Construction and application of the amperometric uric acid biosensors based on the covalent immobilization of uricase by different strategies
Tvorynska, Sofiia; Barek, J.; Josypčuk, Bohdan
2021 - English
In this work, a promising combination of a biosensor based on the\nenzymatic mini-reactor with the detection principle of four-electron\nreduction of the consumed oxygen at highly negative potential has\nbeen developed for uric acid determination using flow injection\nanalysis. The construction of the biosensor provides a spatial\nsegregation of the biorecognition (uricase-based mini-reactor) and\ndetection (tubular detector of silver solid amalgam (TD-p-AgSA))\nparts. To find out the most appropriate enzyme immobilization\nprotocol, three different strategies of the covalent attachment for\nuricase from Bacillus fastidiosus have been compared. It was found\nthat the biosensor with the mini-reactor based on the covalent\nattachment of uricase via glutaraldehyde to -NH2 functionalized\nmesoporous silica powder MCM-41 showed extremely high stability\n(>1 year) and reusability (at least 600 measurements) The biosensor's\npractical applicability was confirmed by successful determination\nof uric acid in human urine.
Keywords:
amalgam electrode; amperometric biosensor; uric acid
Available in digital repository of the ASCR
Construction and application of the amperometric uric acid biosensors based on the covalent immobilization of uricase by different strategies
In this work, a promising combination of a biosensor based on the\nenzymatic mini-reactor with the detection principle of four-electron\nreduction of the consumed oxygen at highly negative potential ...
IMPOSING BIAXIAL STRAIN ON 2D LAYERED MATERIALS BY LIQUID-INDUCED SWELLING OF SUPPORTING POLYMER
Sampathkumar, Krishna; Pekárek, J.; Frank, Otakar
2021 - English
2D layered materials promise to revolutionize the field of electronics, photonics, optoelectronics, energy storage, and sensing, etc. 2D materials have exceptional mechanical properties, with critical elongation >10%. Employing the strain to manipulate the electronic structure of these 2D materials could lead to further improvement of their implementation in many aspects. The ease of manipulation of their electronic structure can be one of the critical factors for their utilization in photonic devices. Apart from the strain, which decreases (increases) the bandgap energy at the rate of similar to 100 meV under 1% of biaxial tension (compression), also the layer number causes bandgap energy change of, e.g., 0.5 eV between bulk (1.3 eV) and monolayer MoS2 (1.8 eV). In our work, we focus on using the swelling behavior of PMMA/SU8 polymer in methanol to impose the strain on 2D layered materials. In the first trials, we have shown that it is possible to reach a strain gradient from 0 to similar to 0.5% of biaxial strain via simple swelling of polymer substrates, both for graphene [1] and transition metal di-chalcogenides (TMDC) like MoS2. Raman spectroscopy was used to probe the lattice strain in the materials through measuring changes of vibrational frequencies, and photoluminescence was used to probe the strain-induced bandgap character and energy in TMDC at room temperature. The surface corrugation of the 2D material after the soaking was recorded with the help of atomic force microscope (AFM).
Keywords:
raman-spectroscopy; graphene; monolayer; bubbles; 2D material; graphene; tmdc; strain engineering; biaxial strain
Available in digital repository of the ASCR
IMPOSING BIAXIAL STRAIN ON 2D LAYERED MATERIALS BY LIQUID-INDUCED SWELLING OF SUPPORTING POLYMER
2D layered materials promise to revolutionize the field of electronics, photonics, optoelectronics, energy storage, and sensing, etc. 2D materials have exceptional mechanical properties, with critical ...
ELECTROCHEMICAL STUDY OF CuSCN INORGANIC HOLE-TRANSPORT MATERIAL FOR SOLAR CELLS PREPARED BY ELECTRODEPOSITION FROM AQUEOUS SOLUTION
Vlčková Živcová, Zuzana; Mansfeldová, Věra; Bouša, Milan; Kavan, Ladislav
2021 - English
A comparative study is reported for electrodeposited copper(I) thiocyanate layers (ca. 500 nm) on two types of conductive/semiconductive substrates: i) carbon (boron-doped diamond_BDD, glass-like carbon_GC), and ii) carbon-free F-doped SnO2 conducting glass (FTO). SEM and Raman evidence that electrodeposition from aqueous solution results in homogenous CuSCN layers with dominant thiocyanate ion bounded to copper through its S-end (Cu−SCN bonding), as in spin-coated CuSCN layers. Electrochemical impedance spectroscopy (EIS) confirms the p-type semiconductivity of layers with a flatband potential from 0.1 to 0.18 V vs. Ag/AgCl depending on the substrate type, and the acceptor concentration (NA) of 5 x 1020cm-3 in all cases. The flatband potentials determined from Mott-Schottky plots (EIS) are in good agreement with the Kelvin probe measurements. The blocking quality of CuSCN layers was tested using Ru(NH3)63+/2+ redox probe. CuSCN deposited on BDD substrate exhibits better blocking properties compared to CuSCN deposited on FTO.
Keywords:
electrodeposition; CuSCN; hole transport material
Available in digital repository of the ASCR
ELECTROCHEMICAL STUDY OF CuSCN INORGANIC HOLE-TRANSPORT MATERIAL FOR SOLAR CELLS PREPARED BY ELECTRODEPOSITION FROM AQUEOUS SOLUTION
A comparative study is reported for electrodeposited copper(I) thiocyanate layers (ca. 500 nm) on two types of conductive/semiconductive substrates: i) carbon (boron-doped diamond_BDD, glass-like ...
A comparative study of covalent glucose oxidase and laccase immobilization techniques at powdered supports for biosensors fabrication
Tvorynska, Sofiia; Barek, J.; Josypčuk, Bohdan; Nesměrák, K.
2020 - English
In order to develop the optimal strategy and to deepen the knowledge in the field of enzyme immobilization, three different techniques of covalent binding for two enzymes (glucose oxidase and laccase) at powdered surfaces were compared. Immobilization protocol was optimized by changing supports (two mesoporous silica powders (SBA−15, MCM−41) and a cellulose powder), the functionalized\ngroups introduced at support surfaces (−NH and −COOH), and the methods of activation (glutaraldehyde and carbodiimide). Amino and carboxyl functionalized mesoporous silica and cellulose powders\nwere prepared by silanization using (3-aminopropyl)triethoxysilane and carboxyethylsilanetriol, respectively. It was found that coupling of both enzymes by their –NH groups through glutaraldehyde to -NH functionalized supports, in particular SBA15−NH and cellulose−NH for glucose oxidase, MCM41−NH for laccase, showed the highest activity and the best stability.
Keywords:
biosensors; covalent immobilization; enzymatic reactor
Fulltext is available at external website.
A comparative study of covalent glucose oxidase and laccase immobilization techniques at powdered supports for biosensors fabrication
In order to develop the optimal strategy and to deepen the knowledge in the field of enzyme immobilization, three different techniques of covalent binding for two enzymes (glucose oxidase and laccase) ...
Photocatalytic concrete screeds with self-cleaning and antimicrobial function
Bíbová, Hana; Šubrt, Jan; Pližingrová, Eva; Jakubičková, M.; Sázavská, T.; Dohnálek, P.; Hykrdová, Lenka; Jirkovský, Jaromír
2020 - English
Photocatalytic concrete screeds with self-cleaning and antimicrobial function have been developed and tested. These building materials contain sand grains with deposited composite TiO2/SiO2 layers. Two types of the screeds, grey and white, were prepared and their photoactivity and material characteristics were compared. Commercial TiO2 photocatalysts from several manufacturers were employed. Effects of different parameters, such as temperature of the coated sand calcination, humidity, and surface abrasion, were followed. Mechanical properties of the screeds were analyzed by SEM/XRD microscopy. Photocatalytic activity was tested by means of NOx oxidation (ISO norm 22197-1/2007). Antibacterial activity was evaluated by modified ISO norm 27447/2009 with Bacillus subtilis as a model organism.
Keywords:
Antimicrobial activity; NOx; Photodegradation; Self-cleaning screed; sem
Available in digital repository of the ASCR
Photocatalytic concrete screeds with self-cleaning and antimicrobial function
Photocatalytic concrete screeds with self-cleaning and antimicrobial function have been developed and tested. These building materials contain sand grains with deposited composite TiO2/SiO2 layers. ...
On UV-Vis Spectroelectrochemistry of Luteolin-7-O-Glucoside
Castano, A.; Sokolová, Romana; Degano, I.
2019 - English
The UV-Vis spectroelectrochemical cell containing an optically transparent platinum net used as the working electrode was tested in two regimes of electrochemical measurement, cyclic voltammetry and chronoamperometry, respectively. A three electrode setup used silver quasi reference electrode and platinum electrode serving as a counter electrode. The calibration of the spectroelectrochemical cell was performed for one-electron oxidation processes as well as for two-electron oxidations. The spectral changes during the oxidation of luteolin-7-O-Glucoside gave the information about the electroactive chromophores in molecule. Resulted spectra were compared with luteolin. The identification of products of oxidation was done by complementary analytical techniques HPLC-DAD and HPLC-ESI-MS/MS.
Keywords:
spectroelectrochemistry; oxidation; luteolin; antioxidants
Available in digital repository of the ASCR
On UV-Vis Spectroelectrochemistry of Luteolin-7-O-Glucoside
The UV-Vis spectroelectrochemical cell containing an optically transparent platinum net used as the working electrode was tested in two regimes of electrochemical measurement, cyclic voltammetry and ...
Continuous monitoring of anthraquinone-based anticancer drugs by amperometric technique
Skalová, Štěpánka; Fischer, J.; Barek, J.; Navrátil, Tomáš; Krejčí, J.; Kučerová, R.; Vyskočil, V.
2019 - English
This contribution is focused on the development of electroanalytical methods for the monitoring of anthraquinone-based anticancer drugs in physiological solution by combination of liquid-flow system and dialysis catheter, possibly inserted into blood circulation of patients. For this purpose, amperometric detection with dual glassy carbon electrode was developed and derivate of these drugs, anthraquinone-2-sulphonate, was used as a model compound. Two different flow rates of carrier solution (physiological solution) were tested (specifically, 1 and 5 mu L min(-1)) and the dependence of peak currents of anthraquinone-2-sulphonate on its concentration was verified
Keywords:
amperometric detection; anthracyclines; anthraquinone-2-sulphonate
Fulltext is available at external website.
Continuous monitoring of anthraquinone-based anticancer drugs by amperometric technique
This contribution is focused on the development of electroanalytical methods for the monitoring of anthraquinone-based anticancer drugs in physiological solution by combination of liquid-flow system ...
Long-Term Sustainability of Research Infrastructures
Hrušák, Jan; Harrison, A.; Lenoir, L.
2017 - English
The second volume of the ESFRI Scripta series is dedicated to\nthe outcomes of the ad hoc Working (WG) Group on Long-Term\nSustainability (LTS) of Research Infrastructures.\nThe LTS WG was created by ESFRI in 2016 to comprehensively respond\nto the Conclusions of the Competitiveness Council of the European\nUnion of 27th May 2016 that „underlines the importance of ensuring\nLong-Term Sustainability of Research Infrastructures and invites the\nCommission to prepare together with ESFRI and relevant stakeholders a\ntargeted action plan“.\nESFRI has previously developed its concept of lifecycle of the Research\nInfrastructures in the framework of the Roadmap evaluation exercise,\nand at all stages of the lifecycle different aspects of sustainability were\nidentified. The LTS WG has analysed the Long-Term Sustainability\nof Research Infrastructures from a broad perspective taking the\nESFRI viewpoint well beyond the mere economical analysis. Scientific\nexcellence is the condition sine qua non for sustainability throughout\nthe entire RI lifecycle, and its persistence is crucial in the long-term\noperational phase. Excellence in science and outstanding quality services\nto the users are imperative for the successful performance of each\nResearch Infrastructure, and they are addressed in the monitoring and\nreviewing processes by ESFRI.
Keywords:
sustainability; research infrastructures; ESFRI
Available in digital repository of the ASCR
Long-Term Sustainability of Research Infrastructures
The second volume of the ESFRI Scripta series is dedicated to\nthe outcomes of the ad hoc Working (WG) Group on Long-Term\nSustainability (LTS) of Research Infrastructures.\nThe LTS WG was created by ...
High resolution infrared spectroscopy as diagnostic tool for combustion and plasma chemistry
Zelinger, Zdeněk; Nevrlý, Václav; Grigorová, Eva; Bitala, P.; Dostál, Michal; Suchánek, Jan; Kubát, Pavel; Engst, Pavel; Ferus, Martin; Kubelík, Petr; Civiš, Svatopluk
2017 - English
Monitoring of transient species within combustion experiments (laminar flames, shock-tubes, flow reactors, etc.) is still relatively challenging task especially if application of non-invasive, i.e. optical detection methods is required. High resolution infrared spectroscopy is based on observation of the fine rotation structure that accompanies vibration transitions and thus provides direct information essential to characterization of both molecular structure and reaction dynamics. Thanks to its outstanding advantage enabling unambiguous assignment of specific molecular system according to its spectral feature, it can serve as a helpful tool for exploring complex reaction mechanisms as well as chemical reactivity of individual species present in laboratory flames or plasmas.\nPrevious studies gaining new insights into combustion and plasma chemistry as well as our recent advances targeted towards application of high resolution infrared spectroscopy for species concentration measurement in laminar flames are summarized here below.
Keywords:
spectroscopy; plasma chemistry; infrared spectra
Available in digital repository of the ASCR
High resolution infrared spectroscopy as diagnostic tool for combustion and plasma chemistry
Monitoring of transient species within combustion experiments (laminar flames, shock-tubes, flow reactors, etc.) is still relatively challenging task especially if application of non-invasive, i.e. ...
Wavelength modulation spectroscopy for multicomponent ana-lytics of biomass burning tracers
Dostál, Michal; Válek, V.; Suchánek, Jan; Kristlová, E.; Roupcová, P.; Zelinger, Zdeněk; Nevrlý, Václav; Bitala, P.; Vašinek, M.; Kubát, Pavel; Ferus, Martin; Civiš, Svatopluk
2017 - English
The potential of Tunable Diode Laser Absorption Spectroscopy (TDLAS) for monitoring of several species produced by biomass burning is in focus of this work. The infrared spectra of selected molecules (HCOOH, C2H2, CH3CN, N2O, CH3OH, CH3COCH3) are measured in laboratory conditions and the selectivity and of this method is demonstrated.
Keywords:
spectroscopy; biomass burning tracers; TDLAS system
Available in digital repository of the ASCR
Wavelength modulation spectroscopy for multicomponent ana-lytics of biomass burning tracers
The potential of Tunable Diode Laser Absorption Spectroscopy (TDLAS) for monitoring of several species produced by biomass burning is in focus of this work. The infrared spectra of selected molecules ...
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