No (286)
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Application of pressurized hot water for etching of microfluidics
Vašina, M.; Grym, Jakub; Foret, František
2013 - English
The subject of this study is a new method of glass etching and its application in microfluidics. Commonly, hydrofluoric acid is used for glass etching. In this study we have tested a new method of glass etching using pressurized hot water. Test motives were prepared using photolitography and metal sputtering on the glass substrate and the etching was performed in a newly developed device capable to compress and heat water to its critical point. Electron microscopy and profilometry have been used for analyzing the surface of the etched glass substrate.
Keywords:
microfluidics; pressurized hot water
Available at various institutes of the ASCR
Application of pressurized hot water for etching of microfluidics
The subject of this study is a new method of glass etching and its application in microfluidics. Commonly, hydrofluoric acid is used for glass etching. In this study we have tested a new method of ...
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
Benkovská, Dagmar; Flodrová, Dana; Bobálová, Janette; Laštovičková, Markéta
2013 - English
N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies.
Keywords:
glycosylation; post-translational modification; barley; N-glycopetptides
Available on request at various institutes of the ASCR
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the ...
Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Matalová, Eva; Adamová, Eva; Klepárník, Karel
2013 - English
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as signalling centres in embryonic development, stem cells or tumour cells. To localize cells of interest within an organ/tissue, histological sections are widely used. However, the methods of choice for further analysis of such samples are mostly limited to histochemistry, immunohistochemistry and in situ hybridization.
Keywords:
apoptosis; laser capture microdissection; cell analysis
Available on request at various institutes of the ASCR
Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as ...
Proteomic evaluation of MCF-7 human breast cancer cells after treatment with retinoic acid isomers: Preliminary insights
Flodrová, Dana; Benkovská, Dagmar; Macejová, D.; Bialešová, L.; Brtko, J.; Bobálová, Janette
2012 - English
The effects of 9-cis retinoic acid and all-trans retinoic acid in human MCF-7 breast cancer line have been investigated. The total cell proteins were extracted and separated on 1D SDS-PAGE. The proteins were subsequently in-gel digested by trypsin and identified by MALDI-TOF/TOF.
Keywords:
retinoids; breast cancer; proteomic analysis
Available at various institutes of the ASCR
Proteomic evaluation of MCF-7 human breast cancer cells after treatment with retinoic acid isomers: Preliminary insights
The effects of 9-cis retinoic acid and all-trans retinoic acid in human MCF-7 breast cancer line have been investigated. The total cell proteins were extracted and separated on 1D SDS-PAGE. The ...
Depoziční tok ozonu nad smrkovým porostem a jeho korelace s čistou ekosystémovou produkcí
Zapletal, M.; Cudlín, Pavel; Chroust, P.; Urban, Otmar; Pokorný, Radek; Edwards-Jonášová, Magda; Czerný, Radek; Janouš, Dalibor; Taufarová, Klára; Medlen, A.; Večeřa, Zbyněk; Mikuška, Pavel; Skácel, F.
2011 - Czech
Srovnání výsledků měření a modelování depozičního a stomatálního toku ozonu u smrku ztepilého v lokalitě Bílý Kříž v červenci a srpnu 2008. Zvýšené koncentrace ozonu snižují čistou ekosystémovou produkci, zvláště při vyšších teplotách a vysoké intenzitě globálního záření. Measured and modelled results of ozone deposition and stomatal flux to a Norway spruce forest in locality Bílý Kříž in July and August 2008 were compared. Enhanced concentration of ozone decreased net ecosystem production, especially at higher temperatures and high intensity of global radiation.
Keywords:
ozone deposition flux; ozone stomatal flux; net ecosystem production
Available at various institutes of the ASCR
Depoziční tok ozonu nad smrkovým porostem a jeho korelace s čistou ekosystémovou produkcí
Srovnání výsledků měření a modelování depozičního a stomatálního toku ozonu u smrku ztepilého v lokalitě Bílý Kříž v červenci a srpnu 2008. Zvýšené koncentrace ozonu snižují čistou ekosystémovou ...
Koncentrace anhydridů monosacharidů v PM1 a PM2,5 aerosolu
Mikuška, Pavel; Křůmal, Kamil; Vojtěšek, Martin; Večeřa, Zbyněk
2011 - Czech
Analýza anhydridů monosacharidů (levoglukosan, mannosan, galaktosan) ve dvou velikostních frakcích aerosolu (PM1 a PM2.5) a porovnání koncentrací v průběhu zimní a letní kampaně v Brně a Šlapanicích v roce 2009, identifikace hlavních emisních zdrojů. Analysis of monosacharide anhydrides (levoglucosan, mannosan, galactosan) in two aerosol size fractions (PM1 and PM2.5) and comparison of concentrations during winter and summer campaigns in Brno and Šlapanice in 2009, identification of main emission sources.
Keywords:
monosacharide anhydrides; organic tracer; biomass burning
Available at various institutes of the ASCR
Koncentrace anhydridů monosacharidů v PM1 a PM2,5 aerosolu
Analýza anhydridů monosacharidů (levoglukosan, mannosan, galaktosan) ve dvou velikostních frakcích aerosolu (PM1 a PM2.5) a porovnání koncentrací v průběhu zimní a letní kampaně v Brně a Šlapanicích v ...
Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk; Mikuška, Pavel; Dočekal, Bohumil; Vojta, Šimon; Míšek, Ivan; Moravec, Pavel; Smolík, Jiří
2011 - English
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
Keywords:
inhalation chamber; chronic exposure studies of small animals; long-term inhalation procedures
Available at various institutes of the ASCR
Unique exposure system for the whole body inhalation experiments with small animals
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of ...
Identifikace zdrojů resuspendovaných pevných částic statistickými metodami
Huzlík, J.; Ličbinský, R.; Mikuška, Pavel
2011 - Czech
Aerosoly ve velikostní frakci PM2.5 a PM10 a pouliční prach byly paralelně vzorkovány v Ostravě a Brně během zimy a léta 2009 a 2010. Zdroje aerosolů, včetně resuspendovaného prachu a jeho příspěvku k celkové koncentraci aerosolů, byly identifikovány pomocí metod receptorového modelování. Parallel collection of aerosol particles in size fractions PM2.5 and PM10 and street dust at the same location was performed in Ostrava and Brno during winter and summer 2009 and 2010. Identification of PM sources including resuspended PM and their contribution to overall PM concentration was done by using receptor modelling methods.
Keywords:
resuspension; aerosol source identification; receptor modelling
Available at various institutes of the ASCR
Identifikace zdrojů resuspendovaných pevných částic statistickými metodami
Aerosoly ve velikostní frakci PM2.5 a PM10 a pouliční prach byly paralelně vzorkovány v Ostravě a Brně během zimy a léta 2009 a 2010. Zdroje aerosolů, včetně resuspendovaného prachu a jeho příspěvku k ...
Rozlišení kmenů rodu Candida pomocí kapilární HPLC
Vykydalová, Marie; Moravcová, Dana; Horká, Marie; Růžička, F.; Kahle, Vladislav
2011 - Czech
Cílem této práce bylo rozlišení kvasinkových kmenů porovnáním jejich chromatografických profilů lyzátů. The aim of this study was to compare chromatographic profiles of lysate of Candida parapsilosis positive and Candida parapsilosis negative using capilary high performance liquid chromatography.
Keywords:
Candida; HPLC; biofilm
Available at various institutes of the ASCR
Rozlišení kmenů rodu Candida pomocí kapilární HPLC
Cílem této práce bylo rozlišení kvasinkových kmenů porovnáním jejich chromatografických profilů lyzátů....
Elektroforetická separace oligosacharidů s vodivostní detekcí
Partyka, Jan; Foret, František
2011 - Czech
Zlepšení separačních vlastností oligosacharidů pomocí derivatizace kvartérní amoniovou značkou. Improvement of separation properties of oligosaccharides by quaternary ammonium labeling.
Keywords:
oligosaccharide; AETMA; capillary electrophoresis
Available at various institutes of the ASCR
Elektroforetická separace oligosacharidů s vodivostní detekcí
Zlepšení separačních vlastností oligosacharidů pomocí derivatizace kvartérní amoniovou značkou.
Improvement of separation properties of oligosaccharides by quaternary ammonium labeling.
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