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Bioluminescent probe for caspase imaging inside cells based on Foerster resonance energy transfer between quantum dot and quencher
Procházková, Markéta; Klepárník, Karel
2021 - English
In this work testing of novel quantum dot luminescent probe is presented. The quantum dot FRET-based luminescent probe enables long-time luminescent imaging of active caspases in living cells under microscope.
Keywords:
caspase; fluorescence; quantum dot
Available in digital repository of the ASCR
Bioluminescent probe for caspase imaging inside cells based on Foerster resonance energy transfer between quantum dot and quencher
In this work testing of novel quantum dot luminescent probe is presented. The quantum dot FRET-based luminescent probe enables long-time luminescent imaging of active caspases in living cells under ...
Novel photochemical vapor generation of rhenium for its ultratrace determination
Vyhnanovský, Jaromír; Hašlová, K.; Musil, Stanislav
2021 - English
The presentation focused on novel photochemical vapor generation of rhenium. Details on optimization of generation conditions and other important parameters regarding this sensitive methodology, as well as its possible applications were pointed out and discussed.
Keywords:
photochemical vapor generation; inductively coupled plasma mass spectrometry; rhenium
Available in digital repository of the ASCR
Novel photochemical vapor generation of rhenium for its ultratrace determination
The presentation focused on novel photochemical vapor generation of rhenium. Details on optimization of generation conditions and other important parameters regarding this sensitive methodology, as ...
Nanospray processing of silver nanoparticles for formation of dried deposits
Týčová, Anna; Jonas, Vladimír; Přikryl, Jan; Kotzianová, A.; Velebný, V.; Foret, František
2021 - English
Nanospray transfers liquid into an aerosol via electrostatic forces created between an emitter and a counter electrode. Herein, we present compact laboratory-made instrumentation for nanospray-processing of silver colloid. The instrumentation is based on the pressurized polysulfone chamber fixing a disposable vial with an immersed platinum electrode and a long fused silica capillary supplying the sprayed liquid to the emitter. The combination of low flow rates (70 nL/min) with a sharp emitter, fabricated on a 3D printed grinding station, resulted in a fine aerosol. The low volume of released droplets allowed full evaporation of water during their flight towards the counter electrode without any need for drying gas or the addition of volatile solvents. The constructed device was successfully used for the deposition of water-free silver colloid without any requirement of its pretreatment. The deposition of completely dried nanoparticles on planar substrates eliminated undesirable coffee ring effect and deposits of increased homogeneity could be obtained. Electron microscopy confirmed no significant changes in the character of nanospray-processed nanoparticles. Finally, we also investigated several approaches for the improvement of the surface density of nanoparticles on the substrate at preserved time scale.
Keywords:
nanoparticles; nanospray; silver
Available in digital repository of the ASCR
Nanospray processing of silver nanoparticles for formation of dried deposits
Nanospray transfers liquid into an aerosol via electrostatic forces created between an emitter and a counter electrode. Herein, we present compact laboratory-made instrumentation for ...
Optimization of photochemical vapor generation of tellurium for analytical atomic spectrometry
Jeníková, Eva; Musil, Stanislav; Hraníček, J.
2021 - English
Photochemical vapor generation of tellurium was studied using a miniature diffusion flame atomizer and high-resolution continuum source atomic absorption spectrometry.
Keywords:
tellurium photochemical vapor generation; atomic absorption spectrometry
Available in digital repository of the ASCR
Optimization of photochemical vapor generation of tellurium for analytical atomic spectrometry
Photochemical vapor generation of tellurium was studied using a miniature diffusion flame atomizer and high-resolution continuum source atomic absorption spectrometry.
Comparison of radiation sources for atomic fluorescence spectrometry
Štádlerová, Barbora; Dědina, Jiří; Musil, Stanislav
2021 - English
Comparison of a bismuth boost-discharge hollow cathode lamp and and electrodeless discharge lamp employed as an excitation source in an atomic fluorescence spectrometer.
Keywords:
atomic fluorescence spectrometry; bismuth; hydride generation
Available in digital repository of the ASCR
Comparison of radiation sources for atomic fluorescence spectrometry
Comparison of a bismuth boost-discharge hollow cathode lamp and and electrodeless discharge lamp employed as an excitation source in an atomic fluorescence spectrometer.
The effect of milk thistle cultivation technology [Silybum marianum (L.) Gaertner] on the yield and contained compounds
Fojtíková, L.; Bradáčová, M.; Kudláčková, Barbora; Bjelková, M.; Pluháčková, H.
2021 - English
The aim of the present study was to evaluate the impact of complex growing technology on the quality of milk thistle achenes.
Keywords:
milk thistle; cultivation technology; silymarin complex
Fulltext is available at external website.
The effect of milk thistle cultivation technology [Silybum marianum (L.) Gaertner] on the yield and contained compounds
The aim of the present study was to evaluate the impact of complex growing technology on the quality of milk thistle achenes.
Chemical vapor generation of cadmium: detector comparison
Sagapova, Linda; Štádlerová, Barbora; Svoboda, Milan; Kratzer, Jan; Musil, Stanislav
2021 - English
A chemical vapor generation system was connected to an in-house assembled atomic fluorescence spectrometer (AFS) equipped with a miniature diffusion flame atomizer in order to assess its potential to Cd determination. Subsequently, analytical performance of three spectrometric detectors was compared (AAS, AFS, ICP-MS) with respect to Cd determination after volatile species generation.
Keywords:
chemical vapor generation; cadmium; atomic fluorescence spectrometry
Available in digital repository of the ASCR
Chemical vapor generation of cadmium: detector comparison
A chemical vapor generation system was connected to an in-house assembled atomic fluorescence spectrometer (AFS) equipped with a miniature diffusion flame atomizer in order to assess its potential to ...
Bioluminescence determination of caspase-3/7 in single osteosarcoma cells
Killinger, Michael; Matalová, Eva; Klepárník, Karel
2021 - English
The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate quantity of relevant proteins at cellular level is essential for appropriate interpretation of diagnostic and therapeutic results. Here, we determined the level of active caspase-3/7 in physiological and pathological (cancer) conditions by our miniaturized instrumentation.
Keywords:
single-cell analysis; osteoblast; osteosarcoma
Available in digital repository of the ASCR
Bioluminescence determination of caspase-3/7 in single osteosarcoma cells
The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate ...
Epitachophoretic preconcentration of proteins
Hrušková, Helena; Voráčová, Ivona; Foret, František
2021 - English
In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide low analysis time, narrow shape of the protein zone, and eliminate overheating at the center of the device. In the next step, the developed method was tested on the preconcentration of proteins: cytochrome c, myoglobin, and hemoglobin. After several adjustments myoglobin and hemoglobin were also uccessfully preconcentrated. After evaluation of pilot results, this technique provides up to fiftyfold preconcentration and recovery of over 70 % for each protein. The next plan is to preconcentrate proteins from complex biological matrices such as urine or blood plasma.
Keywords:
epitachophoresis; isotachophoresis; preparative concentration
Available on request at various institutes of the ASCR
Epitachophoretic preconcentration of proteins
In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide ...
Quantum dot luminescent probe for Caspase 3/7 imaging inside cells
Procházková, Markéta; Klepárník, Karel
2021 - English
Two step synthesis of luminescent probe based on ligand-exchange in the first step and the specific reaction of amino group in the second step was optimized. The luminescence properties of the final product were checked by time course of\nactive recombinant caspase protein reaction under the model conditions in fluorimeter. The novel luminescent probe enables long-time imaging of active caspases in living cells or tissues.
Keywords:
caspase; fluorescence; quantum dot
Available on request at various institutes of the ASCR
Quantum dot luminescent probe for Caspase 3/7 imaging inside cells
Two step synthesis of luminescent probe based on ligand-exchange in the first step and the specific reaction of amino group in the second step was optimized. The luminescence properties of the final ...
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