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Structure of an immunoactive polysaccharide isolated from Korean mulberry fruit (Morus alba L.)
Bleha, R.; Lee, J. S.; Capek, P.; Pohl, Radek; Kim, H. B.; Choi, D. J.; Lee, S.; Lee, J.; Jang, S. J.; Synytsya, A.; Park, Y. I.
2014 - English
A water-soluble polysaccharide (JS-MP-1) was isolated from the water extract of Korean white mulberry fruits (Morus alba L.) by ethanol precipitation and then purified by DEAE-cellulose ion exchange chromatography. Obtained final polysaccharide (1600 KDa) consisted of galactose, arabinose and rhamnose as major neutral sugars. JS-MP-1 also contains galacturonic and glucuronic acids (4:1). Sugar linkage, FTIR and correlation NMR analyses confirmed that it is a rhamnogalacturonan type I (RG I), which contains the alternating sequence of 1,4-alpha-D-GalAp and 1,2-alpha-L-Rhap units in the backbone. Neutral sugar side chains of JS-MP1 were identified as (1 -> 5)-alpha-L-arabinan and arabinogalactan type II (AG II) having the (1 -> 6)-beta-D-galactan core. The arabinan side chains are bound to the backbone at the O-4 position of some alpha-L-Rhap units, while the way of linkage between RG I and AG II chains is unclear. It was demonstrated that JS-MP-1 significantly stimulates murine macrophage RAW 264.7 cells to release chemokines (RANTES and MIP-1 alpha) and proinflammatory cytokines like INF-alpha and IL-6, and induce the iNOS and COX-2 gene expression, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that JS-MP-1 can act as a potent immunomodulator and these observations may support the applicability of this polysaccharide or the water extracts of mulberry fruit can be used as an immunotherapeutic adjuvant or health beneficial food material.
Keywords:
pectic polysaccharides
Available at various institutes of the ASCR
Structure of an immunoactive polysaccharide isolated from Korean mulberry fruit (Morus alba L.)
A water-soluble polysaccharide (JS-MP-1) was isolated from the water extract of Korean white mulberry fruits (Morus alba L.) by ethanol precipitation and then purified by DEAE-cellulose ion exchange ...
Development and applications of ionization techniques in ambient mass spectrometry
Rejšek, Jan; Vrkoslav, Vladimír; Cvačka, Josef
2014 - English
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization techniques in the ambient mass spectometry. Herein, they were investigated in two research areas: (1) TLC analysis. (2) Localization of insect defense glands.
Keywords:
ambient mass spectrometry; desorption electrospray ionization; desorption atmospheric pressure photoionization; thin-layer chromatography; insect defense compounds; mass spectrometry imaging
Available at various institutes of the ASCR
Development and applications of ionization techniques in ambient mass spectrometry
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization ...
Analysis and characterization of antimicrobial peptides by capillary electromigration methods
Tůmová, Tereza; Monincová, Lenka; Čeřovský, Václav; Kašička, Václav
2014 - English
High-performance capillary electromigration methods (zone electrophoresis, affinity electrophoresis, isotachophoresis and micellar electrokinetic chromatography) have been applied to analysis and physicochemical characterization of halictines – antimicrobial peptides isolated from the bee Halictus sexcinctus.
Keywords:
antimicrobial peptides; capillary electrophoresis; coating
Available at various institutes of the ASCR
Analysis and characterization of antimicrobial peptides by capillary electromigration methods
High-performance capillary electromigration methods (zone electrophoresis, affinity electrophoresis, isotachophoresis and micellar electrokinetic chromatography) have been applied to analysis and ...
Analytical methods Used in the Studies on Chemical Ecology of Termites
Krasulová, Jana; Hanus, Robert; Sillam-Dusses, David; Žáček, Petr; Jiroš, Pavel; Šobotník, Jan; Kalinová, Blanka; Bosáková, Z.; Valterová, Irena
2012 - English
In this paper, I review our recent results on the trail-following communication in the Neotropical termite Glossotermes oculas (Serritermitidae) and the defensive chemistry of the African termite Psammotermes hybostoma (Rhinotermitidae). These findings belong to our current project focused on chemical ecology of critical termite genera from families Rhinotermitidae and Serritermitidae.
Keywords:
defensive compounds; GC-MS; GCxGC-MS; termites; trail-following pheromone
Available at various institutes of the ASCR
Analytical methods Used in the Studies on Chemical Ecology of Termites
In this paper, I review our recent results on the trail-following communication in the Neotropical termite Glossotermes oculas (Serritermitidae) and the defensive chemistry of the African termite ...
Photoluminiscent diamond nanoparticles - new diagnostic and visualization probes
Cígler, Petr
2012 - English
Selected problems connected with surface modification of nanosized diamond crystals, attachment of chemical sensing architectures to them and their colloidal stability were addressed. Fluorescent nanodiamonds modified by proper functional structures were shown as unique unbleachable nanoprobes for construction of future sensors useful in biological and medicinal applications.
Keywords:
nanodiamond; fluorescence probe; nanosensor
Available at various institutes of the ASCR
Photoluminiscent diamond nanoparticles - new diagnostic and visualization probes
Selected problems connected with surface modification of nanosized diamond crystals, attachment of chemical sensing architectures to them and their colloidal stability were addressed. Fluorescent ...
Influence of the O-phosphorylation of serine, threonine and tyrosine in proteins on the amidic N-15 chemical shielding anisotropy tensors
Emmer, J.; Vavrinská, A.; Sychrovský, Vladimír; Benda, Ladislav; Kříž, Z.; Koča, J.; Boelens, R.; Sklenář, V.; Trantírek, L.
2012 - English
Density functional theory was employed to study the influence of O-phosphorylation of serine, threonine, and tyrosine on the amidic N-15 chemical shielding anisotropy (CSA) tensor in the context of the complex chemical environments of protein structures.
Keywords:
15N CSA; protein phosphorylation; DFT
Available at various institutes of the ASCR
Influence of the O-phosphorylation of serine, threonine and tyrosine in proteins on the amidic N-15 chemical shielding anisotropy tensors
Density functional theory was employed to study the influence of O-phosphorylation of serine, threonine, and tyrosine on the amidic N-15 chemical shielding anisotropy (CSA) tensor in the context of ...
Molecular simulations unravel preference of haloalkanes for the air/water interface
Habartová, Alena; Minofar, Babak; Obisesan, A.; Roeselová, Martina
2012 - English
Halogenated organics are emitted into the atmosphere from a variety of sources of both natural and anthropogenic origin (Finlayson-Pitts et al., 2000). Their uptake at the surface of aerosols can affect their reactivity, for example in processes which take part in ozone destruction due to production of reactive chlorine, bromine and iodine radicals. This study presents results of molecular dynamic (MD) simulations carried out to investigate the interaction of short-chain haloalkanes with aqueous surfaces. We report on a free energy minimum that the haloalkanes exhibit at the air/water interface.
Keywords:
haloalkanes; aqueous solvation; molecular dynamics simulations
Available at various institutes of the ASCR
Molecular simulations unravel preference of haloalkanes for the air/water interface
Halogenated organics are emitted into the atmosphere from a variety of sources of both natural and anthropogenic origin (Finlayson-Pitts et al., 2000). Their uptake at the surface of aerosols can ...
Application of capillary electrophoresis in forensic science: Analysis of fireworks
Eliáš, Milan; Maier, V.; Knob, R.
2012 - English
In all samples of fireworks, K+, Na+, Ca2+ and Mg2+ ions were proved and quantified by the method of capillary zone electrophoresis using water solution of 10 mM 4-aminopyridine, titrated with hydroxyisobutyric acid to the pH 4.3, as background electrolyte and indirect UV-absorption detection at 200 nm. Pb2+ or Ba2+ ions were proved in few samples. The other selected inorganic cations were not proved in any sample of selected fireworks. NH4+ ions were not detected in any pre-blast samples, but they were established in some post-blast (glazed tiles) samples.
Keywords:
capillary zone electrophoresis; metal cations; fireworks
Available at various institutes of the ASCR
Application of capillary electrophoresis in forensic science: Analysis of fireworks
In all samples of fireworks, K+, Na+, Ca2+ and Mg2+ ions were proved and quantified by the method of capillary zone electrophoresis using water solution of 10 mM 4-aminopyridine, titrated with ...
Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested proteins
Mikšík, Ivan; Lacinová, Kateřina; Zmatlíková, Zdeňka; Sedláková, Pavla; Král, V.; Sýkora, D.; Řezanka, P.; Kašička, Václav
2012 - English
Open-tubular capillary electrochromatography (OT-CEC) using bare gold nanoparticles-based stationary phase has been applied to separation of tryptic peptide fragments of native and glycated proteins, bovine serum albumin and human transferrin. OT-CEC with bare gold nanoparticles stationary phase was found to be a suitable technique for separation of complex peptide mixtures originating from enzymatic (tryptic) digestion of native and glycated bovine serum albumin and human transferrin, and for glycation studies of these proteins.
Keywords:
open-tubular capillary electrochromatography; gold nanoparticles; glycation of proteins
Available at various institutes of the ASCR
Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested proteins
Open-tubular capillary electrochromatography (OT-CEC) using bare gold nanoparticles-based stationary phase has been applied to separation of tryptic peptide fragments of native and glycated proteins, ...
Separation and determination of isoelectric points of GnRH peptides by capillary electrophoresis in conventional and carrier ampholytes-based background electrolytes
Šolínová, Veronika; Poitevin, M.; Barth, Tomislav; Peltre, G.; Kašička, Václav
2012 - English
Two mixtures of human and salmon gonadotropin releasing hormone (GnRH) derived peptides were successfully separated by capillary zone electrophoresis (CZE) in six classical buffer-based BGEs and by carrier ampholytes-based capillary electrophoresis (CABCE) in the same number carrier ampholytes-based isoelectric BGEs of the same or close pH values. In addition, from the pH dependence of peptide electrophoretic mobilities, the isolectric points of separated peptides were determined.
Keywords:
capillary zone electrophoresis; carrier ampholytes; GnRH peptides
Available at various institutes of the ASCR
Separation and determination of isoelectric points of GnRH peptides by capillary electrophoresis in conventional and carrier ampholytes-based background electrolytes
Two mixtures of human and salmon gonadotropin releasing hormone (GnRH) derived peptides were successfully separated by capillary zone electrophoresis (CZE) in six classical buffer-based BGEs and by ...
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