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Indirect quantification of doxorubicin nuclear accumulation
Hovorka, Ondřej; Eckschlager, T.; Strohalm, Jiří; Ulbrich, Karel; Říhová, Blanka
2007 - English
This method increased the detection sensitivity more than ten times and allowed us to quantify doxorubicin cleaved from the conjugates and intercalated into nuclear DNA Jedním z hlavních mechanismů účinku anthracyklinového antibiotika doxorubicinu je jaderná akumulace a interkalace do jaderné DNA. Kvantifikace jeho akumulace v jádře nepřímou metodou pomocí snížení fluorescence Hoechst 33342 je výrazně citlivější a přesnější technikou než přímá detekce, založená na fluorescenci doxorubicinu
Keywords:
doxorubicin
Available at various institutes of the ASCR
Indirect quantification of doxorubicin nuclear accumulation
This method increased the detection sensitivity more than ten times and allowed us to quantify doxorubicin cleaved from the conjugates and intercalated into nuclear DNA...
Continuous assays for studying DNA translocation by EcoR124I Type I RM enzyme
Šišáková, Eva; Seidel, R.; Szczelkun, M.; Weiserová, Marie
2007 - English
Using combination of bulk solution and single-molecule assays we have investigated the influence of several mutations in the PD-(D/E)-X-K catalytic motif of HsdR on the DNA translocation and ATPase properties of EcoR124I, the restriction-modification enzyme Type I. The gained results have given us the insight into the possible interdomain interactions between helicase and nuclease domains of the HsdR subunit and the effect of their interactions on DNA translocation Kombinací hromadných experimentů (stopped-flow) s experimenty s jednotlivou molekulou enzymu (magnetická pinzeta) byl studován vliv několik mutaci v katalytickém motivu PD-(D/E)-X-K podjednotky HsdR na DNA translokační a ATPasovou aktivitu restrikčně-modifikačního enzymu EcoR124I. Získané výsledky ukazují na možné interakce helikásové a nukleasové domény podjednotky HsdR a na efekt těchto interakcí na translokaci DNA
Keywords:
dna
Available at various institutes of the ASCR
Continuous assays for studying DNA translocation by EcoR124I Type I RM enzyme
Using combination of bulk solution and single-molecule assays we have investigated the influence of several mutations in the PD-(D/E)-X-K catalytic motif of HsdR on the DNA translocation and ATPase ...
Anti DR5 bound to HPMA: new approach for immunochemotherapy of cancer
Říhová, Blanka; Hovorka, Ondřej; Kopečková, P.; Kopeček, J.
2007 - English
Dimerized antibodies anti-DR5 bound to the synthetic polymer based on HPMA, effectively induce apoptosis of the tumor cells that express TRAIL on their surface. At the same time, the tumor cells are also targeted by cytotoxic drug +doxorubicin bound to the HPMA polymeric conjugate Dimerizované protilátky anti-DR5 vázané na syntetický polymer na bázi HPMA, indukují apoptózu nádorových buněk, které exprimují na svém povrchu TRAIL. Přitom zasahují tytéž nádorové buňky také prostřednictvím cytostatika doxorubicinu, který je rovněž navázán na HPMA
Keywords:
polymeric conjugates
Available at various institutes of the ASCR
Anti DR5 bound to HPMA: new approach for immunochemotherapy of cancer
Dimerized antibodies anti-DR5 bound to the synthetic polymer based on HPMA, effectively induce apoptosis of the tumor cells that express TRAIL on their surface. At the same time, the tumor cells are ...
Establishment of a cancer cell line stably expressing green fluorescent protein
Větvička, David; Josková, Radka; Baumgartnerová, E.; Hovorka, Ondřej; Ulbrich, Karel; Říhová, Blanka
2007 - English
Murine T-cell lymphoma cell line EL-4 was transfected via the nucleoporation and stable transfectants have been established Myší T-lymfomová buněčná linie EL-4 byla nukleoporována a byla ustanoveny stabilní transfektanti
Keywords:
polymeric conjugates
Available at various institutes of the ASCR
Establishment of a cancer cell line stably expressing green fluorescent protein
Murine T-cell lymphoma cell line EL-4 was transfected via the nucleoporation and stable transfectants have been established...
ESTIMATION OF BIOAVAILABILITY OF HYDROPHOBIC ORGANIC POLLUTANTS IN SOIL
Cajthaml, Tomáš; Erbanová, Pavla; Šašek, Václav
2007 - English
The work deals with posibility to estimate bioavailability of organic pollutants in soil mainly with supercritical fluid extraction Práce se zabývá možností odhadu biodostupnosti organických polutantů v půdě zejména pomocí nadkritické fluidní extrakce
Keywords:
organic pollutants
Available at various institutes of the ASCR
ESTIMATION OF BIOAVAILABILITY OF HYDROPHOBIC ORGANIC POLLUTANTS IN SOIL
The work deals with posibility to estimate bioavailability of organic pollutants in soil mainly with supercritical fluid extraction...
The effects of N-(hydroxypropyl)metha-crylamide copolymer containing doxorubicin on mitogen-activated protein kinases signalling pathways
Kovář, Lubomír; Strohalm, Jiří; Chytil, Petr; Plocová, Daniela; Mrkvan, Tomáš; Ulbrich, Karel; Říhová, Blanka
2006 - English
We demonstrated that the mitogen-activated protein kinases (MAPKs) play a crucial role in cancer cell death and survival, however their role seems to be indirect. We confirmed the previously published results showing that different intracellular processes are underway in cancer cells treated with Dox.HCl compared to those incubated with HPMA conjugates Prokázali jsme, že mitogenem aktivované signalizační dráhy (MAPKs) hrají důležitou roli při smrti a proliferaci buněk, ačkoli je jejich role nepřímá. Potvrdili jsme předchozí výsledky, které ukazují, intracelulární procesy buněk inkubovaných s volným doxorubicinem se liší od procesů buněk inkubovaných s HPMA konjugáty
Keywords:
mitogen; protein kinases
Available at various institutes of the ASCR
The effects of N-(hydroxypropyl)metha-crylamide copolymer containing doxorubicin on mitogen-activated protein kinases signalling pathways
We demonstrated that the mitogen-activated protein kinases (MAPKs) play a crucial role in cancer cell death and survival, however their role seems to be indirect. We confirmed the previously published ...
Modulation of gene expression by promoter mutagenesis in Corynebacterium glutamicum
Holátko, Jiří; Elišáková, Veronika; Prouza, Marek; Phensaijai, M.; Nešvera, Jan; Pátek, Miroslav
2006 - English
Studies of structure and function of promoters in C. glutamicum were used in graded expression of genes involved in biosynthetic pathway of valine (ilvBNC, ilvD, ilvE) and in competing or branching-off pathways (ilvA, leuA). Combination of particular promoter mutants resulted in tuning the whole pathway to overall higher flux of the intermediates through the pathway and in high production of valine Byla studována struktura a funkce promotorů v C. glutamicum a výsledky využity k regulaci exprese genů biosyntézy valinu (ilvBNC, ilvD, ilvE) a genů konkurenčních nebo odvětvujících se biosyntetických drah (ilvA, leuA). Kombinace mutací v určitých promotorech vedly k modulaci celé biosyntetické dráhy s následným zvýšením toku meziproduktů a zvýšenou produkcí valinu
Keywords:
c. glutamicum; promoters
Available at various institutes of the ASCR
Modulation of gene expression by promoter mutagenesis in Corynebacterium glutamicum
Studies of structure and function of promoters in C. glutamicum were used in graded expression of genes involved in biosynthetic pathway of valine (ilvBNC, ilvD, ilvE) and in competing or ...
Searching for Corynebacterium glutamicum promoters regulated differently in various growth phases
Phensaijai, M.; Knoppová, Monika; Pátek, Miroslav; Nešvera, Jan
2006 - English
Promoters of the amino acid-producing bacterium Corynebacterium glutamicum regulated differently in various phases of growth have been searched for. It was found that activity of promoter of the sigE gene coding for alternative sigma factor of RNA polymerase was strongly increased when the culture entered the stationary phase of growth Byly vyhledávány promotory producenta aminokyselin Corynebacterium glutamicum různě regulované v různých fázích růstu. Bylo zjištěno, že aktivita promotoru genu sigE, kódujícího alternativní sigma-faktor RNA polymerasy, byla silně zvýšena ve stacionární fázi růstu
Keywords:
corynebacterium glutamicum; promoters
Available at various institutes of the ASCR
Searching for Corynebacterium glutamicum promoters regulated differently in various growth phases
Promoters of the amino acid-producing bacterium Corynebacterium glutamicum regulated differently in various phases of growth have been searched for. It was found that activity of promoter of the sigE ...
ESI-MS/MS Study of Native and Modified Haemoglobin Complexes
Hesso, A.; Tornaeus, J.; Havlíček, Vladimír
2006 - English
Tandem mass spectroemtry is a suitable method to analyse haemoglobin complexes and resolve their structure Tandemová hmotnostní spektrometrie je vhodnou metodou k analýze hemoglobinových komplexů a zjišťování jejich truktury
Keywords:
haemoglobin; mass spectrometry
Available at various institutes of the ASCR
ESI-MS/MS Study of Native and Modified Haemoglobin Complexes
Tandem mass spectroemtry is a suitable method to analyse haemoglobin complexes and resolve their structure...
Is There an HLA-B27 Associated Peptide Responsible for Ankylosin Spondylitis
Novák, Petr; Man, Petr; Stodůlková, Eva; Ivašková, E.; Flieger, Miroslav
2006 - English
Peptides eluted from HLA-B27 molecules of peripheral blood leukocytes (PBL) of healthy blood donors (HLT) and AS patients were analyzed by MALDI mass spectrometry and ESI mass spectrometry. Similarity within HLT and AS data sets was evaluated Peptidy eluované z HLA-B27 PBL molecul zdravých a AS dárců byly analyzovány metodou MALDI a ESI. Výsledná data zdravých a AS dárců byla vyhodnocována
Keywords:
biomarkers; fourier transform; mass spectrometry
Available at various institutes of the ASCR
Is There an HLA-B27 Associated Peptide Responsible for Ankylosin Spondylitis
Peptides eluted from HLA-B27 molecules of peripheral blood leukocytes (PBL) of healthy blood donors (HLT) and AS patients were analyzed by MALDI mass spectrometry and ESI mass spectrometry. Similarity ...
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