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164
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CE-nESI/MS in electrode-free design: narrowing the separation channel to 5 μm
Týčová, Anna; Foret, František
2016 - English
Capillary electrophoresis-nanospray/mass spectrometry (CE-nESI/MS) in electrodefree\narrangement represents a very simple instrumentation for analysis of biomolecules.\nSo far, CE-nESI/MS analyses in this design were limited to 10-75 μm ID of the\nseparation channel. Although the work with narrower dimensions brings several\nchallenges, there is a great potential for better sensitivity, improved separation power\nand lower sample consumption. This work is devoted to some practical aspects of\nexperiments in narrow bore channels and systematic evaluation of CE-nESI/MS\nanalyses conducted in capillaries of 25, 15 and 5 μm ID.
Keywords:
CE-nESI/MS; electrode-free arragement
Available in digital repository of the ASCR
CE-nESI/MS in electrode-free design: narrowing the separation channel to 5 μm
Capillary electrophoresis-nanospray/mass spectrometry (CE-nESI/MS) in electrodefree\narrangement represents a very simple instrumentation for analysis of biomolecules.\nSo far, CE-nESI/MS analyses in ...
Comparative evaluation of protein composition in human breast cancer cells using mass spectrometry
Flodrová, Dana; Toporová, L.; Macejová, D.; Laštovičková, Markéta; Brtko, J.; Bobálová, Janette
2016 - English
Bottom-up proteomic approach was used for detailed characterization of proteins from\ntwo human tumour cell lines representing major clinically different types of breast\ncancer. The aim was to show the differences between them on proteomic level. Here\nwe present almost 100 unequivocally identified proteins out of which 60 were mutually\ndifferently expressed for MDA-MB-231 and MCF-7. Some well-known breast cancer\nmarkers like annexins A1, A2 and vimentin were found in the MDA-MB-231 cell line.\nOn the other hand, MCF-7 cells were found to be positive for cytokeratins and keratins\nand thus we were able to distinguish both cell lines sufficiently.
Keywords:
mass spectrometry; breast cancer; human tumour; bottom-up proteomic approach
Available in digital repository of the ASCR
Comparative evaluation of protein composition in human breast cancer cells using mass spectrometry
Bottom-up proteomic approach was used for detailed characterization of proteins from\ntwo human tumour cell lines representing major clinically different types of breast\ncancer. The aim was to show ...
Hexahistidine labeling of oligosaccharides
Partyka, Jan; Křenková, Jana; Foret, František
2016 - English
We have presented another approach for labeling of oligosaccharides suitable for\ncapillary zone electrophoresis coupled with mass spectrometric detection.\nOligosaccharides labeled by peptides with hexahistidine sequence have faster\nseparation resulting in two or three times shorter time in comparison to oligosaccharides\nwith singly charged labels.
Keywords:
capillary zone electrophoresis; mass spectrometric detection; oligosaccharides
Available in digital repository of the ASCR
Hexahistidine labeling of oligosaccharides
We have presented another approach for labeling of oligosaccharides suitable for\ncapillary zone electrophoresis coupled with mass spectrometric detection.\nOligosaccharides labeled by peptides with ...
Organic compounds in PM1 aerosol in the Central Bohemian Region in the Czech Republic
Křůmal, Kamil; Mikuška, Pavel; Coufalík, Pavel; Večeřa, Zbyněk
2015 - English
Contribution summarizes the concentrations of monosaccharide anhydrides, resin acids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes, hopanes, steranes and polyaromatic hydrocarbons in PM1 aerosols in small town Celakovice in winter. According to concentrations of organic markers, the identification of emission sources in Celakovice is discussed.
Keywords:
organic tracer; urban aerosol; PM1
Fulltext is available at external website.
Organic compounds in PM1 aerosol in the Central Bohemian Region in the Czech Republic
Contribution summarizes the concentrations of monosaccharide anhydrides, resin acids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes, hopanes, steranes and polyaromatic ...
Development of microfluidic tools for cell analysis
Václavek, Tomáš; Křenková, Jana; Foret, František
2015 - English
In this poster we present our microfabrication approach for fabrication of glass microfluidic devices which we combine with 3D-printed parts in order to develop an easy-to-use instrumentation for single cell analysis.
Keywords:
microfluidic device; 3D-printing; single cell analysis
Fulltext is available at external website.
Development of microfluidic tools for cell analysis
In this poster we present our microfabrication approach for fabrication of glass microfluidic devices which we combine with 3D-printed parts in order to develop an easy-to-use instrumentation for ...
Determination of biological active compounds in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora; Šalplachta, Jiří
2015 - English
GC-MS and SDS-PAGE methods were employed for determination of carbohydrates and biological active proteins in pressurized hot water extract of Sambucus nigra L. branches.
Keywords:
PHWE; GC-MS; SDS-PAGE
Fulltext is available at external website.
Determination of biological active compounds in pressurized water extract of Sambucus nigra L. branches
GC-MS and SDS-PAGE methods were employed for determination of carbohydrates and biological active proteins in pressurized hot water extract of Sambucus nigra L. branches.
Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Lišková, Marcela; Křenková, Jana; Klepárník, Karel; Pazdera, P.; Foret, František
2015 - English
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on easily prepared Förster Resonance Energy Transfer (FRET). We use very simple chemistry called “click”. This type of chemistry takes advantages of quickness, simplicity and cheapness. “Click chemistry” is based on usage of various functional group and cross-linkers to combine individual molecules together.
Keywords:
caspases; click chemistry; cell analysis
Fulltext is available at external website.
Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences ...
Low-cost 3D printed device for fabrication of nanoESI tips by grinding
Přikryl, Jan; Týčová, Anna; Foret, František
2015 - English
The aim of this work was the development of a simple and low-cost device for nanospray tips fabrication. Although there are several ways of the tip preparation, grinding was chosen as the most suitable in this work. Inexpensive, flexible and reliable method of 3D printing was used for the device construction making it available for any research group with a 3D printer.
Keywords:
3D printing; grinding; nanospray tip
Fulltext is available at external website.
Low-cost 3D printed device for fabrication of nanoESI tips by grinding
The aim of this work was the development of a simple and low-cost device for nanospray tips fabrication. Although there are several ways of the tip preparation, grinding was chosen as the most ...
Quantum dot-based immunoprobe for optical and electrochemical detection
Dvořáková, V.; Čadková, M.; Datinská, Vladimíra; Chałupniak, A.; Korecká, L.; Merkoci, A.; Klepárník, Karel; Foret, František; Bílková, Z.
2015 - English
Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with following optical and electrochemical detection. This approach is essentially based on carbodiimide chemistry however each single step of whole procedure is separated and controlled which makes this applied protocol oriented, highly efficient and it enables gain of pure antibody-conjugated quantum dots in ready-to-use condition.
Keywords:
quantum dots; conjugation; carbodiimide coupling; IgG molecules
Fulltext is available at external website.
Quantum dot-based immunoprobe for optical and electrochemical detection
Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with ...
Study of the cell wall of Staphylococcus aureus and its sensitivity to enzybiotics
Čmelík, Richard; Melková, K.; Kobzová, Š.; Janda, L.
2015 - English
The endolysin resistant and sensitive strains of Staphylococcus aureus were compared by means of LC-MS based structural analysis of peptidoglycan isolated from their cell walls. The structural explanation of the resistance was suggested.
Keywords:
peptidoglycan; LC-MS; enzybiotics
Fulltext is available at external website.
Study of the cell wall of Staphylococcus aureus and its sensitivity to enzybiotics
The endolysin resistant and sensitive strains of Staphylococcus aureus were compared by means of LC-MS based structural analysis of peptidoglycan isolated from their cell walls. The structural ...
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