Crystallographic study of an anti=carbonic anhydrase IX monoclonal antibody M75
Štouračová, Renata; Závada, Jan; Závadová, Zuzana; Pastoreková, S.; Brynda, Jiří; Fábry, Milan; Král, Vlastimil; Hořejší, Magdalena; Sedláček, Juraj
2003 - English
Carbonic anhydrase IX (CA IX) is a cell surface protein, strongly associated with certain types of human carcinomas. Structural study of a CA IX-binding monoclonal antibody (mAb) M75, complexed with its epitope peptide may contribute toward elucidation of the role of CA IX. Monoclonal antibody M75 was obtained and proved to react excellently with native and denaturated CA IX. Using synthetic oligopeptides, the epitope of mAb M75 was localized in the proteoglycan domain of CA IX, in the region of a tandem repeat and identified as amino acids PGEEDLP. The Fab fragment was obtained by papain cleavage. We obtained crystals of free Fab M75 and Fab M75 complexed with two different epitope peptides. The data set for Fab M75 was collected and the structure solving is underway.
Keywords:
anti-carbonic ahydrase antibody, cancer, crystallization
Available at various institutes of the ASCR
Crystallographic study of an anti=carbonic anhydrase IX monoclonal antibody M75
Carbonic anhydrase IX (CA IX) is a cell surface protein, strongly associated with certain types of human carcinomas. Structural study of a CA IX-binding monoclonal antibody (mAb) M75, complexed with ...
Structural basis of HIV-1 and HIV-2 protease inhibition bya monoclonal antibody
Řezáčová, Pavlína; Lescar, J.; Brynda, Jiří; Fábry, Milan; Bentley, G. A.; Sedláček, Juraj
2003 - English
The murine monoclonal antibody 1696, produced by immunisation with the HIV-1 protease,inhibits the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates, with inhibitionconstants in the low nanomolar range. This antibody cross-reacts with peptides that include theN-terminus of the enzyme (residues 1-7), a region which is highly conserved in sequence amongdifferent viral strains and which, furthermore, is crucial for homodimerization to the activeenzymatic form.We report here two crystal structures of a recombinant single-chain Fv fragment of mAb 1696,expressed in E. coli, as a complex with a cross-reactive peptides from the HIV-1 PR and theHIV-2 PR at 2.7 ? resolution and 1.9 ? resolution respectively. On the basis of the interactionsseen in the complex three-dimensional structures, the cross-reactivity between mAb 1696 withthe HIV-1 and HIV-2 protease and their N-terminal peptides can be explained. In addition, acandidate mechanism of HIV PR inhibition by mAb 1696 is proposed which may help thedesign of alternative HIV protease inhibitors, aimed at dissociating the homodimeric viral enzyme.
Keywords:
antibody fragment; crystal structure; HIV protease inhibition
Available at various institutes of the ASCR
Structural basis of HIV-1 and HIV-2 protease inhibition bya monoclonal antibody
The murine monoclonal antibody 1696, produced by immunisation with the HIV-1 protease,inhibits the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates, with inhibitionconstants in ...
ISOLATION AND CHARACTERIZATION OF MANNAN-BINDING PROTEINS FROM BOAR SEMINAL PLASMA
Jelínková, Petra; Liberda, J.; Ryšlavá, H.; Tichá, M.; Jonáková, Věra
2003 - English
The mannan-binding proteins of boar seminal plasma may be involved in the formation of the sperm oviductal reservoir in pig. The ability of these proteins to interact both with the complex of D-mannose-containing saccharide structures and heparin may also play an important role in the sperm release from the oviductal reservoir and in the capacitation process.
Keywords:
mannan-binding proteins; oviductal reservoir; boar seminal plasma
Available at various institutes of the ASCR
ISOLATION AND CHARACTERIZATION OF MANNAN-BINDING PROTEINS FROM BOAR SEMINAL PLASMA
The mannan-binding proteins of boar seminal plasma may be involved in the formation of the sperm oviductal reservoir in pig. The ability of these proteins to interact both with the complex of ...
Significance of the acrosome reaction and sperm agglutinating antibodies in human semen. acrosome RE
Pavlásek, J.; Pěknicová, Jana; Ulčová-Galová, Z.; Nováková, P.; Mičanová, Z.; Rokyta, Z.
2003 - English
We commpared results of the acrosome reaction and sperm agglutinating antibodies in human semen samples.
Keywords:
acrosome reaction; monoclonal antibody
Available at various institutes of the ASCR
Significance of the acrosome reaction and sperm agglutinating antibodies in human semen. acrosome RE
We commpared results of the acrosome reaction and sperm agglutinating antibodies in human semen samples.
Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Jelínková, Petra; Tichá, M.; Jonáková, Věra
2003 - English
Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present communication we have shown that serine proteinase inhibitors participate (under physiological conditions) in the formation of associated complexes with other proteins from boar seminal plasma.
Keywords:
boar seminal plasma proteins; proteinase inhibitors
Available at various institutes of the ASCR
Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present ...
Protein Characterization of Boar Epididymal Fluid
Maňásková, Pavla; Tichá, M.; Jonáková, Věra
2003 - English
Keywords:
epididymal maturation, proteins, fertilization
Available at various institutes of the ASCR
Protein Characterization of Boar Epididymal Fluid
Role of sperm surface proteins in gamete binding
Jonáková, Věra; Tichá, M.
2003 - English
Mammalian fertilization includes highly regulated biochemical interactions between complementary molecules located on the surface of both gametes. Sperm surface proteins play various roles in the fertilization process.
Keywords:
boar seminal plasma proteins, gamete binding, fertilization
Available at various institutes of the ASCR
Role of sperm surface proteins in gamete binding
Mammalian fertilization includes highly regulated biochemical interactions between complementary molecules located on the surface of both gametes. Sperm surface proteins play various roles in the ...
Study of sperm proteins in modelspecies of chondrostean and teleostean fish
Koubek, Pavel; Elzeinová, Fatima; Linhart, O.; Pěknicová, Jana
2003 - English
Specific in the study of fish reproduction is outer fertilization. spermatozoa are usually expelled into host medium of the outer encironment. The cells have to cope with an extremely different encironment and their protection against the outer environment is much weaker than that of oocytes. The way of fertilization also differs from mammalian fertilization.
Keywords:
cyprinus; carpio; acipenser
Available at various institutes of the ASCR
Study of sperm proteins in modelspecies of chondrostean and teleostean fish
Specific in the study of fish reproduction is outer fertilization. spermatozoa are usually expelled into host medium of the outer encironment. The cells have to cope with an extremely different ...
Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J.; Dvořáková, K.; Pěknicová, Jana; Kubátová, Alena; Moore, H. D. M.
2003 - English
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes.
Keywords:
cytoskeleton; spermatozoa
Available at various institutes of the ASCR
Dynamic and role of cytosekeletal proteins in mammalian sperm cells
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor ...
noTitle
Teplá, O.; Pěknicová, Jana; Mrázek, M.; Kočí, K.; Chládek, David; Míka, M.; Badalová, J.; Pecka, T.; Fulka, F.
2003 - English
Iscare IVF a.s. had a long record of successful azoospermic treatment since 1997, when the first pregnancies by MESA a TESE were published in the Czech republic. A series of diferent implanted laboratory techniques supported good results from last year. The medical problem is solved during the other field of application-histlogical findings and immunofluorescent methods.
Keywords:
MESA; azoospermia; TESE
Available at various institutes of the ASCR
noTitle
Iscare IVF a.s. had a long record of successful azoospermic treatment since 1997, when the first pregnancies by MESA a TESE were published in the Czech republic. A series of diferent implanted ...
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