Number of found documents: 545
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Vinyl-modified DNA and its cleavage by restriction endonucleases
Mačková, Michaela; Hocek, Michal
2014 - English
Three types of vinyl modified nucleoside triphosphates (dNVTPs) were prepared by single step aqueous Suzuki- Miyaura cross-coupling reaction of halogenated 2’-deoxynucleotides with vinyltrifluoroborate. These dNVTPs were enzymatically incorporated into DNA in Primer extension experiments and Polymerase chain reaction. Primer extension products were then tested for cleavage by series of type II restriction enzymes and the results were compared with a cleavage of ethynyl-modified DNA. Keywords: dNVTPs; DNA; cross-coupling reaction Available at various institutes of the ASCR
Vinyl-modified DNA and its cleavage by restriction endonucleases

Three types of vinyl modified nucleoside triphosphates (dNVTPs) were prepared by single step aqueous Suzuki- Miyaura cross-coupling reaction of halogenated 2’-deoxynucleotides with ...

Mačková, Michaela; Hocek, Michal
Ústav organické chemie a biochemie, 2014

Enzymatic decomposition of a locked nucleoside phosphoramidate monitored by 31P NMR spectroscopy
Procházková, Eliška; Hřebabecký, Hubert; Nencka, Radim; Dračínský, Martin
2014 - English
Phosphoramidate derivatives are often used as prodrugs of modified nucleosides. The crucial step for the biologically active compound release in cells is then enzymatic hydrolysis of the phosphoramidate derivative. The enzymatic decomposition of a nucleoside prodrug 1 was monitored using 31P NMR spectroscopy and the structure of the reaction products was determined by 1D and 2D (COSY, HSQC, HMBC) NMR experiments and compared with the products obtained with non-enzymatic hydrolysis of the prodrug. Keywords: phosphoramidate derivatives; enzymatic hydrolysis; NMR spectroscopy Available at various institutes of the ASCR
Enzymatic decomposition of a locked nucleoside phosphoramidate monitored by 31P NMR spectroscopy

Phosphoramidate derivatives are often used as prodrugs of modified nucleosides. The crucial step for the biologically active compound release in cells is then enzymatic hydrolysis of the ...

Procházková, Eliška; Hřebabecký, Hubert; Nencka, Radim; Dračínský, Martin
Ústav organické chemie a biochemie, 2014

Novel conformationally locked nucleosides and nucleotides based on bicyclo[3.2.1]octane scaffold as a pseudosugar moiety
Šála, Michal; Dejmek, Milan; Procházková, Eliška; Hřebabecký, Hubert; Rybáček, Jiří; Dračínský, Martin; Novák, Pavel; Rosenbergová, Šárka; Fukal, J.; Sychrovský, Vladimír; Rosenberg, Ivan; Nencka, Radim
2014 - English
A route to a series of novel carbocyclic nucleosides locked in North conformation with bicyclo[3.2.1]octane scaffold was developed. Prepared nucleosides served as a starting material for the synthesis of modified oligomers [d(GCATATCAC), r(GCAUAUCAC), and A9]. Biological effects of the prepared nucleosides as well as the hybridization properties of the appropriate duplexes were evaluated. Keywords: locked nucleosides; LNA Available at various institutes of the ASCR
Novel conformationally locked nucleosides and nucleotides based on bicyclo[3.2.1]octane scaffold as a pseudosugar moiety

A route to a series of novel carbocyclic nucleosides locked in North conformation with bicyclo[3.2.1]octane scaffold was developed. Prepared nucleosides served as a starting material for the ...

Šála, Michal; Dejmek, Milan; Procházková, Eliška; Hřebabecký, Hubert; Rybáček, Jiří; Dračínský, Martin; Novák, Pavel; Rosenbergová, Šárka; Fukal, J.; Sychrovský, Vladimír; Rosenberg, Ivan; Nencka, Radim
Ústav organické chemie a biochemie, 2014

Bi-substrate inhobitors of human pyrimidine 5’-nucleosidases
Šimák, Ondřej; Pachl, Petr; Jandušík, T.; Brynda, Jiří; Buděšínský, Miloš; Rosenberg, Ivan
2014 - English
The work is aimed at the synthesis of bi-substrate inhibitors of 5’-nucleosidases. The pyrimidine nucleobase of lead compound was derivatized in the 5-position with various phosphonic acids differing in conformational flexibility and in the length of alkyl side chain carrying phosphonate group. The phosphonic acid with optimal linker can reach the phosphate binding side and make strong interaction with the enzyme. Inhibition activity of such inhibitors and crystal structure of inhibitor-enzyme complex will be presented. Keywords: 5’-nucleosidases; NPAs; bi-substrate inhibitors Available at various institutes of the ASCR
Bi-substrate inhobitors of human pyrimidine 5’-nucleosidases

The work is aimed at the synthesis of bi-substrate inhibitors of 5’-nucleosidases. The pyrimidine nucleobase of lead compound was derivatized in the 5-position with various phosphonic acids differing ...

Šimák, Ondřej; Pachl, Petr; Jandušík, T.; Brynda, Jiří; Buděšínský, Miloš; Rosenberg, Ivan
Ústav organické chemie a biochemie, 2014

Synthesis and biological activity of thiophene fused 7-deazapurine ribonucleosides
Tichý, Michal; Hocek, Michal
2014 - English
A series of thieno[2’,3’:4,5]pyrrolo[2,3-d]pyrimidine ribonucleosides bearing various alkyl, amino or hetaryl groups in position 4 has been prepared and all compounds have been tested for cytostatic and antiviral activities (HCV and Dengue viruses). Keywords: nucleosides; cytostatics; 7-deazapurine ribonucleosides Available at various institutes of the ASCR
Synthesis and biological activity of thiophene fused 7-deazapurine ribonucleosides

A series of thieno[2’,3’:4,5]pyrrolo[2,3-d]pyrimidine ribonucleosides bearing various alkyl, amino or hetaryl groups in position 4 has been prepared and all compounds have been tested for cytostatic ...

Tichý, Michal; Hocek, Michal
Ústav organické chemie a biochemie, 2014

Ambientní ionizační techniky
Vrkoslav, Vladimír
2014 - Czech
Byly popsány principy nejdůležitějších ambientních ionizačních technik, jejich rozdělení a příklady využití. Classification, principles and application examples of the most important ambient ionization techniques were described. Keywords: mass spectrometry; analysis; desorption Available at various institutes of the ASCR
Ambientní ionizační techniky

Byly popsány principy nejdůležitějších ambientních ionizačních technik, jejich rozdělení a příklady využití....

Vrkoslav, Vladimír
Ústav organické chemie a biochemie, 2014

(MA)LDI MS a MSI malých molekul
Vrkoslav, Vladimír
2014 - Czech
Byla vysvětlena specifika použití MALDI/MS a LDI/MS pro analýzu malých molekul. Using of MALDI/MS and LDI/MS for analysis of small molecular was explained. Keywords: mass spectrometry; mass spectrometry imaging; matrix assisted laser desorption/ ionization Available at various institutes of the ASCR
(MA)LDI MS a MSI malých molekul

Byla vysvětlena specifika použití MALDI/MS a LDI/MS pro analýzu malých molekul.


Using of MALDI/MS and LDI/MS for analysis of small molecular was explained.

Vrkoslav, Vladimír
Ústav organické chemie a biochemie, 2014

Analysis and characterization of antimicrobial peptides by capillary electrophoresis
Tůmová, Tereza; Monincová, Lenka; Čeřovský, Václav; Kašička, Václav
2014 - English
Capillary electrophoresis has been employed for quantitative and qualitative analysis, separation and characterization of antimicrobial peptides – halictines. In addition to purity degree of synthetic peptides, also their effective and ionic mobilities and charges within a wide pH range were determined. Keywords: capillary electrophoresis; halictines; physico-chemical parameters Available at various institutes of the ASCR
Analysis and characterization of antimicrobial peptides by capillary electrophoresis

Capillary electrophoresis has been employed for quantitative and qualitative analysis, separation and characterization of antimicrobial peptides – halictines. In addition to purity degree of synthetic ...

Tůmová, Tereza; Monincová, Lenka; Čeřovský, Václav; Kašička, Václav
Ústav organické chemie a biochemie, 2014

Development and applications of ionization techniques in ambient mass spectrometry
Rejšek, Jan; Vrkoslav, Vladimír; Cvačka, Josef
2014 - English
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization techniques in the ambient mass spectometry. Herein, they were investigated in two research areas: (1) TLC analysis. (2) Localization of insect defense glands. Keywords: ambient mass spectrometry; desorption electrospray ionization; desorption atmospheric pressure photoionization; thin-layer chromatography; insect defense compounds; mass spectrometry imaging Available at various institutes of the ASCR
Development and applications of ionization techniques in ambient mass spectrometry

Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization ...

Rejšek, Jan; Vrkoslav, Vladimír; Cvačka, Josef
Ústav organické chemie a biochemie, 2014

Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode
Ferenčíková, Z.; Daňhel, Aleš; Riedl, Jan; Hocek, Michal; Fojta, Miroslav
2014 - English
4-aminophthalimide (API), covalently attached to nucleotide (dCTP or dATP) has recently beendesigned as a fluorescent label for the detection of DNA-protein interaction. However API is electrochemically reducible and therefore it is possible to use alternatively the API-modified nuclotides as DNA redox labels. The electrochemical behavior of labeled nucleotides and labeled DNA was studied using cyclic voltammetry and transfer stripping cyclic voltammetry at hanging mercury drop electrode. Observed CVs showed irreversible reduction signals of 4-aminophthalimide label without production of consequently oxidizable/reducible species. Keywords: labeled nucleotide; 4-aminophthalimide; primer extension; DNA; hanging mercury drop electrode; voltammetry Available at various institutes of the ASCR
Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode

4-aminophthalimide (API), covalently attached to nucleotide (dCTP or dATP) has recently beendesigned as a fluorescent label for the detection of DNA-protein interaction. However API is ...

Ferenčíková, Z.; Daňhel, Aleš; Riedl, Jan; Hocek, Michal; Fojta, Miroslav
Ústav organické chemie a biochemie, 2014

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