No (83)
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Antitrophoblast cell-mediated immunity /AT-CMI/ in recurrent miscarriage and ectopic pregnacy
Madar, J.; Kučera, E.; Šůla, K.; Chaloupková, A.; Pěknicová, Jana; Tolarová, M.; Nouza, K.; Kinský, R.
2003 - English
Specific mechanisms operate in decidua nad trophoblast to establish and maintain immunological tolerance of the embryo in mother's uterus. due to the lack of this tolerance, disturbances of pregnancy may result. In this presentation, we have tested the hypothesis that anti-trohhoblast cell-mediated immunity (AT-CMI) is elevated in habitually aborting women and that extra-uterine localisation of the embryo leads to subsequent elevation of AT-CMI.
Keywords:
trophoblast; cardiolipin
Available at various institutes of the ASCR
Antitrophoblast cell-mediated immunity /AT-CMI/ in recurrent miscarriage and ectopic pregnacy
Specific mechanisms operate in decidua nad trophoblast to establish and maintain immunological tolerance of the embryo in mother's uterus. due to the lack of this tolerance, disturbances of pregnancy ...
Multigenerational study - effect of the selected compounds on the mouse fertility
Boubelík, Michael; Pěknicová, Jana; Kyselová, Vendula; Buckiová, D.
2003 - English
Several compounds known to be effective for their influence onto the environment were tested. Compounds tested in this study were: benzo/a/ pyrene, lindane (hexachlorocyclohexane isomer), 4-n-nonylphenol, bisphenol A and octachlorostyrene. The question was if these compounds might also function as endocrine disruptors.
Keywords:
endocrine disruptors; sperm motility
Available at various institutes of the ASCR
Multigenerational study - effect of the selected compounds on the mouse fertility
Several compounds known to be effective for their influence onto the environment were tested. Compounds tested in this study were: benzo/a/ pyrene, lindane (hexachlorocyclohexane isomer), ...
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
Fučík, Vladimír; Šindelka, Radek; Beran, Jaroslav; Ferjentsik, Zoltán; Jonák, Jiří
2003 - English
In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in specific intervals. As the 31 bp interlink can assume a hairpin structure, the whole region can be viewed as an imperfect inverted repeat. The microsatellite was sequenced in imbred lines of M. m. musculus and M. m. domesticus, as well as in mice of both subspecies trapped near the hybrid zone. Differences in the length of repeat regions, insertion/deletions and simple base changes characterize the strains. Hybrids between the two species could be identified by sequencing of cloned PCR products. The structure of the microsatellite in more distant species was found to be conspicuously different. Instead of frequent (CA)18-25, we can see only (CA)8 in M. spretus, CATGCGCCCCCCA in Mus pahari and CACCCCCC in Mus caroli. No similar structure is present in the corresponding human src intron.
Keywords:
Mus musculus, c-src introns, microsatellite
Available at various institutes of the ASCR
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution
In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in ...
A Phenylnorstatine Inhibitor Binding to HIV-1 Protease: Geometry, Protonation and Subsite-Pocket Interactions Analyzed at Atomic Resolution
Brynda, Jiří; Řezáčová, Pavlína; Fábry, Milan; Hořejší, Magdalena; Štouračová, Renata; Sedláček, Juraj; Souček, M.; Hradílek, M.; Lepšík, M.; Konvalinka, J.
2003 - English
The x-ray structure of a complex of HIV-1 protease (PR) with a phenylnorstatine inhibitor Z-Pns-Phe-Glu-Glu-NH2 has been determined at 1.03 A, the highest resolution so far reported for any HIV PR complex. The inhibiot shows subnanomolar Ki values for both the wild-type PR and the variant representing one of the most common mutations linked to resistance developoment. The structure displays a unique pattern of hydrogen bonding to the two catalytic aspartate residues. The high resolution permits to assess the donor/acceptor realtions of this hydrogen bonding and to indicate a proton shared by the two catalytic residues. Structural mechanism for the unimpaired ihnibition of the protease Val82Ala mutant is also suggested, based on energy calculations and analyses.
Keywords:
inhibitor; HIV protease; atomic resolution
Available at various institutes of the ASCR
A Phenylnorstatine Inhibitor Binding to HIV-1 Protease: Geometry, Protonation and Subsite-Pocket Interactions Analyzed at Atomic Resolution
The x-ray structure of a complex of HIV-1 protease (PR) with a phenylnorstatine inhibitor Z-Pns-Phe-Glu-Glu-NH2 has been determined at 1.03 A, the highest resolution so far reported for any HIV PR ...
Metody experimentálního určení fází (MIR, MAD,SAD) v krystalografické analýze biologických makromolekul
Brynda, Jiří
2003 - Czech
Důležitost metod poskytujících nepředpojaté řešení fázového problému se stává vstupem molekulární biologie a biochemie do tzv. post-genomické éry stále významnější. V článku jsou popsány klasické metody experimentálního určení fází:1) Metoda isomorfních derivátů s těžkými atomy 2) Metoda Anomálního rozptylu při různých vlnových délkách 3) Metoda Anomálního rozptylu při jedné vlnové délce Čím lepší jsou vaše první, experimentální mapy, tím více modelu můžete spolehlivě vystavět do nepředpojaté (modelem nezatížené) mapy před sečtením fází (původní + model). MAD fáze pocházejí z perfektně izomorfních dat a tak netrpí disordrem jako je to běžné u MIR map na malém rozlišení. MIR fáze zase na druhou stranu poskytují výtečnou fázovací sílu na nízkém rozlišení a výsledkem je lepší propojení mapy v prostoru. Není proto překvapivé, že nejlepší mapy jsou buď MAD mapy nebo kombinované MIR-MAD mapy. Importance of unbiased phases in post genomic era is emphasized. Article describes the classical methods of phase determination: 1) Multiple Isomorphous replacement, heavy-atom derivative (MIR) 2) Multiple Anomalous Dispersion (MAD) Phasing 3) Single Anomalous Dispersion (SAD) Phasing The better your initial, experimental map is, the more model you will be able to build confidently into this unbiased map before proceeding to phase combination. MAD phases result from perfectly isomorphous data, and do not deteriorate at low resolution as do MIR phases. MIR phases, on the other hand, provide superior phasing power at low resolution resulting in better connectivity. Not surprising, the best maps are either MAD or combined MIR-MAD maps.
Keywords:
phase problem; MIR,MAD,SAD
Available at various institutes of the ASCR
Metody experimentálního určení fází (MIR, MAD,SAD) v krystalografické analýze biologických makromolekul
Důležitost metod poskytujících nepředpojaté řešení fázového problému se stává vstupem molekulární biologie a biochemie do tzv. post-genomické éry stále významnější. V článku jsou popsány klasické ...
Crystallographic study of an anti=carbonic anhydrase IX monoclonal antibody M75
Štouračová, Renata; Závada, Jan; Závadová, Zuzana; Pastoreková, S.; Brynda, Jiří; Fábry, Milan; Král, Vlastimil; Hořejší, Magdalena; Sedláček, Juraj
2003 - English
Carbonic anhydrase IX (CA IX) is a cell surface protein, strongly associated with certain types of human carcinomas. Structural study of a CA IX-binding monoclonal antibody (mAb) M75, complexed with its epitope peptide may contribute toward elucidation of the role of CA IX. Monoclonal antibody M75 was obtained and proved to react excellently with native and denaturated CA IX. Using synthetic oligopeptides, the epitope of mAb M75 was localized in the proteoglycan domain of CA IX, in the region of a tandem repeat and identified as amino acids PGEEDLP. The Fab fragment was obtained by papain cleavage. We obtained crystals of free Fab M75 and Fab M75 complexed with two different epitope peptides. The data set for Fab M75 was collected and the structure solving is underway.
Keywords:
anti-carbonic ahydrase antibody, cancer, crystallization
Available at various institutes of the ASCR
Crystallographic study of an anti=carbonic anhydrase IX monoclonal antibody M75
Carbonic anhydrase IX (CA IX) is a cell surface protein, strongly associated with certain types of human carcinomas. Structural study of a CA IX-binding monoclonal antibody (mAb) M75, complexed with ...
Structural basis of HIV-1 and HIV-2 protease inhibition bya monoclonal antibody
Řezáčová, Pavlína; Lescar, J.; Brynda, Jiří; Fábry, Milan; Bentley, G. A.; Sedláček, Juraj
2003 - English
The murine monoclonal antibody 1696, produced by immunisation with the HIV-1 protease,inhibits the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates, with inhibitionconstants in the low nanomolar range. This antibody cross-reacts with peptides that include theN-terminus of the enzyme (residues 1-7), a region which is highly conserved in sequence amongdifferent viral strains and which, furthermore, is crucial for homodimerization to the activeenzymatic form.We report here two crystal structures of a recombinant single-chain Fv fragment of mAb 1696,expressed in E. coli, as a complex with a cross-reactive peptides from the HIV-1 PR and theHIV-2 PR at 2.7 ? resolution and 1.9 ? resolution respectively. On the basis of the interactionsseen in the complex three-dimensional structures, the cross-reactivity between mAb 1696 withthe HIV-1 and HIV-2 protease and their N-terminal peptides can be explained. In addition, acandidate mechanism of HIV PR inhibition by mAb 1696 is proposed which may help thedesign of alternative HIV protease inhibitors, aimed at dissociating the homodimeric viral enzyme.
Keywords:
antibody fragment; crystal structure; HIV protease inhibition
Available at various institutes of the ASCR
Structural basis of HIV-1 and HIV-2 protease inhibition bya monoclonal antibody
The murine monoclonal antibody 1696, produced by immunisation with the HIV-1 protease,inhibits the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates, with inhibitionconstants in ...
ISOLATION AND CHARACTERIZATION OF MANNAN-BINDING PROTEINS FROM BOAR SEMINAL PLASMA
Jelínková, Petra; Liberda, J.; Ryšlavá, H.; Tichá, M.; Jonáková, Věra
2003 - English
The mannan-binding proteins of boar seminal plasma may be involved in the formation of the sperm oviductal reservoir in pig. The ability of these proteins to interact both with the complex of D-mannose-containing saccharide structures and heparin may also play an important role in the sperm release from the oviductal reservoir and in the capacitation process.
Keywords:
mannan-binding proteins; oviductal reservoir; boar seminal plasma
Available at various institutes of the ASCR
ISOLATION AND CHARACTERIZATION OF MANNAN-BINDING PROTEINS FROM BOAR SEMINAL PLASMA
The mannan-binding proteins of boar seminal plasma may be involved in the formation of the sperm oviductal reservoir in pig. The ability of these proteins to interact both with the complex of ...
Significance of the acrosome reaction and sperm agglutinating antibodies in human semen. acrosome RE
Pavlásek, J.; Pěknicová, Jana; Ulčová-Galová, Z.; Nováková, P.; Mičanová, Z.; Rokyta, Z.
2003 - English
We commpared results of the acrosome reaction and sperm agglutinating antibodies in human semen samples.
Keywords:
acrosome reaction; monoclonal antibody
Available at various institutes of the ASCR
Significance of the acrosome reaction and sperm agglutinating antibodies in human semen. acrosome RE
We commpared results of the acrosome reaction and sperm agglutinating antibodies in human semen samples.
Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Jelínková, Petra; Tichá, M.; Jonáková, Věra
2003 - English
Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present communication we have shown that serine proteinase inhibitors participate (under physiological conditions) in the formation of associated complexes with other proteins from boar seminal plasma.
Keywords:
boar seminal plasma proteins; proteinase inhibitors
Available at various institutes of the ASCR
Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present ...
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