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Analysis of starch degradation products and other carbohydrates from spruce needles using various analytical techniques
Cabálková, J.; Chmelík, Josef
2004 - English
Starch degradation products were analysed by AsFlFFF-MALS-RI and SEC-MALS-RI for determination of molar mass distribution. Separation of low molecular weight fractions of carbohydrates using HPLC-RI led to the identification of various mono-, di-, tri and tetrasaccharides. MALDI-TOF MS spectra showed the presence of higher oligosaccharides Degradační produkty škrobu byly analyzovány pomocí AsFlFFF-MALS-RI a SEC-MALS-RI a stanoveny distribuce molekulových hmotností. Separace nízkomolekulárních frakcí karbohydrátů pomocí HPLC-RI vedla k identifikaci různých mono-, di-, tri a tetrasacharidů. MALDI-TOF MS spektra prokázala přítomnost vyšších oligosacharidů
Keywords:
Norway spruce; needles; carbohydrates
Available at various institutes of the ASCR
Analysis of starch degradation products and other carbohydrates from spruce needles using various analytical techniques
Starch degradation products were analysed by AsFlFFF-MALS-RI and SEC-MALS-RI for determination of molar mass distribution. Separation of low molecular weight fractions of carbohydrates using HPLC-RI ...
Determination of carbohydrates in vegetables by MALDI-TOF mass spectrometry
Štikarovská, Markéta; Chmelík, Josef
2003 - English
MALDI-TOF MS was used for characterization of oligosaccharides in samples of Jerusalem artichoke and red onion. Seven selected matrices were tested. The optimization of experimental conditions was carried out for three carbohydrates that are important for food chemistry ů dextran, inulin and starch (low glucose syrup).
Keywords:
Oligosaccharides ů food; MALDI-TOF MS
Available at various institutes of the ASCR
Determination of carbohydrates in vegetables by MALDI-TOF mass spectrometry
MALDI-TOF MS was used for characterization of oligosaccharides in samples of Jerusalem artichoke and red onion. Seven selected matrices were tested. The optimization of experimental conditions was ...
New developments in hydride atomizers for atomic absorption and atomic fluorescence spectrometry
Dědina, Jiří; Korunová, Vlasta; D'Ulivo, A.
2003 - English
The most recent developments of multiatomizers and FIFS were described and performance of the atomizers namely in terms of resistance to atomization interferences and of minimization of the limit detection for AAS and AFS was discussed.
Keywords:
atomic absortion spectrometry; hydride atomization; hydride generation
Available at various institutes of the ASCR
New developments in hydride atomizers for atomic absorption and atomic fluorescence spectrometry
The most recent developments of multiatomizers and FIFS were described and performance of the atomizers namely in terms of resistance to atomization interferences and of minimization of the limit ...
Dynamic modification of microorganisms by pyrene-butanoate for fluorometric detection in capillary electrokinetic techniques
Horká, Marie; Růžička, F.; Šlais, Karel
2003 - English
Pyrenebutanoate and non-ionogenic tenside on the basis of pyrenebutanoate as the fluorescent compounds were used as buffer additives in capillary zone electrophoresis or capillary isoelectric focusing, respectively, for a dynamic modification of the microbial samples. Using the deuterium lamp UV excitation for the on column fluorometric detection, the minimum detectable amount of the microorganisms in order of ones to tens of cells sampled on the separation capillary was achieved.
Keywords:
capillary electrokinetic techniques; dynamic modification of microorganisms; fluorometric detection
Available at various institutes of the ASCR
Dynamic modification of microorganisms by pyrene-butanoate for fluorometric detection in capillary electrokinetic techniques
Pyrenebutanoate and non-ionogenic tenside on the basis of pyrenebutanoate as the fluorescent compounds were used as buffer additives in capillary zone electrophoresis or capillary isoelectric ...
Preparation and optimization of packing of microcolumns for HPLC and SFC
Planeta, Josef
2003 - English
The lecture summarizes the study of the influence of packing conditions on the efficiency and the total porosity of the prepared columns. For both critical and supercritical CO2, optimum packing conditions were found.and their reproducibility was defined.
Keywords:
packing microcolumns; preparation
Available at various institutes of the ASCR
Preparation and optimization of packing of microcolumns for HPLC and SFC
The lecture summarizes the study of the influence of packing conditions on the efficiency and the total porosity of the prepared columns. For both critical and supercritical CO2, optimum packing ...
Autoradiography of 75Se on Image Plate
Korunová, Vlasta
2002 - English
The Image Plate and BAS5000 Fuji Film instrument were used to depict a flat layout of 75Se and for its quantitative determination. The goal for this experiment was determinig the efficiency and area of selenium deposited during in situ trapping in the AAS graphite furnace. S graphite furnace.
Keywords:
autoradiography; 75Se isotop; image plate
Available at various institutes of the ASCR
Autoradiography of 75Se on Image Plate
The Image Plate and BAS5000 Fuji Film instrument were used to depict a flat layout of 75Se and for its quantitative determination. The goal for this experiment was determinig the efficiency and area ...
Chiral separation of charged cluster boranes
Slavíček, V.; Vespalec, Radim
2002 - English
Capillary zone electrophoresis is effective instrumental technique for chiral separations of charged cluster boranes if β-cyclodextrin serves as chiral selector.
Keywords:
capillary electrophoresis; chiral separation; cluster boranes; borany; odlučování
Available at various institutes of the ASCR
Chiral separation of charged cluster boranes
Capillary zone electrophoresis is effective instrumental technique for chiral separations of charged cluster boranes if β-cyclodextrin serves as chiral selector.
Single cell diagnostics
Klepárník, Karel
2002 - English
DNA fragmentation, as a hallmark of apoptosis, has been detected in a single MCF7 breast cancer apoptotic cell.
Keywords:
single cell analysis; apoptosis
Available at various institutes of the ASCR
Single cell diagnostics
DNA fragmentation, as a hallmark of apoptosis, has been detected in a single MCF7 breast cancer apoptotic cell.
pK determination of sparingly soluble weak bases
Vlčková, Markéta; Barták, P.; Vespalec, Radim
2002 - English
pK values of sanguinarine and chelerythrine have been determinedat conditions of planned interaction measurements and renasons for the pread of published pK data of these weak bases have been investigated.
Keywords:
capillary electrophoresis; sanguinarine; chelerythrine; zásady; elektroforéza
Available at various institutes of the ASCR
pK determination of sparingly soluble weak bases
pK values of sanguinarine and chelerythrine have been determinedat conditions of planned interaction measurements and renasons for the pread of published pK data of these weak bases have been ...
Exact solution to the Navier-Stokes equation describing the velocity profile of electroosmotic flow in cylindrical capillaries
Otevřel, Marek; Klepárník, Karel
2002 - English
The partial differential equation describing unsteady EOF velocity profile was derived and solved. The steady state EOF was solved for an electrolyte of nonconstant viscosity.
Keywords:
electroosmotic flow; Navier-Stokes equation
Available at various institutes of the ASCR
Exact solution to the Navier-Stokes equation describing the velocity profile of electroosmotic flow in cylindrical capillaries
The partial differential equation describing unsteady EOF velocity profile was derived and solved. The steady state EOF was solved for an electrolyte of nonconstant viscosity.
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