NIVB Meeting 2023
Hostomský, Zdeněk; Vácha, R.; Pichová, Iva; Šímová, Šárka
2023 - English NIVB Meeting 2023 – the second meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora on 2. 10. – 5. 10. 2023 will be again after one year an opportunity to meet the participating teams, discuss excellent science, establish collaborations, and present the progress of the project to the members of the International Scientific Advisory Board. Thanks to funding from the Czech Economic Recovery Plan, a number of important results have already been published in high impact journals. Several networking workshops have been organized and scientific collaborations have been established. The meeting in Kutná Hora is therefore a summary of all that has happened in the project over the past year. The main goal of the NIVB is to facilitate cooperation between 30 participating research teams from 8 Czech research institutions, and this goal is gradually being achieved. The NIVB acts as a common communication platform to discuss the desired cooperation of the NIVB research teams, especially those that have not had much contact so far due to their inter-institutional, interdisciplinary or inter-regional distance.\nWe thank all the participants who contributed to the meeting with 30 oral presentations and 67 posters. The NIVB 2022 meeting had the ambition to launch a new series of annual meetings dedicated to virology and bacteriology, and NIVB 2023 fulfils this ambition and continues to aim to inform the wider scientific community about new developments, trends and issues in these disciplines. Keywords: virology; bacteriology; National Institute of Virology and Bacteriology (NIVB); NIVB meeting Available in digital repository of the ASCR NIVB Meeting 2023

NIVB Meeting 2023 – the second meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora on 2. 10. – 5. 10. 2023 will be again after one year an opportunity to meet the ...

Hostomský, Zdeněk; Vácha, R.; Pichová, Iva; Šímová, Šárka
Ústav organické chemie a biochemie, 2023

NIVB Meeting 2022
Šímová, Šárka
2022 - English The first annual meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora on 30.11.–2.12. is an opportunity for participating teams to meet, discuss excellent science, establish collaborations, and introduce the project to the members of our newly established International Science Advisory Board. The primary objective of NIVB is to mediate the cooperation among the 28 participating research teams from 8 Czech research institutions: Institute of Organic Chemistry and Biochemistry, Institute of Molecular Genetic, and Biological Centre of the Czech Academy of Sciences, Charles University in Prague, Masaryk University in Brno, the University of Chemistry and Technology in Prague, and Palacký University in Olomouc. We are delighted that it was possible to arrange the meeting of all the participating research groups, which reflects the scientific breadth of NIVB. The research interests of the participating groups can be broadly assigned into the programs of (1) Pathogen-host interactions, (2) Immunity against viruses and bacteria, and (3) Treatments for viral and bacterial infections. We thank all participants for contributing 28 presentations and 65 posters to the meeting. This conference is a pioneering endeavor, the first meeting that will bridge the virology and bacteriology communities from the Czech Republic. While the research interests of all the participating groups are focused on virology and bacteriology, the contributing laboratories are very diverse in the research methodologies they employ. Therefore, the meeting has the potential to enable the establishment of new scientific collaborations among the participating teams, which is the main objective of NIVB.\nNIVB Meeting 2022 has the ambition to launch a new series of annual meetings on virology and bacteriology and aims at informing wider scientific community about new development, trends and issues in these scientific fields. Keywords: virology; bacteriology; National Institute of Virology and Bacteriology (NIVB); NIVB meeting Available in digital repository of the ASCR NIVB Meeting 2022

The first annual meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora on 30.11.–2.12. is an opportunity for participating teams to meet, discuss excellent science, ...

Šímová, Šárka
Ústav organické chemie a biochemie, 2022

Prague Meeting on Historical Perspectives of Mass Spectrometry: opening of the Czech Mass Spectrometry Museum
Hoskovec, Michal; Cvačka, Josef
2021 - English Keywords: history; mass spectrometry; instruments Fulltext is available at external website. Prague Meeting on Historical Perspectives of Mass Spectrometry: opening of the Czech Mass Spectrometry Museum

Hoskovec, Michal; Cvačka, Josef
Ústav organické chemie a biochemie, 2021

A new method for double bond characterization in lipids by ultraviolet photodissociation mass spectrometry
Rumlová, Barbora; Strmeň, T.; Cvačka, Josef
2021 - English Lipids are structurally diverse biomolecules with vital functions in biological systems. Some of these functions are closely associated with the specific location of the carbon-carbon double bond in the lipid acyl chains. The chain length and the number of unsaturated carbon-carbon bonds can be determined using conventional MS/MS-based structural elucidation methods employing low or higher energy collision-induced dissociation. However, these dissociation techniques do not provide more subtle structural details, for example, the position of unsaturated bonds. Another type of activation method – ultraviolet photodissociation can be used for this purpose. In this study, a new method for characterization of double bond location in lipids acyl chain was developed using 193 nm ultraviolet photodissociation implemented on Orbitrap Fusion Lumos mass spectrometer. This approach is based on the derivatization of the double bond with bis-(5-iodo-[2]pyridyl)-disulfide, subsequent specific cleavages provide a unique diagnostic pair bearing information about the double bond position. Keywords: double bond; lipids; mass spectrometry; ultraviolet photodissociation Fulltext is available at external website. A new method for double bond characterization in lipids by ultraviolet photodissociation mass spectrometry

Lipids are structurally diverse biomolecules with vital functions in biological systems. Some of these functions are closely associated with the specific location of the carbon-carbon double bond in ...

Rumlová, Barbora; Strmeň, T.; Cvačka, Josef
Ústav organické chemie a biochemie, 2021

Enantioseparation and estimation of racemization barriers of selected helquats and their derivatives by capillary electrophoresis using sulfated cyclodextrins as chiral selectors
Sázelová, Petra; Koval, Dušan; Severa, Lukáš; Reyes Gutierrez, Paul Eduardo; Jirásek, Michael; Teplý, Filip; Kašička, Václav
2018 - English Anionic highly sulfated β- and γ-cyclodextrins (S-CDs) and single-isomer heptakis-(2,3-diacetyl-6-sulfato)-β-CD (14Ac-7S-β-CD) were used for chiral separation of [5], [6] and [7]-ring helquats and helquat styryl dyes by capillary electrophoresis (CE) in the background electrolyte composed of 22 mM NaOH/35 mM H3PO4, pH 2.4. The CE method was applied for monitoring of racemization of eight helquats and helical dyes. Rate constant k of conversion, half-life T1/2 of racemization, and activation Gibbs free energy ΔGǂ of interconversion of one enantiomer into the other were calculated from the dependence of natural logarithm of enantiomeric excess (ee) on time using linear regression analysis. Keywords: helquats; racemization barrier; capillary electrophoresis Fulltext is available at external website. Enantioseparation and estimation of racemization barriers of selected helquats and their derivatives by capillary electrophoresis using sulfated cyclodextrins as chiral selectors

Anionic highly sulfated β- and γ-cyclodextrins (S-CDs) and single-isomer heptakis-(2,3-diacetyl-6-sulfato)-β-CD (14Ac-7S-β-CD) were used for chiral separation of [5], [6] and [7]-ring helquats and ...

Sázelová, Petra; Koval, Dušan; Severa, Lukáš; Reyes Gutierrez, Paul Eduardo; Jirásek, Michael; Teplý, Filip; Kašička, Václav
Ústav organické chemie a biochemie, 2018

Determination of binding constants of human insulin complexes with serotonin, dopamine, arginine, and phenol by pressure assisted partial filling affinity capillary electrophoresis
Šolínová, Veronika; Žáková, Lenka; Jiráček, Jiří; Kašička, Václav
2018 - English A new method, pressure assisted partial filling affinity capillary electrophoresis (PF-ACE), has been developed to study noncovalent interactions of the hexamer of human insulin (HI) with cationic ligands, such as phenolic neurotransmitters serotonin and dopamine, and amino acid arginine, or with anionic ligand phenol, in alkaline aqueous solutions. The apparent binding constants, Kb, of the HI-ligand complexes were determined from the dependence of the effective migration time changes of the above ligands on the variable zone lengths of HI dissolved in the background electrolyte and hydrodynamically introduced into the bare fused silica capillary close to the UV detector. The HI interactions with the above ligands were found to be moderately strong, with Kb values in the range 385-1314 L/mol. Keywords: human insulin; ligand; affinity capillary electrophoresis Fulltext is available at external website. Determination of binding constants of human insulin complexes with serotonin, dopamine, arginine, and phenol by pressure assisted partial filling affinity capillary electrophoresis

A new method, pressure assisted partial filling affinity capillary electrophoresis (PF-ACE), has been developed to study noncovalent interactions of the hexamer of human insulin (HI) with cationic ...

Šolínová, Veronika; Žáková, Lenka; Jiráček, Jiří; Kašička, Václav
Ústav organické chemie a biochemie, 2018

Temperature-programed micro-HPLC analysis of fatty acid methyl esters with APCI-MS detection
Vrkoslav, Vladimír; Rumlová, Barbora; Cvačka, Josef
2018 - English HPLC/APCI-MS analysis at microliters-per-minute flow rates was optimized for separation of fatty acid methyl esters (FAMEs). HPLC C18 column with an internal diameter of 0.3 mm and isocratic elution using 99.9 % acetonitrile and 0.1% formic acid were employed. Standard APCI ion source was suitable for detection of FAMEs at 10 μl/min flow rate with the detection limit of micrograms-per-milliliter. APCI-MS spectra with predominant [M + H]+ molecular adducts were observed. The main advantage of micro-flow measurements is the possibility of using a temperature gradient, which significantly reduces retention times of FAMEs with longer aliphatic chain. The significant reduction of solvent consumption is also an important economic and environmental advantage. The positions of double bonds in FAME chains were established using acetonitrile-related adducts and tandem mass spectrometry. The optimized method was applied for analysis of FAMEs in triacylglycerol fraction of black currant seeds oil. Keywords: liquid chromatography; mass spectrometry; atmospheric pressure chemical ionization Fulltext is available at external website. Temperature-programed micro-HPLC analysis of fatty acid methyl esters with APCI-MS detection

HPLC/APCI-MS analysis at microliters-per-minute flow rates was optimized for separation of fatty acid methyl esters (FAMEs). HPLC C18 column with an internal diameter of 0.3 mm and isocratic elution ...

Vrkoslav, Vladimír; Rumlová, Barbora; Cvačka, Josef
Ústav organické chemie a biochemie, 2018