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Growth and Phosphorus Dynamics During Tolypothrix tenuis Cultivation – a Cyanobacterium of Biotechnological Potential
Mušálková, Petra
2024 - English
Here I investigated the course of biomass growth, phosphorus uptake and changes in intracellular phosphorus content in T. tenuis in a batch cultivation. Cultivation experiment was held in multiple bubble columns during a light regime (alternating light and dark periods 16 and 8h). Thanks to this experiment I was able to obtain a growth curve of T. tenuis. The decrease of phosphorus concentration in the medium was disproportionally faster\nthan biomass growth resulting in significant oscillations of phosphorus content in the biomass, which are typical characteristics for LPU. It was also observed, that after all phosphorus was removed from the medium, the algae growth continues over time, while obviously utilizing intracellular phosphorus reserves. These findings are valuable in course of using this cyanobacterium for efficient bioremediation of eutrophic water based on phosphorus removal by targeted algae cultivation.
Available in a digital repository NRGL
Growth and Phosphorus Dynamics During Tolypothrix tenuis Cultivation – a Cyanobacterium of Biotechnological Potential
Here I investigated the course of biomass growth, phosphorus uptake and changes in intracellular phosphorus content in T. tenuis in a batch cultivation. Cultivation experiment was held in multiple ...
Influence of Material Properties and Mixing Dynamics on Heat Transport in Granular Materials
Kokavcová, Anna
2024 - English
This study evaluates the effect of rotational speed on heat transfer and analyzes particle motion at different stirrer rotational frequencies to understand how primary and secondary flows affect heat transfer efficiency. It also examines the effect of Young’s modulus on heat transfer at different agitator\noperating speeds. Key simulation parameters and their influence 33 on heat transfer efficiency are detailed to improve the reproducibility and applicability of the results.
Keywords:
granular mixing; heat transfer; DEM
Available in digital repository of the ASCR
Influence of Material Properties and Mixing Dynamics on Heat Transport in Granular Materials
This study evaluates the effect of rotational speed on heat transfer and analyzes particle motion at different stirrer rotational frequencies to understand how primary and secondary flows affect heat ...
Assessment of the Presence of Hazardous Components in Textile Waste
Shtukaturova, Anastasia
2024 - English
An up-flow percolation test was chosen to identify and characterize heavy metals and PFAS constituents in textile waste. Up-flow column percolation tests are used on a laboratory scale to assess the leaching behavior of a hazardous substance from a waste in a given state as a function of time. The test was performed according to the European Technical Standard CEN/TS 14405. Preliminary results showed the following heavy metals in the leachates Al, As, Co, Cr, Cu, Fe, Mn, Pb, Zn and Sb, with Al, Cr, Fe, Mn and Zn being predominant.
Keywords:
textile waste; percolation test; PFAS; heavy metals
Available in a digital repository NRGL
Assessment of the Presence of Hazardous Components in Textile Waste
An up-flow percolation test was chosen to identify and characterize heavy metals and PFAS constituents in textile waste. Up-flow column percolation tests are used on a laboratory scale to assess the ...
Drone Based Vertical Profiling of Black Carbon Aerosols at a Rural Background and an Urban Site in Central Europe
Julaha, Kajal
2024 - English
This research aims to assess the vertical distribution of BC aerosols using a drone and micro Aethalometer AE51 at two distinct sites in the Czech Republic: 1. NAOK (National Atmospheric Observatory Košetice) representing a regional background, and 2. MFF (Faculty of Mathematics and Physics in Prague) representing an urban site. At NAOK, a 250-meter measurement tower is accessible, providing a means to validate and calibrate the drone measurements.
Available in a digital repository NRGL
Drone Based Vertical Profiling of Black Carbon Aerosols at a Rural Background and an Urban Site in Central Europe
This research aims to assess the vertical distribution of BC aerosols using a drone and micro Aethalometer AE51 at two distinct sites in the Czech Republic: 1. NAOK (National Atmospheric Observatory ...
Vertical Gradients of Atmospheric Aerosols Chemical Composition
Kovářík, Jiří
2024 - English
Concentration of chemical species chosen as markers for AA source apportionment are evaluated with regard to atmospheric conditions, seasonal and daily cycles and compared between the two heights. This work adds to the current state of general knowledge about AACC.
Keywords:
vertical gradients; chemical composition; atmospheric aerosols
Available in a digital repository NRGL
Vertical Gradients of Atmospheric Aerosols Chemical Composition
Concentration of chemical species chosen as markers for AA source apportionment are evaluated with regard to atmospheric conditions, seasonal and daily cycles and compared between the two heights. ...
Cultivation of Limnospira Maxima and Utilization of its Fresh Biomass in Human Nutrition
Vásquez, Claudia
2024 - English
This project aims to fill this gap by presenting new insights into its consumption as an alternative to dried spirulina and offering recommendations\nfor safe storage.
Keywords:
Limnospira maxima; dietary supplements; bioengineering
Available in a digital repository NRGL
Cultivation of Limnospira Maxima and Utilization of its Fresh Biomass in Human Nutrition
This project aims to fill this gap by presenting new insights into its consumption as an alternative to dried spirulina and offering recommendations\nfor safe storage.
Temoporfin-conjugated upconversion nanoparticles for NIR-induced photodynamic therapy of pancreatic cancer
Shapoval, Oleksandr; Větvička, D.; Kabešová, M.; Engstová, Hana; Horák, Daniel
2024 - English
Photodynamic therapy (PDT), a clinically approved cancer treatment strategy, has the potential to cure pancreatic cancer with minimal side effects. PDT primarily uses visible wavelengths to directly activate hydrophobic photosensitizers, which may be insufficient for deep-seated cancer cells in clinical practice due to poor penetration. Upconversion nanoparticles (UCNPs) serve as an indirect excitation source to activate photosensitizers (PSs) in the NIR region, overcoming the limitations of molecular PSs such as hydrophobicity, non-specificity, and excitation in the UV/Vis region. Here, monodisperse upconversion NaYF4:Yb3+, Er3+, Fe2+ nanoparticles (UCNPs) have been surface-engineered with poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) and temoporfin (mTHPC), a clinically used PDT prodrug, for near-infrared (NIR) light-triggered PDT of pancreatic cancer. The incorporation of Fe2+ ions into the particles increased the fluorescence intensity in the red region matching the activation wavelength of mTHPC. Covalent binding of mTHPC to the surface of UCNP@PMVEMA particles provided colloidally stable conjugates enabling generation of singlet oxygen. In vitro cytotoxicity and photodynamic activity of the particles were evaluated using INS-1E rat insulinoma and Capan-2 and PANC-01 human pancreatic adenocarcinoma cell lines. The PDT efficacy of UCNP@PMVEMA-mTHPC conjugates after irradiation with 980 nm NIR light was tested in vivo in a pilot study on Capan-2 human pancreatic adenocarcinoma growing subcutaneously in athymic nude mice. The intratumoral administration of the nanoconjugates significantly hindered tumor growth and demonstrated promising PDT efficacy against human pancreatic cancer.
Keywords:
photodynamic therapy; temoporfin; upconversion nanoparticles
Available in digital repository of the ASCR
Temoporfin-conjugated upconversion nanoparticles for NIR-induced photodynamic therapy of pancreatic cancer
Photodynamic therapy (PDT), a clinically approved cancer treatment strategy, has the potential to cure pancreatic cancer with minimal side effects. PDT primarily uses visible wavelengths to directly ...
Multi-Annual Characterization of PM1 Aerosol Optical Properties and Size Distribution at the Peri-Urban Atmospheric Site ATOLL in Lille
Suchánková, Lenka
2024 - English
The aim of this study is to present aerosol scattering, absorption properties and particle number size distribution in PM1 in years 2018-\n2022 at peri-urban atmospheric site Atmospheric Observations in LiLLE (ATOLL) in Lille, France. This study is the first to present results dedicated to aerosol scattering properties at this site.
Keywords:
aerosol particles; PM1; temporal trends
Available in digital repository of the ASCR
Multi-Annual Characterization of PM1 Aerosol Optical Properties and Size Distribution at the Peri-Urban Atmospheric Site ATOLL in Lille
The aim of this study is to present aerosol scattering, absorption properties and particle number size distribution in PM1 in years 2018-\n2022 at peri-urban atmospheric site Atmospheric Observations ...
Development of Continuous Technology for the Preparation of Pharmaceuticals
Jaklová, Natalie
2024 - English
The present research focuses on the transition of a batch reaction assisted by phase transfer catalyst (PTC) into continuous process. The first reaction studied was an N-alkylation reaction with methyl ester of the 1-methyl-10α-methoxydihydrolysergic acid (MeLUME) as product. MeLUME is one of the intermediates in synthesis of drug Nicergoline. The N-alkylation occurs in two different immiscible phases and the bridge between these phases is the phase transfer catalyst. The study was performed in several different continuous flow apparatuses. The best results were obtained in a capillary reactor, with assistance of ultrasound.
Keywords:
continualization; ultrasound; pharmaceuticals
Available in a digital repository NRGL
Development of Continuous Technology for the Preparation of Pharmaceuticals
The present research focuses on the transition of a batch reaction assisted by phase transfer catalyst (PTC) into continuous process. The first reaction studied was an N-alkylation reaction with ...
Bubble Breakup in Triton Solutions
Semlerová, Tereza
2024 - English
This work deals with investigation of the bubble breakup in surfactant Triton X-45 solutions and the results are compared with data measured in water and α-terpineol solutions as another type of surfactants.
Keywords:
bubble breakup; surfactants; dynamic surface tension
Available in digital repository of the ASCR
Bubble Breakup in Triton Solutions
This work deals with investigation of the bubble breakup in surfactant Triton X-45 solutions and the results are compared with data measured in water and α-terpineol solutions as another type of ...
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