Number of found documents: 405
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Influence of stringent response in Corynebacterium glutamicum on valine production
Prouza, Marek; Holátko, Jiří; Elišáková, Veronika; Nešvera, Jan; Pátek, Miroslav
2006 - English
The aim of this study was to compare the production of valine in C. glutamicum producers with the level of valine produced by the strain with deletion of the rel gene coding for enzyme guanosine tetraphosphate synthetase Cílem práce bylo porovnat tvorbu valinu u producentů C. glutamicum s tvorbou valinu u kmene s delecí genu rel, který kóduje enzym guanosintetrafosfátsynthetasu Keywords: corynebacterium glutamicum; guanosine tetraphosphate synthetase Available at various institutes of the ASCR
Influence of stringent response in Corynebacterium glutamicum on valine production

The aim of this study was to compare the production of valine in C. glutamicum producers with the level of valine produced by the strain with deletion of the rel gene coding for enzyme guanosine ...

Prouza, Marek; Holátko, Jiří; Elišáková, Veronika; Nešvera, Jan; Pátek, Miroslav
Mikrobiologický ústav, 2006

Stimulation effects of gliadin to human dendritic cells
Pecharová, Barbara; Palová-Jelínková, Lenka; Rožková, D.; Bártová, J.; Šedivá, A.; Tlaskalová, Helena; Spíšek, R.; Tučková, Ludmila
2006 - English
This work studies the stimulatins effects of gliadin to human dendritic cells Tato práce se zabývá výzkumem stimulačního efektu gliadinu na lidské dendritické buňky Keywords: dendritic cells; antigen; gliadin peptides Available at various institutes of the ASCR
Stimulation effects of gliadin to human dendritic cells

This work studies the stimulatins effects of gliadin to human dendritic cells


Tato práce se zabývá výzkumem stimulačního efektu gliadinu na lidské dendritické buňky

Pecharová, Barbara; Palová-Jelínková, Lenka; Rožková, D.; Bártová, J.; Šedivá, A.; Tlaskalová, Helena; Spíšek, R.; Tučková, Ludmila
Mikrobiologický ústav, 2006

Investigation of genes involved in phenol and naphthalene degradation in Rhodococcus erythropolis CCM2595 and Rhodococcus sp. NCIMB12038
Knoppová, Monika; Kulakov, L. A.; Larkin, M. J.; Pátek, Miroslav
2006 - English
The strain R. erythropolis CCM2595 is able to degrade phenol by the beta-ketoadipate pathway and the strain Rhodococcus sp. NCIMB12038 is able to degrade naphthalene via gentisate. Enzyme studies of these strains indicate that strain NCIMB12038 also contains enzymes necessary for phenol utilization and strain CCM2595 can also degrade naphthalene. Pulse field gel electrophoresis was used and southern hybridisation experiments were performed to reveal localization the genes involved in phenol and naphthalene degradation in both strains Kmen R. erythropolis CCM2595 se vyznačuje schopností degradovat fenol keto-adipátovou dráhou a kmen Rhodococcus sp. NCIMB12038 je schopen degradovat naftalen dráhou gentisátovou. Enzymové studie těchto kmenů ukazují, že kmen NCIMB12038 nese také geny nezbytné pro degradaci fenolu a kmen CCM2595 také pro degradaci naftalenu. Pulsní gelovou elektroforézou a southern hybridizací jsme lokalizovali geny podílející se na degradaci fenolu a naftalenu v obou kmenech Keywords: phenol; rhodococcus erythropolis Available at various institutes of the ASCR
Investigation of genes involved in phenol and naphthalene degradation in Rhodococcus erythropolis CCM2595 and Rhodococcus sp. NCIMB12038

The strain R. erythropolis CCM2595 is able to degrade phenol by the beta-ketoadipate pathway and the strain Rhodococcus sp. NCIMB12038 is able to degrade naphthalene via gentisate. Enzyme studies of ...

Knoppová, Monika; Kulakov, L. A.; Larkin, M. J.; Pátek, Miroslav
Mikrobiologický ústav, 2006

In depth study of physiological roles of TIF34/eIF3i and TIF35/eIF3g subunits of yeast
Janoušková, Olga; Nielsen, K. H.; Valášek, Leoš
2006 - English
Translation initiation is a multiple-step process involving a large number of soluble eukaryotic initiation factors that ultimately leads to formation of the 80S ribosome with Met-tRNAiMet based paired with the start AUG codon of mRNA. Among the initiation factors, eIF3 draws a special attention since it was shown to promote several critical steps such as recruitment of the eIF2GTPMet-tRNAiMet ternary complex and mRNA to the 40S ribosomal subunit and was implicated in cancer Práce pojednáva o studiu inciace translace a specifické úloze translačního iniciačního faktoru eIF3 Keywords: eukaryotic translation; yeast Available at various institutes of the ASCR
In depth study of physiological roles of TIF34/eIF3i and TIF35/eIF3g subunits of yeast

Translation initiation is a multiple-step process involving a large number of soluble eukaryotic initiation factors that ultimately leads to formation of the 80S ribosome with Met-tRNAiMet based ...

Janoušková, Olga; Nielsen, K. H.; Valášek, Leoš
Mikrobiologický ústav, 2006

New built bioreactor control systems for Bioengineering equipment
Prell, Aleš; Sobotka, Miroslav; Kujan, Petr; Šafář, Hynek; Holler, Pavel
2006 - English
In cooperation of the automation company Proteco Pardubice the complete replacement of classic analogue control units of next two bioreactors by new, PLC driven local control systems for all monitored and controlled parameters was done. Some measuring devices and actuators were substituted by a new technique too and, moreover, a new central SCADA/HMI (Reliance, Geovap Pardubice) system of the set of bioreactors was introduced and the remaining analogue driven fermentors were integrated into it Ve spolupráci s automatizační společností Proteco Pardubice byla provedena kompletní výměna analogových řídících jednotek bioreaktorů a vybudovány nové lokální a centrální řídící systémy založené na PLC Tecomat a SCADA/HMI Reliance, Geovap Pardubice. Zbývající analogové řídící systémy byly integrovány Keywords: fermenter control; monitoring Available at various institutes of the ASCR
New built bioreactor control systems for Bioengineering equipment

In cooperation of the automation company Proteco Pardubice the complete replacement of classic analogue control units of next two bioreactors by new, PLC driven local control systems for all monitored ...

Prell, Aleš; Sobotka, Miroslav; Kujan, Petr; Šafář, Hynek; Holler, Pavel
Mikrobiologický ústav, 2006

Secondary metabolites in insect associated Geosmithia species: polyhydroxylated anthraquinones of G. lavendula and perspectives
Kolařík, Miroslav; Stodůlková, Eva; Šulc, Miroslav; Kuzma, Marek; Man, Petr; Flieger, Miroslav; Křesinová, Zdena
2006 - English
Cultures of G. lavendula produce various colored hydroxylated antraquinones (AQ). Rhodolamprometrin and pentahydroxy-AQ were identified so far. Natural AQs are used as dyes and pharmaceuticals Kultury G. lavendula produkují řadu barevných hydroxylovaných antrachinonů (AQ). Dosud jsme identifikovali rhodolamprometrin and pentahydroxy-AQ. Přírodní AQ jsou využívány jako barviva a léčiva Keywords: geosmithia lavendula; hydroxylated antraquinones Available at various institutes of the ASCR
Secondary metabolites in insect associated Geosmithia species: polyhydroxylated anthraquinones of G. lavendula and perspectives

Cultures of G. lavendula produce various colored hydroxylated antraquinones (AQ). Rhodolamprometrin and pentahydroxy-AQ were identified so far. Natural AQs are used as dyes and ...

Kolařík, Miroslav; Stodůlková, Eva; Šulc, Miroslav; Kuzma, Marek; Man, Petr; Flieger, Miroslav; Křesinová, Zdena
Mikrobiologický ústav, 2006

The effect of isoleucine limitation on valine precursor levels and acetohydroxyacid synthase activity in stringent (rel+) and relaxed (rel-) Corynebacterium glutamicum mutant
Denina, I.; Paegle, L.; Ruklisha, M.; Prouza, Marek; Pátek, Miroslav
2006 - English
Isoleucine limitation was found to increase acetohydroxyacid synthase activity in Corynebacterium glutamicum cells. Similar increase of activity of this enzyme was observed in C. glutamicum rel+ strains as well in rel- mutant Bylo zjištěno, že limitace isoleucinem vede ke zvýšení aktivity synthasy acetohydroxykyselin v buňkách Corynebacterium glutamicum. Podobné zvýšení aktivity tohoto enzymu bylo pozorováno u kmene C. glutamicum rel+ i u mutanty rel- Keywords: isoleucine; corynebacterium glutamicum Available at various institutes of the ASCR
The effect of isoleucine limitation on valine precursor levels and acetohydroxyacid synthase activity in stringent (rel+) and relaxed (rel-) Corynebacterium glutamicum mutant

Isoleucine limitation was found to increase acetohydroxyacid synthase activity in Corynebacterium glutamicum cells. Similar increase of activity of this enzyme was observed in C. glutamicum rel+ ...

Denina, I.; Paegle, L.; Ruklisha, M.; Prouza, Marek; Pátek, Miroslav
Mikrobiologický ústav, 2006

The Influence of Calcium to Calmodulin-Melittin Complex: A study Using Chemical Gross-linking and FTMS
Giannakopulos, A.; Derrick, P. J.; Havlíček, Vladimír; Novák, Petr
2006 - English
Melittin binds tightly to calmodulin in the presence of calcium and inhibits the function o f calmodulin. Chemical cross-linking combined with mass spectrometry has been used in order to define the positions of Calmodulin and melittin in the complex at different concentrations of calcium Melittin se váže pevně kalmodulin v přítomnosti vápníku a inhibuje jeho funkci. Chemické zesítění a hmotnostní spektrometrie byly použity za účlem nalezení místa intrakce mezi kalmodulinem a melittin za ruzných koncentrací vápníku Keywords: mass spectrometry; chemical cross-linking Available at various institutes of the ASCR
The Influence of Calcium to Calmodulin-Melittin Complex: A study Using Chemical Gross-linking and FTMS

Melittin binds tightly to calmodulin in the presence of calcium and inhibits the function o f calmodulin. Chemical cross-linking combined with mass spectrometry has been used in order to define the ...

Giannakopulos, A.; Derrick, P. J.; Havlíček, Vladimír; Novák, Petr
Mikrobiologický ústav, 2006

Evaluation of interactions between lectin-like receptors using glycodendrimers
Vannucci, Luca; Pospíšil, Miloslav; Rossmann, Pavel; Chernyavskiy, Oleksandr; Kubínová, Lucie; Křen, Vladimír; Luptovcová, Martina; Svoboda, Jan; Kuldová, Markéta; Bezouška, Karel; Fišerová, Anna
2006 - English
The events following the administration of GN8 in vitro, in vivo and ex vivo, lead to increased expression of CD69. The CD69-dependent IFNg production is modulated by co-activation of NKG2D and Ly49 receptors. Glycodendrimers can assist the dissection of activation network of NK/NKT cell lectin-like receptors in rat and murine models Podání GN8 v in vitro, in vivo a ex vivo podmínkách zvyšuje expresi CD69 antigenu. CD69-dependentní produkce IFNg je ovlivněna rovněž aktivací NKG2D a Ly49 receptorů. Glykodendrimery jsou vhodné pro studium NK/NKT buněčné aktivační sítě lektinových receptorů v potkaních a myších modelech Keywords: glycodendrimers; lectin-like receptors; cytotoxic lymphocytes Available at various institutes of the ASCR
Evaluation of interactions between lectin-like receptors using glycodendrimers

The events following the administration of GN8 in vitro, in vivo and ex vivo, lead to increased expression of CD69. The CD69-dependent IFNg production is modulated by co-activation of NKG2D and Ly49 ...

Vannucci, Luca; Pospíšil, Miloslav; Rossmann, Pavel; Chernyavskiy, Oleksandr; Kubínová, Lucie; Křen, Vladimír; Luptovcová, Martina; Svoboda, Jan; Kuldová, Markéta; Bezouška, Karel; Fišerová, Anna
Mikrobiologický ústav, 2006

Analýza přítomnosti cytokinů v mateřském mléku a kolostru pomocí protilátkového mikročipu
Kverka, Miloslav; Burianová, Jaroslava; Lodinová-Žádniková, R.; Kocourková, I.; Tučková, Ludmila; Tlaskalová, Helena
2006 - Czech
Přednáška pojednává o použití protilátkového mikročipu při analýze cytokinového spektra mateřského mléka a kolostra. Blíže se zabývá jejich biologickým významem pro kojené dítě The lecture discuss use of the antibody array in cytokine spectra analysis in human milk. The role of the newly discovered cytokines for the suckling is discussed Keywords: human colostrum; milk; cytokines Available at various institutes of the ASCR
Analýza přítomnosti cytokinů v mateřském mléku a kolostru pomocí protilátkového mikročipu

Přednáška pojednává o použití protilátkového mikročipu při analýze cytokinového spektra mateřského mléka a kolostra. Blíže se zabývá jejich biologickým významem pro kojené dítě...

Kverka, Miloslav; Burianová, Jaroslava; Lodinová-Žádniková, R.; Kocourková, I.; Tučková, Ludmila; Tlaskalová, Helena
Mikrobiologický ústav, 2006

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