Dark Web and Cryptocurrency
BOLATOV, Nugerbek
2023 - English This study aims to explore the principle of the interdependence and mutual influence of the Dark web and cryptocurrency. Specifically, it explores the terms "the Dark web" and "cryptocurrency", as well as distinguishes cryptocurrency from normal currency. Furthermore, it establishes a connection between the Dark web and cryptocurrency. In order to reach the aim of this study, multiple sources of literature had been analyzed, including books and online articles. The analysis of literature shows that there is a strong correlation between the existence of the Dark web and demand on cryptocurrency. Thus, the results of this paper conclude that the Dark web and cryptocurrency exist in symbiosis - cryptocurrency allows the Dark web to exist for its anonymity and impunity, while the Dark web guarantees demand on cryptocurrency. This study aims to explore the principle of the interdependence and mutual influence of the Dark web and cryptocurrency. Specifically, it explores the terms "the Dark web" and "cryptocurrency", as well as distinguishes cryptocurrency from normal currency. Furthermore, it establishes a connection between the Dark web and cryptocurrency. In order to reach the aim of this study, multiple sources of literature had been analyzed, including books and online articles. The analysis of literature shows that there is a strong correlation between the existence of the Dark web and demand on cryptocurrency. Thus, the results of this paper conclude that the Dark web and cryptocurrency exist in symbiosis - cryptocurrency allows the Dark web to exist for its anonymity and impunity, while the Dark web guarantees demand on cryptocurrency. Keywords: currency; cryptocurrency; the Internet; the Dark web; anonymity; illegal Available in the Digital Repository of University of South Bohemia. Dark Web and Cryptocurrency

This study aims to explore the principle of the interdependence and mutual influence of the Dark web and cryptocurrency. Specifically, it explores the terms "the Dark web" and "cryptocurrency", as ...

BOLATOV, Nugerbek
Jihočeská univerzita v Českých Budějovicích, 2023

Účinnost mykoparazitických a entomopatogenních hub proti houbovým původcům onemocnění rostlin
SINKALA, Natasha
2023 - English Entomopatogenní a mykoparazitické houby mají potenciál být využívány na místní i mezinárodní úrovni jako účinná a ekologicky šetrná strategie boje proti škůdcům a chorobám rostlin způsobeným houbovými patogeny. V bakalářské práci byla tes-tována účinnost užitečných hub v laboratorních podmínkách. Metodika zahrnovala izolaci a popis užitečných druhů hub a následné testování užitečných hub s poten-cionálními mykoparazitickými a antagonistickými účinky. Účinnost hub byla tes-tována na patogenech, které byly převážně odizolovány z osiva nebo nemocných ros-tlin luskovin. V rámci pokusů bylo zjištěno, že všechny kmeny Trichoderma izolo-vané z půd ČR prokázaly mykoparazitický efekt na vybrané patogeny. Nicméně, účinnost kmenů závisela na druhu houbového patogenu. Hodnocena byla i produkce spor kmenů Trichoderma jak v kontrolní variantě, tak i ve vzájemné kombinaci s patogeny. Výsledky opět prokázaly rozdíly v produkci spor u jednotlivých kmenů Trichoderma. Zároveň byl hodnocen mykoparazitický účinek entomopatogenních hub Isaria fumosorosea a Akanthomyces attenuatus proti patogenu Botrytis cinerea. Výsledky ukázaly, že ačkoli I. fumosorosea a A. attenuatus vykazovaly významný mykoparazitický účinek proti Botrytis cinerea, nejvyšší účinnost proti patogenu prokázala mykoparazitická houba T.virens. Zjištění naznačila, že jak mykoparazitické, tak entomopatogenní houby jsou udržitelnou alternativou umělých pesticidů v boji proti houbovým původcům chorob rostlin. Entomopathogenic and mycoparasitic fungi have the potential to be used both locally and internationally as an effective and environmentally friendly strategy for the man-agement of pests and plant diseases caused by fungal pathogens. In this bachelor thesis, the efficacy of beneficial fungi was tested under laboratory conditions. The methodology involved isolation and description of useful fungal species and subse-quent testing of useful fungi with potential mycoparasitic and antagonistic effects. The efficacy of the fungi was tested on pathogens that were mainly isolated from seeds or diseased legume plants. The experiments revealed that all Trichoderma strains isolated from soil of the Czech Republic showed mycoparasitic effect on se-lected pathogens. However, the efficacy of the strains depended on the type of fun-gal pathogen. Spore production of Trichoderma strains was also evaluated both in the control variant as well as the mutual interactions with pathogens. The results again showed differences in spore production among the Trichoderma strains. The myco-parasitic effect of the entomopathogenic fungi Isaria fumosorosea and Akanthomyces attenuatus against the pathogen Botrytis cinerea was also evaluated. The results re-vealed that although I. fumosorosea and A. attenuatus showed a significant mycopar-asitic effect against Botrytis cinerea, the mycoparasitic fungus T. virens showed the highest efficacy against the pathogen. The findings indicated that both mycoparasitic and entomopathogenic fungi are sustainable alternatives to artificial pesticides in the fight against fungal agents of plant diseases. Keywords: biologicka ochrana; mykoparazitické houby; entomopatogenní houby; Trichoderma spp.; Akanthomyces attenuatus; Isaria fumosorosea; patogeny rostlin Available in the Digital Repository of University of South Bohemia. Účinnost mykoparazitických a entomopatogenních hub proti houbovým původcům onemocnění rostlin

Entomopatogenní a mykoparazitické houby mají potenciál být využívány na místní i mezinárodní úrovni jako účinná a ekologicky šetrná strategie boje proti škůdcům a chorobám rostlin způsobeným houbovými ...

SINKALA, Natasha
Jihočeská univerzita v Českých Budějovicích, 2023

Arthur Ransome, (nejen) autor dětských knih o Vlaštovkách a Amazonkách
ZMYKALO, Daniel
2023 - English Předmětem zkoumání bakalářské práce je život a dílo britského autora dětských knih příběhů o Vlaštovkách a Amazonkách Arthura Ransoma. Práce v úvodu představí životopis kontroverzního autora, který proslul jako autor série dětských příběhů a zároveň naplňoval představu bohémského životního stylu a nevšedních zájmů v kulturní i politické oblasti. Cílem práce je kriticky zhodnotit rozporuplné osudy a peripetie autorova života na základě biografických a autobiografických pramenů (Brogan, Chambers, Hyland, Ransome) a pokusit se vystihnout podstatu dětských dobrodružných příběhů meziválečného období v Anglii se zaměřením na charakteristiku regionu tzv. Jezerní oblasti (Lake District) a jeho kulturního dědictví. V závěru práce porovná kritickou odezvu a kulturní význam Ransomových příběhů ve 20. století a v současnosti. The subject of the bachelor's thesis will be the life and literary work of the English author Arthur Ransome. The work will first focus on a detailed description of the author's life and what led him to write a children's book series, even though he lived a bohemian life and had extraordinary interests in culture and politics. The aim of the thesis is to critically evaluate the contradictory destinies and vicissitudes of the author's life based on biographical and autobiographical sources (Brogan, Chambers, Hyland, Ransome) and try to capture the essence of children's adventure stories in England with a focus on the characteristics of the Lake District) and its cultural heritage. At the end of the work, it compares the critical response and cultural significance of Ransom's stories in the 20th century and the present. Keywords: Vlaštovky a Amazonky; Jezerní oblast; Arthur Ransome; Dětská literatura Available in the Digital Repository of University of South Bohemia. Arthur Ransome, (nejen) autor dětských knih o Vlaštovkách a Amazonkách

Předmětem zkoumání bakalářské práce je život a dílo britského autora dětských knih příběhů o Vlaštovkách a Amazonkách Arthura Ransoma. Práce v úvodu představí životopis kontroverzního autora, který ...

ZMYKALO, Daniel
Jihočeská univerzita v Českých Budějovicích, 2023

Expression and purification of Tick-borne encephalitis virus Capsid protein
SCHMELZER, Jakob
2022 - English Tick-borne encephalitis virus (TBEV) is an important pathogen of the family Flaviviridae with an emerging health concern. Virus causes severe neurological disorders to human population in Europa and Asia. Despite effective vaccine, there is no specific antiviral treatment till now. The capsid protein encapsules the viral positive-sense single-stranded RNA and is essential in packaging and assembling of new virions within the host. A deeper understanding of these interactions and their implications should lead to new antiviral strategies and drug design. This thesis deals with the expression and purification of the capsid protein without membrane anchor using Escherichia coli expression system. Tick-borne encephalitis virus (TBEV) is an important pathogen of the family Flaviviridae with an emerging health concern. Virus causes severe neurological disorders to human population in Europa and Asia. Despite effective vaccine, there is no specific antiviral treatment till now. The capsid protein encapsules the viral positive-sense single-stranded RNA and is essential in packaging and assembling of new virions within the host. A deeper understanding of these interactions and their implications should lead to new antiviral strategies and drug design. This thesis deals with the expression and purification of the capsid protein without membrane anchor using Escherichia coli expression system. Keywords: Flavivirus; Tick-borne encephalitis virus; Capsid protein; Cloning; Expression; IPTG; BL21-CodonPlus (DE3)-RIPL; Immobilized Metal Affinity Chromatography Available in the Digital Repository of University of South Bohemia. Expression and purification of Tick-borne encephalitis virus Capsid protein

Tick-borne encephalitis virus (TBEV) is an important pathogen of the family Flaviviridae with an emerging health concern. Virus causes severe neurological disorders to human population in Europa and ...

SCHMELZER, Jakob
Jihočeská univerzita v Českých Budějovicích, 2022

THE SECRETS ENCODED IN THE DRAFT GENOME OF Lentzea sp. STRAIN BCCO 10_0061 ISOLATED FROM RECLAIMED MINE HEAPS
KELLER, Moritz
2022 - English Many Actinobacteria including Lentzea have been found to produce medically viable compounds like antibiotics and antitumor agents. This thesis deals with genome sequencing of Lentzea sp. BCCO 10_0061, assembly, annotation and evaluation of the potential for secondary metabolite production, as well as phylogenetic classification and creation of a metabolic profile. Many Actinobacteria including Lentzea have been found to produce medically viable compounds like antibiotics and antitumor agents. This thesis deals with genome sequencing of Lentzea sp. BCCO 10_0061, assembly, annotation and evaluation of the potential for secondary metabolite production, as well as phylogenetic classification and creation of a metabolic profile. Keywords: Actinobacteria; Lentzea; genome assembly; iso-migrastatin; nystatin A1; biosynthetic gene clusters Available in the Digital Repository of University of South Bohemia. THE SECRETS ENCODED IN THE DRAFT GENOME OF Lentzea sp. STRAIN BCCO 10_0061 ISOLATED FROM RECLAIMED MINE HEAPS

Many Actinobacteria including Lentzea have been found to produce medically viable compounds like antibiotics and antitumor agents. This thesis deals with genome sequencing of Lentzea sp. BCCO ...

KELLER, Moritz
Jihočeská univerzita v Českých Budějovicích, 2022

Calling Haplotypes from duplex sequencing data and its application: a case study with Erbb2 data
MOUKBEL ALI ALDAWLA, Shehab
2022 - English Available in the Digital Repository of University of South Bohemia. Calling Haplotypes from duplex sequencing data and its application: a case study with Erbb2 data

MOUKBEL ALI ALDAWLA, Shehab
Jihočeská univerzita v Českých Budějovicích, 2022

Investment Market Tool for Young People
TORRES GARCIA, Miguel Ernesto
2022 - English This thesis presents an investment decision tool, allowing to select the best strategy for maximizing their resources, when young people in the Czech Republic are faced with the dilemma of buying a house or living via rent and making an investment with a possible extra money they have. Nowadays, the housing market might be too expensive for the common people. Based on national average dwelling prices and average income, saving for the down payment might take several years. Also, the mortgage monthly payments can take a sizable part of a person income. The tool is written in Microsoft Excel? using VBA programming language to predict investments portfolios return and compare it to a hypothetical mortgage. Several scenarios can be saved for further analysis and the tool has the possibility to include further investment options and prediction techniques. Several examples are given to show the utility as well as a survey of potential users that have evaluated this tool. This thesis presents an investment decision tool, allowing to select the best strategy for maximizing their resources, when young people in the Czech Republic are faced with the dilemma of buying a house or living via rent and making an investment with a possible extra money they have. Nowadays, the housing market might be too expensive for the common people. Based on national average dwelling prices and average income, saving for the down payment might take several years. Also, the mortgage monthly payments can take a sizable part of a person income. The tool is written in Microsoft Excel? using VBA programming language to predict investments portfolios return and compare it to a hypothetical mortgage. Several scenarios can be saved for further analysis and the tool has the possibility to include further investment options and prediction techniques. Several examples are given to show the utility as well as a survey of potential users that have evaluated this tool. Keywords: Mortgage; Investment; Excel; Forecast Available in the Digital Repository of University of South Bohemia. Investment Market Tool for Young People

This thesis presents an investment decision tool, allowing to select the best strategy for maximizing their resources, when young people in the Czech Republic are faced with the dilemma of buying a ...

TORRES GARCIA, Miguel Ernesto
Jihočeská univerzita v Českých Budějovicích, 2022

Cathepsin L of Sphaerospora molnari - localisation, function and diagnostic tools
NECHVILE, Rudolf Lukas
2022 - English The aim of this thesis was to develop diagnostic and quantitative assays/tools based on western blotting, confocal microscopy and immunogold electron microscopy, as well as flow cytometry, to determine the localisation and potential function of the highly expressed cathepsin L of the myxozoan parasite Sphaerospora molnari (SmolCL) in its fish host, the common carp. Furthermore, a recombinant SmolCL was used in a vaccine trial to estimate its potential for raising antibodies in carp and test their immunoprotective potential towards S. molnari. This thesis provides new methodological tools for research and allows a greater understanding of myxozoan parasite-fish host interactions based on proteolytic enzymes. The aim of this thesis was to develop diagnostic and quantitative assays/tools based on western blotting, confocal microscopy and immunogold electron microscopy, as well as flow cytometry, to determine the localisation and potential function of the highly expressed cathepsin L of the myxozoan parasite Sphaerospora molnari (SmolCL) in its fish host, the common carp. Furthermore, a recombinant SmolCL was used in a vaccine trial to estimate its potential for raising antibodies in carp and test their immunoprotective potential towards S. molnari. This thesis provides new methodological tools for research and allows a greater understanding of myxozoan parasite-fish host interactions based on proteolytic enzymes. Keywords: Sphaerospora molnari; Myxozoa; cathepsin L; carp; cnidarian parasite; cysteine protease; recombinant protein; polyclonal antibody; recombinant protease vaccine; western blot; flow cytometry; immunofluorescence microscopy; immunogold TEM; qPCR; ELISA Available in the Digital Repository of University of South Bohemia. Cathepsin L of Sphaerospora molnari - localisation, function and diagnostic tools

The aim of this thesis was to develop diagnostic and quantitative assays/tools based on western blotting, confocal microscopy and immunogold electron microscopy, as well as flow cytometry, to ...

NECHVILE, Rudolf Lukas
Jihočeská univerzita v Českých Budějovicích, 2022

Dissemination of Cordyceps fumosorosea by entomopathogenic Nematodes
HOPFGARTNER, Miriam
2022 - English C. fumosorosea is an entomopathogenic fungus that has been used as a biocontrol agent against various insect hosts for some time. Its efficacy as an entomopathogen can be increased by joined application of the fungus with entomopathogenic nematodes. The aims of this thesis are to investigate the impact of the foraging strategy of different nematode species on the spore dispersal of C. fumosorosea and the significance the nematode's 2nd stage cuticle holds for spore dissemination. Additionally, novel approaches for the enhancement of spore transmission in soil environments via adhesives are explored. C. fumosorosea is an entomopathogenic fungus that has been used as a biocontrol agent against various insect hosts for some time. Its efficacy as an entomopathogen can be increased by joined application of the fungus with entomopathogenic nematodes. The aims of this thesis are to investigate the impact of the foraging strategy of different nematode species on the spore dispersal of C. fumosorosea and the significance the nematode's 2nd stage cuticle holds for spore dissemination. Additionally, novel approaches for the enhancement of spore transmission in soil environments via adhesives are explored. Keywords: entomopathogenic nematodes; steinernema feltiae; steinernema carpocapsae; heterorhabditis bacteriophora; entomopathogenic fungi; cordyceps fumosorosea; conidia; blastospores; spore dispersal; adhesives; PDA plates; soil substrates; galleria mellonella Available in the Digital Repository of University of South Bohemia. Dissemination of Cordyceps fumosorosea by entomopathogenic Nematodes

C. fumosorosea is an entomopathogenic fungus that has been used as a biocontrol agent against various insect hosts for some time. Its efficacy as an entomopathogen can be increased by joined ...

HOPFGARTNER, Miriam
Jihočeská univerzita v Českých Budějovicích, 2022

Representation of Masculinity in J.R.R. Tolkien's The Lord of the Rings
BLÁHOVCOVÁ, Václava
2022 - English Tato bakalářská práce se zabývá rozličnými typy maskulinity zobrazenými v mužských postavách v díle Pán prstenů J.R.R. Tolkiena a jejich porovnáním se stereotypy genderových rolí a zobrazením mužských postav ve fantasy žánru. První část se soustředí na Tolkienův život, vlivy na jeho dílo a přehled kritiky Tolkienova díla. Také představuje debatu o sexismu spojeného se stereotypním zobrazením ženských postav a jejich nedostatek v příběhu. Druhá část obsahuje stručný úvod do genderových studií feminity a maskulinity a žánru fantasy. Ve třetí části je analýza hlavních ras Středozemě a vybraných postav - Aragorna, Samvěda Křepelky, Froda Pytlíka a bratrů Boromira a Faramira - v kontextu maskulinity. This bachelor thesis deals with various types of masculinity as represented by male characters in J.R.R. Tolkien's The Lord of the Rings and their comparison with stereotypical gender roles and the portrayal of male characters in the fantasy literature genre. The first part focuses on Tolkien's life and the influences on his writing and gives a review of the criticism of Tolkien's work. It also introduces the debate of sexism related to the stereotypical portrayal of female characters and their scarcity in the story. The second part is a brief introduction to the gender studies of femininity and masculinity, and the fantasy genre. The third part is the analysis of the main races of Middle-earth and selected characters - Aragorn, Samwise Gamgee, Frodo Baggins, and fraternal pair Boromir and Faramir - in the context of masculinity. Keywords: J.R.R. Tolkien; The Lord of the Rings; Pán prstenů; maskulinita; feminita; fantasy žánr; fantasy postavy; stereotypy; reprezentace Available in the Digital Repository of University of South Bohemia. Representation of Masculinity in J.R.R. Tolkien's The Lord of the Rings

Tato bakalářská práce se zabývá rozličnými typy maskulinity zobrazenými v mužských postavách v díle Pán prstenů J.R.R. Tolkiena a jejich porovnáním se stereotypy genderových rolí a zobrazením mužských ...

BLÁHOVCOVÁ, Václava
Jihočeská univerzita v Českých Budějovicích, 2022