24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Franěk, František; Kohout, Ladislav; Eckschlager, T.
2003 - English
Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma, i.e. a hybrid ymphocyte cell line, was cultured either in standard serum-free medium, or in diluted medium in which the cell grew inder nutritional stress. Steady-state parameters of semicon-finuous cultures conducted at 24-epibrassinolide concentra-ions from 10~16 M to 1CT9 M were evaluated. Typical effects of he agent that were observed both in standard and in diluted i [media were suppression of intracellular antibody level, increa- / ,ed value of mitochondrial membrane potential, higher frac- / ion of cells in the G(/Gl phase and lower fraction of cells in / he S phase. Alleviation of nutritional stress was observed in j (cultures conducted in diluted media. Viable cell density was j ignificantly higher at 24-epibrassinolide concentrations 10~13[ knd 10~12 M. Results of this exploratory study show that thej plant hormone 24-epibrassinolide may induce alterations of he cell division mechanism of the energetic metabolism, and of secreted protein synthesis in a mammalian cell line.
Keywords:
Brassinolides; cell division
Available at various institutes of the ASCR
24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured ...
Microbial Degradation of TCA in Norway Spruce
Forczek, Sándor; Matucha, Miroslav; Gryndler, Milan; Albrechtová, Jolana
2003 - English
Keywords:
biodegradation; chloroacetic acid; phyllosphere
Available at various institutes of the ASCR
Microbial Degradation of TCA in Norway Spruce
Construction of chromosome-specific BAC libraries in plants
Janda, Jaroslav; Šafář, Jan; Bartoš, Jan; Doleželová, Marie; Kovářová, Pavlína; Valárik, Miroslav; Čihalíková, Jarmila; Šimková, Hana; Doležel, Jaroslav
2003 - English
Keywords:
flow sorted chromosomes; BAC librares; wheat
Available at various institutes of the ASCR
Construction of chromosome-specific BAC libraries in plants
Analysis of plant genomes using flow cytometry
Bartoš, Jan; Doleželová, Marie; Šafář, Jan; Janda, Jaroslav; Kovářová, Pavlína; Čihalíková, Jarmila; Valárik, Miroslav; Šimková, Hana; Doležel, Jaroslav
2003 - English
Keywords:
flow cytometry; flow sorted chromosomes; FISH
Available at various institutes of the ASCR
Analysis of plant genomes using flow cytometry
Approaches to elucidating the role of subcellular compartmentation in the regulation of cytokinin metabolism
Kiran, N.; Genkov, T.; Malbeck, Jiří; Brzobohatý, Břetislav
2002 - English
Keywords:
cytokinins; subcellular compartmentation; Nicotiana tabacum
Available at various institutes of the ASCR
Approaches to elucidating the role of subcellular compartmentation in the regulation of cytokinin metabolism
Biotic Stress and Genetic Transformation Promote Activity of Antioxidant Enzymes in PSSU-IPT Tobacco
Synková, Helena; Semorádová, Šárka; Čeřovská, Noemi
2002 - English
Biotic Stress and Genetic Transformation Promote Activity of Antioxidant Enzymes in PSSU-IPT Tobacco.
Keywords:
Biotic Stress; PSSU IPT Tobacco
Available at various institutes of the ASCR
Biotic Stress and Genetic Transformation Promote Activity of Antioxidant Enzymes in PSSU-IPT Tobacco
Biotic Stress and Genetic Transformation Promote Activity of Antioxidant Enzymes in PSSU-IPT Tobacco.
Genetic Variability Between Different Isolates of Potato Mop-top Virus (PMTV)
Rosecká, Pavla; Moravec, Tomáš; Filigarová, Marie; Dědič, P.; Čeřovská, Noemi
2002 - English
We determined partial nucleotide sequences of several different field isolates of PMTV with well-characterized biological properties from the Czech Republic and Denmark. Subsequently we were trying to elucidate the correlation between nucleotide sequences of various isolates, their biological properties and geographical origin. From the comparison with other known sequences we can conclude, that PMTV genome is relatively conserved.
Keywords:
Potato mop-top virus; recombinant coat protein; Escherichia coli
Available at various institutes of the ASCR
Genetic Variability Between Different Isolates of Potato Mop-top Virus (PMTV)
We determined partial nucleotide sequences of several different field isolates of PMTV with well-characterized biological properties from the Czech Republic and Denmark. Subsequently we were trying to ...
Construction of BAC DNA libraries and their characterization using DNA markers
Šafář, Jan
2002 - English
Keywords:
BAC libraries; genomics,
Available at various institutes of the ASCR
Construction of BAC DNA libraries and their characterization using DNA markers
STRETCHED CHROMOSOMES AS TARGETS FOR HIGH-RESOLUTION PHYSICAL MAPPING IN PLANTS USING FISH
Valárik, Miroslav; Vrána, Jan; Rychtářová, Kateřina; Doleželová, Marie; de Jong, H.; Doležel, Jaroslav
2001 - English
Keywords:
STRETCHED CHROMOSOMES; PHYSICAL MAPPING IN PLANTS
Available at various institutes of the ASCR
STRETCHED CHROMOSOMES AS TARGETS FOR HIGH-RESOLUTION PHYSICAL MAPPING IN PLANTS USING FISH
Cytoskeleton role in plant defense response to fungal pathogen attack
Sedlářová, Michaela; Binarová, Pavla; Lebeda, A.
2001 - English
Keywords:
Cytoskeleton; fungal pathogen attack
Available at various institutes of the ASCR
Cytoskeleton role in plant defense response to fungal pathogen attack
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