Genetic and epigenetic acpects of sex determination
Janoušek, Bohuslav; Hodurková, Jaromíra; Žlůvová, Jitka; Lengerová, Martina; Hobza, Roman; Nix, Tomáš; Vyskot, Boris
2002 - English
The article concentrates on the latest knowledge concerning the role of epigenetic mechanisms in sex determination in model dioecious species Silene latifolia. Results suggest that the locus suppressing development of female organs in male plants is hypersensitive to the action of agents influencing level of DNA methylation (5-azacytidine) and histone acetylation (trichostatin A). According to the results obtained via inter-specific hybridisation and the ability of the smut Ustilago violacea to activate development of male organs in female plants, it can be deduced that there is a locus suppressing male organ development in standard female plants. Function of this locus is not influenced by the drugs mentioned above.
Keywords:
Silene latifolia; Ustilago violacea; sex determination
Available at various institutes of the ASCR
Genetic and epigenetic acpects of sex determination
The article concentrates on the latest knowledge concerning the role of epigenetic mechanisms in sex determination in model dioecious species Silene latifolia. Results suggest that the locus ...
Impedance measurements of nucleic acids and self-assembled layers of their components
Vetterl, Vladimír; Jelen, František; Dražan, Viktor; Strašák, Luděk; Hasoň, Stanislav; Dvořák, Jakub
2002 - English
The kinetics of structural changes of cytidine adlayers on the mercury electrodes were investigated by means of current and capacitance transients using the potential step technique.
Keywords:
nucleic acids; electochemical impedance; mercury electrodes
Available at various institutes of the ASCR
Impedance measurements of nucleic acids and self-assembled layers of their components
The kinetics of structural changes of cytidine adlayers on the mercury electrodes were investigated by means of current and capacitance transients using the potential step technique.
Structure and evolution of plant sex chromosomes: The use of BAC library
Kejnovský, Eduard; Lengerová, Martina; Hobza, Roman; Široký, Jiří; Vyskot, Boris
2002 - English
Sex chromosomes of plant species Silene latifolia are the ideal model for evolutionary biology studies because they represent early stages of X and Y chromosomes history. We used fluorescence in situ hybridization (FISH) with bacterial artificial chromosome (BAC) clones in studies of chromosome structure to demonstrate that a majority of genes are located in subtelomeric regions of all chromosomes. Our results also indicate the absence of accumulation of main classes of repetitive sequences - retroelements and centromeric tandem repeats - in non-recombining parts of the sex chromosomes.
Keywords:
sex chromosomes; BAC-FISH; Silene latifolia
Available at various institutes of the ASCR
Structure and evolution of plant sex chromosomes: The use of BAC library
Sex chromosomes of plant species Silene latifolia are the ideal model for evolutionary biology studies because they represent early stages of X and Y chromosomes history. We used fluorescence in situ ...
Defects of pollen development in transgenic tobacco
Dubová, J.; Hájková, M.; Brzobohatý, Břetislav
2001 - English
Keywords:
pollen development; beta-glucosidase; transgenic plants
Available at various institutes of the ASCR
Defects of pollen development in transgenic tobacco
Identification of a cysteine residue conferring sensitivity of a maize beta-glucosidase to silver ion and alkylation treatment
Slaný, M.; Zouhar, J.; Brzobohatý, Břetislav
2001 - English
Keywords:
Cysteine residues; enzyme activity; beta-glucosidase
Available at various institutes of the ASCR
Identification of a cysteine residue conferring sensitivity of a maize beta-glucosidase to silver ion and alkylation treatment
Expression and single-step purification of the signal receiver domain of CKI1, a putative cytokinin receptor from Arabidopsis thaliana
Borkovcová, Petra; Zouhar, J.; Hejátko, Jan; Brzobohatý, Břetislav
2001 - English
Keywords:
CKI1 receiver domain; affinity chromatography; purification
Available at various institutes of the ASCR
Expression and single-step purification of the signal receiver domain of CKI1, a putative cytokinin receptor from Arabidopsis thaliana
Cytogenetics of Retinoblastoma
Bártová, Eva; Gajová, H.; Kozubek, Stanislav; Jirsová, Pavla; Kozubek, Michal
2001 - English
The study on the molecular cytogenetics of human retinoblastoma
Keywords:
retinoblastoma; cytogenetics
Available at various institutes of the ASCR
Cytogenetics of Retinoblastoma
The study on the molecular cytogenetics of human retinoblastoma
Topology and Structure of Dystrophin Gene on Active and Inactive Chromosome X in Human lymphocytes
Falk, Martin; Lukášová, Emilie; Kozubek, Stanislav; Kozubek, Michal
2001 - English
Study on the topology and structure of chromosome X and the dystrophin gene in human lymphocytes
Keywords:
chromosome X; human lymphocytes
Available at various institutes of the ASCR
Topology and Structure of Dystrophin Gene on Active and Inactive Chromosome X in Human lymphocytes
Study on the topology and structure of chromosome X and the dystrophin gene in human lymphocytes
Relationship between Chromosome Structure and Arrangement in the Interphase Nuclei and the Induction of Exchange Aberrations by Ionising Radiation
Cafourková, Alena; Lukášová, Emilie; Kozubek, Stanislav; Kozubek, Michal; Koutná, I.; Jirsová, Pavla; Bártová, Eva
2001 - English
Ionising radiation-induced chromosome aberations in relation to the structure of chromosomes and interphase nuclei
Keywords:
interphase nuclei; chromosome structure; exchange aberrations
Available at various institutes of the ASCR
Relationship between Chromosome Structure and Arrangement in the Interphase Nuclei and the Induction of Exchange Aberrations by Ionising Radiation
Ionising radiation-induced chromosome aberations in relation to the structure of chromosomes and interphase nuclei
Platinum Compounds. From DNA Modifications to Cancer Chemotherapy
Brabec, Viktor
2001 - English
The paper deals with DNA modifications and cancer chemotherapy based on platinum compounds
Keywords:
platinum; DNA; cancer chemotherapy
Available at various institutes of the ASCR
Platinum Compounds. From DNA Modifications to Cancer Chemotherapy
The paper deals with DNA modifications and cancer chemotherapy based on platinum compounds
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