Zoologické dny Ostrava 2024: sborník abstraktů z konference 8.-9. února 2024.
Drozd, P.; Ožana, S.; Bryja, Josef
2024 - český Klíčová slova: zoology; conference Dokument je dostupný na externích webových stránkách. Zoologické dny Ostrava 2024: sborník abstraktů z konference 8.-9. února 2024.

Drozd, P.; Ožana, S.; Bryja, Josef
Ústav biologie obratlovců, 2024

Temoporfin-conjugated upconversion nanoparticles for NIR-induced photodynamic therapy of pancreatic cancer
Shapoval, Oleksandr; Větvička, D.; Kabešová, M.; Engstová, Hana; Horák, Daniel
2024 - anglický Photodynamic therapy (PDT), a clinically approved cancer treatment strategy, has the potential to cure pancreatic cancer with minimal side effects. PDT primarily uses visible wavelengths to directly activate hydrophobic photosensitizers, which may be insufficient for deep-seated cancer cells in clinical practice due to poor penetration. Upconversion nanoparticles (UCNPs) serve as an indirect excitation source to activate photosensitizers (PSs) in the NIR region, overcoming the limitations of molecular PSs such as hydrophobicity, non-specificity, and excitation in the UV/Vis region. Here, monodisperse upconversion NaYF4:Yb3+, Er3+, Fe2+ nanoparticles (UCNPs) have been surface-engineered with poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) and temoporfin (mTHPC), a clinically used PDT prodrug, for near-infrared (NIR) light-triggered PDT of pancreatic cancer. The incorporation of Fe2+ ions into the particles increased the fluorescence intensity in the red region matching the activation wavelength of mTHPC. Covalent binding of mTHPC to the surface of UCNP@PMVEMA particles provided colloidally stable conjugates enabling generation of singlet oxygen. In vitro cytotoxicity and photodynamic activity of the particles were evaluated using INS-1E rat insulinoma and Capan-2 and PANC-01 human pancreatic adenocarcinoma cell lines. The PDT efficacy of UCNP@PMVEMA-mTHPC conjugates after irradiation with 980 nm NIR light was tested in vivo in a pilot study on Capan-2 human pancreatic adenocarcinoma growing subcutaneously in athymic nude mice. The intratumoral administration of the nanoconjugates significantly hindered tumor growth and demonstrated promising PDT efficacy against human pancreatic cancer. Klíčová slova: photodynamic therapy; temoporfin; upconversion nanoparticles Plné texty jsou dostupné v digitálním repozitáři Akademie Věd. Temoporfin-conjugated upconversion nanoparticles for NIR-induced photodynamic therapy of pancreatic cancer

Photodynamic therapy (PDT), a clinically approved cancer treatment strategy, has the potential to cure pancreatic cancer with minimal side effects. PDT primarily uses visible wavelengths to directly ...

Shapoval, Oleksandr; Větvička, D.; Kabešová, M.; Engstová, Hana; Horák, Daniel
Ústav makromolekulární chemie, 2024

Zoologické dny Brno 2023: sborník abstraktů z konference 9.-10. února 2023
Bryja, Josef; Horsák, M.; Horsáková, V.
2023 - český Klíčová slova: zoology; conference Dokument je dostupný na externích webových stránkách. Zoologické dny Brno 2023: sborník abstraktů z konference 9.-10. února 2023

Bryja, Josef; Horsák, M.; Horsáková, V.
Ústav biologie obratlovců, 2023

Utilization of electrochemically induced cleavage of various sulfonimides
Salvadori, Karolína
2023 - anglický In this work, we describe the unexpected electrochemical cleavage of sulfonimides, providing a direct pathway to corresponding sulfonamides. For this purpose, 16 substances were synthesized and tested, reflecting the influence of different electron effects of -SO2Ph-R substituents, and the impact of the number of nosyl units present in molecules. In the case of octakis-nosylated calixarenes, the electrochemical cleavage via direct potential-controlled electrolysis was compared with conventional organic synthetic procedures. After electrolysis, all prepared sulfonamides were isolated in excellent yields. Klíčová slova: sulfonimides; green chemistry procedures; electrochemisty Plné texty jsou dostupné v digitálním repozitáři NUŠL Utilization of electrochemically induced cleavage of various sulfonimides

In this work, we describe the unexpected electrochemical cleavage of sulfonimides, providing a direct pathway to corresponding sulfonamides. For this purpose, 16 substances were synthesized and ...

Salvadori, Karolína
Ústav chemických procesů, 2023

Green approach for the preparation of biosourced ionic liquids and the study of their thermodynamic properties\n
Souvenir Zafindrajaona, Mahasoa-Salina
2023 - anglický This presentation will address a green approach to the synthesis of ionic liquids qualified as biosourced (monocatenar or bolaform systems) from specific agro-resources, according to several principles of green chemistry. As these solvents could be used to solubilize lignocellulosic biomass and/or extract bioactive compounds (from plant sourcing), the physicochemical characteristics (density, viscosity and heat capacity) of pure ILs or mixtures of IL/water must be determined. Preliminary results concerning this last point will be presented. Klíčová slova: biosourced ionic liquids; lignocellulosic biomass; thermodynamic properties Plné texty jsou dostupné v digitálním repozitáři NUŠL Green approach for the preparation of biosourced ionic liquids and the study of their thermodynamic properties\n

This presentation will address a green approach to the synthesis of ionic liquids qualified as biosourced (monocatenar or bolaform systems) from specific agro-resources, according to several ...

Souvenir Zafindrajaona, Mahasoa-Salina
Ústav chemických procesů, 2023

Cultivation of Limnospira maxima and utilization of its fresh biomass in human nutrition
Vásquez, Claudia
2023 - anglický This work aims to enhance productivity, efficiency and sustainability of L. maxima cultivation as well as to determine the safe microbiological conditions for the nutritional use of fresh spirulina biomass. Various parameters such as light intensity, light spectral composition, temperature, pH, CO2, and culture media composition can be optimized. A series of experiments were performed to find the most suitable configuration to cultivate L. maxima, defined by cyanobacterium productivity and yield as well as other metabolites production such as phycocyanin (C-PC) and carotenoids. Klíčová slova: Limnospira maxima; fresh biomass; human nutrition Plné texty jsou dostupné v digitálním repozitáři NUŠL Cultivation of Limnospira maxima and utilization of its fresh biomass in human nutrition

This work aims to enhance productivity, efficiency and sustainability of L. maxima cultivation as well as to determine the safe microbiological conditions for the nutritional use of fresh spirulina ...

Vásquez, Claudia
Ústav chemických procesů, 2023

Organic fluorine content and change during thermal treatmen
Hušek, Matěj
2023 - anglický We have previously described how pyrolysis affected the content of selected PFAS representatives.4,5 However, we could not determine with certainty whether they were merely cleaved or mineralised. Therefore, we focused on the change in organic fluorine content (part of PFAS) and analysed its thermal behaviour in inert (sea sand) and heterogeneous (sewage sludge) material. Klíčová slova: organic fluorine; sewage sludge; thermal treatment Plné texty jsou dostupné v digitálním repozitáři NUŠL Organic fluorine content and change during thermal treatmen

We have previously described how pyrolysis affected the content of selected PFAS representatives.4,5 However, we could not determine with certainty whether they were merely cleaved or mineralised. ...

Hušek, Matěj
Ústav chemických procesů, 2023

Separation of exosomes from polydisperse suspension in microfluidic devices
Paříková, Anna
2023 - anglický The work included fine-tuning the experimental methodology, writing an in-house code for exosome tracking based on a one-way\napproach, and performing a parametric study to investigate the separation potential of the microdevice as a function of channel geometry, flow rate, and viscosity. Preliminary results show that viscoelasticmicrofluidics can be used as an alternative to conventional e xosome separation techniques. Klíčová slova: separation techniques; exosomes; microfluidic devices Plné texty jsou dostupné v digitálním repozitáři NUŠL Separation of exosomes from polydisperse suspension in microfluidic devices

The work included fine-tuning the experimental methodology, writing an in-house code for exosome tracking based on a one-way\napproach, and performing a parametric study to investigate the separation ...

Paříková, Anna
Ústav chemických procesů, 2023

HIGHLY-TIME RESOLVED ELEMENTAL SOURCE APPORTIONMENT OF PM2.5 AT AN URBAN TRAFFIC SITE IN PRAGUE
Windell, Laurence Christian; Pokorná, Petra; Ondráček, Jakub; Schwarz, Jaroslav; Lhotka, Radek; Vodička, Petr; Zíková, Naděžda; Hopke, P.K.; Ždímal, Vladimír
2023 - anglický This work aims to quantify metal emissions from traffic at an urban traffic site in Prague, Czech Republic, identify key tracers of emissions, and investigate exhaust and non-exhaust emissions observable in fine particulate matter (PM2.5). This project signifies the first high-time resolution elemental analysis of Prague aerosols. Klíčová slova: source apportionment; traffic; online Plné texty jsou dostupné v digitálním repozitáři NUŠL HIGHLY-TIME RESOLVED ELEMENTAL SOURCE APPORTIONMENT OF PM2.5 AT AN URBAN TRAFFIC SITE IN PRAGUE

This work aims to quantify metal emissions from traffic at an urban traffic site in Prague, Czech Republic, identify key tracers of emissions, and investigate exhaust and non-exhaust emissions ...

Windell, Laurence Christian; Pokorná, Petra; Ondráček, Jakub; Schwarz, Jaroslav; Lhotka, Radek; Vodička, Petr; Zíková, Naděžda; Hopke, P.K.; Ždímal, Vladimír
Ústav chemických procesů, 2023

INFLUENCE OF RESPIRATORY TRACT CONDITIONS ON PARTICLE SIZE DISTRIBUTIONS GENERATED BY COMMERCIALLY AVAILABLE INHALERS
Mašková, Ludmila; Ždímal, Vladimír; Ondráček, Jakub
2023 - anglický Pressurized metered dose inhalers (MDI) and dry powder inhalers (DPI) are an integral part of the treatment of lung diseases such as asthma and chronic obstructive pulmonary disease (COPD) (Vichrow et al., 2008). An important characteristic of particles dosed by MDI and DPI is particle size distribution (PSD) (Byron, 1990). This may vary depending on the formulation, but also on the temperature and relative humidity (RH) changes during the transport from the inhaler to the human respiratory tract. The aim of this study was to determine the influence of RH and inspiratory flow rates on the PSD\ncharacteristics and predict deposition efficiency of inhalable particles in the lungs. Klíčová slova: number size distribution; inhaler; hygroscopicity Plné texty jsou dostupné v digitálním repozitáři NUŠL INFLUENCE OF RESPIRATORY TRACT CONDITIONS ON PARTICLE SIZE DISTRIBUTIONS GENERATED BY COMMERCIALLY AVAILABLE INHALERS

Pressurized metered dose inhalers (MDI) and dry powder inhalers (DPI) are an integral part of the treatment of lung diseases such as asthma and chronic obstructive pulmonary disease (COPD) (Vichrow et ...

Mašková, Ludmila; Ždímal, Vladimír; Ondráček, Jakub
Ústav chemických procesů, 2023