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Prototyping of a simple device for parallel dot blotting using CNC machining
Novotný, Jakub; Svobodová, Z.; Bílková, Z.; Foret, František
2016 - English
Computer numerical control (CNC) machining has been tested for rapid prototyping of\na new design of a dot-blotting device suitable for parallel testing of antibodies. The\ndevice, machined from polycarbonate, was prepared using an inexpensive computer\ncontrolled CNC router.
Keywords:
CNC machines; dot-blotters; CNC router
Available in digital repository of the ASCR
Prototyping of a simple device for parallel dot blotting using CNC machining
Computer numerical control (CNC) machining has been tested for rapid prototyping of\na new design of a dot-blotting device suitable for parallel testing of antibodies. The\ndevice, machined from ...
Evaluation of stationary phases for HPLC based on nanofibers
Čmelík, Richard; Foret, František
2016 - English
In this study, the performance parameters were compared for the set of chromatographic columns filled with polyacrylonitrile nanofibers processed in the different manner. The advantages and challenges of the novel fillings were discussed.
Keywords:
nanofiber; HPLC; stationary phase
Available in digital repository of the ASCR
Evaluation of stationary phases for HPLC based on nanofibers
In this study, the performance parameters were compared for the set of chromatographic columns filled with polyacrylonitrile nanofibers processed in the different manner. The advantages and challenges ...
Geochemistry of Cenozoic volcanic rocks of the Faroe Islands with a focus on their ultra-trace contents of Hg
Šišková, P.; Krmíček, Lukáš; Coufalík, Pavel
2016 - English
According to geophysical research performed in the eastern part of the North Atlantic, many authors inferred that the basalt flows on the Faroe Islands overlie a continental metamorphic basement supposedly of Precambrian age. With respect to this, we focused our study on determination of ultratrace mercury contents by means of atomic absorption spectrometry to test this hypothesis. Mercury contents in basalts seem to be a sensitive tool for distinguishing between the basalts not affected by interaction with the continental crust and the basalts enclosing crustal xenoliths. Contents of Hg show great variability between samples even within the same formation. We presume that high concentrations of Hg in some samples are related to stagnation of individual pulses of parent magma in the continental crust below the Faroe Islands.
Keywords:
Faroe Islands; tholeiitic basalt; mercury content; continental crust
Available in digital repository of the ASCR
Geochemistry of Cenozoic volcanic rocks of the Faroe Islands with a focus on their ultra-trace contents of Hg
According to geophysical research performed in the eastern part of the North Atlantic, many authors inferred that the basalt flows on the Faroe Islands overlie a continental metamorphic basement ...
Concentration of atomic hydrogen measured in a heated atomizer by two-photon absorption laser-induced fluorescence
Dvořák, P.; Talába, M.; Dědina, Jiří; Kratzer, Jan; Obrusník, A.
2016 - English
Concentration of free hydrogen atoms was measured by the two-photon absorption laser-induced fluoresence (TALIF) in an externally heated atomizer in a mixture of argon, hydrogen and oxygen at atmospheric pressure. The atomic hydrogen concentration in the order 10^22 m^-3 was found in the region around the flame.
Keywords:
TALIF; quartz tube atomizer; hydrogen measurement
Available in digital repository of the ASCR
Concentration of atomic hydrogen measured in a heated atomizer by two-photon absorption laser-induced fluorescence
Concentration of free hydrogen atoms was measured by the two-photon absorption laser-induced fluoresence (TALIF) in an externally heated atomizer in a mixture of argon, hydrogen and oxygen at ...
Arsenic speciation by hydride generation coupled with atomic fluorescence spectrometry
Marschner, Karel; Musil, Stanislav; Rychlovský, P.; Dědina, Jiří
2016 - English
Post-column hydride generation for arsenic speciation was used and the demethylation of Arsenic species during hydride generation was investigated.
Keywords:
arsenic speciation; demethylation; hydride generation
Available in digital repository of the ASCR
Arsenic speciation by hydride generation coupled with atomic fluorescence spectrometry
Post-column hydride generation for arsenic speciation was used and the demethylation of Arsenic species during hydride generation was investigated.
CE-nESI/MS in electrode-free design: narrowing the separation channel to 5 μm
Týčová, Anna; Foret, František
2016 - English
Capillary electrophoresis-nanospray/mass spectrometry (CE-nESI/MS) in electrodefree\narrangement represents a very simple instrumentation for analysis of biomolecules.\nSo far, CE-nESI/MS analyses in this design were limited to 10-75 μm ID of the\nseparation channel. Although the work with narrower dimensions brings several\nchallenges, there is a great potential for better sensitivity, improved separation power\nand lower sample consumption. This work is devoted to some practical aspects of\nexperiments in narrow bore channels and systematic evaluation of CE-nESI/MS\nanalyses conducted in capillaries of 25, 15 and 5 μm ID.
Keywords:
CE-nESI/MS; electrode-free arragement
Available in digital repository of the ASCR
CE-nESI/MS in electrode-free design: narrowing the separation channel to 5 μm
Capillary electrophoresis-nanospray/mass spectrometry (CE-nESI/MS) in electrodefree\narrangement represents a very simple instrumentation for analysis of biomolecules.\nSo far, CE-nESI/MS analyses in ...
Comparative evaluation of protein composition in human breast cancer cells using mass spectrometry
Flodrová, Dana; Toporová, L.; Macejová, D.; Laštovičková, Markéta; Brtko, J.; Bobálová, Janette
2016 - English
Bottom-up proteomic approach was used for detailed characterization of proteins from\ntwo human tumour cell lines representing major clinically different types of breast\ncancer. The aim was to show the differences between them on proteomic level. Here\nwe present almost 100 unequivocally identified proteins out of which 60 were mutually\ndifferently expressed for MDA-MB-231 and MCF-7. Some well-known breast cancer\nmarkers like annexins A1, A2 and vimentin were found in the MDA-MB-231 cell line.\nOn the other hand, MCF-7 cells were found to be positive for cytokeratins and keratins\nand thus we were able to distinguish both cell lines sufficiently.
Keywords:
mass spectrometry; breast cancer; human tumour; bottom-up proteomic approach
Available in digital repository of the ASCR
Comparative evaluation of protein composition in human breast cancer cells using mass spectrometry
Bottom-up proteomic approach was used for detailed characterization of proteins from\ntwo human tumour cell lines representing major clinically different types of breast\ncancer. The aim was to show ...
Hexahistidine labeling of oligosaccharides
Partyka, Jan; Křenková, Jana; Foret, František
2016 - English
We have presented another approach for labeling of oligosaccharides suitable for\ncapillary zone electrophoresis coupled with mass spectrometric detection.\nOligosaccharides labeled by peptides with hexahistidine sequence have faster\nseparation resulting in two or three times shorter time in comparison to oligosaccharides\nwith singly charged labels.
Keywords:
capillary zone electrophoresis; mass spectrometric detection; oligosaccharides
Available in digital repository of the ASCR
Hexahistidine labeling of oligosaccharides
We have presented another approach for labeling of oligosaccharides suitable for\ncapillary zone electrophoresis coupled with mass spectrometric detection.\nOligosaccharides labeled by peptides with ...
Organic compounds in PM1 aerosol in the Central Bohemian Region in the Czech Republic
Křůmal, Kamil; Mikuška, Pavel; Coufalík, Pavel; Večeřa, Zbyněk
2015 - English
Contribution summarizes the concentrations of monosaccharide anhydrides, resin acids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes, hopanes, steranes and polyaromatic hydrocarbons in PM1 aerosols in small town Celakovice in winter. According to concentrations of organic markers, the identification of emission sources in Celakovice is discussed.
Keywords:
organic tracer; urban aerosol; PM1
Fulltext is available at external website.
Organic compounds in PM1 aerosol in the Central Bohemian Region in the Czech Republic
Contribution summarizes the concentrations of monosaccharide anhydrides, resin acids, methoxyphenols, monosaccharides, disaccharides, sugar alcohols, alkanes, hopanes, steranes and polyaromatic ...
Development of microfluidic tools for cell analysis
Václavek, Tomáš; Křenková, Jana; Foret, František
2015 - English
In this poster we present our microfabrication approach for fabrication of glass microfluidic devices which we combine with 3D-printed parts in order to develop an easy-to-use instrumentation for single cell analysis.
Keywords:
microfluidic device; 3D-printing; single cell analysis
Fulltext is available at external website.
Development of microfluidic tools for cell analysis
In this poster we present our microfabrication approach for fabrication of glass microfluidic devices which we combine with 3D-printed parts in order to develop an easy-to-use instrumentation for ...
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