On-line simultaneous determination of ammonia/ammonium in ambient air
Čapka, Lukáš; Mikuška, Pavel
2019 - English
Presented paper describes methods for on-line determination of gaseous ammonia and particulate ammonium in ambient air.
Keywords:
ammonia; ammonium; CGU-ACTJU sampler
Available in digital repository of the ASCR
On-line simultaneous determination of ammonia/ammonium in ambient air
Presented paper describes methods for on-line determination of gaseous ammonia and particulate ammonium in ambient air.
LIBS assessment of spatial photon-upconversion nanoparticle distribution in model plants (R. sativus and L. minor)
Modlitbová, P.; Novotný, K.; Hlaváček, Antonín; Pořízka, P.; Kaiser, J.
2019 - English
In the present study, radish (Raphanus sativus L.) and common duckweed (Lemna minor L.) were treated with an aqueous dispersion of carboxylated silica-coated photon-upconversion nanoparticles containing rare-earth elements (Y, Yb, and Er). The total content of rare earths and their bioaccumulation factors were determined in the root, hypocotyl, and leaves of R. sativus after 72 hours, and in L. minor fronds after 168 hours. In R. sativus, translocation factors were determined as the ratio of rare earths content in hypocotyl versus roots and in leaves versus hypocotyl. The lengths of the root and hypocotyl in R. sativus, as well as the frond area in L. minor were monitored as toxicity end points. To distinguish rare-earth bioaccumulation patterns, two-dimensional maps of elemental distribution in the whole R. sativus plant and in L. minor fronds were obtained by using laser-induced breakdown spectroscopy with a lateral resolution of 100 μm. Obtained results revealed that the tested nanoparticles became adsorbed on L. minor fronds and R. sativus roots, and got transferred from roots through the hypocotyl into leaves in R. sativus. Our results show that bioaccumulation patterns and spatial distribution of rare earths in nanoparticle-treated plants differ from those of positive control (the mixture of YCl3, YbCl3, and ErCl3).
Keywords:
photonupconversion nanoparticles; luminescent nanomaterials; bioaccumulation
Available in digital repository of the ASCR
LIBS assessment of spatial photon-upconversion nanoparticle distribution in model plants (R. sativus and L. minor)
In the present study, radish (Raphanus sativus L.) and common duckweed (Lemna minor L.) were treated with an aqueous dispersion of carboxylated silica-coated photon-upconversion nanoparticles ...
Optimization of photochemical vapor generation of nickel with ICP-MS detection
Šoukal, Jakub; Musil, Stanislav
2019 - English
Photochemical vapor generation of Ni has been investigated in detail using ICP-MS as the detector.
Keywords:
photochemical vapor generation; nickel; ICP-MS
Available in digital repository of the ASCR
Optimization of photochemical vapor generation of nickel with ICP-MS detection
Photochemical vapor generation of Ni has been investigated in detail using ICP-MS as the detector.
Microfluidic device for cell counting and electrical lysis
Václavek, Tomáš; Křenková, Jana; Foret, František
2018 - English
This lecture presents two microfluidic devices as essential units for single cell proteomic analysis, firstly a device for cell counting based on resistive pulse sensing as an alternative to optical platforms based on visual control over the sample input, secondly a unit for electrical lysis of cells using direct current voltage pulses to lyse cells one by one with a possibility to analyze the distinct lysate zones by electrospray ionization mass spectrometry.
Keywords:
microfluidic devices; microextraction of basic drugs; single cell proteomic analysis; electrospray ionization mass spectrometry; cell lysis
Fulltext is available at external website.
Microfluidic device for cell counting and electrical lysis
This lecture presents two microfluidic devices as essential units for single cell proteomic analysis, firstly a device for cell counting based on resistive pulse sensing as an alternative to optical ...
Impact of post-harvest processing, geographical locality and production year on quality of selected medicinal plants
Burdějová, Lenka; Polovka, M.; Pořízka, J.
2018 - English
This study deals with the assessment of the impact of post-harvest treatment, geographical location and production years on 32 selected characteristics of medicinal plants grown in the Czech Republic. Dimethyl sulfoxide herbal extracts characteristics were evaluated by spectroscopic and chromatographic techniques. The results were processed by analysis of variance and multivariate statistical tools. Obtained results clearly proved the successful differentiation of herbal samples by means of canonical discrimination analysis according to post-harvest treatment (100%), geographical origin (82.9%) and production year (96.1%) confirming that studied factors may have significant effect on quality of medicinal plants.
Keywords:
HPLC-DAD; ICP-OES; plants extracts; herbal extracts
Fulltext is available at external website.
Impact of post-harvest processing, geographical locality and production year on quality of selected medicinal plants
This study deals with the assessment of the impact of post-harvest treatment, geographical location and production years on 32 selected characteristics of medicinal plants grown in the Czech Republic. ...
A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D
Ďurč, P.; Lačná, J.; Dosedělová, V.; Foret, František; Homoľa, L.; Pokojová, E.; Malá, M.; Skřičková, J.; Dastych, M.; Vinohradská, H.; Dřevínek, P.; Skalická, V.; Kubáň, Petr
2018 - English
A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. The collected sample is analyzed by capillary electrophoresis with contactless conductometric detection (CE-C4D). Ion ratios are determined as ratios of peak area of chloride anion and peak areas of selected cations. It is possible to distinguish between cystic fibrosis patients and healthy individuals when ion ratio is applied as diagnostic factor. In here, we present analytical parameters of developed method (limits of detection, limits of quantification, repeatability of migration times, repeatability of peak areas) and ROC curve, which reveals high sensitivity and specificity of the developed method.
Keywords:
method for diagnostic of cystic fibrosis; cystic fibrosis; capillary electrophoresis with contactless conductometric detection
Fulltext is available at external website.
A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D
A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. ...
Acetonitrile-assisted enzymatic digestion used for analysis of proteins of cancer origin
Laštovičková, Markéta; Bobál, P.; Strouhalová, Dana; Bobálová, Janette
2018 - English
In this study, in-gel trypsin digestion protocol using aqueous-acetonitrile solvent system was developed to facilitate bottom-up proteomic identification of proteins isolated from human breast cancer cells.
Keywords:
breast cancer cells; enzymatic digestion; MS analysis
Fulltext is available at external website.
Acetonitrile-assisted enzymatic digestion used for analysis of proteins of cancer origin
In this study, in-gel trypsin digestion protocol using aqueous-acetonitrile solvent system was developed to facilitate bottom-up proteomic identification of proteins isolated from human breast cancer ...
Droplet-based microfluidic chip with passive mixer for analysis with photon-upconversion nanoparticles
Křivánková, Jana; Přikryl, Jan; Hlaváček, Antonín
2018 - English
Mixing of the fluids in microfluidic chips is important especially in applications, where droplet contents need to be rapidly homogenized. Here, a passive method for mixing in polydimethylsiloxane (PDMS) microchannels is presented. Droplet homogenization was firstly tested with organic stains and subsequently applied for mixing water dispersions of photon-upconversion nanoparticles (UCNPs).\n
Keywords:
microfluidic chips; microfluidic systems; photon-upconversion nanoparticles
Fulltext is available at external website.
Droplet-based microfluidic chip with passive mixer for analysis with photon-upconversion nanoparticles
Mixing of the fluids in microfluidic chips is important especially in applications, where droplet contents need to be rapidly homogenized. Here, a passive method for mixing in polydimethylsiloxane ...
Supercritical water in etching and morphing of siliceous surfaces for analytical separations
Karásek, Pavel; Horká, Marie; Planeta, Josef; Roth, Michal
2018 - English
Applications of supercritical water in etching and morphing of siliceous surfaces for analytical separations are briefly reviewed.
Keywords:
supercritical water; fused silica capillary; electromigration methods
Available in digital repository of the ASCR
Supercritical water in etching and morphing of siliceous surfaces for analytical separations
Applications of supercritical water in etching and morphing of siliceous surfaces for analytical separations are briefly reviewed.
Capillary electrophoresis in clinical diagnosis and monitoring
Kubáň, Petr
2018 - English
There is a significant potential of CE to provide a useful analytical tool to analyze noninvasive samples of body fluids and stimulate the cooperation between analytical chemists and clinicians in this interdisciplinary field.
Keywords:
CE; CE-LIF; clinical diagnosis
Available in digital repository of the ASCR
Capillary electrophoresis in clinical diagnosis and monitoring
There is a significant potential of CE to provide a useful analytical tool to analyze noninvasive samples of body fluids and stimulate the cooperation between analytical chemists and clinicians in ...
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