Analýza doby letu nejen v hmotnostní spektrometrii
Cvačka, Josef
2016 - Czech
Byl vysvětlen princip a použití analyzátorů doby letu v hmotnostní spektrometrii a jiných oborech. Principles and application examples of time of flight analyzers in mass spectrometry and other fields were explained.
Keywords:
TOF; mass spectrometry
Available at various institutes of the ASCR
Analýza doby letu nejen v hmotnostní spektrometrii
Byl vysvětlen princip a použití analyzátorů doby letu v hmotnostní spektrometrii a jiných oborech....
Využití hmotnostní spektrometrie pro analýzu nepolárních sloučenin
Vrkoslav, Vladimír
2016 - Czech
Bylo popsáno využití hmotnostně spektrometrických technik pro detekci nepolárních sloučenin. Using of mass spectrometry techniques for detection of nonpolar compounds was described.
Keywords:
non-polar compounds; mass spectrometry
Available at various institutes of the ASCR
Využití hmotnostní spektrometrie pro analýzu nepolárních sloučenin
Bylo popsáno využití hmotnostně spektrometrických technik pro detekci nepolárních sloučenin.
Using of mass spectrometry techniques for detection of nonpolar compounds was described.
Capillary isotachophoresis and zone electrophoresis applied to determination of effective charge of polyelectrolytes
Kašička, Václav; Koval, Dušan; Ibrahim, A.; Chamieh, J.; Cottet, H.
2016 - English
Capillary electromigration methods, isotachophoresis (CITP) and zone electrophoresis (CZE) were applied to determination of effective charge of poly-alpha-L-glutamic acid and poly-alpha-L-lysine using both CITP-dedicated device and common CZE apparatus. Combination of CITP and CZE methods in both these types of devices proved to be a suitable platform for determination of effective charge of a wide range of polyelectrolytes – weak and strong, polycations and polyanions.
Keywords:
capillary isotachophoresis; polyelectrolytes; effective charge
Available at various institutes of the ASCR
Capillary isotachophoresis and zone electrophoresis applied to determination of effective charge of polyelectrolytes
Capillary electromigration methods, isotachophoresis (CITP) and zone electrophoresis (CZE) were applied to determination of effective charge of poly-alpha-L-glutamic acid and poly-alpha-L-lysine using ...
Determination of stability constants of [Gly6]-antamanide complexes with small cations by affinity capillary electrophoresis
Pangavhane, Sachin; Makrlík, E.; Ruzza, P.; Kašička, Václav
2016 - English
Affinity capillary electrophoresis (ACE) was employed for the determination of apparent stability constants Kst of complexes of cyclic peptide [Gly6]-antamanide ([Gly6]-AA) with alkali and alkali-earth metal ions: Li+, Na+, K+, Rb+, Cs+, NH4+, and Ca2+. Kst was determined from the dependence of effective mobility of [Gly6]-AA on the concentration of these ions in the background electrolyte using the non-linear regression analysis. Kst were found to be in the range 13.3 – 26.3.
Keywords:
affinity capillary electrophoresis; [Gly6]-antamanide; stability constant
Available at various institutes of the ASCR
Determination of stability constants of [Gly6]-antamanide complexes with small cations by affinity capillary electrophoresis
Affinity capillary electrophoresis (ACE) was employed for the determination of apparent stability constants Kst of complexes of cyclic peptide [Gly6]-antamanide ([Gly6]-AA) with alkali and ...
Investigation of acid-based properties of mono- and diaza[5]helicenes by non-aqueous capillary electrophoresis and quantum chemical calculations
Šolínová, Veronika; Štěpánová, Sille; Jančařík, Andrej; Klívar, Jiří; Šámal, Michal; Stará, Irena G.; Vacek Chocholoušová, Jana; Vacek, Jaroslav; Starý, Ivo; Kašička, Václav
2016 - English
Non-aqueous capillary electrophoresis (NACE) using methanol (MeOH) as a solvent of the background electrolytes was used to determine the thermodynamic acidity (ionization) constants (pKa) of a series of monoaza[5]helicenes and diaza[5]helicenes protonated to the first degree in MeOH and water. Depending on the number and position of the nitrogen atoms in their molecules, the analyzed aza[5]helicenes were found to be weak to moderate bases with methanolic pKa,MeOH in the range 3.90-8.75 and with aqueous pKa,H2O in the range 3.53-8.28.
Keywords:
capillary electrophoresis; azahelicenes; acidity constant
Available at various institutes of the ASCR
Investigation of acid-based properties of mono- and diaza[5]helicenes by non-aqueous capillary electrophoresis and quantum chemical calculations
Non-aqueous capillary electrophoresis (NACE) using methanol (MeOH) as a solvent of the background electrolytes was used to determine the thermodynamic acidity (ionization) constants (pKa) of a series ...
Capillary electrophoretic separation and structure-mobility relationship study of cyclic antimicrobial peptides
Tůmová, Tereza; Monincová, Lenka; Čeřovský, Václav; Kašička, Václav
2016 - English
Capillary electrophoresis (CE) has been employed for qualitative and quantitative analysis, separation and physicochemical characterization of antimicrobial peptides isolated from the venom of eusocial bee Lassioglossum laticeps. From the CE experiments at various pH, the important physico-chemical parameters of analyzed peptides, effective mobilities and acidity constants were determined and the structure-mobility relationships of peptides with variable intramolecular cyclization of their molecules were investigated.
Keywords:
antimicrobial peptides; capillary electrophoresis; structure-mobility relationships
Available at various institutes of the ASCR
Capillary electrophoretic separation and structure-mobility relationship study of cyclic antimicrobial peptides
Capillary electrophoresis (CE) has been employed for qualitative and quantitative analysis, separation and physicochemical characterization of antimicrobial peptides isolated from the venom of ...
Mimikování metabolismu thiramu pomocí elektrochemie spojené s hmotnostní spektrometrií
Jaklová Dytrtová, Jana; Jakl, M.
2016 - Czech
On-line zapojení elektrochemické cely vlastní výroby před hmotnostní spektrometr s ionizací elektrosprejem (EC-ESI-MS) bylo studováno pro možné využití při simulaci metabolismu thiramu (T). Instrumentační přístup EC-ESI-MS potvrzuje vytvoření prvního metabolického produktu T (Tm). Tm se tvoří rozpadem disulfidové vazby. Je diskutován katalytický účinek mědi pocházející z měděné pracovní elektrody na tuto reakci. On-line hyphenation of a home-made electrochemical cell with an electrospray ionization mass spectrometer (EC-ESI-MS) was investigated to be applicable in mimicking of thiram (T) metabolism. The EC-ESI-MS instrumental approach confirms the creation of the first metabolic product of T (Tm). Tm is created via the decay of the disulfide bond. The catalytic effect of copper originated from the copper working electrode to this reaction is discussed.
Keywords:
cytochrome P450; oxidation; reduction; lab-on-chip; ex situ
Available at various institutes of the ASCR
Mimikování metabolismu thiramu pomocí elektrochemie spojené s hmotnostní spektrometrií
On-line zapojení elektrochemické cely vlastní výroby před hmotnostní spektrometr s ionizací elektrosprejem (EC-ESI-MS) bylo studováno pro možné využití při simulaci metabolismu thiramu (T). ...
Mobilization of neutral compounds by inorganic and small organic ions in capillary electrophoresis
Křížek, T.; Martínková, E.; Kubíčková, A.; Hladílková, Jana; Coufal, P.; Heyda, J.; Jungwirth, Pavel
2016 - English
The present study shows that neutral low-molecular-mass compounds that are presumed to possess zero electrophoretic mobility and are thus used as markers of electroosmotic flow in capillary electrophoresis can acquire electrophoretic mobility due to ion-specific interactions with constituents of background electrolyte. Therefore, these neutral markers, such as thiourea or dimethyl sulfoxide, can be separated in electric field and their migration order can vary depending on background electrolyte composition. This observation has some important implications for analytical and physico-chemical measurements using capillary electrophoresis because the choice of the marker-background electrolyte combination can influence the results of the measurement.
Keywords:
capillary electrophoresis; EOF markers; ion-specific effects; mobilization
Available at various institutes of the ASCR
Mobilization of neutral compounds by inorganic and small organic ions in capillary electrophoresis
The present study shows that neutral low-molecular-mass compounds that are presumed to possess zero electrophoretic mobility and are thus used as markers of electroosmotic flow in capillary ...
Investigation of calix[4]arene-bis(t-octylbenzo-18-crown-6) complex with cesium cation by affinity capillary electrophoresis
Štěpánová, Sille; Moyer, B.; Makrlík, E.; Kašička, Václav
2016 - English
Affinity capillary electrophoresis (ACE) was employed for investigation of non-covalent interactions of calix[4]arene-bis(t-octylbenzo-18-crown-6) with Cs+ ion in methanol. The apparent binding constant of the calix[4]arene-bis(t-octylbenzo-18-crown-6)-Cs+ complex was determined from the dependence of the effective electrophoretic mobilities of the calix[4]arene-bis(t-octylbenzo-18-crown-6) on the concentration of cesium ion in the backround electrolyte using a non-linear regression analysis. The logarithmic form of the conditional apparent binding constant of calix[4]arene-bis(t-octylbenzo-18-crown-6)-Cs+ complex was found to be equal to 1.61.
Keywords:
affinity capillary electrophoresis; calix[4]arene-bis(t-octylbenzo-18-crown-6); binding constant
Available at various institutes of the ASCR
Investigation of calix[4]arene-bis(t-octylbenzo-18-crown-6) complex with cesium cation by affinity capillary electrophoresis
Affinity capillary electrophoresis (ACE) was employed for investigation of non-covalent interactions of calix[4]arene-bis(t-octylbenzo-18-crown-6) with Cs+ ion in methanol. The apparent binding ...
Determination of stability constant of potassium-dibenzo-18-crown-6 complex in mixed hydro-organic solvents by affinity capillary electrophoresis
Konášová, Renáta; Jaklová Dytrtová, Jana; Kašička, Václav
2016 - English
Affinity capillary electrophoresis was applied to determination of apparent stability constant, Kst, of potassium-dibenzo-18-crown-6 complex in mixed hydro-organic solvents, i.e. mixtures of water with methanol, ethanol, propan-2-ol or acetonitrile. It was found that the Kst strongly depends on the solvent composition. The log Kst values linearly increase with decreasing molar fraction of water in the above hydro-organic solvent mixture.
Keywords:
affinity capillary electrophoresis; dibenzo-18-crown-6; stability constant
Available at various institutes of the ASCR
Determination of stability constant of potassium-dibenzo-18-crown-6 complex in mixed hydro-organic solvents by affinity capillary electrophoresis
Affinity capillary electrophoresis was applied to determination of apparent stability constant, Kst, of potassium-dibenzo-18-crown-6 complex in mixed hydro-organic solvents, i.e. mixtures of water ...
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