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Speciační analýzy s využitím generování hydridů
Matoušek, Tomáš
2023 - Czech
Přednáška prezentovala různé způsoby využití postupu generování hydridů- selektivní generování, postkolonové generování a generování s kryoseparací- v prvkových speciačních analýzách. Příklady z naší laboratoře zahrnovaly aplikace z oblasti klinické analýzy, analýzy potravin a přírodních vod. The lecture presented employing of different ways of hydride generation- selective generation, postcolumn generation and cryotrapping- useful of element speciation analysis. Examples from our laboratory covered applications from the field of clinical, food and natural water analysis.
Keywords:
hydrides generation; inductively coupled plasma mass spectrometry; speciation analysis
Available on request at various institutes of the ASCR
Speciační analýzy s využitím generování hydridů
Přednáška prezentovala různé způsoby využití postupu generování hydridů- selektivní generování, postkolonové generování a generování s kryoseparací- v prvkových speciačních analýzách. Příklady z naší ...
Generování těkavých sloučenin – účinná technika vnášení vzorku pro ICP-MS
Musil, Stanislav
2023 - Czech
V rámci této přednášky byly prezentovány různé techniky generování těkavých sloučenin (specií), možnosti spojení s ICP-MS a nedávné výsledky naší laboratoře v oblasti fotochemického generování těkavých specií přechodných prvků. Various techniques of volatile species generation, the ways of coupling to ICP-MS and recent results of our laboratory in the field of photochemical vapor generation of transition metals were presented in this lecture.
Keywords:
volatile species generation; inductively coupled plasma mass spectrometry; photochemical vapor generation
Available at various institutes of the ASCR
Generování těkavých sloučenin – účinná technika vnášení vzorku pro ICP-MS
V rámci této přednášky byly prezentovány různé techniky generování těkavých sloučenin (specií), možnosti spojení s ICP-MS a nedávné výsledky naší laboratoře v oblasti fotochemického generování ...
Přenosný kapalinový chromatograf jako nový nástroj stanovení nebezpečných látek
Šesták, Jozef; Lunerová, K.; Vaněk, J.
2021 - Czech
Přenosný kapalinový chromatograf představuje nový přístroj kompaktních rozměrů, který umožňuje realizaci HPLC analýz v podmínkách mimo laboratoř. Byl využit pro vývoj certifikované metodiky pro detekci a stanovení vybraných mykotoxinů v komplexních vzorcích. The portable liquid chromatograph is a new instrument of compact dimensions, which enables the implementation of HPLC analyzes in conditions outside the laboratory. It was used for the development of a certified methodology for the detection and determination of selected mycotoxins in complex samples.\n\n
Keywords:
portable liquid chromatograph; mykotoxins; miniaturization
Available on request at various institutes of the ASCR
Přenosný kapalinový chromatograf jako nový nástroj stanovení nebezpečných látek
Přenosný kapalinový chromatograf představuje nový přístroj kompaktních rozměrů, který umožňuje realizaci HPLC analýz v podmínkách mimo laboratoř. Byl využit pro vývoj certifikované metodiky pro ...
Epitachophoretic preconcentration of proteins
Hrušková, Helena; Voráčová, Ivona; Foret, František
2021 - English
In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide low analysis time, narrow shape of the protein zone, and eliminate overheating at the center of the device. In the next step, the developed method was tested on the preconcentration of proteins: cytochrome c, myoglobin, and hemoglobin. After several adjustments myoglobin and hemoglobin were also uccessfully preconcentrated. After evaluation of pilot results, this technique provides up to fiftyfold preconcentration and recovery of over 70 % for each protein. The next plan is to preconcentrate proteins from complex biological matrices such as urine or blood plasma.
Keywords:
epitachophoresis; isotachophoresis; preparative concentration
Available on request at various institutes of the ASCR
Epitachophoretic preconcentration of proteins
In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide ...
Quantum dot luminescent probe for Caspase 3/7 imaging inside cells
Procházková, Markéta; Klepárník, Karel
2021 - English
Two step synthesis of luminescent probe based on ligand-exchange in the first step and the specific reaction of amino group in the second step was optimized. The luminescence properties of the final product were checked by time course of\nactive recombinant caspase protein reaction under the model conditions in fluorimeter. The novel luminescent probe enables long-time imaging of active caspases in living cells or tissues.
Keywords:
caspase; fluorescence; quantum dot
Available on request at various institutes of the ASCR
Quantum dot luminescent probe for Caspase 3/7 imaging inside cells
Two step synthesis of luminescent probe based on ligand-exchange in the first step and the specific reaction of amino group in the second step was optimized. The luminescence properties of the final ...
Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria
Farka, Z.; Mickert, M. J.; Hlaváček, Antonín; Poláchová, V.; Kostiv, Uliana; Pastucha, M.; Horák, Daniel; Gorris, H. H.; Skládal, P.
2020 - English
The recent progress in the field of immunoassays has been driven by introduction of various kinds of nanomaterials. In particular, photon-upconversion nanoparticles (UCNPs) proved to be excellent immunoassay labels due to their ability to emit light of shorter wavelengths under near-infrared excitation (anti-Stokes emission), which prevents autofluorescence, minimizes light scattering, and thus reduces the optical background interference. These unique photoluminescent properties allow counting of individual biomolecules labeled with UCNPs by conventional wide-field epiluminescence microscopy and enable the development of single-molecule (digital) immunoassays. We have introduced a novel label based on UCNPs conjugated with streptavidin via poly(ethylene glycol) and applied it in a digital upconversion-linked immunosorbent assay (ULISA) for the detection of a cancer biomarker prostate specific antigen (PSA). The digital readout based on counting of individual immunocomplexes improved the sensitivity 16× compared to conventional analog readout and allowed to reach a limit of detection (LOD) of 23 fg·mL−1 (800 aM). Human serum samples were successfully analyzed achieving an excellent correlation with electrochemiluminescence reference method. The conjugates of UCNPs with streptavidin are also suitable for the detection of pathogenic bacterium Melissococcus plutonius, the causative agent of honeybee disease European foulbrood. The ULISA assay provided an LOD of 340 CFU·mL−1 and successfully analyzed real samples of bees, larvae and bottom hive debris. Due to the high reliability and relatively simple detection scheme, the digital ULISA can pave the way for a new generation of digital immunoassays with a strong potential for commercialization.
Keywords:
single-molecule detection; photon-upconversion nanoparticle; bioconjugation
Available at various institutes of the ASCR
Photon-upconversion nanoparticles for single-molecule immunosensing of cancer biomarkers and bacteria
The recent progress in the field of immunoassays has been driven by introduction of various kinds of nanomaterials. In particular, photon-upconversion nanoparticles (UCNPs) proved to be excellent ...
Chemical vapor generation of cadmium for analytical atomic spectrometry
Sagapova, Linda; Kodríková, Barbora; Svoboda, Milan; Musil, Stanislav; Kratzer, Jan
2020 - English
Chemical vapor generation of cadmium volatile compounds was optimized in order to determine trace Cd concentrations by atomic absorption spectrometry (AAS). Several reaction modifiers based on inorganic salts and complexes of CrIII+, CoII+, TiIII+, TiIV+ were tested to improve analytical performance and generation efficiency.
Keywords:
chemical vapor generation; cadmium; atomic absorption spectrometry
Available on request at various institutes of the ASCR
Chemical vapor generation of cadmium for analytical atomic spectrometry
Chemical vapor generation of cadmium volatile compounds was optimized in order to determine trace Cd concentrations by atomic absorption spectrometry (AAS). Several reaction modifiers based on ...
Surface modification of nanoparticles for sustaining sensitivity of surface-enhanced raman spectrometric measurements in salinated environment
Týčová, Anna; Přikryl, Jan; Vaňhara, P.; Klepárník, Karel; Foret, František
2020 - English
Surface-enhanced Raman spectrometry (SERS) represents a powerful method for analysis of a broad spectrum of analytes ranging from inorganic ions to biomolecules of high complexity. It combines the potential\nof Raman spectrometry for a definite identification of an analyte with remarkable sensitivity achieved by the surface enhancement effect occurring on metal nanoparticles. While low ionic strength influences positively\nthe sensitivity of the SERS measurement, a higher level of inorganic salts leads to fast ruining of colloidal character, which completely devastates the effect of the surface enhancement. The common stabilization of\nnanoparticles by a layer of polymers has a negative impact on the SERS sensitivity since it shields the nanoparticle surface from the analytes. In this work, we aim at the development of the bi-ligand system of\nnanoparticles surface modification for improved stability of colloid in saline solution at sustaining the potential for sensitive SERS analyses. The proposed system relies on the binding of 3-mercaptopropionic acid and\nthiolated polyethylene glycol in a suitable ratio onto the nanoparticle surfaces. While the short chains of the acid sustain the accessibility of the surface for analytes, the polymeric structures act as a steric barrier\npreventing colloid aggregation.
Keywords:
bi-ligand; nanoparticle; saline solution; surface-enhanced Raman spectrometry
Available at various institutes of the ASCR
Surface modification of nanoparticles for sustaining sensitivity of surface-enhanced raman spectrometric measurements in salinated environment
Surface-enhanced Raman spectrometry (SERS) represents a powerful method for analysis of a broad spectrum of analytes ranging from inorganic ions to biomolecules of high complexity. It combines the ...
Effect of metal sensitizers on photochemical vapor generation of bismuth for analytical atomic spectrometry
Vyhnanovský, Jaromír; Yildiz, D.; Musil, Stanislav
2019 - English
Three new sensitizers with a significant positive effect on the photochemical vapor generation of bismuth were described. Cobalt was found to be the most effective and led to a significant enhancement of the signal.
Keywords:
photochemical volatile species generation; bismuth; AAS
Available at various institutes of the ASCR
Effect of metal sensitizers on photochemical vapor generation of bismuth for analytical atomic spectrometry
Three new sensitizers with a significant positive effect on the photochemical vapor generation of bismuth were described. Cobalt was found to be the most effective and led to a significant enhancement ...
Generování hydridů ve spojení s hmotnostní spektrometrií s indukčně vázaným plazmatem
Matoušek, Tomáš
2019 - Czech
Přehledný text popisující využití techniky generování těkavých hydridů v hmotnostní spektrometrií s indukčně vázaným plazmatem včetně speciačních analýz. An overview describing technique of volatile hydride generation in connection with inductively coupled plasma mass spectrometry including speciation analysis.
Keywords:
hydride generation; ICP-MS; speciation analysis
Available at various institutes of the ASCR
Generování hydridů ve spojení s hmotnostní spektrometrií s indukčně vázaným plazmatem
Přehledný text popisující využití techniky generování těkavých hydridů v hmotnostní spektrometrií s indukčně vázaným plazmatem včetně speciačních analýz....
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