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MORPHOLOGICAL CLUSTERING OF INTERICTAL EPILEPTIFORM DISCHARGES IN INTRACRANIAL ELECTROENCEPHALOGRAPHY
Vlk, Pavel; Janča, R.; Čmejla, R.; Kršek, P.; Marusič, P.; Jiruška, Přemysl
2013 - English
To improve the localizing yield of interictal discharges, identification of specific features of the discharges generated only within epileptogenic region is required. We have developed self-clustering algorithm which discriminates distinct populations of interictal epileptiform discharges according to the morphology of their waveforms. Results demonstrate the ability of the algorithm to separate interictal epileptiform discharges according to their morphological features
Keywords:
epilepsy; interictal epiletiform discharges; morphological clustering
Available at various institutes of the ASCR
MORPHOLOGICAL CLUSTERING OF INTERICTAL EPILEPTIFORM DISCHARGES IN INTRACRANIAL ELECTROENCEPHALOGRAPHY
To improve the localizing yield of interictal discharges, identification of specific features of the discharges generated only within epileptogenic region is required. We have developed ...
ANALYSIS OF NON-ENZYMATIC POSTTRANSLATIONAL MODIFICATED (GLYCATED) ALBUMIN BY NANO-LC/MS/MS
Šťastná, Zdeňka; Pataridis, Statis; Sedláková, Pavla; Mikšík, Ivan
2012 - English
Posttranslational modifications of proteins are important reactions, which significantly affect the function of proteins in the organism. In principle, they can be divided into enzymatic and non-enzymatic modifications. Non-enzymatic reactions include glycation (earlier called nonezymatic glycosylation), which plays an important role in the development of chronic complications of diabetes mellitus, uremia, in the process of aging and degeneration of the brain.This work deals with the study of glycated albumins (human serum albumin and bovine serum albumin). Methodologically we used nano-liquid chromatography coupled to Q-TOF mass spectrometer. In vitro modified proteins were cleavaged by trypsin and arising peptides were separated on C18 nano column with trap-column. Peptides and their modifications were analysed by high-resolution Q-TOF mass spectrometer MaXis with precision determination of mass below 2 ppm. We found some modifications of proteins. Besides well known carboxymethyllysine new ones were determined - create mass shift 78, 132 and 218. Origin of these modifications is discussed and possible structure is presented. All found modifications were allocated to the structure of proteins and reactivity to various oxo-compounds was also examined
Keywords:
non-enzymatic glycation; serum albumin
Available at various institutes of the ASCR
ANALYSIS OF NON-ENZYMATIC POSTTRANSLATIONAL MODIFICATED (GLYCATED) ALBUMIN BY NANO-LC/MS/MS
Posttranslational modifications of proteins are important reactions, which significantly affect the function of proteins in the organism. In principle, they can be divided into enzymatic and ...
Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images
Michálek, Jan; Čapek, Martin; Mao, X. W.; Kubínová, Lucie
2010 - English
Due to multiple distortion effects, confocal laser scanning microscopy (CLSM) images of even homogeneous specimen regions suffer from irregular brightness variations. In this paper, a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain, is presented. The method does not require any special calibration (reference) images since the gain estimate is extracted from the CLSM image being corrected itself. The proposed approach exploits two types of morphological filters: the median filter and a morphological operator called the upper Lipschitz cover
Keywords:
CLSM; illumination inhomogeneities
Available at various institutes of the ASCR
Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images
Due to multiple distortion effects, confocal laser scanning microscopy (CLSM) images of even homogeneous specimen regions suffer from irregular brightness variations. In this paper, a fast correction ...
Vývoj radiometrických metod pro stanovení enzymových aktivit jodothyronin dejodas: Vliv antidepresiva fluoxetinu na aktivity dejodas typu 1,2 a 3 v mozku potkana
Pavelka, Stanislav
2010 - Czech
Účelem předkládané práce bylo zjištění experimentálních podmínek a vypracování korektních metod pro radiometrická měření enzymových aktivit jodothyronin dejodas typu 1, 2 a 3. Specielně pak použití těchto vypracovaných metod pro sledování účinků antidepresiva fluoxetinu (Prozac) na metabolismus tyreoidálních hormonů u laboratorního potkana The objectives of the present study were to establish valid assay conditions for measurement of the types 1, 2 and 3 iodothyronine deiodinases activities, and to use the radiometric enzyme assays especially to follow the effects of antidepressant drug fluoxetine (Prozac) on the metabolism of thyroid hormones in the rat
Keywords:
antidepressant drug; deiodinase; radiometric assay
Available at various institutes of the ASCR
Vývoj radiometrických metod pro stanovení enzymových aktivit jodothyronin dejodas: Vliv antidepresiva fluoxetinu na aktivity dejodas typu 1,2 a 3 v mozku potkana
Účelem předkládané práce bylo zjištění experimentálních podmínek a vypracování korektních metod pro radiometrická měření enzymových aktivit jodothyronin dejodas typu 1, 2 a 3. Specielně pak použití ...
Fitting geometrical models to 3D confocal microscopy images using graph cuts iteratively
Janáček, Jiří
2010 - English
A new iterative method for fitting triangulated surfaces and geometric graphs to surfaces and fibre-like structures is based on the calculation of the minimal graph cut in every iteration. The method was used for improvement of the geometric model of muscle fibres surfaces and capillaries in skeletal muscle from 3D confocal image
Keywords:
graph cut; skeletal muscle; confocal microscopy
Available at various institutes of the ASCR
Fitting geometrical models to 3D confocal microscopy images using graph cuts iteratively
A new iterative method for fitting triangulated surfaces and geometric graphs to surfaces and fibre-like structures is based on the calculation of the minimal graph cut in every iteration. The method ...
Elastic registration of biomedical images on CUDA-Supported graphics procesor units
Michálek, Jan; Čapek, Martin; Janáček, Jiří; Kubínová, Lucie
2010 - English
Elastic registration is a task of finding the matching of two images, using geometric and elastic transformations, so that objects in images have the same size, position and orientation. We apply elastic registration in the framework of volume reconstruction, where an object acquired from parallel physical sections is composed and mutual positions of the sections including deformations caused by their cutting have to be found. The method lies in optimizing a functional consisting of two parts: first, discrete total variation as a measure of roughness and, second, L1 norm as a measure of dissimilarity of images. As a parallelizable optimization strategy we apply a potential-based equivalent transformation of a (max,+)-labelling problem. CUDA-based implementation of the described elastic registration algorithm is reasonably fast, requires seconds to minutes of calculations, provides good results and, thus, can be used for practical tasks dealing with alignment of biomedical images
Keywords:
volume reconstruction; elastic alignment; CUDA
Available at various institutes of the ASCR
Elastic registration of biomedical images on CUDA-Supported graphics procesor units
Elastic registration is a task of finding the matching of two images, using geometric and elastic transformations, so that objects in images have the same size, position and orientation. We apply ...
Vliv genotypu a lokality na růst a vývoj kořenového systému řepky ozimé
Bláha, L.; Vyvadilová, M.; Janáček, Jiří
2009 - Czech
Z dosud získaných výsledků vyplývá, že kromě lokality má na růst a vývoj kořenové soustavy vliv také odolnost odrůd vůči abiotickým stresorům v době klíčení.Genotypy, které jsou klasifikované jako odolnější vůči stresorům již při klíčení za nepříznivých podmínek ( nedostatek vody, sucho, vysoká teplota), mají i kvalitnější kořenový systém po vysetí a během následujícího vegetačního období na testovaných lokalitách The basic aim of presented work was analysis of root development of different oilseed rape cultivars at two localities. Obtained results show large influence of localities on the root development. The genotypes with good germination under unfavourable conditions (lack of water, drought, high temperature) develop stronger root system i.e. volume and length of the roots after sowing and during following vegetation period at studying localities
Keywords:
winter oilseed rape; root system; stress tolerance
Available at various institutes of the ASCR
Vliv genotypu a lokality na růst a vývoj kořenového systému řepky ozimé
Z dosud získaných výsledků vyplývá, že kromě lokality má na růst a vývoj kořenové soustavy vliv také odolnost odrůd vůči abiotickým stresorům v době klíčení.Genotypy, které jsou klasifikované jako ...
Řešení problémů analýzy obrazu pomocí minima totální variance
Janáček, Jiří
2009 - Czech
Práce je věnována řešení úloh analýzy obrazu minimalizací funkce složené z totální variace obrazu a Lp ztrátové funkce. Minimalizaci lze provést iterační metodou největšího spádu s výpočtem kroku založeným na nalezení minimálního řezu v grafu obrazových prvkù. Jako příklad je uvedena regularizace zašuměného obrazu a registrace mikroskopických obrazů fyzických řezů A solution of various problems in image analysis using concurrent minimization of total variation and Lp loss function is presented. The minimization is achieved by a steepest descend method using graph cut minimization in each step. Regularization of noisy image and registration of microscopic images of physical sections are demonstrated
Keywords:
total variation; minimal graph cut
Available at various institutes of the ASCR
Řešení problémů analýzy obrazu pomocí minima totální variance
Práce je věnována řešení úloh analýzy obrazu minimalizací funkce složené z totální variace obrazu a Lp ztrátové funkce. Minimalizaci lze provést iterační metodou největšího spádu s výpočtem kroku ...
Multi-photon excitation microscopy
Chernyavskiy, Oleksandr; Kubínová, Lucie
2008 - English
Multiphoton excitation microscopy (MPM), being some time ago rather exotic than an easy-to-handle tool, becomes now more widely used due to advances in laser technique. This promising, yet expensive technique provides such advantages, as deeper penetration depth, lower photodamage, especially important in live cells imaging for extended periods of time Multifotonová excitační mikroskopie se z exotické techniky postupně stává stále více používaným nástrojem díky pokroku v laserové technice. Tato slibná i když náročná technika poskytuje výhody, jako je průnik do větší hloubky, nižší fotopoškození, jež jsou zvláště užitečné při dlouhodobém zobrazování buněk
Keywords:
two-photon excitation; multiphoton excitation; second-harmonic generation
Available at various institutes of the ASCR
Multi-photon excitation microscopy
Multiphoton excitation microscopy (MPM), being some time ago rather exotic than an easy-to-handle tool, becomes now more widely used due to advances in laser technique. This promising, yet expensive ...
Effects of dietary polyunsaturated fatty acids on myocardial and serum lipids in normoxic and chronically hypoxic rat heart
Balková, P.; Ježková, J.; Hlaváčková, M.; Neckář, Jan; Staňková, B.; Kolář, František; Novák, F.; Nováková, O.
2008 - English
The goal of this study was to fellow up the effect of n-3 and n-6 polyunsaturated fatty acids (PUFA) and the adaptation to chronic intermittent hypoxic (CIH) on fatty acid (FA) composition of serum and heart lipids in rats. The results of this study showed that PUFA diets and CIH distinctly modified FA profile in serum and heart lipids; both interventions considered to be cardioprotective Cílem práce bylo sledovat změny srdečních a sérových lipidů potkana po dlouhodobé dietě obohacené o n-3 a n-6 polynenasycené mastné kyseliny (PUFA) a adaptaci na chronickou intermitentní hypoxii (CIH). Studie ukázala, že jak dieta obohacená o PUFA tak CIH zásadně modifikují složení mastných kyselin v sledovaných lipidech a mohou souviset s potenciálními kardioprotektivními účinky obou intervencí
Keywords:
heart; chronic hypoxia; lipid diet
Available at various institutes of the ASCR
Effects of dietary polyunsaturated fatty acids on myocardial and serum lipids in normoxic and chronically hypoxic rat heart
The goal of this study was to fellow up the effect of n-3 and n-6 polyunsaturated fatty acids (PUFA) and the adaptation to chronic intermittent hypoxic (CIH) on fatty acid (FA) composition of serum ...
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