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A novel cultivation system, composed of glass beads immersed in a liquid medium, used for proteomic studies of Streptomyces population during their life cycle
Weiser, Jaroslav; Nguyen, Liem Duy; Holub, Martin; Nezbedová, Šárka; Virolle, M. - J.; Kalachová, Ladislava; Benada, Oldřich; Kofroňová, Olga; Bezoušková, Silvia
2006 - English
To get a better understanding of the interplay between primary and secondary metabolism and morphological differentiation in Streptomycetes we studied their proteomes in cultivation system resembling their natural environment Studovali jsme proteomy streptomycet v kultivačním systému napodobujícím jejich přírodní životní prostředí s cílem porozumět interakcím mezi primárním a sekundárním metabolizmem a morfologickou diferenciací v průběhu jejich životního cyklu
Keywords:
streptomyces; cultivation system
Available at various institutes of the ASCR
A novel cultivation system, composed of glass beads immersed in a liquid medium, used for proteomic studies of Streptomyces population during their life cycle
To get a better understanding of the interplay between primary and secondary metabolism and morphological differentiation in Streptomycetes we studied their proteomes in cultivation system resembling ...
Multivalent glycomimetics in experimental anticancer immunotherapy
Vannucci, Luca; Pospíšil, Miloslav; Krist, Pavel; Křen, Vladimír; Huliková, Katarína; Luptovcová, Martina; Svoboda, Jan; Kuldová, Markéta; Bezouška, Karel; Rossmann, Pavel; Difato, Francesco; Mosca, F.; Fišerová, Anna
2006 - English
Lectin-like receptors can modulate the cytotoxic, regulatory and dendritic cell responses by their interactions. GN8 multivalent glycoconjugates are able to internalize inside and activate NKR-P1 positive cells inducing production of IFN-gamma and expression of CD69. The final effect is a modulation of anticancer immunity with reduction of cancer growth Interakce lektinových receptorů ovlivňuje imunitní odpověď cytotoxických, regulačních a dendritických buněk. Glykokonjugáty spouštějí tyto pochody aktivací NKR-P1 pozitivních buněk a následným zvýšením produkce IFNg a expresí CD69 antigenu
Keywords:
immunotherapy; lectin-like receptors
Available at various institutes of the ASCR
Multivalent glycomimetics in experimental anticancer immunotherapy
Lectin-like receptors can modulate the cytotoxic, regulatory and dendritic cell responses by their interactions. GN8 multivalent glycoconjugates are able to internalize inside and activate NKR-P1 ...
Anti-tumour efficacy of the conjugate based on N-(2-hydroxypropyl)methacrylamide containing doxorubicin and normal human
Šírová, Milada; Strohalm, Jiří; Rossmann, Pavel; Mrkvan, Tomáš; Šubr, Vladimír; Plocová, Daniela; Ulbrich, Karel; Říhová, Blanka
2006 - English
Polymeric conjugate based on HPMA containing cytotoxic drug (doxorubicin) and human “non-specific” immunoglobulin has a significant anti-tumour effect and does not impair immune functions of the host. C57BL/6 mice with EL-4 lymphoma, when completely cured with the conjugate, were protected against the same tumour due to a specific anti-tumour immune reaction Polymerní konjugát na bázi HPMA s doxorubicinem a normálním lidským imunoglobulinem má významný protinádorový účinek. U myší C57BL/6 může úplně vyléčit syngenní lymfom EL-4. Léčba pravděpodobné přispívá ke stimulaci imunokompetentních buněk, takže vyléčení jedinci jsou po léčbě chráněni proti nádoru specifickou imunitní reakcí
Keywords:
polymeric conjugate; immunology; hpma
Available at various institutes of the ASCR
Anti-tumour efficacy of the conjugate based on N-(2-hydroxypropyl)methacrylamide containing doxorubicin and normal human
Polymeric conjugate based on HPMA containing cytotoxic drug (doxorubicin) and human “non-specific” immunoglobulin has a significant anti-tumour effect and does not impair immune functions of the host. ...
Unmasking tumour for the immune systém: HPMA-based copolymers with pH-controlled release of doxorubicin in anti-cancer therapy
Mrkvan, Tomáš; Šírová, Milada; Etrych, Tomáš; Chytil, Petr; Strohalm, Jiří; Plocová, Daniela; Ulbrich, Karel; Říhová, Blanka
2006 - English
Therapy with HPMA-based conjugates triggers effective anti-tumour immunity early during the course of the treatment. Activated immune system, in addition to direct cytotoxic effect the drug, is responsible for the eradication of established tumour Terapie konjugáty na bázi HMPA vyvolává účinnou a časnou protinádorovou odpověď. Aktivovaný imunitní systém se spolu s přímým cytotoxickým účinkem léčiva podílí na odhojení ustaveného nádoru
Keywords:
hpma; immunology; doxorubicin
Available at various institutes of the ASCR
Unmasking tumour for the immune systém: HPMA-based copolymers with pH-controlled release of doxorubicin in anti-cancer therapy
Therapy with HPMA-based conjugates triggers effective anti-tumour immunity early during the course of the treatment. Activated immune system, in addition to direct cytotoxic effect the drug, is ...
Pseudallescheria boydii secreted cyclic peptide - the potential marker of fungal infection
Sklenář, Jan; Žabka, Martin; Šulc, Miroslav; Olšovská, Jana; Havlíček, Vladimír
2006 - English
The work consisted in isolation of fungal secondary metabolite, its purification by means of liquid chromatography, and structure determination by mass spectrometry and nulear magnetic resonance. Due to the stability of the cycliv peptide the molekule will be tested as marker of fungal infection in anímal model Práce se týká izolace a purifikace fugálního sekundárního metabolitu pomocí kapalinové chromatografie a určení jeho struktury pomocí hmotové spektrometrie a nukleární magnetické rezonance. Díky jeho vysoké stabilitě bude zmíněný cyklický peptid testován zda by nemohl sloužit jako biomarker fugální infekce
Keywords:
cyclic peptide; pseudalleschria boydii
Available at various institutes of the ASCR
Pseudallescheria boydii secreted cyclic peptide - the potential marker of fungal infection
The work consisted in isolation of fungal secondary metabolite, its purification by means of liquid chromatography, and structure determination by mass spectrometry and nulear magnetic resonance. Due ...
HPLC and UPLC determination of lincomycin traces in fermentation broth of genetically modified Streptomaces sp. with solid phase extraction on HLB OASIS
Olšovská, Jana; Jelínková, Markéta; Man, Petr; Flieger, Miroslav; Koběrská, Markéta
2006 - English
UPLC and HPLC method development for lincomycin determination in fermentation broth (sample matrix) in concentration range 0.1-100 µg/ml. Method application for lincomycin determination after different types of genetic manipulations in lincomycin biosynthetic cluster Poster referuje o vývoji nové UPLC a HPLC metody stanovení linkomycinu ve fermentační tekutině v koncentračním rozmezí 0.1-100 µg/ml. Metoda je aplikována na stanovení linkomycinu pro různé typy geneticky manipulovaných kmenů v linkomycinovém biosyntetickém klastru
Keywords:
hplc; uplc
Available at various institutes of the ASCR
HPLC and UPLC determination of lincomycin traces in fermentation broth of genetically modified Streptomaces sp. with solid phase extraction on HLB OASIS
UPLC and HPLC method development for lincomycin determination in fermentation broth (sample matrix) in concentration range 0.1-100 µg/ml. Method application for lincomycin determination after ...
Changes of physiology of Saccharomyces cerevisiae strains with PDR transporter genes deletions
Hlaváček, Otakar; Váchová, Libuše; Palková, Z.
2006 - English
We focused on comparison of the development and behaviour of S. cerevisiae strains overproducing various PDR transporters to those that have genes for some of them deleted. Besides a spectrum of physiological parameters, we analysed also expression changes of selected genes and measured reduced cytochrome spectra of these strains to find out the state of their respiration Zaměřili jsem se na vývoj a chování kmenů S. cerevisiae s nadprodukcí různých PDR transportérů a kmenů s jejich delecemi. Kromě řady fyziologických parametrů byly analyzovány změny v expresi vybraných genů a měřena spektra cytochromů pro zjištění stavu respirace
Keywords:
saccharomyces cerevisiae; pdr transporters
Available at various institutes of the ASCR
Changes of physiology of Saccharomyces cerevisiae strains with PDR transporter genes deletions
We focused on comparison of the development and behaviour of S. cerevisiae strains overproducing various PDR transporters to those that have genes for some of them deleted. Besides a spectrum of ...
Immunologic aspects of colorectal carcinoma progression in conventional and germ-free animal models
Vannucci, Luca; Štěpánková, Renata; Kozáková, Hana; Tlaskalová, Helena; Fišerová, Anna; Pospíšil, Miloslav
2006 - English
Our data support the findings that mild chronic inflammation (“physiologic inflammation”) sustained by commensal flora in the bowel of conventional animals may be the cause of an effective anticancer response by both mucosal and general immunity Naše výsledky podporují zjištění, že mírný chronický zánět (“fyziologický zánět”) navozuje lepší protinádorovou imunitní odpověď ve srovnání s bezmikrobními jedinci
Keywords:
colorectal carcinoma; germ-free animal models; chronic inflammation
Available at various institutes of the ASCR
Immunologic aspects of colorectal carcinoma progression in conventional and germ-free animal models
Our data support the findings that mild chronic inflammation (“physiologic inflammation”) sustained by commensal flora in the bowel of conventional animals may be the cause of an effective anticancer ...
The plastoskeleton protein FtsZ and chloroplast fission in green algae
Vítová, Milada; Hendrychová, Jana; Bišová, Kateřina
2006 - English
FtsZ protein, an ancestral homologue of eukaryotic tubulin, is the key effector in regulation of bacterial division. In plants, the FtsZ protein participates in chloroplast division. We have studied the chloroplast division in the green unicellular alga Scenedesmus quadricauda. To uncouple chloroplast and nucleocytoplasmic division cycles, we used 5-fluorodeooxyuridine (FdUrd). The amount and localization of FtsZ during the cell cycle was followed using immunoblotting and immunofluorescence staining on semi-thin sections. Antibody against FtsZ reacts with 49kD protein in Scenedesmus quadricauda and this protein is localized in chloroplast.The level of FtsZ protein increases during growth phase with maximum in time of chloroplast division. Localization of FtsZ protein is changing during the cell cycle, but we have not observed Z-ring, described in higher plants FtsZ protein, ancestrální homolog eukaryotického tubulinu, je klíčovým proteinem bakteriálního dělení. U rostlin se FtsZ účastní dělení chloroplastů. Studovali jsme dělení chloroplastů u zelené jednobuněčné řasy Scenedesmus quadricauda. Pro odpřažení chloroplastového a nukleocytoplazmatického cyklu dělení jsme použili inhibitor 5-fluorodeooxyuridin (FdUrd). Množství a lokalizaci FtsZ během buněčného cyklujsme sledovali pomocí imunoblotingu a imunofluorescence na polotenkých řezech. Protilátka proti FtsZ reaguje s 49kD proteinem u Scenedesmus quadricauda a tento protein je lokalizován v chloroplastu. Hladina FtsZ proteinu stoupá během růstové fáze s maximem v době dělení chloroplastů. Lokalizace FtsZ proteinu se během buněčného cyklu mění, ale nepozorovali jsme Z-ring popsaný u vyšších rostlin
Keywords:
ftsz protein
Available at various institutes of the ASCR
The plastoskeleton protein FtsZ and chloroplast fission in green algae
FtsZ protein, an ancestral homologue of eukaryotic tubulin, is the key effector in regulation of bacterial division. In plants, the FtsZ protein participates in chloroplast division. We have studied ...
Novel proteomic approach to screen human colostrum and milk for cytokines
Kverka, Miloslav; Burianová, Jaroslava; Lodinová-Žádniková, R.; Kocourková, I.; Tučková, Ludmila; Tlaskalová, Helena
2006 - English
The aim of this study was to determine the cytokine profile of human milk and colostrum using novel proteomic approach microarray. The cytokine profile of human milk and colostrum shows considerable interindividual variability Článek se zabývá použitím protilátkového mikročipu při určování cytokinového spektra mateřského mléka a kolostra
Keywords:
human colostrum; milk; newborn
Available at various institutes of the ASCR
Novel proteomic approach to screen human colostrum and milk for cytokines
The aim of this study was to determine the cytokine profile of human milk and colostrum using novel proteomic approach microarray. The cytokine profile of human milk and colostrum shows considerable ...
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