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Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora; Šalplachta, Jiří; Roth, Michal
2016 - English
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of bioactive proteins in Sambucus nigra L. branches.
Keywords:
extraction of bioactive proteins; MALDI-TOF/TOF mass spectrometry; Sambucus nigra L. branches; SDS-PAGE; PHWE
Available in digital repository of the ASCR
Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of ...
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Večeřová, Kristýna; Mikuška, Pavel; Oravec, Michal; Kozáčiková, Michaela; Pompeiano, Antonio; Coufalík, Pavel; Urban, Otmar
2016 - English
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week exposure to airborne CdONPs of ecologically\nrelevant size and concentration significantly reduced photosynthetic light use efficiency\nin plants as well as contents of photosynthetic pigments and saccharides. In contrary,\namino acid content was increased under CdONPs. These changes were further\nmodulated by previous growth temperature.
Keywords:
Picea abies; terrestrial ecosystems; CdO nanoparticles
Available in digital repository of the ASCR
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week ...
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
Malá, Zdeňka; Gebauer, Petr; Boček, Petr
2016 - English
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic focusing on an inverse electromigration dispersion\n(EMD) gradient. The technique is based on a robust electrolyte system providing a\ngradient of required properties, suitable for routine analyses of trace amounts of weak\nacids with pKa values between approx. 6.5 and 9. Examples analyses of several\nchlorophenols and sulfonamides are presented, with LODs around 3x10-9 M for spiked\ndrinking water without any sample pretreatment.
Keywords:
capillary electrophoresis; electrospray-ionization mass spectrometric detection; chlorophenols; sulfonamides
Available in digital repository of the ASCR
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic ...
A novel nanospray liquid junction interface for versatile CE-MS
Křenková, Jana; Haselberg, R.; Somsen, G. W.; Foret, František
2016 - English
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a hybrid microfabricated plastic liquid junction sprayer allowing to perform analysis in standard (30-100 micro m ID) separation capillaries. The interface incorporates a self-aligning port for coupling of the separation capillary as well as ports for automated flushing of both the liquid junction and the separation capillary. The interface permits performing CE both with and without electroosmotic flow. The use of large bore capillaries allows injection and preconcentration of larger sample volumes, favoring detection sensitivity. Different compositions of the spray liquid and BGE can be used when needed.
Keywords:
capillary electrophoresis; mass spectrometry; liquid junction interface
Available in digital repository of the ASCR
A novel nanospray liquid junction interface for versatile CE-MS
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a ...
Screening of acyclovir permeation through skin using alaptide
Bobálová, Janette; Černíková, A.; Bobáľ, P.; Jampílek, J.
2016 - English
This investigation deals with the affection of the permeation of acyclovir through fullthickness\npig ear skin using a Franz diffusion cell from the donor vehicle of propylene\nglycol/water (1:1) using synthesised (S)-8-methyl-6,9-diazaspiro[4.5]decane-7,10-\ndione, alaptide as a potential transdermal permeation enhancer.
Keywords:
acyclovir permeation; chemical permeation enhancers; skin permeation experiments
Available on request at various institutes of the ASCR
Screening of acyclovir permeation through skin using alaptide
This investigation deals with the affection of the permeation of acyclovir through fullthickness\npig ear skin using a Franz diffusion cell from the donor vehicle of propylene\nglycol/water (1:1) ...
Determination of levoglucosan in urban aerosol
Coufalík, Pavel; Mikuška, Pavel; Čmelík, Richard
2016 - English
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by LC-MS.
Keywords:
monosaccharide anhydrides; urban aerosol; levoglucosan
Available in digital repository of the ASCR
Determination of levoglucosan in urban aerosol
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by ...
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Brtko, J.; Toporová, L.; Macejová, D.; Bialešová, L.; Flodrová, Dana; Bobálová, Janette
2016 - English
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Keywords:
retinoic acid; retinoid receptors; protein pattern
Available in digital repository of the ASCR
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch; Klepárník, Karel
2016 - English
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
Keywords:
single-cell analysis; apoptotic cells; non-apoptotic cells
Available in digital repository of the ASCR
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during ...
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
Lačná, J.; Foret, František; Kubáň, Petr
2016 - English
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new CE-LIF system. Then the derivatization process\nof MDA by TBA was optimized. The ability to use this method for the detection of\nMDA in a biological samples, such as EBC and plasma, was also demonstrated. For the\nfirst time it was possible to detect MDA in EBC samples by CE. Other biomarkers are\nnow being studied.
Keywords:
CE-LIF; malondialdehyde; biomarkers; lipids
Available in digital repository of the ASCR
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new ...
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Kubáň, Pavel; Boček, Petr; Seip, K. F.; Gjelstad, A.; Pedersen-Bjergaard, S.
2016 - English
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel approach requires μL volumes of samples and organic\nsolvents, which form consecutive immiscible plugs in a transparent polymeric capillary\nand the extraction process is accelerated by application of d.c. voltage. Number of the\nplugs, their composition, volume and sequence in the extraction capillary can be chosen\nspecifically for each particular application and high variability and selectivity of the\nextraction process can be achieved. In this contribution, we demonstrate suitability of\na five-phase μ-EME system for simultaneous extractions of cations and anions from\nurine and for selective extractions of basic analytes based on their acid-base strength\nfrom plasma.
Keywords:
micro-electromembrane extraction; sample pretreatment techniques; biological samples
Available in digital repository of the ASCR
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel ...
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