherichia coli strain A0 34/86 (O83: K24: H31). This system is a functional member of the Type IB family, ...
Targeted HPMA copolymer-bound doxorubicin conjugates in the treatment of established tumors and induction of effective anti-tumor immunity
Kovář, Marek; Tomala, Jakub; Mrkvan, Tomáš; Etrych, Tomáš; Strohalm, Jiří; Ulbrich, Karel; Říhová, Blanka
2007 - English
Thus, treatment with B1 mAb-targeted conjugates is able to completely cured BCL 1 bearing mice and most importantly, break immunoescaping mechanisms of BCL1 cells Léčba BCL1 leukemie pomocí anti-idiotopově směrovaného konjugátu na bázi poly(HPMA) obsahujícím doxorubicin je schopna kompletně vyléčit experimentální zvířata. Navíc u kompletně vyléčených zvířat dochází k navození BCL1-specifické protinádorové imunity
Keywords:
hpma
Available at various institutes of the ASCR
Targeted HPMA copolymer-bound doxorubicin conjugates in the treatment of established tumors and induction of effective anti-tumor immunity
Thus, treatment with B1 mAb-targeted conjugates is able to completely cured BCL 1 bearing mice and most importantly, break immunoescaping mechanisms of BCL1 cells...
HPMA-based polymeric conjugate of doxorubicin acting in concert with the host anti-tumour immunity
Šírová, Milada; Rossmann, Pavel; Mrkvan, Tomáš; Strohalm, Jiří; Šubr, Vladimír; Plocová, Daniela; Ulbrich, Karel; Říhová, Blanka
2007 - English
Polymeric conjugate Dox-HPMA-HuIg directly targets the tumour due to the EPR effect, it is not detrimental towards the immune system of the host, and acts in concert with the anti-tumour immune responses Polymerní konjugát Dox-HPMA-HuIg působí přímo proti nádoru prostřednictvím EPR efektu, nepoškozuje imunitní systém nositele a působí ve shodě s jeho imunitní odpovědí
Keywords:
doxorubicin
Available at various institutes of the ASCR
HPMA-based polymeric conjugate of doxorubicin acting in concert with the host anti-tumour immunity
Polymeric conjugate Dox-HPMA-HuIg directly targets the tumour due to the EPR effect, it is not detrimental towards the immune system of the host, and acts in concert with the anti-tumour immune ...
Degradation of humic acids by letter-decomposing basidiomycetes
Šnajdr, Jaroslav; Trögl, Josef; Steffen, K.; Heeg, K.; Hofrichter, M.; Baldrian, Petr
2007 - English
Two strains of saprotrophic basidiomycetes - Hypholoma fasciculare and Rhodocollybia butyracea – were isolated from the oak wood in Xaverov (Prague, Czech Republic). We followed the production of extracellular enzymes in a laboratory setup as well as the composition of microbial community during the colonization of reconstructed soil profile Dva kmeny saprotrofních basidiomycet - Hypholoma fasciculare a Rhodocollybia butyracea - byly izolovány z dubového lesa v Xaverově (Praha). V laboratorním experimentu jsme sledovali produkci extracelulárních enzymů a složení mikrobiálního společenstva v průběhu kolonizace rekonstruovaného půdního profilu
Keywords:
saprotrophic basidiomycetes
Available at various institutes of the ASCR
Degradation of humic acids by letter-decomposing basidiomycetes
Two strains of saprotrophic basidiomycetes - Hypholoma fasciculare and Rhodocollybia butyracea – were isolated from the oak wood in Xaverov (Prague, Czech Republic). We followed the production of ...
Prostorová a časová dynamika distribuce enzymových aktivit v lesních půdách ve vztahu k půdním houbám
Baldrian, Petr; Šnajdr, Jaroslav; Valášková, Vendula; Cajthaml, Tomáš; Merhautová, Věra
2007 - Czech
Aktivita lakázy, Mn-peroxidázy, endo-1,4-β-glukanázy, endo-1,4-β-xylanázy, cellobiohydrolázy, β-glukosidázy a β-xylosidázy v lesní půdě (s dominantním Quercus sp.) byla v průběhu roku silně, ale nikoli konsistentně variabilní. Aktivita enzymů klesala s hloubkou půdy, podobně jako biomasa hub a poměr biomasy hub a bakterií Activity of laccase, Mn-peroxidase, endo-1,4-β-glucanase, endo-1,4-β-xylanase, cellobiohydrolase, β-glucosidase and β-xylosidase in forest soil (with dominant Quercus sp.) exhibited high but inconsistent variation. Enzyme activity decreased with soil depth as well as fungal biomass and the fungal/bacterial biomass ratio
Keywords:
laccase
Available at various institutes of the ASCR
Prostorová a časová dynamika distribuce enzymových aktivit v lesních půdách ve vztahu k půdním houbám
Aktivita lakázy, Mn-peroxidázy, endo-1,4-β-glukanázy, endo-1,4-β-xylanázy, cellobiohydrolázy, β-glukosidázy a β-xylosidázy v lesní půdě (s dominantním Quercus sp.) byla v průběhu roku silně, ale ...
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