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Capillary isotachophoresis with ESI-MS detection: Ultrahighly sensitve analysis of diclofenac and ibuprofen in waters
Malá, Zdeňka; Gebauer, Petr; Boček, Petr
2015 - English
This work shows that CE-MS can reach same sensitivity as HPLC-MS, when it is peformed in the ITP-MS mode. By combination of ITP-ESI-MS with an offline SPE preconcentration step and optimization of MS signal collection, LODs below 2x10-12 M can be reached, corresponding to of 0.6 ng/L of diclofenac (Dic) and 0.4 ng/L of ibuprofen (Ibu), compatible to that of SPE-HPLC-MS.
Keywords:
CE-MS; HPLC-MS; ITP-MS; ITP-ESI-MS
Fulltext is available at external website.
Capillary isotachophoresis with ESI-MS detection: Ultrahighly sensitve analysis of diclofenac and ibuprofen in waters
This work shows that CE-MS can reach same sensitivity as HPLC-MS, when it is peformed in the ITP-MS mode. By combination of ITP-ESI-MS with an offline SPE preconcentration step and optimization of MS ...
Novel microextraction techniques in pretreatment of complex samples
Kubáň, Pavel; Pantůčková, Pavla; Šlampová, Andrea; Boček, Petr
2015 - English
Fundamental considerations and practical applications of various microextraction techniques for pretreatment of samples with complex matrices are summarized in this lecture. Special emphasis is devoted to electrically driven microextractions, such as electromembrane extraction across supported liquid membranes, their down-scaling to micro- and nano- format and their applications in capillary electrophoretic analyses of biological samples.
Keywords:
electromembrane extraction; supported liquid membranes; free liquid membranes; capillary electrophoresis
Fulltext is available at external website.
Novel microextraction techniques in pretreatment of complex samples
Fundamental considerations and practical applications of various microextraction techniques for pretreatment of samples with complex matrices are summarized in this lecture. Special emphasis is ...
Subcritical extraction as sample treatment method for antioxidant screening of various plant extracts
Hohnová, Barbora; Šťavíková, Lenka; Karásek, Pavel; Roth, Michal
2014 - English
Pressurized Hot Water Extraction performed in both static and dynamic modes followed by HPLC with UV-ViS detection was employed for fast and efficient determination of rutin and quercetin from various berries. Moreover, the antioxidant power of subcritical extracts applying •DPPH was evaluated.
Keywords:
PLE; HPLC; DPPH
Fulltext is available at external website.
Subcritical extraction as sample treatment method for antioxidant screening of various plant extracts
Pressurized Hot Water Extraction performed in both static and dynamic modes followed by HPLC with UV-ViS detection was employed for fast and efficient determination of rutin and quercetin from various ...
Extraction of bioavailable proportion of metals in diesel emissions and atmospheric aerosols using artificial lung fluids
Coufalík, Pavel; Mikuška, Pavel; Křůmal, Kamil; Večeřa, Zbyněk; Vojtíšek, M.; Matoušek, Tomáš
2014 - English
The determination of bioavailable proportion of selected metals in biofuel exhaust and atmospheric aerosols, and a comparison of artificial lung fluids in relation to the yield of extraction were performed.
Keywords:
extraction; metal; aerosol
Fulltext is available at external website.
Extraction of bioavailable proportion of metals in diesel emissions and atmospheric aerosols using artificial lung fluids
The determination of bioavailable proportion of selected metals in biofuel exhaust and atmospheric aerosols, and a comparison of artificial lung fluids in relation to the yield of extraction were ...
Sample preparation for single cell analysis
Adamová, Eva; Basova, E. Y.; Potáčová, Anna; Foret, František; Matalová, Eva; Klepárník, Karel
2014 - English
Development of sensitive, miniaturized and fast methods aims to reliable analyze even small amounts of samples. Micromanipulation systems such as laser capture microdissection enable to collect just single cells from cell suspensions or tissue samples. One option for further analysis is the bioluminescence reaction based on luciferin/luciferase conversion. This approach was used for detection of active caspases, caspase-3/7. Droplets based microfluidic systems provide benefits in biological, pharmaceutical and chemical research.
Keywords:
single cell analysis; micromanipulation; bioluminiscence
Fulltext is available at external website.
Sample preparation for single cell analysis
Development of sensitive, miniaturized and fast methods aims to reliable analyze even small amounts of samples. Micromanipulation systems such as laser capture microdissection enable to collect just ...
Theoretical principles of capillary isotachophoresis in moving boundary systems
Gebauer, Petr; Malá, Zdeňka; Boček, Petr
2014 - English
This contribution provides fundamental theoretical background of ITP in moving-boundary systems. It is based on the approximation of capillary ITP as a 1D process and the general condition of ITP migration, i.e., the existence of a self-sharpening boundary capable to form a stack of zones moving with equal velocities.
Keywords:
capillary isotachophoresis; moving boundary system; stacking capability
Fulltext is available at external website.
Theoretical principles of capillary isotachophoresis in moving boundary systems
This contribution provides fundamental theoretical background of ITP in moving-boundary systems. It is based on the approximation of capillary ITP as a 1D process and the general condition of ITP ...
Separation of tryptic digest of cytochrome c within a long nanoelectrospray tip
Týčová, Anna; Foret, František
2014 - English
Capillary electrophoresis coupled to mass spectrometry (CE-MS) posses powerful tool for separation and detection of wide range of ionic species. To avoid complicated constructions of interfaces we have conducted the separation within a long and thin nanoelectrospray tip [1]. We have investigated main properties of such an experimental design on a complex sample of tryptic digest of cytochrome c.
Keywords:
capillary electrophoresis; cytochrome c; nanoelectrospray
Fulltext is available at external website.
Separation of tryptic digest of cytochrome c within a long nanoelectrospray tip
Capillary electrophoresis coupled to mass spectrometry (CE-MS) posses powerful tool for separation and detection of wide range of ionic species. To avoid complicated constructions of interfaces we ...
Arsenic speciation analysis by HPLC postcolumn hydride generation and detection by atomic fluorescence spectrometry
Marschner, Karel; Musil, Stanislav; Rychlovský, P.; Dědina, Jiří
2014 - English
The aim of this contribution is to present a new method of hydride generation that enables to generate arsines from iAs , iAs , MMA and DMA in a flow injection mode with the same efficiency and in the next step connection of this hydride generator with HPLC column.
Keywords:
arsenic speciation analysis; hydride generation; atomic fluorescence spectrometry
Available in digital repository of the ASCR
Arsenic speciation analysis by HPLC postcolumn hydride generation and detection by atomic fluorescence spectrometry
The aim of this contribution is to present a new method of hydride generation that enables to generate arsines from iAs , iAs , MMA and DMA in a flow injection mode with the same efficiency and in the ...
Analysis of individual organelles
Václavek, Tomáš; Manion-Fisher, D.; Muratore, K.; Taylor, T.; Křenková, Jana; Foret, František; Arriaga, E. A.
2014 - English
This poster describes a method of organelle analysis using capillary electrophoresis instrumentation with high sensitivity laser induced fluorescence detection.
Keywords:
organelle; capillary; fluorescence
Fulltext is available at external website.
Analysis of individual organelles
This poster describes a method of organelle analysis using capillary electrophoresis instrumentation with high sensitivity laser induced fluorescence detection.
Identification of nosocomial infection by electrophoretic techniques
Kubesová, Anna; Moravcová, Dana; Tesařová, Marie; Horká, Marie
2014 - English
The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic procedures to modern molecular biology based methods. However, conventional laboratory methods are time consuming, laborious, and they may provide both false positive or negative results, especially for closely related microorganisms. In this study, we suggest capillary electrophoresis techniques for differentiation and characterization of Methicillin-resistant and Methicillin-susceptible Staphylococcus aureus.
Keywords:
capillary electrophoresis; nosocomial infection; Staphylococcus aureus
Fulltext is available at external website.
Identification of nosocomial infection by electrophoretic techniques
The detection and identification of pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic procedures to modern molecular biology based ...
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